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The map label for this gene is eno

Identifier: 154248209

GI number: 154248209

Start: 4756522

End: 4757805

Strand: Direct

Name: eno

Synonym: Xaut_4289

Alternate gene names: 154248209

Gene position: 4756522-4757805 (Clockwise)

Preceding gene: 154248206

Following gene: 154248216

Centisome position: 89.59

GC content: 67.21

Gene sequence:

>1284_bases
ATGACCGCCATCGTGGACATCATCGGACGCGAAATTCTGGACAGCCGCGGCAATCCCACCGTGGAAGTGGACGTGGTGCT
GGAAGACGGCGCGCTCGGCCGCGCCGCCGTGCCCTCGGGCGCATCCACGGGCGCCCACGAGGCGGTGGAGCTGCGCGACG
GCGATGCTAGCCGCTATCTCGGCAAGGGCGTCGGCATGGCGGTGGATGCGGTCAACGGCGAGATCTTCGACGCCATCGGC
GGCTATGAGGCCGAGGACCAGGCCCACATCGACGCCGCCCTTCTCGCTCTCGACGGCACGCCCAACAAGGGCCGCCTGGG
CGCCAACGCCATCCTCGGCGTGTCGCTGGCGGTGGCCAAGGCCGCGGCCGAATCCAAGGGCCTGCCCCTCTACCGCTATG
TGGGCGGCGTGAACGCCCGCGTTCTGCCGGTGCCCATGATGAACATCATCAATGGCGGCGCCCACGCGGACAACCCCATC
GATTTCCAGGAATTCATGATCCTGCCCGCCGGCGCCCCCTCCTTCGCCGAGGGCCTGCGCTGGGGCGCGGAGATCTTCCA
CACCCTGAAGAAGGGTCTGAAGGACGCCGGTCACAACACCAATGTGGGCGACGAGGGCGGCTTCGCCCCCAACCTTGCCT
CCGCCGAGGCGGCGCTGGAATTCGTGCTCAAGGCCATCGAGAAGGCCGGCTTCAAGCCGGGCGAGGACGTGTATCTCGGC
CTCGACTGCGCCTCCACCGAGTTCTTCAAGAACGGCGTGTACAATTACGAGGGCGAGGGCACTGTGCGGGACATCGAGGC
CCAGGTGGCCTATCTCGCCGAACTGGTGGCCAAATATCCCATCGTCACCATCGAGGACGGCATGGCCGAGGACGACTGGG
TGGGCTGGAAGCTGCTCACCGACACGGTCGGCTCCAAGTGCCAGCTGGTGGGCGACGACCTGTTCGTCACCAATGTGGAG
CGCCTGTCGCGCGGCATCAAGGATGGCGTGGGCAACTCCATCCTGGTGAAGGTGAACCAGATCGGCTCCCTCACCGAGAC
GCTGGATGCGGTGGAAATGGCCCACAAGGCCGGCTATCGCGCCGTCATGTCCCACCGCTCGGGCGAGACCGAGGACGCGA
CCATCGCCGACCTGGCGGTGGCCACCAACTGCGGGCAGATCAAGACTGGCTCGCTGGCCCGCTCGGACCGCACCGCCAAG
TACAACCAGCTGCTGCGCATCGAGCAGGAGCTGGGCGACAGTGCCCGCTACGCCGGCAAGGCCGCGCTGAAGGCGCTGGC
CTGA

Upstream 100 bases:

>100_bases
CAAGGCTGGGGCTTTGGGCATCCGGGGTGCGGCAGGGCAGGGACGGCGACAGATCCTGTGTTTGTTGCGCCGCACCATTC
GACGCAGGGAAGGAGTCTTC

Downstream 100 bases:

>100_bases
GTCTCAAGCGACAGCCGCCCTCACCCGCTTGGGGTGAGGGCGGTTGCCCTCAGTCCGCCTTGTGCACCGCCACCGGGTCG
CTGGCCGGGAAGCTCTCCTC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAIVDIIGREILDSRGNPTVEVDVVLEDGALGRAAVPSGASTGAHEAVELRDGDASRYLGKGVGMAVDAVNGEIFDAIG
GYEAEDQAHIDAALLALDGTPNKGRLGANAILGVSLAVAKAAAESKGLPLYRYVGGVNARVLPVPMMNIINGGAHADNPI
DFQEFMILPAGAPSFAEGLRWGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALEFVLKAIEKAGFKPGEDVYLG
LDCASTEFFKNGVYNYEGEGTVRDIEAQVAYLAELVAKYPIVTIEDGMAEDDWVGWKLLTDTVGSKCQLVGDDLFVTNVE
RLSRGIKDGVGNSILVKVNQIGSLTETLDAVEMAHKAGYRAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRTAK
YNQLLRIEQELGDSARYAGKAALKALA

Sequences:

>Translated_427_residues
MTAIVDIIGREILDSRGNPTVEVDVVLEDGALGRAAVPSGASTGAHEAVELRDGDASRYLGKGVGMAVDAVNGEIFDAIG
GYEAEDQAHIDAALLALDGTPNKGRLGANAILGVSLAVAKAAAESKGLPLYRYVGGVNARVLPVPMMNIINGGAHADNPI
DFQEFMILPAGAPSFAEGLRWGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALEFVLKAIEKAGFKPGEDVYLG
LDCASTEFFKNGVYNYEGEGTVRDIEAQVAYLAELVAKYPIVTIEDGMAEDDWVGWKLLTDTVGSKCQLVGDDLFVTNVE
RLSRGIKDGVGNSILVKVNQIGSLTETLDAVEMAHKAGYRAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRTAK
YNQLLRIEQELGDSARYAGKAALKALA
>Mature_426_residues
TAIVDIIGREILDSRGNPTVEVDVVLEDGALGRAAVPSGASTGAHEAVELRDGDASRYLGKGVGMAVDAVNGEIFDAIGG
YEAEDQAHIDAALLALDGTPNKGRLGANAILGVSLAVAKAAAESKGLPLYRYVGGVNARVLPVPMMNIINGGAHADNPID
FQEFMILPAGAPSFAEGLRWGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALEFVLKAIEKAGFKPGEDVYLGL
DCASTEFFKNGVYNYEGEGTVRDIEAQVAYLAELVAKYPIVTIEDGMAEDDWVGWKLLTDTVGSKCQLVGDDLFVTNVER
LSRGIKDGVGNSILVKVNQIGSLTETLDAVEMAHKAGYRAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRTAKY
NQLLRIEQELGDSARYAGKAALKALA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=52.6806526806527, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=437, Percent_Identity=50.1144164759725, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=50.5747126436782, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=50.5747126436782, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=47.3441108545035, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.1791044776119, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.1791044776119, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.1791044776119, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789141, Length=432, Percent_Identity=59.7222222222222, Blast_Score=489, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=51.6203703703704, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=51.6203703703704, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=46.5608465608466, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=49.7685185185185, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=49.7685185185185, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=49.7685185185185, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.1916859122402, Blast_Score=381, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=48.8479262672811, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=48.3870967741936, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=48.3870967741936, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=48.3870967741936, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=48.3870967741936, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=48.8479262672811, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=48.8479262672811, Blast_Score=361, Evalue=1e-100,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_XANP2 (A7INB6)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419167.1
- ProteinModelPortal:   A7INB6
- SMR:   A7INB6
- STRING:   A7INB6
- GeneID:   5423673
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4289
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   XAUT78245:XAUT_4289-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44880; Mature: 44749

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVDIIGREILDSRGNPTVEVDVVLEDGALGRAAVPSGASTGAHEAVELRDGDASRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVGMAVDAVNGEIFDAIGGYEAEDQAHIDAALLALDGTPNKGRLGANAILGVSLAVAK
HCCCCEEEECCCCHHHHHCCCCCCCCHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAAESKGLPLYRYVGGVNARVLPVPMMNIINGGAHADNPIDFQEFMILPAGAPSFAEGLR
HHHHCCCCEEEHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
WGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALEFVLKAIEKAGFKPGEDVYLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCASTEFFKNGVYNYEGEGTVRDIEAQVAYLAELVAKYPIVTIEDGMAEDDWVGWKLLT
EECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEH
DTVGSKCQLVGDDLFVTNVERLSRGIKDGVGNSILVKVNQIGSLTETLDAVEMAHKAGYR
HHCCCCEEEECCCHHEECHHHHHHHHHHCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHH
AVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRTAKYNQLLRIEQELGDSARYAGK
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
AALKALA
HHHHHCC
>Mature Secondary Structure 
TAIVDIIGREILDSRGNPTVEVDVVLEDGALGRAAVPSGASTGAHEAVELRDGDASRYL
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVGMAVDAVNGEIFDAIGGYEAEDQAHIDAALLALDGTPNKGRLGANAILGVSLAVAK
HCCCCEEEECCCCHHHHHCCCCCCCCHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAAESKGLPLYRYVGGVNARVLPVPMMNIINGGAHADNPIDFQEFMILPAGAPSFAEGLR
HHHHCCCCEEEHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
WGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALEFVLKAIEKAGFKPGEDVYLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCASTEFFKNGVYNYEGEGTVRDIEAQVAYLAELVAKYPIVTIEDGMAEDDWVGWKLLT
EECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEH
DTVGSKCQLVGDDLFVTNVERLSRGIKDGVGNSILVKVNQIGSLTETLDAVEMAHKAGYR
HHCCCCEEEECCCHHEECHHHHHHHHHHCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHH
AVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRTAKYNQLLRIEQELGDSARYAGK
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
AALKALA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA