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The map label for this gene is nadB [C]

Identifier: 154247856

GI number: 154247856

Start: 4352470

End: 4354104

Strand: Reverse

Name: nadB [C]

Synonym: Xaut_3933

Alternate gene names: 154247856

Gene position: 4354104-4352470 (Counterclockwise)

Preceding gene: 154247857

Following gene: 154247855

Centisome position: 82.01

GC content: 71.5

Gene sequence:

>1635_bases
ATGACCCACGCCACGACCTTCGCCATCCCCTACGCCGACGCCCTCGCTCGCCTCTCCCCTGGCGCCGATCCGGTGATGCG
GCCCGAGGCGCTATCGCGCGAGCAGTTGCTTGAGGCCTACCACCCCGACCATGGCGCGGACGCCCGCGTGCCGCTGCGGG
TGGGCGCCAATCGCGGTGAACCGTGCCATCCCGCCCTTGCCCGCATCATCGAGGGCAATGCGCTCATCGGCGACGTGGAC
CTGGTGGCCGTCGATCCCATCGCCACCGACGTGCTGGTGATCGGCGGTGGCGGCGCCGGCACGGCCGCGACGCTGGCGGC
CCGCGCGGCCGGTGCCGAGGTCATCCTCGCCGCCAAGCTGCGCATCGGCGACAGCAACACGGTGATGGCCGAGGGCGGCA
TCCAGGCCGCCATCGGCGAGGACGACAGCCTCCGCCGCCATTTCGACGACACCATGAAGGGCGGCCACGGCGCCGGCTTT
CCCCAGCTGGTGGCCCAGCTTGTCAGCGACGCGCCGGACGTGATCCGCTGGCTTATCGGCCTCGGCGTCAATTTCGATCT
GGCGGACGGACCCGCCCCCGGCGCGCGGCTGATGCGCAAGCGGGCCGGCGGCACCACGGCGGCGCGCATTTTGAGCCATC
GCGATTTCACCGGTCTCGAAATGATGCGGGTGCTGCGCGAGGCGGTGGAGCTGGATCGCGGCGTCACCGTGCTCAATCGC
TCCCCCGTGGTGGAGTTGCTCTCCGACGAACGCGGCCGTTGCGCCGGCGCCGTGCTCTATGACCTGGAGCACCGCGCCTT
CCGCGTGGTGCGCGCCGGCGCAGTGGTGCTGGCAACTGGCGGCGCCGGGCGGCTGCACCTCACCGGCTTCGCCACCTCCA
ACCATTACGGCGCCACGGCGGACGGCCTCGCCCTCGCCTATCGCATGGGCGCCCGGCTGCGCGACGTGGATTCCTTCCAG
TATCACCCCACCGGCATCGCCTGGCCGCCGCATCTGGCCGGCGGGCTCATCTCGGAGGCGGTGCGCTCGTTGGGCGCAAA
GCTGGTGAACGGGCTCGGCGAGCGCTTCATCGACGAATTAGCCCCGCGCGACGTGGTCACCGCCGCCATCCTGCGCGAAT
GCGCCGAGGGGCGCGGGGTATCGCGCGACGGCGGGCTCGGCGTGTTCCTCGATACCCCGGCGCTGCTGGCCGAAAAGCCC
GACCTGCTGACGACACAGCTCATCTCGCTGGCCCATCTCGCCCATCGCGCCGGGGTGGACCCGGCCCGCGAGCCGCTGAT
GATCTATCCCACCCTGCACTATCAGAACGGTGGCGTGGCCATCGACCGCGACGGCACCTCCAGCGTGCCGGGCCTTTATT
GCGCAGGCGAGGTGACGGGCGGCATCCATGGCCGCAACCGGCTGATGGGCAATGCCCTGCTGGACATCTTCGTGTTCGGC
CGGCGGGCCGGCACGGCGGCGGCGGACCATGCGGGCGGGGTCCTGCCCGGCCGCTTCGGCCTCGAGCATGTGCAGCGCTG
GCAGCGCGAGCTCAGCCACGCCGGCATGATCGACGGTCCGCGCTCGCCGATCCTCTATCCCGCCTACGGCCATTTCGACC
TGCGCGGCCACATGGCCGGCGCCAACCAGGGCTGA

Upstream 100 bases:

>100_bases
TCATTCGCCGCCTTAACGAACTGGAGCTGGGCGAGGCCTGCCTCGACCTGACCGGGACCGAAGCCGAGCCTGCCTCCACC
CCGGCCCGGAGGTGAATTCG

Downstream 100 bases:

