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The map label for this gene is amiC [H]

Identifier: 154248586

GI number: 154248586

Start: 5158940

End: 5160154

Strand: Reverse

Name: amiC [H]

Synonym: Xaut_4667

Alternate gene names: 154248586

Gene position: 5160154-5158940 (Counterclockwise)

Preceding gene: 154248592

Following gene: 154248582

Centisome position: 97.2

GC content: 70.37

Gene sequence:

>1215_bases
GTGGAGGCCGATCCGCCGGCGCAGGCCCATCCCAGTGCGGCCCCGCCGGCCGCTGGCGGCCCCGCCACCGCCAGCGATGC
CCGGCTTGCGGGCGACGCCCAGCGCACGCGCCTTGTGATCGACCTCGATCGCGCGGTGGAATTTCACGCCTTCACCCTGG
CCGAGCCCTATCGAGTGATCCTCGATTTGCCGGAGGTCGCCTTCGCGCTGCGGCCTGAGTCGAACCAGCTGAAGCGCGGA
TTGGTGTCTGCATACCGTTTCGGCGTGTTCGCGCCGGGCAAGGCACGCATGGTGCTGGAGGTGACCGGGCCGGTGGCCAT
CGACAAGGCCTTCGTACTCGATCCCGTGGACGACCAGCCCGCCCGCCTCGTGGTGGACCTGGTGAAGACTGATCCCGATA
CCTTCGCCCGCACGGCGGCGCGCTCCGCCGCCCAGCGCGTCGCGCCCCCCGTGACCCCCATCGTGCCGCCGGAGGCCGGG
GATGCCCGGCCGGTGGTGGTGCTCGATCCCGGCCATGGCGGCATCGATTCGGGCACCACGGGCTATTCCGCCTTCGCCGA
GAAGAATGTGGTGCTGGAGACCGCGCTCGCCGTGCGCGAGCTTCTGGAGCGTTCCGGCGCCTATCGGGTGGTGATGACGC
GCTCCACCGACACCTTCGTGCCGCTGGGGGAGCGGGTGCGCATCGCCCGCGCCCACCGCGCCGACCTGTTCGTGTCCTTC
CACGCCGATGCGCTGGCCCGCGGCGAGGGGCAGGCGCGGGGTGCCAGCGTCTACACCCTCGCCGAGACGGCGAGCGACGC
GGACGCTGCCCATCTCGCGGAAAAGGAGAACAAGGCCGACCTCATCGCCGGTGTCGACCTCTCCGACGAGACCGACGAGG
TGGCGGGCATCCTGGTGGACCTCGCCCAGCGCGAGACACGCAATTTCTCGGCCCTGCTCGCCCGCTCCCTGGTGGGCGCC
ATGAAGAGCACGGTGCGCTTTCACCGCTCGCCGCTGAAATCCGCGGGCTTCCGGGTGCTGCGCGCCCACGATGTGCCATC
GGTGCTGGTGGAGATCGGCTACATGTCCAGCAAGGAGGACCTGAAGCTGCTCACCTCGCCGGATGGCCGCGGACGCATCG
CCGAATCGGTGGTGGCGGGCATCAATCAGTTTTTCGCCCAGAAGGCGTCGGGCCTGCATGCCGCCGCCGACGACAAGGCA
GCCGTGCCGCGCTGA

Upstream 100 bases:

>100_bases
CGGGACAAGGCGCCGCAGCGGTTTGCGCGCGGTTTGCGGTGCCTTTTTTCTGGGCGTGCTCGCGGTCGCGTCATGCCTGG
CTCCCCCGCCGGCCCGCGCC

Downstream 100 bases:

>100_bases
GCTGAGCTGAGGCGCGGCCGGTCACAGCCCCGGCGAAGCCGCTTACAGCCCCCGGGCGATGACGATGCGCTGCACGTCGC
TGGTGCCCTCGTAGATCTGG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MEADPPAQAHPSAAPPAAGGPATASDARLAGDAQRTRLVIDLDRAVEFHAFTLAEPYRVILDLPEVAFALRPESNQLKRG
LVSAYRFGVFAPGKARMVLEVTGPVAIDKAFVLDPVDDQPARLVVDLVKTDPDTFARTAARSAAQRVAPPVTPIVPPEAG
DARPVVVLDPGHGGIDSGTTGYSAFAEKNVVLETALAVRELLERSGAYRVVMTRSTDTFVPLGERVRIARAHRADLFVSF
HADALARGEGQARGASVYTLAETASDADAAHLAEKENKADLIAGVDLSDETDEVAGILVDLAQRETRNFSALLARSLVGA
MKSTVRFHRSPLKSAGFRVLRAHDVPSVLVEIGYMSSKEDLKLLTSPDGRGRIAESVVAGINQFFAQKASGLHAAADDKA
AVPR

