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The map label for this gene is paaF [C]

Identifier: 154248216

GI number: 154248216

Start: 4763830

End: 4764900

Strand: Direct

Name: paaF [C]

Synonym: Xaut_4296

Alternate gene names: 154248216

Gene position: 4763830-4764900 (Clockwise)

Preceding gene: 154248209

Following gene: 154248217

Centisome position: 89.73

GC content: 67.69

Gene sequence:

>1071_bases
GTGACAACGGAACCTGAGGTTCTGCTGGAAGAGAAGGGCGCAGCCGGCATCATCACGCTCAACCGGCCCAAGGCGCTCAA
CGCCTTGAACCTTGCCATGGTGCGCGAGATCCTGCCGCGCCTTCGGGCCTGGGCGAAGGATCCCGCCATCACCCGCGTCA
TCATCAAGGGTGCCGGCGAAAAGGCCTTCTGCGCCGGCGGTGACGTACGCTCCATCTACGACCTGGGCCTCTCCGGCCGC
GCCGAGGAAGCGCTCGGCTTCTTCCGCGAGGAATATGTCCTCAACGACTATATCGGCAGCTTTCCCAAGCCCTATGTGGC
GCTGATCGACGGCATCTGCATGGGCGGCGGCTTCGGCCTCTCGGCCCATGGTGCGCACCGGGTGGCGGGCGACCGCTACC
TGTTCGCCATGCCGGAGGTGAATATCGGCCTGTTCCCCGATGTGGGCGGCACCCATGTGCTGCCGCGCCTGCCCGGCGCC
TTCGGCGTGTTCCTGGCCCTGACCGGGACGCGAATCCGCGCCGCCGAGGCTCATGCCTGCGGGCTCGCCACCGACGTGGT
TCCCTCTGCCGCCTTCCCCGCATTGGAGGAGGCTCTCGTGGCAGGCGGCGACGTGGAGCGGATTCTCGCCGAGCATCGTA
TCGATCCGGGGCCGGGGACATTCGCCAGCCGCGCCGATTTCATCGCCGACACGTTCGGCCGCCCCGATGTGCCGGCCATC
CTCGCCGCCCTCGATGACGCTGCGGTGGCCGGTGGGGAGCATGGGGAATTCGCCGCAGCCACCGCCCGCGAGGTGCGCCT
GCGTTCCCCCACCAGCCTGTGCATCGCAATGGAGCAGATGCGCCGGGGCCGGGCGCTGGATCTTGGCGCCTGCCTGAAGC
TGGAACTGCGCGTGGTGACGCGCGTCATGGCCGGCCACGACTTCTACGAGGGCGTGCGCGCGGTGCTGGTGGACCGCGAC
AATGCCCCGAAATGGCAGCCGTCAAAGCTCGAAGAGGTGGACAGCGCCGTCATTGCGGCGCATTTCGATCCCATCCCCAA
CGAACTGGAATTACTGGCACCGGGTGCATGA

Upstream 100 bases:

>100_bases
GGTGGAGGTGCGCGCAGTCGCGTTGACGGTCCGCAGGGCCACGTCTAACCCTGCCCGGGGAACCGCTCAGCGGCAATCGG
CGGCGAAGGGGAGAGGCGCA

Downstream 100 bases:

>100_bases
GCCATTACGATCCCATCGATGCCAGCGCCCGCGCCCAGTGGGAAAACCTGACGCCCTGGCGGCGGCGGCTGTTCCTGTTC
CTGCGCGGCGTCGCTGCGTT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTTEPEVLLEEKGAAGIITLNRPKALNALNLAMVREILPRLRAWAKDPAITRVIIKGAGEKAFCAGGDVRSIYDLGLSGR
AEEALGFFREEYVLNDYIGSFPKPYVALIDGICMGGGFGLSAHGAHRVAGDRYLFAMPEVNIGLFPDVGGTHVLPRLPGA
FGVFLALTGTRIRAAEAHACGLATDVVPSAAFPALEEALVAGGDVERILAEHRIDPGPGTFASRADFIADTFGRPDVPAI
LAALDDAAVAGGEHGEFAAATAREVRLRSPTSLCIAMEQMRRGRALDLGACLKLELRVVTRVMAGHDFYEGVRAVLVDRD
NAPKWQPSKLEEVDSAVIAAHFDPIPNELELLAPGA

Sequences:

>Translated_356_residues
MTTEPEVLLEEKGAAGIITLNRPKALNALNLAMVREILPRLRAWAKDPAITRVIIKGAGEKAFCAGGDVRSIYDLGLSGR
AEEALGFFREEYVLNDYIGSFPKPYVALIDGICMGGGFGLSAHGAHRVAGDRYLFAMPEVNIGLFPDVGGTHVLPRLPGA
FGVFLALTGTRIRAAEAHACGLATDVVPSAAFPALEEALVAGGDVERILAEHRIDPGPGTFASRADFIADTFGRPDVPAI
LAALDDAAVAGGEHGEFAAATAREVRLRSPTSLCIAMEQMRRGRALDLGACLKLELRVVTRVMAGHDFYEGVRAVLVDRD
NAPKWQPSKLEEVDSAVIAAHFDPIPNELELLAPGA
>Mature_355_residues
TTEPEVLLEEKGAAGIITLNRPKALNALNLAMVREILPRLRAWAKDPAITRVIIKGAGEKAFCAGGDVRSIYDLGLSGRA
EEALGFFREEYVLNDYIGSFPKPYVALIDGICMGGGFGLSAHGAHRVAGDRYLFAMPEVNIGLFPDVGGTHVLPRLPGAF
GVFLALTGTRIRAAEAHACGLATDVVPSAAFPALEEALVAGGDVERILAEHRIDPGPGTFASRADFIADTFGRPDVPAIL
AALDDAAVAGGEHGEFAAATAREVRLRSPTSLCIAMEQMRRGRALDLGACLKLELRVVTRVMAGHDFYEGVRAVLVDRDN
APKWQPSKLEEVDSAVIAAHFDPIPNELELLAPGA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=352, Percent_Identity=45.7386363636364, Blast_Score=298, Evalue=4e-81,
Organism=Homo sapiens, GI37594469, Length=310, Percent_Identity=44.8387096774194, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI194097323, Length=213, Percent_Identity=31.924882629108, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI45643119, Length=193, Percent_Identity=30.5699481865285, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI260275230, Length=193, Percent_Identity=30.5699481865285, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI260274832, Length=193, Percent_Identity=30.5699481865285, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI70995211, Length=183, Percent_Identity=31.1475409836066, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=34.2541436464088, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI31542718, Length=173, Percent_Identity=32.3699421965318, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI20127408, Length=181, Percent_Identity=28.7292817679558, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=192, Percent_Identity=31.25, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI221142681, Length=228, Percent_Identity=32.0175438596491, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=181, Percent_Identity=34.2541436464088, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788597, Length=188, Percent_Identity=30.8510638297872, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787660, Length=180, Percent_Identity=34.4444444444444, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=362, Percent_Identity=43.0939226519337, Blast_Score=284, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI25144157, Length=362, Percent_Identity=43.0939226519337, Blast_Score=284, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI25145438, Length=187, Percent_Identity=34.2245989304813, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=181, Percent_Identity=32.0441988950276, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=187, Percent_Identity=31.0160427807487, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=189, Percent_Identity=32.2751322751323, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=189, Percent_Identity=32.2751322751323, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=183, Percent_Identity=32.2404371584699, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=29.4117647058824, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=192, Percent_Identity=31.25, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=180, Percent_Identity=28.8888888888889, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=380, Percent_Identity=30, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28571730, Length=349, Percent_Identity=44.4126074498567, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI28571729, Length=349, Percent_Identity=44.4126074498567, Blast_Score=287, Evalue=6e-78,
Organism=Drosophila melanogaster, GI20129971, Length=181, Percent_Identity=36.4640883977901, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653477, Length=181, Percent_Identity=36.4640883977901, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920382, Length=181, Percent_Identity=30.3867403314917, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24653139, Length=225, Percent_Identity=30.6666666666667, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 37825; Mature: 37694

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEPEVLLEEKGAAGIITLNRPKALNALNLAMVREILPRLRAWAKDPAITRVIIKGAGE
CCCCCCHHEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
KAFCAGGDVRSIYDLGLSGRAEEALGFFREEYVLNDYIGSFPKPYVALIDGICMGGGFGL
CEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
SAHGAHRVAGDRYLFAMPEVNIGLFPDVGGTHVLPRLPGAFGVFLALTGTRIRAAEAHAC
CCCCCHHCCCCEEEEECCCCCCEECCCCCCCCCCCCCCCHHEEHHHCCCCEEEECCHHHC
GLATDVVPSAAFPALEEALVAGGDVERILAEHRIDPGPGTFASRADFIADTFGRPDVPAI
CCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHH
LAALDDAAVAGGEHGEFAAATAREVRLRSPTSLCIAMEQMRRGRALDLGACLKLELRVVT
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
RVMAGHDFYEGVRAVLVDRDNAPKWQPSKLEEVDSAVIAAHFDPIPNELELLAPGA
HHHHCCHHHHHHHEEEEECCCCCCCCCHHHHHHHHHEEEEECCCCCCCEEEECCCC
>Mature Secondary Structure 
TTEPEVLLEEKGAAGIITLNRPKALNALNLAMVREILPRLRAWAKDPAITRVIIKGAGE
CCCCCHHEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
KAFCAGGDVRSIYDLGLSGRAEEALGFFREEYVLNDYIGSFPKPYVALIDGICMGGGFGL
CEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
SAHGAHRVAGDRYLFAMPEVNIGLFPDVGGTHVLPRLPGAFGVFLALTGTRIRAAEAHAC
CCCCCHHCCCCEEEEECCCCCCEECCCCCCCCCCCCCCCHHEEHHHCCCCEEEECCHHHC
GLATDVVPSAAFPALEEALVAGGDVERILAEHRIDPGPGTFASRADFIADTFGRPDVPAI
CCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHH
LAALDDAAVAGGEHGEFAAATAREVRLRSPTSLCIAMEQMRRGRALDLGACLKLELRVVT
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
RVMAGHDFYEGVRAVLVDRDNAPKWQPSKLEEVDSAVIAAHFDPIPNELELLAPGA
HHHHCCHHHHHHHEEEEECCCCCCCCCHHHHHHHHHEEEEECCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA