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The map label for this gene is dacF [H]

Identifier: 154248459

GI number: 154248459

Start: 5016555

End: 5018546

Strand: Direct

Name: dacF [H]

Synonym: Xaut_4539

Alternate gene names: 154248459

Gene position: 5016555-5018546 (Clockwise)

Preceding gene: 154248455

Following gene: 154248462

Centisome position: 94.49

GC content: 69.43

Gene sequence:

>1992_bases
ATGCGTATATCGTCGGCCGGCCTCTCCATGACCTCGAAAGCCCTTGCTTTCGCGGTGCTTGCGGCGTTTTCCCTGACCGC
GACCGTGGCCGACGCAAAGCCGCGCAAGAAGGCCCATGTGGAGCGCTCAAAGGAGCGTTCCAAGAGCCGCACCGCACAGC
GCTCCACCCAGCCCGCGTCCGCCCAGGACAGCGAGGACGCCAGCCGCTGGAGGTATGGATCCTCCGCCATCATCGTGGAC
GCGAATACCGGCAAGGTGCTCTACGAGGATAATCCGGACGCCCTGCGACATCCCGCCTCAGTCACCAAGATCATGACGCT
TTACCTCCTGTTCGAGCAGCTGGAGGCAGGCAACCTGAAGCTGGACAGCGATCTTCAGGTTTCGGCCAAGGCGGCCTCGC
AGCAGCCCTCCAAGCTCGGCGTCAAGCCCGGCTCCGCCATCGAGGTGGAAGACGCCATCAAGGCGCTGGTGACACGCTCG
GCCAACGACGTGGCGGTGGTGATCGCGGAAAACCTCGGCGGCTCGGAATCCGAATTCGCGGAGATGATGACCCGCAAGGC
CCGCGCCCTGGGCATGAGCCGCACCGTGTTCCGCAACGCCTCCGGCCTGCCCAATCCCAACCAGGTGACCACGGCCCGCG
ACCTCTCCATCCTCGGGCGCGCGATCCAGGACAGGTTCCCCAAGCAATACAAGTATTTCGCCACCCGCACCTTCTATTAT
CGCGGGCAGGCCATCGGCAATCACAACCGCCTGCTCGGCCGGGTGGAGGGCGTGGACGGCATCAAGACCGGCTACACTCA
GGCCTCCGGCTTCAACCTCGTCACTTCGGTCAAGCGCGACGGGCGCTTTCTGGTGGGCGTGGTGCTCGGCGGCTCCAGCG
CCGGGGCGCGCGATGCGCGCATGACCAATCTCATCAGCCAGAACCTCAACGTCGCCTATGCCGGCGGGCGCACCGCGCCG
AAGGTCACCGAGGTGGCCGAGGCCGCCATTCCCGTGAACCGCTCGTTTGCCGCGCCCATGCCGGTGGTGGCCCAGCGCGA
CGACGACACGCCCCACGCCGTCGCCCCCCTGCCCAAGCCGGCCGCCGTGCAGATCGCCGCCGCCGAGCCGGCGACCACCG
CCTCCATTCCCCCGGCCAGCCACCGGAGCCAGAACCAGCAGCCCGCCACCCAGCAGGTGAAGCCCGGTTCCGCCGAACCC
ATCCGCCCGGTGGCGGTGAAGACGGTGGCGGTGCAGCGTCCGGTCCAGGCGTCGGCCACGACCACGGCCGTTGCCCCCTC
CCCGGCGAGCTTTGCCGCCCCCGCCGGCGTGCTCGGCTATCTCGGCCCCTCCGGCAACACCCTTTCGCCGGCCGCCTCTG
CGGTGCAGGCCGTGGCCCAAGGCCATGCCCGTCAGGCGCCGGCCAAGGAGGCTCCGGTCCAGACCGCGTCCCTTCAGCCC
GCAGCCGCCCCGCAGGCCGCAGCACCCACCGGCAAGGACACCTCGCGGCTTGCCTCGGCGGTCGCCAGCGGCGCCTCCGA
GCAGGCACTGCGCGCCGCCCGCATCGCCGCCATGGAACCGGACGACGCCTATGCCCAGGCCATGAAGGCCGCCCAGCGCA
CCCAGAGCGCGCCGCAGCAGGCAGCCGCCGCCCCGGCCCGGCCGGTGCGCACCGCCTCCCTCGTCCCGATGGCCGCCCCC
GAGCCGACAGCCACTTCTGCCACGGCCAAATCCGCCCCGGCGAAGTCCCCGGTGCCCGCCTCCGCCCATTCCGGCTGGCA
GATCCAGATCGGCGCCTTCGGTGCCGAGCGCGAGGCCAAGGCCAAGCTCGATGCCGCCAAGGCCCGCGCCAAGTCGGTGC
TCGGCAGCGCCGATCCCTATACCGAGAAGGTGACCAAGGGGTCGTCCGAACTCTACCGCGCCCGCTTTGCCGGGCTGGAT
GAAAAGGGCGCCCGCGAAGCCTGCCGTTTACTCAAGCGTAATGACTTCGACTGTATGACTTTCAAGAATTGA

