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The map label for this gene is tgt [H]

Identifier: 154248191

GI number: 154248191

Start: 4737590

End: 4738744

Strand: Direct

Name: tgt [H]

Synonym: Xaut_4271

Alternate gene names: 154248191

Gene position: 4737590-4738744 (Clockwise)

Preceding gene: 154248190

Following gene: 154248192

Centisome position: 89.24

GC content: 68.57

Gene sequence:

>1155_bases
ATGACCGCCCACACTCCTGTCGACACCTTCTCCTTCACCCTGTCCGCCACCGACGGCGCCGCCCGCACCGGCTTCGTCTC
GACCCCGCGCGGGGCCATCCGCACACCGGCCTTCATGCCGGTGGGCACGGCGGCCACCGTGAAGGGGCTCTATTTCGACC
AGGTGAAGCAGACGGGCGCCGACATCGTCCTCGCCAACACCTACCACCTCATGCTGCGCCCTGGCGCCGAGCGGATCGCA
AAGCTCGGCGGGCTGCACAGCTTCATGCGCTGGGACGGGCCGATCCTTACCGATTCCGGCGGCTTCCAGGTGATGAGCCT
GTCGAGCCTGCGCAAGATGAGCGAGCAGGGCGTCACCTTCCGCTCCCATGTGGACGGCGCCTACTTCGAGATGAGCCCGG
AGCGCTCGGTGGAGATCCAGGGACTGCTCGGCGCCGATATCCAGATGCAGCTGGACGAATGCGTGCGCCTGCCCTGCACC
GATGAAGAGGCCGCGCGCGCCATGCGCCTGTCACTGCGCTGGGCCGAGCGCTCGAAGCGCGCGTTCGAGGCGCAGCCGAA
GGGCCGGGCGCTGTTCGGCATCGTGCAGGGCGGGGCGGTGCCGGCGCTGCGGGTGGAATCGGCCCGCGCGCTGGCGGACA
TGGACTTCCCCGGCTATTCCATCGGCGGCCTTGCGGTGGGCGAGCCGCAGGAGGTCATGCTCGCCATGATCGAGACGGTG
GAGCCGCATCTGCCGGTCAACAAGCCGCGCTACCTGATGGGTGTGGGCACGCCGGACGACCTTCTGGAATCGGTGGCGCG
CGGCATCGACATGTTCGACTGCGTGATGCCCACCCGCTCCGGCCGCCACGCTTTGGCCTTCACCCGCTTCGGCCGCATCA
ACCTGAAGAACGCCCGCCACGCCGACGACCCCCGTCCGCTGGACGAGGAAAGCGACTGCCCGGCCCTGCGCGACTATTCC
CGCGCCTACCTCCACCACCTCGTGCGCTGCGAGGAGATGCTAGGCTCCATGCTGCTCACCTGGGCCAATGTCCATTATTA
CGAGCAGCTCATGGCCGGTGCCCGCGCCGCCATCGCCGAGGGGCGGTACGCCGATTATATGGCGCAGGTGAAGGACGGCT
GGGCGCGGGGAGATATTCCCGTGTGGGCAGGGTAG

Upstream 100 bases:

>100_bases
GGCACGAGGCTCAGAAGCGCGCCTATGCCCATGCCATCGCGGCCGGCTATCGCTTCTATTCCTATGGCGATGCGTGCCTG
CTTCTGCCAGAAGGCGGGCC

Downstream 100 bases:

>100_bases
GGATGTGCCCCGGACAAGCGACGGTGCAGCCGGAGCGCTGATCCGGGGAACCAAAGACTCCCGCCCAACCCCATTGAATA
AACGGACAACCGTTTTATTC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTAHTPVDTFSFTLSATDGAARTGFVSTPRGAIRTPAFMPVGTAATVKGLYFDQVKQTGADIVLANTYHLMLRPGAERIA
KLGGLHSFMRWDGPILTDSGGFQVMSLSSLRKMSEQGVTFRSHVDGAYFEMSPERSVEIQGLLGADIQMQLDECVRLPCT
DEEAARAMRLSLRWAERSKRAFEAQPKGRALFGIVQGGAVPALRVESARALADMDFPGYSIGGLAVGEPQEVMLAMIETV
EPHLPVNKPRYLMGVGTPDDLLESVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARHADDPRPLDEESDCPALRDYS
RAYLHHLVRCEEMLGSMLLTWANVHYYEQLMAGARAAIAEGRYADYMAQVKDGWARGDIPVWAG

Sequences:

>Translated_384_residues
MTAHTPVDTFSFTLSATDGAARTGFVSTPRGAIRTPAFMPVGTAATVKGLYFDQVKQTGADIVLANTYHLMLRPGAERIA
KLGGLHSFMRWDGPILTDSGGFQVMSLSSLRKMSEQGVTFRSHVDGAYFEMSPERSVEIQGLLGADIQMQLDECVRLPCT
DEEAARAMRLSLRWAERSKRAFEAQPKGRALFGIVQGGAVPALRVESARALADMDFPGYSIGGLAVGEPQEVMLAMIETV
EPHLPVNKPRYLMGVGTPDDLLESVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARHADDPRPLDEESDCPALRDYS
RAYLHHLVRCEEMLGSMLLTWANVHYYEQLMAGARAAIAEGRYADYMAQVKDGWARGDIPVWAG
>Mature_383_residues
TAHTPVDTFSFTLSATDGAARTGFVSTPRGAIRTPAFMPVGTAATVKGLYFDQVKQTGADIVLANTYHLMLRPGAERIAK
LGGLHSFMRWDGPILTDSGGFQVMSLSSLRKMSEQGVTFRSHVDGAYFEMSPERSVEIQGLLGADIQMQLDECVRLPCTD
EEAARAMRLSLRWAERSKRAFEAQPKGRALFGIVQGGAVPALRVESARALADMDFPGYSIGGLAVGEPQEVMLAMIETVE
PHLPVNKPRYLMGVGTPDDLLESVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARHADDPRPLDEESDCPALRDYSR
AYLHHLVRCEEMLGSMLLTWANVHYYEQLMAGARAAIAEGRYADYMAQVKDGWARGDIPVWAG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=368, Percent_Identity=44.2934782608696, Blast_Score=295, Evalue=6e-80,
Organism=Escherichia coli, GI1786607, Length=358, Percent_Identity=52.5139664804469, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17544680, Length=359, Percent_Identity=40.3899721448468, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI19920510, Length=384, Percent_Identity=41.6666666666667, Blast_Score=298, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42190; Mature: 42059

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHTPVDTFSFTLSATDGAARTGFVSTPRGAIRTPAFMPVGTAATVKGLYFDQVKQTGA
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DIVLANTYHLMLRPGAERIAKLGGLHSFMRWDGPILTDSGGFQVMSLSSLRKMSEQGVTF
CEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEE
RSHVDGAYFEMSPERSVEIQGLLGADIQMQLDECVRLPCTDEEAARAMRLSLRWAERSKR
ECCCCCEEEEECCCCCEEEEEEECCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AFEAQPKGRALFGIVQGGAVPALRVESARALADMDFPGYSIGGLAVGEPQEVMLAMIETV
HHCCCCCCCEEEEEECCCCCCEEEEHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHC
EPHLPVNKPRYLMGVGTPDDLLESVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARH
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEECCEEECCCCC
ADDPRPLDEESDCPALRDYSRAYLHHLVRCEEMLGSMLLTWANVHYYEQLMAGARAAIAE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRYADYMAQVKDGWARGDIPVWAG
CHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TAHTPVDTFSFTLSATDGAARTGFVSTPRGAIRTPAFMPVGTAATVKGLYFDQVKQTGA
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DIVLANTYHLMLRPGAERIAKLGGLHSFMRWDGPILTDSGGFQVMSLSSLRKMSEQGVTF
CEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEE
RSHVDGAYFEMSPERSVEIQGLLGADIQMQLDECVRLPCTDEEAARAMRLSLRWAERSKR
ECCCCCEEEEECCCCCEEEEEEECCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AFEAQPKGRALFGIVQGGAVPALRVESARALADMDFPGYSIGGLAVGEPQEVMLAMIETV
HHCCCCCCCEEEEEECCCCCCEEEEHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHC
EPHLPVNKPRYLMGVGTPDDLLESVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARH
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEECCEEECCCCC
ADDPRPLDEESDCPALRDYSRAYLHHLVRCEEMLGSMLLTWANVHYYEQLMAGARAAIAE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRYADYMAQVKDGWARGDIPVWAG
CHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]