Sequences:

>Translated_404_residues
MEADPPAQAHPSAAPPAAGGPATASDARLAGDAQRTRLVIDLDRAVEFHAFTLAEPYRVILDLPEVAFALRPESNQLKRG
LVSAYRFGVFAPGKARMVLEVTGPVAIDKAFVLDPVDDQPARLVVDLVKTDPDTFARTAARSAAQRVAPPVTPIVPPEAG
DARPVVVLDPGHGGIDSGTTGYSAFAEKNVVLETALAVRELLERSGAYRVVMTRSTDTFVPLGERVRIARAHRADLFVSF
HADALARGEGQARGASVYTLAETASDADAAHLAEKENKADLIAGVDLSDETDEVAGILVDLAQRETRNFSALLARSLVGA
MKSTVRFHRSPLKSAGFRVLRAHDVPSVLVEIGYMSSKEDLKLLTSPDGRGRIAESVVAGINQFFAQKASGLHAAADDKA
AVPR
>Mature_404_residues
MEADPPAQAHPSAAPPAAGGPATASDARLAGDAQRTRLVIDLDRAVEFHAFTLAEPYRVILDLPEVAFALRPESNQLKRG
LVSAYRFGVFAPGKARMVLEVTGPVAIDKAFVLDPVDDQPARLVVDLVKTDPDTFARTAARSAAQRVAPPVTPIVPPEAG
DARPVVVLDPGHGGIDSGTTGYSAFAEKNVVLETALAVRELLERSGAYRVVMTRSTDTFVPLGERVRIARAHRADLFVSF
HADALARGEGQARGASVYTLAETASDADAAHLAEKENKADLIAGVDLSDETDEVAGILVDLAQRETRNFSALLARSLVGA
MKSTVRFHRSPLKSAGFRVLRAHDVPSVLVEIGYMSSKEDLKLLTSPDGRGRIAESVVAGINQFFAQKASGLHAAADDKA
AVPR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=369, Percent_Identity=34.4173441734417, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=40.8888888888889, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1790611, Length=258, Percent_Identity=30.2325581395349, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 42858; Mature: 42858

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEADPPAQAHPSAAPPAAGGPATASDARLAGDAQRTRLVIDLDRAVEFHAFTLAEPYRVI
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHEEEEEEECCCCEEEEEECCCCHHEE
LDLPEVAFALRPESNQLKRGLVSAYRFGVFAPGKARMVLEVTGPVAIDKAFVLDPVDDQP
EECCHHHEEECCCHHHHHHHHHHHHHHCEECCCCCEEEEEECCCEEECCEEEECCCCCCC
ARLVVDLVKTDPDTFARTAARSAAQRVAPPVTPIVPPEAGDARPVVVLDPGHGGIDSGTT
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
GYSAFAEKNVVLETALAVRELLERSGAYRVVMTRSTDTFVPLGERVRIARAHRADLFVSF
CHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEHHHHHCCEEEEEE
HADALARGEGQARGASVYTLAETASDADAAHLAEKENKADLIAGVDLSDETDEVAGILVD
CCHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHH
LAQRETRNFSALLARSLVGAMKSTVRFHRSPLKSAGFRVLRAHDVPSVLVEIGYMSSKED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCEEEEECCCCHHHHHHHCCCCCCCC
LKLLTSPDGRGRIAESVVAGINQFFAQKASGLHAAADDKAAVPR
CEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEADPPAQAHPSAAPPAAGGPATASDARLAGDAQRTRLVIDLDRAVEFHAFTLAEPYRVI
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHEEEEEEECCCCEEEEEECCCCHHEE
LDLPEVAFALRPESNQLKRGLVSAYRFGVFAPGKARMVLEVTGPVAIDKAFVLDPVDDQP
EECCHHHEEECCCHHHHHHHHHHHHHHCEECCCCCEEEEEECCCEEECCEEEECCCCCCC
ARLVVDLVKTDPDTFARTAARSAAQRVAPPVTPIVPPEAGDARPVVVLDPGHGGIDSGTT
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
GYSAFAEKNVVLETALAVRELLERSGAYRVVMTRSTDTFVPLGERVRIARAHRADLFVSF
CHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEHHHHHCCEEEEEE
HADALARGEGQARGASVYTLAETASDADAAHLAEKENKADLIAGVDLSDETDEVAGILVD
CCHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHH
LAQRETRNFSALLARSLVGAMKSTVRFHRSPLKSAGFRVLRAHDVPSVLVEIGYMSSKED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCEEEEECCCCHHHHHHHCCCCCCCC
LKLLTSPDGRGRIAESVVAGINQFFAQKASGLHAAADDKAAVPR
CEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]