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The map label for this gene is ntrC [H]

Identifier: 154248319

GI number: 154248319

Start: 4878480

End: 4879928

Strand: Reverse

Name: ntrC [H]

Synonym: Xaut_4399

Alternate gene names: 154248319

Gene position: 4879928-4878480 (Counterclockwise)

Preceding gene: 154248320

Following gene: 154248318

Centisome position: 91.92

GC content: 67.36

Gene sequence:

>1449_bases
GTGTCGATGCCTACCGGAAGCATCCTGGTCGCCGACGACGACGCCGCGATCCGCACCGTGCTCAACCAGGCGCTGTCGCG
CGCCGGCTACGAGGTGCGGTCCTGCGGCAACGCCGCCACACTCTGGCGCTGGGTGAGCCAGGGCGATGGCGACCTCGTCA
TCACCGATGTGGTGATGCCGGACGAGAACGCCTTCGATCTCCTGCCGCGCATCAAGAAGGCGCGGCCGGACCTGCCGGTC
ATCGTCATGAGCGCGCAGAACACCTTCATGACCGCCATCCGCGCTTCCGAGCGCGGGGCCTATGAATATCTGCCCAAGCC
GTTCGACCTGAAGGAGCTGATCTCCATCGTCGGCCGCGCCCTGGCAGAGCCGAAGAAGGGCGATGGCCACCACCATTCCC
ACGAGGAGCAGGACAATATCCCGCTGGTGGGCCGCTCGCCCGCCATGCAGGAGATCTATCGCCTCCTCGCCCGGCTGATG
CAGACCGACCTCACCGTGATGATCGCGGGCGAGAGCGGCACCGGCAAGGAGCTGGTGGCCCGTGCGCTCCATGATTACGG
CAAGCGCCGCACCGGGCCGTTCGTCGCCATCAACATGGCGGCGATCCCGCGCGACCTCATCGAATCGGAGCTGTTCGGCC
ACGAGAAGGGCTCGTTCACCGGCGCCACCACGCGCAATCCCGGCCGTTTCGAGCAGGCGGAGGGCGGCACGCTCTTCCTC
GACGAGATCGGCGACATGCCTATGGAGGCGCAGACCCGCCTGCTGCGCGTGCTGCAGCAGGGCGAATACACCACCGTCGG
CGGGCGTACGCCCATCAAGACCGACGTGCGCATCATCGCCGCCACCAACAAGGATTTGCGCACCCTGATCCAGCAGGGCC
TGTTCCGCGAGGACCTGTTCTTCCGCCTGAACGTGGTGCCTCTGCGCCTGCCGCCGTTGCGCGAGCGCTCGGAAGATGTG
CCGGACCTGGTGCGCCACTTCTTCCTCCAGGCGGAGCGGGAGGGCCTGCCGGCCAAGCAGATCGACACCGCCGCCCTCGA
CCGGCTGAAGCGCTACCGCTGGCCCGGCAACGTGCGCGAGCTGGAGAACCTGGTGCGGCGCCTCGCGGCGCTCTATCCGC
AGGAGGTGATCACCCCCGCCATCATCGAGGCGGAGCTGTCAGAGCCCATGCCGAGCGCCCTCTCGGAGGAGGGCCCGGTG
GATGCCACGCTGGCAGCCTCGGTGGAGCGTCACCTCAACACCTATTTCTCGGGCTTCGGCGAGGACCTGCCGCCCCCCGG
CCTCTATCACCGCATCCTGAAGGACGTGGAATACCCACTGCTTTCGGCGGCGCTGGCGGCTACGCGCGGCAACCAGATCA
AGGCCGCGGAGCTTCTGGGCCTGAACCGCAACACCCTGCGCAAGAAGATCCGGGATCTCGATATCCAGGTCATCCGCACG
TCCAGATGA

Upstream 100 bases:

>100_bases
GGCGGCATCGTCGAATGCGACAGTCAGCCCCGCCGTACAACCTTCCGCGTGCTCCTGCCCATGTATCGCGGCAAGAGCAC
GGCTCAAGAGGATAACTGAG

Downstream 100 bases:

>100_bases
TTCGGACCTCCAGATGATTCGGATATCCGCTAGGAGCGGGGAGGGCGGCTTCGGCCGCCTTCCGGTGTTTTTCGAATCGC
GCTTGGCGTCGCTTCGCCGC

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSMPTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMPDENAFDLLPRIKKARPDLPV
IVMSAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRALAEPKKGDGHHHSHEEQDNIPLVGRSPAMQEIYRLLARLM
QTDLTVMIAGESGTGKELVARALHDYGKRRTGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNPGRFEQAEGGTLFL
DEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLFFRLNVVPLRLPPLRERSEDV
PDLVRHFFLQAEREGLPAKQIDTAALDRLKRYRWPGNVRELENLVRRLAALYPQEVITPAIIEAELSEPMPSALSEEGPV
DATLAASVERHLNTYFSGFGEDLPPPGLYHRILKDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRT
SR

Sequences:

>Translated_482_residues
MSMPTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMPDENAFDLLPRIKKARPDLPV
IVMSAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRALAEPKKGDGHHHSHEEQDNIPLVGRSPAMQEIYRLLARLM
QTDLTVMIAGESGTGKELVARALHDYGKRRTGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNPGRFEQAEGGTLFL
DEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLFFRLNVVPLRLPPLRERSEDV
PDLVRHFFLQAEREGLPAKQIDTAALDRLKRYRWPGNVRELENLVRRLAALYPQEVITPAIIEAELSEPMPSALSEEGPV
DATLAASVERHLNTYFSGFGEDLPPPGLYHRILKDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRT
SR
>Mature_481_residues
SMPTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMPDENAFDLLPRIKKARPDLPVI
VMSAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRALAEPKKGDGHHHSHEEQDNIPLVGRSPAMQEIYRLLARLMQ
TDLTVMIAGESGTGKELVARALHDYGKRRTGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNPGRFEQAEGGTLFLD
EIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLFFRLNVVPLRLPPLRERSEDVP
DLVRHFFLQAEREGLPAKQIDTAALDRLKRYRWPGNVRELENLVRRLAALYPQEVITPAIIEAELSEPMPSALSEEGPVD
ATLAASVERHLNTYFSGFGEDLPPPGLYHRILKDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRTS
R

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=485, Percent_Identity=44.3298969072165, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1788550, Length=473, Percent_Identity=38.2663847780127, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1790437, Length=482, Percent_Identity=40.0414937759336, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1788905, Length=387, Percent_Identity=40.3100775193798, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI87082117, Length=255, Percent_Identity=49.8039215686275, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1789087, Length=335, Percent_Identity=43.5820895522388, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1789233, Length=346, Percent_Identity=41.907514450867, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=48.0519480519481, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI87081872, Length=343, Percent_Identity=39.6501457725948, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1786524, Length=291, Percent_Identity=42.6116838487972, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=245, Percent_Identity=40.8163265306122, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=35.6275303643725, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87081858, Length=245, Percent_Identity=32.2448979591837, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789809, Length=109, Percent_Identity=32.1100917431193, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786784, Length=108, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790552, Length=107, Percent_Identity=33.6448598130841, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53708; Mature: 53577

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMPTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMP
CCCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCEEEEEEECC
DENAFDLLPRIKKARPDLPVIVMSAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRA
CCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHH
LAEPKKGDGHHHSHEEQDNIPLVGRSPAMQEIYRLLARLMQTDLTVMIAGESGTGKELVA
HCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
RALHDYGKRRTGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNPGRFEQAEGGTLFL
HHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEH
DEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLF
HHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHE
FRLNVVPLRLPPLRERSEDVPDLVRHFFLQAEREGLPAKQIDTAALDRLKRYRWPGNVRE
EEEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHH
LENLVRRLAALYPQEVITPAIIEAELSEPMPSALSEEGPVDATLAASVERHLNTYFSGFG
HHHHHHHHHHHCCHHHHCHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
EDLPPPGLYHRILKDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHCCEEEEEC
SR
CC
>Mature Secondary Structure 
SMPTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMP
CCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCEEEEEEECC
DENAFDLLPRIKKARPDLPVIVMSAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRA
CCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHH
LAEPKKGDGHHHSHEEQDNIPLVGRSPAMQEIYRLLARLMQTDLTVMIAGESGTGKELVA
HCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
RALHDYGKRRTGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNPGRFEQAEGGTLFL
HHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEH
DEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLF
HHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHE
FRLNVVPLRLPPLRERSEDVPDLVRHFFLQAEREGLPAKQIDTAALDRLKRYRWPGNVRE
EEEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHH
LENLVRRLAALYPQEVITPAIIEAELSEPMPSALSEEGPVDATLAASVERHLNTYFSGFG
HHHHHHHHHHHCCHHHHCHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
EDLPPPGLYHRILKDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHCCEEEEEC
SR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]