>100_bases
CCGCCATGACACGCCCCAACGTCCCCTCGAAGGCCAACACCGTCCTGCTCGATCCCGCCCTGAAGCTGGGCGCCAACCTG
CCGCTCTGGCGCCGCGCCGG

Product: L-aspartate oxidase

Products: oxaloacetate; NH3; H2O2; iminoaspartate [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTHATTFAIPYADALARLSPGADPVMRPEALSREQLLEAYHPDHGADARVPLRVGANRGEPCHPALARIIEGNALIGDVD
LVAVDPIATDVLVIGGGGAGTAATLAARAAGAEVILAAKLRIGDSNTVMAEGGIQAAIGEDDSLRRHFDDTMKGGHGAGF
PQLVAQLVSDAPDVIRWLIGLGVNFDLADGPAPGARLMRKRAGGTTAARILSHRDFTGLEMMRVLREAVELDRGVTVLNR
SPVVELLSDERGRCAGAVLYDLEHRAFRVVRAGAVVLATGGAGRLHLTGFATSNHYGATADGLALAYRMGARLRDVDSFQ
YHPTGIAWPPHLAGGLISEAVRSLGAKLVNGLGERFIDELAPRDVVTAAILRECAEGRGVSRDGGLGVFLDTPALLAEKP
DLLTTQLISLAHLAHRAGVDPAREPLMIYPTLHYQNGGVAIDRDGTSSVPGLYCAGEVTGGIHGRNRLMGNALLDIFVFG
RRAGTAAADHAGGVLPGRFGLEHVQRWQRELSHAGMIDGPRSPILYPAYGHFDLRGHMAGANQG

Sequences:

>Translated_544_residues
MTHATTFAIPYADALARLSPGADPVMRPEALSREQLLEAYHPDHGADARVPLRVGANRGEPCHPALARIIEGNALIGDVD
LVAVDPIATDVLVIGGGGAGTAATLAARAAGAEVILAAKLRIGDSNTVMAEGGIQAAIGEDDSLRRHFDDTMKGGHGAGF
PQLVAQLVSDAPDVIRWLIGLGVNFDLADGPAPGARLMRKRAGGTTAARILSHRDFTGLEMMRVLREAVELDRGVTVLNR
SPVVELLSDERGRCAGAVLYDLEHRAFRVVRAGAVVLATGGAGRLHLTGFATSNHYGATADGLALAYRMGARLRDVDSFQ
YHPTGIAWPPHLAGGLISEAVRSLGAKLVNGLGERFIDELAPRDVVTAAILRECAEGRGVSRDGGLGVFLDTPALLAEKP
DLLTTQLISLAHLAHRAGVDPAREPLMIYPTLHYQNGGVAIDRDGTSSVPGLYCAGEVTGGIHGRNRLMGNALLDIFVFG
RRAGTAAADHAGGVLPGRFGLEHVQRWQRELSHAGMIDGPRSPILYPAYGHFDLRGHMAGANQG
>Mature_543_residues
THATTFAIPYADALARLSPGADPVMRPEALSREQLLEAYHPDHGADARVPLRVGANRGEPCHPALARIIEGNALIGDVDL
VAVDPIATDVLVIGGGGAGTAATLAARAAGAEVILAAKLRIGDSNTVMAEGGIQAAIGEDDSLRRHFDDTMKGGHGAGFP
QLVAQLVSDAPDVIRWLIGLGVNFDLADGPAPGARLMRKRAGGTTAARILSHRDFTGLEMMRVLREAVELDRGVTVLNRS
PVVELLSDERGRCAGAVLYDLEHRAFRVVRAGAVVLATGGAGRLHLTGFATSNHYGATADGLALAYRMGARLRDVDSFQY
HPTGIAWPPHLAGGLISEAVRSLGAKLVNGLGERFIDELAPRDVVTAAILRECAEGRGVSRDGGLGVFLDTPALLAEKPD
LLTTQLISLAHLAHRAGVDPAREPLMIYPTLHYQNGGVAIDRDGTSSVPGLYCAGEVTGGIHGRNRLMGNALLDIFVFGR
RAGTAAADHAGGVLPGRFGLEHVQRWQRELSHAGMIDGPRSPILYPAYGHFDLRGHMAGANQG

Specific function: Catalyzes The Oxidation Of L-Aspartate To Iminoaspartate. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=397, Percent_Identity=34.7607052896725, Blast_Score=168, Evalue=9e-42,
Organism=Escherichia coli, GI1788928, Length=412, Percent_Identity=35.4368932038835, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1786942, Length=419, Percent_Identity=34.6062052505967, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1790597, Length=399, Percent_Identity=32.3308270676692, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17505833, Length=401, Percent_Identity=34.4139650872818, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17550100, Length=401, Percent_Identity=34.4139650872818, Blast_Score=164, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71986328, Length=453, Percent_Identity=27.3730684326711, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322701, Length=436, Percent_Identity=33.0275229357798, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322416, Length=439, Percent_Identity=31.8906605922551, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320788, Length=477, Percent_Identity=29.979035639413, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6322511, Length=476, Percent_Identity=27.9411764705882, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137288, Length=432, Percent_Identity=35.1851851851852, Blast_Score=171, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24655642, Length=432, Percent_Identity=35.1851851851852, Blast_Score=171, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24655647, Length=432, Percent_Identity=35.1851851851852, Blast_Score=171, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24663005, Length=444, Percent_Identity=31.0810810810811, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.4.3.16 [C]

Molecular weight: Translated: 57223; Mature: 57092

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHATTFAIPYADALARLSPGADPVMRPEALSREQLLEAYHPDHGADARVPLRVGANRGE
CCCCEEEECCHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHCCCCCCCCEEEEEECCCCCC
PCHPALARIIEGNALIGDVDLVAVDPIATDVLVIGGGGAGTAATLAARAAGAEVILAAKL
CCHHHHHHHHCCCEEEECEEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEE
RIGDSNTVMAEGGIQAAIGEDDSLRRHFDDTMKGGHGAGFPQLVAQLVSDAPDVIRWLIG
EECCCCEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHH
LGVNFDLADGPAPGARLMRKRAGGTTAARILSHRDFTGLEMMRVLREAVELDRGVTVLNR
CCCCEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEECC
SPVVELLSDERGRCAGAVLYDLEHRAFRVVRAGAVVLATGGAGRLHLTGFATSNHYGATA
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCCCCCCCH
DGLALAYRMGARLRDVDSFQYHPTGIAWPPHLAGGLISEAVRSLGAKLVNGLGERFIDEL
HHHHHHHHHCCHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APRDVVTAAILRECAEGRGVSRDGGLGVFLDTPALLAEKPDLLTTQLISLAHLAHRAGVD
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCC
PAREPLMIYPTLHYQNGGVAIDRDGTSSVPGLYCAGEVTGGIHGRNRLMGNALLDIFVFG
CCCCCEEEEEEEEECCCCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHC
RRAGTAAADHAGGVLPGRFGLEHVQRWQRELSHAGMIDGPRSPILYPAYGHFDLRGHMAG
CCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCC
ANQG
CCCC
>Mature Secondary Structure 
THATTFAIPYADALARLSPGADPVMRPEALSREQLLEAYHPDHGADARVPLRVGANRGE
CCCEEEECCHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHCCCCCCCCEEEEEECCCCCC
PCHPALARIIEGNALIGDVDLVAVDPIATDVLVIGGGGAGTAATLAARAAGAEVILAAKL
CCHHHHHHHHCCCEEEECEEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEE
RIGDSNTVMAEGGIQAAIGEDDSLRRHFDDTMKGGHGAGFPQLVAQLVSDAPDVIRWLIG
EECCCCEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHH
LGVNFDLADGPAPGARLMRKRAGGTTAARILSHRDFTGLEMMRVLREAVELDRGVTVLNR
CCCCEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEECC
SPVVELLSDERGRCAGAVLYDLEHRAFRVVRAGAVVLATGGAGRLHLTGFATSNHYGATA
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCCCCCCCH
DGLALAYRMGARLRDVDSFQYHPTGIAWPPHLAGGLISEAVRSLGAKLVNGLGERFIDEL
HHHHHHHHHCCHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APRDVVTAAILRECAEGRGVSRDGGLGVFLDTPALLAEKPDLLTTQLISLAHLAHRAGVD
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCC
PAREPLMIYPTLHYQNGGVAIDRDGTSSVPGLYCAGEVTGGIHGRNRLMGNALLDIFVFG
CCCCCEEEEEEEEECCCCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHC
RRAGTAAADHAGGVLPGRFGLEHVQRWQRELSHAGMIDGPRSPILYPAYGHFDLRGHMAG
CCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCC
ANQG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: L-Aspartate [C]

Kcat value (1/min): 2 [C]

Specific activity: NA

Km value (mM): 4.1 {L-aspartate}} 0.5 {L-aspartate}} [C]

Substrates: @ASPA02txt*L-Aspartate!; H2O; O2; L-aspartate [C]

Specific reaction: @ASOA02.txt*L-Aspartate! + H2O + O2 = oxaloacetate + NH3 + H2O2 O2 + L-aspartate = H2O2 + iminoaspartate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: Iodoacetate; L-Aspartate; Mesotartrate; NAD+; Tetranitromethane; Urea [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]