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The map label for this gene is mdeA [H]

Identifier: 154248252

GI number: 154248252

Start: 4804180

End: 4805583

Strand: Direct

Name: mdeA [H]

Synonym: Xaut_4332

Alternate gene names: 154248252

Gene position: 4804180-4805583 (Clockwise)

Preceding gene: 154248251

Following gene: 154248253

Centisome position: 90.49

GC content: 67.24

Gene sequence:

>1404_bases
ATGACCGACGAGACCAGCACGCCGCCGCACGAGCCGGGTTTTGCCACCCTCGCCATCCACGCGGGCGCGCAGCCGGACCC
GACCACGGGCGCACGGGCAACCCCTATCTACCAGACCACGTCCTTCGTCTTCGACGACGTGGACCATGCCGCGGCACTGT
TCGGCCTCCAGACCTTCGGCAACATCTATTCGCGCATCACCAACCCCACCAATGCGGTGCTGGAAGAGCGCGTGGCGGCG
CTGGAAGGCGGCACGGCGGCGCTGGCGGTGGCCTCGGGTCACGCGGCGCAGCACTTGGTGTTCCACACGCTGCTGACGCC
GGGGGATGAGTTCATCGCCTCCCGCAAGCTCTATGGCGGCTCCATCAACCAGTTCAACCACGCCTTCAAGAGCTTCGGCT
GGAACGTGGTGTGGGCGGATCCGGACGACATCTCCTCCTTCGAGAAGGCGGTGACGCCGAAGACCAAGGCCATCTTCACC
GAGAGCATCGCCAATCCCGGCGGCGTGGTCACCGACATCGCCGCCATCTCGGCGGTGGCGAAGAAGGCCGGCGTGCCGCT
CATCGTCGACAACACGCTGGCGACGCCCTACCTGTGGCAGCCCATCAGGCACGGCGCGGACATCGTCATCCATTCGGCCA
CCAAGTTTCTGGGCGGACACGGCAATTCCATCGGCGGCGTGATCGTGGACGCGGGCACATTCGACTGGTCCGCCACCGAT
CGCTATCCGTTCCTCTCCAAGCCGCGGCCGGAATATTCCGGCATGGTGCTGCACGAGACCTTCGGCAATTTCGCCTTCGC
CATCGCGGCCCGGGTGCTGGGCCTGCGCGATCTAAGCAGAGATTCCCCGTCTGGCCAACGAATGCGGCCTGCGCTGCTGG
TTGCGGGCGTGGACGGCGGCATGGATGGCGTGGTCGAGCGCGTCGCTATCCTTGAAAGTCTGGTGGGCGAGATGGTGCGC
CTTGAGGTCGCGCCAGACCGCTTCGATGTCGTTGAGCTCGGGCGCGTATTTCGGCAGCCATTCAACGGTGAGCCAGTGGG
CGCGGGCGGCCAGGGCGGCGCGGGAGAGCTTGCTGGTGTGGATCGGTCCGTTGTCCTCGACCAGCACGACGGGCCTGGCG
GCGCGGCCCGGCTTGGGGCCGTACGCCTCATCGAGTTGCTGGAGGTGGGCGACGAAATCGCTGCTGCGCTTGGTCGGGCT
GGTCTGGACGATCAGCTCCCGGGTGGCATGGTTGAGCGAGCCGAGCATCGCCACATTCTTGGCCTGACCCGGCGCCGGCA
CCCGCAGGTCGGCGCCGGACTTCGCCCAGGCACGGGCGAGGTAGGGATGGGTCAGCGCCTCGCTCTCGTCGCCATACAGC
AGCACGATGTCGCCTGCCGCGGCCTGCTGCTGGCGCAGTCGTAG

Upstream 100 bases:

>100_bases
GTGGACCTAAAATCTCATAACATCCGTCGATTTTCTGTATTAAATAAAAATCCACGCCGGGCTATCACGGATCGGCGTCA
GCCACTGGGAGAATGCGCTT

Downstream 100 bases:

>100_bases
GCGCAATCCGACCCGATCGATCTCGTCCGCCTTCTGGCGTCCCTTCAGCGTGTGCCGAGGCCGCCGCCAGCGGAACTTTT
TTTTCGCAGCGCCTTGGACA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTDETSTPPHEPGFATLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFGNIYSRITNPTNAVLEERVAA
LEGGTAALAVASGHAAQHLVFHTLLTPGDEFIASRKLYGGSINQFNHAFKSFGWNVVWADPDDISSFEKAVTPKTKAIFT
ESIANPGGVVTDIAAISAVAKKAGVPLIVDNTLATPYLWQPIRHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWSATD
RYPFLSKPRPEYSGMVLHETFGNFAFAIAARVLGLRDLSRDSPSGQRMRPALLVAGVDGGMDGVVERVAILESLVGEMVR
LEVAPDRFDVVELGRVFRQPFNGEPVGAGGQGGAGELAGVDRSVVLDQHDGPGGAARLGAVRLIELLEVGDEIAAALGRA
GLDDQLPGGMVERAEHRHILGLTRRRHPQVGAGLRPGTGEVGMGQRLALVAIQQHDVACRGLLLAQS

Sequences:

>Translated_467_residues
MTDETSTPPHEPGFATLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFGNIYSRITNPTNAVLEERVAA
LEGGTAALAVASGHAAQHLVFHTLLTPGDEFIASRKLYGGSINQFNHAFKSFGWNVVWADPDDISSFEKAVTPKTKAIFT
ESIANPGGVVTDIAAISAVAKKAGVPLIVDNTLATPYLWQPIRHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWSATD
RYPFLSKPRPEYSGMVLHETFGNFAFAIAARVLGLRDLSRDSPSGQRMRPALLVAGVDGGMDGVVERVAILESLVGEMVR
LEVAPDRFDVVELGRVFRQPFNGEPVGAGGQGGAGELAGVDRSVVLDQHDGPGGAARLGAVRLIELLEVGDEIAAALGRA
GLDDQLPGGMVERAEHRHILGLTRRRHPQVGAGLRPGTGEVGMGQRLALVAIQQHDVACRGLLLAQS
>Mature_466_residues
TDETSTPPHEPGFATLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFGNIYSRITNPTNAVLEERVAAL
EGGTAALAVASGHAAQHLVFHTLLTPGDEFIASRKLYGGSINQFNHAFKSFGWNVVWADPDDISSFEKAVTPKTKAIFTE
SIANPGGVVTDIAAISAVAKKAGVPLIVDNTLATPYLWQPIRHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWSATDR
YPFLSKPRPEYSGMVLHETFGNFAFAIAARVLGLRDLSRDSPSGQRMRPALLVAGVDGGMDGVVERVAILESLVGEMVRL
EVAPDRFDVVELGRVFRQPFNGEPVGAGGQGGAGELAGVDRSVVLDQHDGPGGAARLGAVRLIELLEVGDEIAAALGRAG
LDDQLPGGMVERAEHRHILGLTRRRHPQVGAGLRPGTGEVGMGQRLALVAIQQHDVACRGLLLAQS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=222, Percent_Identity=38.7387387387387, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI299473759, Length=222, Percent_Identity=31.981981981982, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI25453487, Length=221, Percent_Identity=31.6742081447964, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1790375, Length=215, Percent_Identity=35.3488372093023, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789383, Length=227, Percent_Identity=27.3127753303965, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560056, Length=219, Percent_Identity=37.4429223744292, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538055, Length=228, Percent_Identity=36.4035087719298, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71980708, Length=220, Percent_Identity=34.5454545454545, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323333, Length=293, Percent_Identity=47.098976109215, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6319307, Length=236, Percent_Identity=33.0508474576271, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=206, Percent_Identity=31.0679611650485, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321254, Length=234, Percent_Identity=31.1965811965812, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321904, Length=220, Percent_Identity=30, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922554, Length=224, Percent_Identity=33.9285714285714, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 49194; Mature: 49063

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDETSTPPHEPGFATLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFG
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
NIYSRITNPTNAVLEERVAALEGGTAALAVASGHAAQHLVFHTLLTPGDEFIASRKLYGG
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCC
SINQFNHAFKSFGWNVVWADPDDISSFEKAVTPKTKAIFTESIANPGGVVTDIAAISAVA
CHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
KKAGVPLIVDNTLATPYLWQPIRHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWSATD
HHCCCCEEECCCCCCCHHHHHHHCCCCEEEECHHHHHCCCCCCCCEEEEECCCCCCCCCC
RYPFLSKPRPEYSGMVLHETFGNFAFAIAARVLGLRDLSRDSPSGQRMRPALLVAGVDGG
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC
MDGVVERVAILESLVGEMVRLEVAPDRFDVVELGRVFRQPFNGEPVGAGGQGGAGELAGV
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DRSVVLDQHDGPGGAARLGAVRLIELLEVGDEIAAALGRAGLDDQLPGGMVERAEHRHIL
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHH
GLTRRRHPQVGAGLRPGTGEVGMGQRLALVAIQQHDVACRGLLLAQS
HHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHEEEEECC
>Mature Secondary Structure 
TDETSTPPHEPGFATLAIHAGAQPDPTTGARATPIYQTTSFVFDDVDHAAALFGLQTFG
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
NIYSRITNPTNAVLEERVAALEGGTAALAVASGHAAQHLVFHTLLTPGDEFIASRKLYGG
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCC
SINQFNHAFKSFGWNVVWADPDDISSFEKAVTPKTKAIFTESIANPGGVVTDIAAISAVA
CHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
KKAGVPLIVDNTLATPYLWQPIRHGADIVIHSATKFLGGHGNSIGGVIVDAGTFDWSATD
HHCCCCEEECCCCCCCHHHHHHHCCCCEEEECHHHHHCCCCCCCCEEEEECCCCCCCCCC
RYPFLSKPRPEYSGMVLHETFGNFAFAIAARVLGLRDLSRDSPSGQRMRPALLVAGVDGG
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC
MDGVVERVAILESLVGEMVRLEVAPDRFDVVELGRVFRQPFNGEPVGAGGQGGAGELAGV
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DRSVVLDQHDGPGGAARLGAVRLIELLEVGDEIAAALGRAGLDDQLPGGMVERAEHRHIL
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHH
GLTRRRHPQVGAGLRPGTGEVGMGQRLALVAIQQHDVACRGLLLAQS
HHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]