Upstream 100 bases:

>100_bases
AACCCTCGGTGCGACGCACAAGCCTTACTCAAGGCTCCAGTGTCACAACGGGCGCGGGAACTGGCTCAGGTTTGCGACCA
GCGACACGGGACAAAAGTGC

Downstream 100 bases:

>100_bases
AGTCATGCACGGGACTCAGCCATGCTTGCGCAAGATCACGTCCTTGGCGCGATTTATCCTCGACGCGAGATAGCTGGATC
CGCCCAGGTCGGGGTGGAGC

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 663; Mature: 663

Protein sequence:

>663_residues
MRISSAGLSMTSKALAFAVLAAFSLTATVADAKPRKKAHVERSKERSKSRTAQRSTQPASAQDSEDASRWRYGSSAIIVD
ANTGKVLYEDNPDALRHPASVTKIMTLYLLFEQLEAGNLKLDSDLQVSAKAASQQPSKLGVKPGSAIEVEDAIKALVTRS
ANDVAVVIAENLGGSESEFAEMMTRKARALGMSRTVFRNASGLPNPNQVTTARDLSILGRAIQDRFPKQYKYFATRTFYY
RGQAIGNHNRLLGRVEGVDGIKTGYTQASGFNLVTSVKRDGRFLVGVVLGGSSAGARDARMTNLISQNLNVAYAGGRTAP
KVTEVAEAAIPVNRSFAAPMPVVAQRDDDTPHAVAPLPKPAAVQIAAAEPATTASIPPASHRSQNQQPATQQVKPGSAEP
IRPVAVKTVAVQRPVQASATTTAVAPSPASFAAPAGVLGYLGPSGNTLSPAASAVQAVAQGHARQAPAKEAPVQTASLQP
AAAPQAAAPTGKDTSRLASAVASGASEQALRAARIAAMEPDDAYAQAMKAAQRTQSAPQQAAAAPARPVRTASLVPMAAP
EPTATSATAKSAPAKSPVPASAHSGWQIQIGAFGAEREAKAKLDAAKARAKSVLGSADPYTEKVTKGSSELYRARFAGLD
EKGAREACRLLKRNDFDCMTFKN

Sequences:

>Translated_663_residues
MRISSAGLSMTSKALAFAVLAAFSLTATVADAKPRKKAHVERSKERSKSRTAQRSTQPASAQDSEDASRWRYGSSAIIVD
ANTGKVLYEDNPDALRHPASVTKIMTLYLLFEQLEAGNLKLDSDLQVSAKAASQQPSKLGVKPGSAIEVEDAIKALVTRS
ANDVAVVIAENLGGSESEFAEMMTRKARALGMSRTVFRNASGLPNPNQVTTARDLSILGRAIQDRFPKQYKYFATRTFYY
RGQAIGNHNRLLGRVEGVDGIKTGYTQASGFNLVTSVKRDGRFLVGVVLGGSSAGARDARMTNLISQNLNVAYAGGRTAP
KVTEVAEAAIPVNRSFAAPMPVVAQRDDDTPHAVAPLPKPAAVQIAAAEPATTASIPPASHRSQNQQPATQQVKPGSAEP
IRPVAVKTVAVQRPVQASATTTAVAPSPASFAAPAGVLGYLGPSGNTLSPAASAVQAVAQGHARQAPAKEAPVQTASLQP
AAAPQAAAPTGKDTSRLASAVASGASEQALRAARIAAMEPDDAYAQAMKAAQRTQSAPQQAAAAPARPVRTASLVPMAAP
EPTATSATAKSAPAKSPVPASAHSGWQIQIGAFGAEREAKAKLDAAKARAKSVLGSADPYTEKVTKGSSELYRARFAGLD
EKGAREACRLLKRNDFDCMTFKN
>Mature_663_residues
MRISSAGLSMTSKALAFAVLAAFSLTATVADAKPRKKAHVERSKERSKSRTAQRSTQPASAQDSEDASRWRYGSSAIIVD
ANTGKVLYEDNPDALRHPASVTKIMTLYLLFEQLEAGNLKLDSDLQVSAKAASQQPSKLGVKPGSAIEVEDAIKALVTRS
ANDVAVVIAENLGGSESEFAEMMTRKARALGMSRTVFRNASGLPNPNQVTTARDLSILGRAIQDRFPKQYKYFATRTFYY
RGQAIGNHNRLLGRVEGVDGIKTGYTQASGFNLVTSVKRDGRFLVGVVLGGSSAGARDARMTNLISQNLNVAYAGGRTAP
KVTEVAEAAIPVNRSFAAPMPVVAQRDDDTPHAVAPLPKPAAVQIAAAEPATTASIPPASHRSQNQQPATQQVKPGSAEP
IRPVAVKTVAVQRPVQASATTTAVAPSPASFAAPAGVLGYLGPSGNTLSPAASAVQAVAQGHARQAPAKEAPVQTASLQP
AAAPQAAAPTGKDTSRLASAVASGASEQALRAARIAAMEPDDAYAQAMKAAQRTQSAPQQAAAAPARPVRTASLVPMAAP
EPTATSATAKSAPAKSPVPASAHSGWQIQIGAFGAEREAKAKLDAAKARAKSVLGSADPYTEKVTKGSSELYRARFAGLD
EKGAREACRLLKRNDFDCMTFKN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=234, Percent_Identity=36.7521367521368, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786851, Length=242, Percent_Identity=33.0578512396694, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082022, Length=241, Percent_Identity=32.7800829875519, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87082054, Length=220, Percent_Identity=33.6363636363636, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 69144; Mature: 69144

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISSAGLSMTSKALAFAVLAAFSLTATVADAKPRKKAHVERSKERSKSRTAQRSTQPAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
AQDSEDASRWRYGSSAIIVDANTGKVLYEDNPDALRHPASVTKIMTLYLLFEQLEAGNLK
CCCCCHHHHHCCCCCEEEEECCCCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEE
LDSDLQVSAKAASQQPSKLGVKPGSAIEVEDAIKALVTRSANDVAVVIAENLGGSESEFA
ECCCCEEHHHHHCCCCHHCCCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHH
EMMTRKARALGMSRTVFRNASGLPNPNQVTTARDLSILGRAIQDRFPKQYKYFATRTFYY
HHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHEEEEEE
RGQAIGNHNRLLGRVEGVDGIKTGYTQASGFNLVTSVKRDGRFLVGVVLGGSSAGARDAR
ECCEECCCCHHEEEECCCCCCCCCCCCCCCCHHEEHHCCCCCEEEEEEECCCCCCCCHHH
MTNLISQNLNVAYAGGRTAPKVTEVAEAAIPVNRSFAAPMPVVAQRDDDTPHAVAPLPKP
HHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
AAVQIAAAEPATTASIPPASHRSQNQQPATQQVKPGSAEPIRPVAVKTVAVQRPVQASAT
CEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
TTAVAPSPASFAAPAGVLGYLGPSGNTLSPAASAVQAVAQGHARQAPAKEAPVQTASLQP
EEEECCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCC
AAAPQAAAPTGKDTSRLASAVASGASEQALRAARIAAMEPDDAYAQAMKAAQRTQSAPQQ
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHH
AAAAPARPVRTASLVPMAAPEPTATSATAKSAPAKSPVPASAHSGWQIQIGAFGAEREAK
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHH
AKLDAAKARAKSVLGSADPYTEKVTKGSSELYRARFAGLDEKGAREACRLLKRNDFDCMT
HHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
FKN
ECC
>Mature Secondary Structure
MRISSAGLSMTSKALAFAVLAAFSLTATVADAKPRKKAHVERSKERSKSRTAQRSTQPAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
AQDSEDASRWRYGSSAIIVDANTGKVLYEDNPDALRHPASVTKIMTLYLLFEQLEAGNLK
CCCCCHHHHHCCCCCEEEEECCCCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEE
LDSDLQVSAKAASQQPSKLGVKPGSAIEVEDAIKALVTRSANDVAVVIAENLGGSESEFA
ECCCCEEHHHHHCCCCHHCCCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHH
EMMTRKARALGMSRTVFRNASGLPNPNQVTTARDLSILGRAIQDRFPKQYKYFATRTFYY
HHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHEEEEEE
RGQAIGNHNRLLGRVEGVDGIKTGYTQASGFNLVTSVKRDGRFLVGVVLGGSSAGARDAR
ECCEECCCCHHEEEECCCCCCCCCCCCCCCCHHEEHHCCCCCEEEEEEECCCCCCCCHHH
MTNLISQNLNVAYAGGRTAPKVTEVAEAAIPVNRSFAAPMPVVAQRDDDTPHAVAPLPKP
HHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
AAVQIAAAEPATTASIPPASHRSQNQQPATQQVKPGSAEPIRPVAVKTVAVQRPVQASAT
CEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
TTAVAPSPASFAAPAGVLGYLGPSGNTLSPAASAVQAVAQGHARQAPAKEAPVQTASLQP
EEEECCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCC
AAAPQAAAPTGKDTSRLASAVASGASEQALRAARIAAMEPDDAYAQAMKAAQRTQSAPQQ
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHH
AAAAPARPVRTASLVPMAAPEPTATSATAKSAPAKSPVPASAHSGWQIQIGAFGAEREAK
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHH
AKLDAAKARAKSVLGSADPYTEKVTKGSSELYRARFAGLDEKGAREACRLLKRNDFDCMT
HHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
FKN
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]