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The map label for this gene is dctD [H]

Identifier: 154247969

GI number: 154247969

Start: 4478018

End: 4479397

Strand: Reverse

Name: dctD [H]

Synonym: Xaut_4048

Alternate gene names: 154247969

Gene position: 4479397-4478018 (Counterclockwise)

Preceding gene: 154247970

Following gene: 154247968

Centisome position: 84.37

GC content: 70.51

Gene sequence:

>1380_bases
ATGTGTGCTGAGCGCGCCCTCAAGTCCACTCCCGCCCCGCACCCGGCCGCCCGGCCCCGCGTGGTGCTGGTGGACGACGA
GGAGATGATCCGCATCACCATCGAGCAGACGCTGGAACTGGCCGGCATCGCGGTCGAGACCTTCGACAGCGTGGATGCGG
CCCTGCCCGCCATCGGCCGCGACTTTCCCGGCGTGGTGGTGAGCGACGTGCGCATGCCGGTGCGCGACGGGCTGGAACTG
CTGGCGGACGTGCGCCGGCGCGATCCCGAATTGCCGGTGGTGCTCATCACCGGGCATGGGGACGTGGCCATGGCGGTAAG
CGCCATGCGCGAGGGCGCCTATGATTTCATCGAGAAGCCGTTCGTCTCCGACGCCTTCGTGGAGGTGGTGCGCCGCGCCC
TCGACAAGCGCGCCCTCGTGATGGAAAACCGCCGCCTGCGTATGGCGCTGGACCGGGGCGAGGCGGGCGACGCCATCGAG
CGCTGCCTCGTCGGCCAGTCCCCCGCCATCCGCCGGCTGCGGGACGACGTGGCGCAGCTCGCCTCCACCAGTGCCGACGT
GCTGGTGCTGGGCGAGACCGGCGCCGGCAAGGAGCAGGTGGCCCGCGCCCTGCACGAGGGCGGATCGCGGCGCGACAAGC
CGTTCGTGGCGGTCAATTGCGGCGCCATCCCCGAAAGCATGTTCGAGAGCGAGATGTTCCGCCACGAGGCCGGCGCCTTC
ACCGGTGCCGGCAAGAAGCGCATCGGCAAGATCGAGCACGCGAGCGGAGGCACCCTCTTCCTCGACGAGGTGGAGAGCAT
GCCGCTCGCCATGCAGGTGAAGCTGTTGCGCGTGCTGCAGGACCGCAAGATCGAACGGCTCGGCTCCAACACCGCCGTGC
CGGTGGACCTGCGCGTGATCGCCGCCACCAAGGAGGACCTTGCCGCCCTCGCCGACGCCGGCCGCTTCCGCAAGGATTTG
TGCTTCCGCCTCGACGTGGTGAAGCTGGTGCTGCCGCCGCTGCGCGAGCGGCGGGAGGACATTCCCCTGCTGTTCGAGCT
GTTCGTGGTGCAGGCGGCGGTGAAGTACCAGCGGCCGGTGACGGAAGTTCCCCCATCCCTGCGCCGTGCGCTCATGCTGG
CCGACTGGCCGGGCAACGTGCGCGAGCTGAAGAACGCCGCCGAGCGCCACATCCTGGGCTTCCTCGCCCCCGATTTCGCC
GCCGGCGGGGCCGCGACCCCCGGCCTCAACGAGTTGCTGGACCGGGTGGAGCGCCTCGTGATCGAGGATGCGCTCAAAGC
GTCGGGCCAGCGCATCTCGGCGGCGGCGCAGGCCCTCAACATCCCCCGCAAGACCCTCTCCGACCGCATGAAGCGGCTCG
GCCTCACCGCCGCGGAGTGA

Upstream 100 bases:

>100_bases
ACAGGCGGCGGCCTCGCCTTCGATCTCGTCATGGAGGCTGCGGAGAGCGCGGACGCCGCCCCGCCCCAGCCGGAAAGATT
GCGCCACGAAAGGACGCACC

Downstream 100 bases:

>100_bases
CCCTCGCGGTTCTCGGACAGTTTCGAGACTCTGCTTTCCCGTGCCTTCGGGCCCGGTATCCACTCCTGCCGCAAGCGTCC
CTCCCCTCCCGCCCTCGCGG

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MCAERALKSTPAPHPAARPRVVLVDDEEMIRITIEQTLELAGIAVETFDSVDAALPAIGRDFPGVVVSDVRMPVRDGLEL
LADVRRRDPELPVVLITGHGDVAMAVSAMREGAYDFIEKPFVSDAFVEVVRRALDKRALVMENRRLRMALDRGEAGDAIE
RCLVGQSPAIRRLRDDVAQLASTSADVLVLGETGAGKEQVARALHEGGSRRDKPFVAVNCGAIPESMFESEMFRHEAGAF
TGAGKKRIGKIEHASGGTLFLDEVESMPLAMQVKLLRVLQDRKIERLGSNTAVPVDLRVIAATKEDLAALADAGRFRKDL
CFRLDVVKLVLPPLRERREDIPLLFELFVVQAAVKYQRPVTEVPPSLRRALMLADWPGNVRELKNAAERHILGFLAPDFA
AGGAATPGLNELLDRVERLVIEDALKASGQRISAAAQALNIPRKTLSDRMKRLGLTAAE

Sequences:

>Translated_459_residues
MCAERALKSTPAPHPAARPRVVLVDDEEMIRITIEQTLELAGIAVETFDSVDAALPAIGRDFPGVVVSDVRMPVRDGLEL
LADVRRRDPELPVVLITGHGDVAMAVSAMREGAYDFIEKPFVSDAFVEVVRRALDKRALVMENRRLRMALDRGEAGDAIE
RCLVGQSPAIRRLRDDVAQLASTSADVLVLGETGAGKEQVARALHEGGSRRDKPFVAVNCGAIPESMFESEMFRHEAGAF
TGAGKKRIGKIEHASGGTLFLDEVESMPLAMQVKLLRVLQDRKIERLGSNTAVPVDLRVIAATKEDLAALADAGRFRKDL
CFRLDVVKLVLPPLRERREDIPLLFELFVVQAAVKYQRPVTEVPPSLRRALMLADWPGNVRELKNAAERHILGFLAPDFA
AGGAATPGLNELLDRVERLVIEDALKASGQRISAAAQALNIPRKTLSDRMKRLGLTAAE
>Mature_459_residues
MCAERALKSTPAPHPAARPRVVLVDDEEMIRITIEQTLELAGIAVETFDSVDAALPAIGRDFPGVVVSDVRMPVRDGLEL
LADVRRRDPELPVVLITGHGDVAMAVSAMREGAYDFIEKPFVSDAFVEVVRRALDKRALVMENRRLRMALDRGEAGDAIE
RCLVGQSPAIRRLRDDVAQLASTSADVLVLGETGAGKEQVARALHEGGSRRDKPFVAVNCGAIPESMFESEMFRHEAGAF
TGAGKKRIGKIEHASGGTLFLDEVESMPLAMQVKLLRVLQDRKIERLGSNTAVPVDLRVIAATKEDLAALADAGRFRKDL
CFRLDVVKLVLPPLRERREDIPLLFELFVVQAAVKYQRPVTEVPPSLRRALMLADWPGNVRELKNAAERHILGFLAPDFA
AGGAATPGLNELLDRVERLVIEDALKASGQRISAAAQALNIPRKTLSDRMKRLGLTAAE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=459, Percent_Identity=38.3442265795207, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1790299, Length=471, Percent_Identity=38.2165605095541, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1790437, Length=445, Percent_Identity=39.5505617977528, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1788905, Length=372, Percent_Identity=39.5161290322581, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI87082117, Length=322, Percent_Identity=43.7888198757764, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1789087, Length=346, Percent_Identity=41.6184971098266, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1789233, Length=227, Percent_Identity=44.9339207048458, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1786524, Length=321, Percent_Identity=41.1214953271028, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=39.2405063291139, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI87081872, Length=269, Percent_Identity=40.5204460966543, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1787583, Length=310, Percent_Identity=37.741935483871, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI87081858, Length=309, Percent_Identity=30.7443365695793, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789828, Length=283, Percent_Identity=32.1554770318021, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50168; Mature: 50168

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAERALKSTPAPHPAARPRVVLVDDEEMIRITIEQTLELAGIAVETFDSVDAALPAIGR
CCHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
DFPGVVVSDVRMPVRDGLELLADVRRRDPELPVVLITGHGDVAMAVSAMREGAYDFIEKP
CCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
FVSDAFVEVVRRALDKRALVMENRRLRMALDRGEAGDAIERCLVGQSPAIRRLRDDVAQL
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
ASTSADVLVLGETGAGKEQVARALHEGGSRRDKPFVAVNCGAIPESMFESEMFRHEAGAF
HCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
TGAGKKRIGKIEHASGGTLFLDEVESMPLAMQVKLLRVLQDRKIERLGSNTAVPVDLRVI
CCCCHHHHCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
AATKEDLAALADAGRFRKDLCFRLDVVKLVLPPLRERREDIPLLFELFVVQAAVKYQRPV
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCCC
TEVPPSLRRALMLADWPGNVRELKNAAERHILGFLAPDFAAGGAATPGLNELLDRVERLV
CCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
IEDALKASGQRISAAAQALNIPRKTLSDRMKRLGLTAAE
HHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCAERALKSTPAPHPAARPRVVLVDDEEMIRITIEQTLELAGIAVETFDSVDAALPAIGR
CCHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
DFPGVVVSDVRMPVRDGLELLADVRRRDPELPVVLITGHGDVAMAVSAMREGAYDFIEKP
CCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
FVSDAFVEVVRRALDKRALVMENRRLRMALDRGEAGDAIERCLVGQSPAIRRLRDDVAQL
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
ASTSADVLVLGETGAGKEQVARALHEGGSRRDKPFVAVNCGAIPESMFESEMFRHEAGAF
HCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
TGAGKKRIGKIEHASGGTLFLDEVESMPLAMQVKLLRVLQDRKIERLGSNTAVPVDLRVI
CCCCHHHHCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
AATKEDLAALADAGRFRKDLCFRLDVVKLVLPPLRERREDIPLLFELFVVQAAVKYQRPV
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCCC
TEVPPSLRRALMLADWPGNVRELKNAAERHILGFLAPDFAAGGAATPGLNELLDRVERLV
CCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
IEDALKASGQRISAAAQALNIPRKTLSDRMKRLGLTAAE
HHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]