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The map label for this gene is gltX

Identifier: 154248289

GI number: 154248289

Start: 4843501

End: 4844916

Strand: Reverse

Name: gltX

Synonym: Xaut_4369

Alternate gene names: 154248289

Gene position: 4844916-4843501 (Counterclockwise)

Preceding gene: 154248291

Following gene: 154248288

Centisome position: 91.26

GC content: 70.06

Gene sequence:

>1416_bases
ATGTCGCAGATCGTCACCCGCTTCGCCCCCTCCCCCACCGGCTTCCTGCACATCGGCGGGGCGCGCACGGCCCTGTTCAA
CTGGCTCTATGCCCGCCACACCGGCGGCAAGATGCTGCTGCGCATCGAGGACACCGACCGCCAGCGCTCCACCAAGGAGG
CGATCGCCGCCATCATCGAGGGGCTGGAGTGGCTGGGCATCACCTGCGACGGCGATCCCATCTACCAGTTCGCCCGCGCG
GAGCGGCATCGCGCCGCTGTTGAGGAGATGCTGGCCAAGGGCAATGCTTATCGCTGCTACGCCACGCCGCAGGAGCTGGA
CGAGATGCGCGAACTGGCCCGCAAGGAGGGCCGCCCGCCGCGCTATGACGGCCGCTGGCGCGACCGCGACCCGTCCGAGG
CGCCCAAGGATCGCGACCCCGTCATCCGCCTGCGCGCGCCGCAGGAGGGGGAGACCGTGATCGACGACATGGTGCAGGGC
ACCGTGACCTTCCCCAACAAGGATCTCGACGACCTCGTCCTGCTGCGCTCGGACGGCAATCCCACCTACATGCTGGCGGT
GGTGGTGGACGACCACGACATGGGCGTCACCCATGTGATCCGGGGCGACGACCACCTGACCAATGCGGCGCGCCAGACCC
AGATCTACCGCGCGCTGGGGTGGGAGGTGCCGCGCATGGCCCATATCCCGCTCATCCATGGGCCGGACGGCGCCAAGCTC
TCCAAGCGCCATGGCGCGCTGGGGGTAGATGCCTATCGCGACATGGGCTACCTGCCGGCGGCGCTGCGCAACTATCTGGT
GCGGCTCGGCTGGAGCCATGGCGACCAGGAAGTCTTCTCGACCGAGGAGATGGTCTCCTTCTTCGACCTCGACAAGGTGG
GCCGCTCCGCCGCGCGGTTCGATTTCCAGAAGCTGGAAAGCCTCAACGGCCATTACATGCGCGCCTCCAGCGATGCCGAG
CTGCTGTCGGCCATCGACGCGCTGGTGCCCCACCTGCCCGACGCCGAGCACCGCCTCGCCCGCATGACCCCGGAGCGCCG
CGCCCAGCTCGCCGCCGCCATGCCGGGGCTGAAGGAGCGCGCCAAGACGCTGGTGGAACTGGTGGACAACGCCGAGTTCA
TTTTCGTGGAGCGCCCGCTGCCGCTGGACGATAAGGCCGCCGCCCTGCTCTCGCCCGAGGCCCGCGCCCATCTCGCCCGC
CTCGTGCCGGCGCTGGAGCAGGTGGAGTGGACCGCCGCGGCCACCGAGGCTGCCGTGCGCGCCTATGCGGAGGCGCAGGG
TGTGAAGCTCGGCGCCGTGGCCCAGCCGCTGCGGGCCGCGCTCACCGGCAAGCCGACCTCGCCGCCCATCTTCGATGTGC
TCATGGTGCTGGGCCGGGACGAGAGCCTGGCGCGGCTGAAGGACCAGGCGGCTTGA

Upstream 100 bases:

>100_bases
TGAGGCTCGTGCTGGAGCCCCTGCCCCGGTCGACCCCGCGTACCGCCCGGGCGCTGCCCCGCCCCGTCCACCCCCTGGAC
CGAGCCTCGTCGAGTTCCCC

Downstream 100 bases:

>100_bases
AGGCGCGCCCTCCCGAGCCCGAACGGGTGGAGCTGGACCTCAGGGGGCTGAAATGCCCCCTGCCCGCCCTGCGTACCCGC
AAGGCGCTGAAGGCAGCGGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSQIVTRFAPSPTGFLHIGGARTALFNWLYARHTGGKMLLRIEDTDRQRSTKEAIAAIIEGLEWLGITCDGDPIYQFARA
ERHRAAVEEMLAKGNAYRCYATPQELDEMRELARKEGRPPRYDGRWRDRDPSEAPKDRDPVIRLRAPQEGETVIDDMVQG
TVTFPNKDLDDLVLLRSDGNPTYMLAVVVDDHDMGVTHVIRGDDHLTNAARQTQIYRALGWEVPRMAHIPLIHGPDGAKL
SKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEVFSTEEMVSFFDLDKVGRSAARFDFQKLESLNGHYMRASSDAE
LLSAIDALVPHLPDAEHRLARMTPERRAQLAAAMPGLKERAKTLVELVDNAEFIFVERPLPLDDKAAALLSPEARAHLAR
LVPALEQVEWTAAATEAAVRAYAEAQGVKLGAVAQPLRAALTGKPTSPPIFDVLMVLGRDESLARLKDQAA

Sequences:

>Translated_471_residues
MSQIVTRFAPSPTGFLHIGGARTALFNWLYARHTGGKMLLRIEDTDRQRSTKEAIAAIIEGLEWLGITCDGDPIYQFARA
ERHRAAVEEMLAKGNAYRCYATPQELDEMRELARKEGRPPRYDGRWRDRDPSEAPKDRDPVIRLRAPQEGETVIDDMVQG
TVTFPNKDLDDLVLLRSDGNPTYMLAVVVDDHDMGVTHVIRGDDHLTNAARQTQIYRALGWEVPRMAHIPLIHGPDGAKL
SKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEVFSTEEMVSFFDLDKVGRSAARFDFQKLESLNGHYMRASSDAE
LLSAIDALVPHLPDAEHRLARMTPERRAQLAAAMPGLKERAKTLVELVDNAEFIFVERPLPLDDKAAALLSPEARAHLAR
LVPALEQVEWTAAATEAAVRAYAEAQGVKLGAVAQPLRAALTGKPTSPPIFDVLMVLGRDESLARLKDQAA
>Mature_470_residues
SQIVTRFAPSPTGFLHIGGARTALFNWLYARHTGGKMLLRIEDTDRQRSTKEAIAAIIEGLEWLGITCDGDPIYQFARAE
RHRAAVEEMLAKGNAYRCYATPQELDEMRELARKEGRPPRYDGRWRDRDPSEAPKDRDPVIRLRAPQEGETVIDDMVQGT
VTFPNKDLDDLVLLRSDGNPTYMLAVVVDDHDMGVTHVIRGDDHLTNAARQTQIYRALGWEVPRMAHIPLIHGPDGAKLS
KRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEVFSTEEMVSFFDLDKVGRSAARFDFQKLESLNGHYMRASSDAEL
LSAIDALVPHLPDAEHRLARMTPERRAQLAAAMPGLKERAKTLVELVDNAEFIFVERPLPLDDKAAALLSPEARAHLARL
VPALEQVEWTAAATEAAVRAYAEAQGVKLGAVAQPLRAALTGKPTSPPIFDVLMVLGRDESLARLKDQAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=507, Percent_Identity=31.9526627218935, Blast_Score=228, Evalue=1e-59,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=45.2789699570815, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI226510925, Length=248, Percent_Identity=36.2903225806452, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1786895, Length=132, Percent_Identity=31.8181818181818, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=484, Percent_Identity=30.9917355371901, Blast_Score=204, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=486, Percent_Identity=33.9506172839506, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321192, Length=255, Percent_Identity=29.8039215686275, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=134, Percent_Identity=30.5970149253731, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=34.0122199592668, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_XANP2 (A7INJ6)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419247.1
- ProteinModelPortal:   A7INJ6
- SMR:   A7INJ6
- STRING:   A7INJ6
- GeneID:   5423565
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4369
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   XAUT78245:XAUT_4369-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52358; Mature: 52227

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIVTRFAPSPTGFLHIGGARTALFNWLYARHTGGKMLLRIEDTDRQRSTKEAIAAIIE
CCCCHHHHCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHH
GLEWLGITCDGDPIYQFARAERHRAAVEEMLAKGNAYRCYATPQELDEMRELARKEGRPP
CHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCC
RYDGRWRDRDPSEAPKDRDPVIRLRAPQEGETVIDDMVQGTVTFPNKDLDDLVLLRSDGN
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCEEECCCCCCCCEEEEECCCC
PTYMLAVVVDDHDMGVTHVIRGDDHLTNAARQTQIYRALGWEVPRMAHIPLIHGPDGAKL
CEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEECCCEEECCCCCCH
SKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEVFSTEEMVSFFDLDKVGRSAARF
HHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHH
DFQKLESLNGHYMRASSDAELLSAIDALVPHLPDAEHRLARMTPERRAQLAAAMPGLKER
HHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHH
AKTLVELVDNAEFIFVERPLPLDDKAAALLSPEARAHLARLVPALEQVEWTAAATEAAVR
HHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYAEAQGVKLGAVAQPLRAALTGKPTSPPIFDVLMVLGRDESLARLKDQAA
HHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure 
SQIVTRFAPSPTGFLHIGGARTALFNWLYARHTGGKMLLRIEDTDRQRSTKEAIAAIIE
CCCHHHHCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHH
GLEWLGITCDGDPIYQFARAERHRAAVEEMLAKGNAYRCYATPQELDEMRELARKEGRPP
CHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCC
RYDGRWRDRDPSEAPKDRDPVIRLRAPQEGETVIDDMVQGTVTFPNKDLDDLVLLRSDGN
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCEEECCCCCCCCEEEEECCCC
PTYMLAVVVDDHDMGVTHVIRGDDHLTNAARQTQIYRALGWEVPRMAHIPLIHGPDGAKL
CEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEECCCEEECCCCCCH
SKRHGALGVDAYRDMGYLPAALRNYLVRLGWSHGDQEVFSTEEMVSFFDLDKVGRSAARF
HHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHH
DFQKLESLNGHYMRASSDAELLSAIDALVPHLPDAEHRLARMTPERRAQLAAAMPGLKER
HHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHH
AKTLVELVDNAEFIFVERPLPLDDKAAALLSPEARAHLARLVPALEQVEWTAAATEAAVR
HHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYAEAQGVKLGAVAQPLRAALTGKPTSPPIFDVLMVLGRDESLARLKDQAA
HHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA