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The map label for this gene is mdeA [H]

Identifier: 154248253

GI number: 154248253

Start: 4806002

End: 4806610

Strand: Direct

Name: mdeA [H]

Synonym: Xaut_4333

Alternate gene names: 154248253

Gene position: 4806002-4806610 (Clockwise)

Preceding gene: 154248252

Following gene: 154248254

Centisome position: 90.53

GC content: 66.17

Gene sequence:

>609_bases
ATGCCGCCAGGGCTTCCAGCGCCAAACGCTCATCGACACCTGCTGTCACCGGCGACTTGCCCGCCATCACGCCCACCTGC
GAATCGACCACATCAGCGAATCGCAACATCATCCGTTGGGCAAGCCCCAGGAACCTGCTTAGGCCCGGCGCTGTCGCCCT
TCAACGCCTTCCTCATCCTCACGGGCATCGAGACGCTGGGGCTGCGCATGCAGCGGCACAGCGAGAATGCGCTGAAGGTG
GCCGAATATCTCGCCGCCCACCCCAAGGTGGAATGGGTGAGCTATCCGGGCCTTGCGAGCGACCGCTACCATGCCCTGGC
GAAGCAGTATCTGCCCAAGGGGGCGGGCGCGGTCTTCACCTTCGGCCTGAAGGGGGGCTACGAGGCGGGCGTGAAGCTGG
TGTCGAGCGTGGAGCTGTTCTCGCACCTCGCCAATATCGGCGACACGCGTTCCCTCATCATCCATCCGGCCTCCACCACC
CATCGCCAGCTCACCGACGAGGCCAAGGTGGTGGCCGGCGCCGGGCCGCAGGTGGTGCGCCTCTCGGTGGGCATCGAGGA
CGTGGCCGACATCATCGGCGACCTGGAGCAGGCACTGGCCAAGGTCTGA

Upstream 100 bases:

>100_bases
TCTTCGCGGACGCCAAACGCCTCCCCGATCCGCGCGCTGGTCCAGCCCGCCAGCGTCAGCAGCAACGCCCGCGCCCGGTC
CGCCTCGCCGCGCTCACGCG

Downstream 100 bases:

>100_bases
GGCTGCAGGAGCATTTTCGCGATACAGTCCGTCGCGAAAATGCTCCATGCTCCAGTTCGTTGCAGACCACGTCATCCCGA
AAAACAGGAAAGCCTGGATG

Product: Cys/Met metabolism pyridoxal-phosphate-dependent protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MPPGLPAPNAHRHLLSPATCPPSRPPANRPHQRIATSSVGQAPGTCLGPALSPFNAFLILTGIETLGLRMQRHSENALKV
AEYLAAHPKVEWVSYPGLASDRYHALAKQYLPKGAGAVFTFGLKGGYEAGVKLVSSVELFSHLANIGDTRSLIIHPASTT
HRQLTDEAKVVAGAGPQVVRLSVGIEDVADIIGDLEQALAKV

Sequences:

>Translated_202_residues
MPPGLPAPNAHRHLLSPATCPPSRPPANRPHQRIATSSVGQAPGTCLGPALSPFNAFLILTGIETLGLRMQRHSENALKV
AEYLAAHPKVEWVSYPGLASDRYHALAKQYLPKGAGAVFTFGLKGGYEAGVKLVSSVELFSHLANIGDTRSLIIHPASTT
HRQLTDEAKVVAGAGPQVVRLSVGIEDVADIIGDLEQALAKV
>Mature_201_residues
PPGLPAPNAHRHLLSPATCPPSRPPANRPHQRIATSSVGQAPGTCLGPALSPFNAFLILTGIETLGLRMQRHSENALKVA
EYLAAHPKVEWVSYPGLASDRYHALAKQYLPKGAGAVFTFGLKGGYEAGVKLVSSVELFSHLANIGDTRSLIIHPASTTH
RQLTDEAKVVAGAGPQVVRLSVGIEDVADIIGDLEQALAKV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI299473759, Length=153, Percent_Identity=37.2549019607843, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI21361334, Length=138, Percent_Identity=35.5072463768116, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI25453487, Length=138, Percent_Identity=35.5072463768116, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1790375, Length=146, Percent_Identity=34.2465753424658, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1789383, Length=166, Percent_Identity=31.3253012048193, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71980708, Length=155, Percent_Identity=47.0967741935484, Blast_Score=123, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17538055, Length=153, Percent_Identity=37.9084967320261, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560056, Length=154, Percent_Identity=37.6623376623377, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323333, Length=205, Percent_Identity=41.4634146341463, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319307, Length=159, Percent_Identity=38.9937106918239, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321254, Length=163, Percent_Identity=29.4478527607362, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922554, Length=149, Percent_Identity=39.5973154362416, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 21328; Mature: 21197

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPGLPAPNAHRHLLSPATCPPSRPPANRPHQRIATSSVGQAPGTCLGPALSPFNAFLIL
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
TGIETLGLRMQRHSENALKVAEYLAAHPKVEWVSYPGLASDRYHALAKQYLPKGAGAVFT
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEE
FGLKGGYEAGVKLVSSVELFSHLANIGDTRSLIIHPASTTHRQLTDEAKVVAGAGPQVVR
ECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHEEECCCCEEEE
LSVGIEDVADIIGDLEQALAKV
EECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PPGLPAPNAHRHLLSPATCPPSRPPANRPHQRIATSSVGQAPGTCLGPALSPFNAFLIL
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
TGIETLGLRMQRHSENALKVAEYLAAHPKVEWVSYPGLASDRYHALAKQYLPKGAGAVFT
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEE
FGLKGGYEAGVKLVSSVELFSHLANIGDTRSLIIHPASTTHRQLTDEAKVVAGAGPQVVR
ECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHEEECCCCEEEE
LSVGIEDVADIIGDLEQALAKV
EECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]