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The map label for this gene is lexA

Identifier: 154248291

GI number: 154248291

Start: 4847578

End: 4848291

Strand: Reverse

Name: lexA

Synonym: Xaut_4371

Alternate gene names: 154248291

Gene position: 4848291-4847578 (Counterclockwise)

Preceding gene: 154248292

Following gene: 154248289

Centisome position: 91.32

GC content: 67.93

Gene sequence:

>714_bases
ATGCTGACGCGCAAGCAATATGACCTGCTTCGCTTCATTCATGAGCGGCTGAAGGAGACCGGCGTGCCCCCCTCCTTCGA
CGAGATGAAGGAGGCGCTGGACCTGCGGTCCAAGTCCGGCATCCATCGCCTGATCACGGCACTGGAGGAGCGCGGCTTCA
TCCGCCGGCTGCCCAACCGGGCGCGCGCCCTCGAGGTGGTGCGCCTGCCGGACAGCGTCGCCCCCGGCCTCGCCACGCCC
CGCTCCGCCAGTCGCGGCTTCTCTCCCTCGGTGATCGAAGGCAGCCTCGGCAAGGTGCGTCCGGCTGCGGATGATGACGA
AGGCGCAGGCCAGGTGGTCACCGTTCCGGTTATGGGCCGGATTGCGGCGGGCTCGCCCATCTCCGCCATCCAGACCCGCA
GCCACACCCTCAACATTCCGCCTGAGATGCTGGGCAGCGGCGAGCATTTCGCCCTGGAGGTGCGCGGCGACAGCATGATC
GAGGCCGGCATCCTCGACGGTGACACGGTTCTCATCCGCAAGTGCGATACGGCGGACACCGGCGATATCATCGTGGCGCT
CGTGGACGACGAGGAAGCCACGCTGAAGCGCCTGCGCAAGAAGGGCGCCTCCATCGCCCTCGAAGCCGCCAATCCCGCCT
ACGAGACGCGCATCTTCGGGCCCGACCGGGTGCGCATCCAGGGCCGCCTCATCGGCCTCATGCGCCGCTACTGA

Upstream 100 bases:

>100_bases
TAACCCTGCCTTCACCCCTTGCGGAACACAAAAGGAACAGATAGAAACTGTTCATGATTTGTTTCGCGGCGCACAGGCGG
GCGCCAGCAGGGGGCGAGAG

Downstream 100 bases:

>100_bases
TTTCCGCTTCCTCCCCGGTGGGGAGGCCGCGACGGGGTGAGGGGCGTGGCGGTCACCATGCTCAACGGGACCGGCTCCTC
TGGAGCGACGCTTCTCACCC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNRARALEVVRLPDSVAPGLATP
RSASRGFSPSVIEGSLGKVRPAADDDEGAGQVVTVPVMGRIAAGSPISAIQTRSHTLNIPPEMLGSGEHFALEVRGDSMI
EAGILDGDTVLIRKCDTADTGDIIVALVDDEEATLKRLRKKGASIALEAANPAYETRIFGPDRVRIQGRLIGLMRRY

Sequences:

>Translated_237_residues
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNRARALEVVRLPDSVAPGLATP
RSASRGFSPSVIEGSLGKVRPAADDDEGAGQVVTVPVMGRIAAGSPISAIQTRSHTLNIPPEMLGSGEHFALEVRGDSMI
EAGILDGDTVLIRKCDTADTGDIIVALVDDEEATLKRLRKKGASIALEAANPAYETRIFGPDRVRIQGRLIGLMRRY
>Mature_237_residues
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNRARALEVVRLPDSVAPGLATP
RSASRGFSPSVIEGSLGKVRPAADDDEGAGQVVTVPVMGRIAAGSPISAIQTRSHTLNIPPEMLGSGEHFALEVRGDSMI
EAGILDGDTVLIRKCDTADTGDIIVALVDDEEATLKRLRKKGASIALEAANPAYETRIFGPDRVRIQGRLIGLMRRY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=236, Percent_Identity=27.5423728813559, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_XANP2 (A7INJ8)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419249.1
- ProteinModelPortal:   A7INJ8
- SMR:   A7INJ8
- STRING:   A7INJ8
- MEROPS:   S24.001
- GeneID:   5423567
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4371
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- ProtClustDB:   PRK00215
- BioCyc:   XAUT78245:XAUT_4371-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25887; Mature: 25887

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: ACT_SITE 158-158 ACT_SITE 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARALEVVRLPDSVAPGLATPRSASRGFSPSVIEGSLGKVRPAADDDEGAGQVVTVPVMGR
HHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECE
IAAGSPISAIQTRSHTLNIPPEMLGSGEHFALEVRGDSMIEAGILDGDTVLIRKCDTADT
ECCCCCHHHHHCCCCEECCCHHHHCCCCEEEEEECCCCEEEECCCCCCEEEEEECCCCCC
GDIIVALVDDEEATLKRLRKKGASIALEAANPAYETRIFGPDRVRIQGRLIGLMRRY
CCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCEEEECCCCEEEEHHHHHHHHHCC
>Mature Secondary Structure
MLTRKQYDLLRFIHERLKETGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARALEVVRLPDSVAPGLATPRSASRGFSPSVIEGSLGKVRPAADDDEGAGQVVTVPVMGR
HHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECE
IAAGSPISAIQTRSHTLNIPPEMLGSGEHFALEVRGDSMIEAGILDGDTVLIRKCDTADT
ECCCCCHHHHHCCCCEECCCHHHHCCCCEEEEEECCCCEEEECCCCCCEEEEEECCCCCC
GDIIVALVDDEEATLKRLRKKGASIALEAANPAYETRIFGPDRVRIQGRLIGLMRRY
CCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCEEEECCCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA