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The map label for this gene is nuoF2 [H]

Identifier: 154247855

GI number: 154247855

Start: 4351196

End: 4352464

Strand: Reverse

Name: nuoF2 [H]

Synonym: Xaut_3932

Alternate gene names: 154247855

Gene position: 4352464-4351196 (Counterclockwise)

Preceding gene: 154247856

Following gene: 154247853

Centisome position: 81.98

GC content: 69.19

Gene sequence:

>1269_bases
ATGACACGCCCCAACGTCCCCTCGAAGGCCAACACCGTCCTGCTCGATCCCGCCCTGAAGCTGGGCGCCAACCTGCCGCT
CTGGCGCCGCGCCGGCGGCGGGGCCGGCCTTGCCGCCGCCCTCGCCCGGCCCGACGCCATCATTGAGACCCTCAAGGACG
CCGACCTGCGCGGCATGGGCGGCGCGGGCTTCCCCACCTGGCGCAAGTGGAGCGCCGCCGCGGCCTCGCCCTGCGACGAG
AAATATGTGGTCTGCAACGGCAATGAGGACGAGCCCGGCACCTTCAAGGACCGCCATCTGCTGCGCTGGACGCCCCACCA
GGTGATCGAGGGCGCGCTGATCGCCGCCGTCGCGGTGAAGGCCAATCGCGTGGTGTTCTACGTCAATCCCCACCAGGCAG
AGGGCATCGACCAGATGCGCTGGGCGGTGGACGAATGGACCGCCAGCGACCTCCTCGCCTCCGTCTCCAAGGTGGTGGGC
CGGCCTGTGACGCTCACGGTGGCGCCCAGCTCGGGCCGCTATATCGGTGGCGAGGAGACGGCCATCGTGTCCTGGCTCGA
CGGCGGCTTCCCCTTCCCGCGCCGCAAGCCGCCATTCCCGTTCGAGAGCGGGGTGGGCGGCCTGCCGACGCTGATCAACA
ACACCGAGACCCTCGCCAACGTGCCGCACATCCTGAAGAACGGCGCCGACTGGTATCGCGACCTCGGCGTGGGCGCGGCG
GCGGGCACCAAGCTGTTTTCGCTGTCGGGCGACGTGCTCAATCCCGGCCTCTACGAGCTGCCCATGGGCACCAGCCTTGA
GGAACTTGTCTTCGTCCATGGCGGCGGCATGCTGGATGGTCGCGCCTTCAAGGCGGTGTTCACCGGCGGGCCATCCAACA
CGCTGCTCACGGCACGCGACCTCGACGTGGCCCTCGACTTCGACAGTGTGAAGAGGCGCAAGTCGCGGCTCGGCACCGGC
GCCATGATCGTCATCTCGGAAGGCACCAGCATCGTGCGCAAGGTGGCGGAATATGTCGCCTTCTTCGCCAGCGGCTCGTG
CGGGCAATGCCCGTCCTGCAAGTGCGGCACCTTCCAGATGGCCCGCCTCTTGGACCGCATCGCCACCGGCCGGGGCGTGG
AAGCCGACCGGCAGGCACTGGACCATCTGTGCCGCATTCTGCCCGGCAGCGGGCGCTGCGCGCTCATCGACGGCGCTGTG
ACGGTGGTGGAAAGCTCGCGCCACACCTTCCCGGACGAGTACGAGCCCAAGGCGCGCTACGCGTCCTGA

Upstream 100 bases:

>100_bases
CCGGCATGATCGACGGTCCGCGCTCGCCGATCCTCTATCCCGCCTACGGCCATTTCGACCTGCGCGGCCACATGGCCGGC
GCCAACCAGGGCTGACCGCC

Downstream 100 bases:

>100_bases
CGGTCGCCTGCGGCCAGGCGGCGGTGCCAGGCGGCGGCCGCTTCGCCGGCGAGCATCCGCAGTTCGCGCAGCGCCGCCGC
ATCCTCGTGCGACAGGCGCT

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTRPNVPSKANTVLLDPALKLGANLPLWRRAGGGAGLAAALARPDAIIETLKDADLRGMGGAGFPTWRKWSAAAASPCDE
KYVVCNGNEDEPGTFKDRHLLRWTPHQVIEGALIAAVAVKANRVVFYVNPHQAEGIDQMRWAVDEWTASDLLASVSKVVG
RPVTLTVAPSSGRYIGGEETAIVSWLDGGFPFPRRKPPFPFESGVGGLPTLINNTETLANVPHILKNGADWYRDLGVGAA
AGTKLFSLSGDVLNPGLYELPMGTSLEELVFVHGGGMLDGRAFKAVFTGGPSNTLLTARDLDVALDFDSVKRRKSRLGTG
AMIVISEGTSIVRKVAEYVAFFASGSCGQCPSCKCGTFQMARLLDRIATGRGVEADRQALDHLCRILPGSGRCALIDGAV
TVVESSRHTFPDEYEPKARYAS

Sequences:

>Translated_422_residues
MTRPNVPSKANTVLLDPALKLGANLPLWRRAGGGAGLAAALARPDAIIETLKDADLRGMGGAGFPTWRKWSAAAASPCDE
KYVVCNGNEDEPGTFKDRHLLRWTPHQVIEGALIAAVAVKANRVVFYVNPHQAEGIDQMRWAVDEWTASDLLASVSKVVG
RPVTLTVAPSSGRYIGGEETAIVSWLDGGFPFPRRKPPFPFESGVGGLPTLINNTETLANVPHILKNGADWYRDLGVGAA
AGTKLFSLSGDVLNPGLYELPMGTSLEELVFVHGGGMLDGRAFKAVFTGGPSNTLLTARDLDVALDFDSVKRRKSRLGTG
AMIVISEGTSIVRKVAEYVAFFASGSCGQCPSCKCGTFQMARLLDRIATGRGVEADRQALDHLCRILPGSGRCALIDGAV
TVVESSRHTFPDEYEPKARYAS
>Mature_421_residues
TRPNVPSKANTVLLDPALKLGANLPLWRRAGGGAGLAAALARPDAIIETLKDADLRGMGGAGFPTWRKWSAAAASPCDEK
YVVCNGNEDEPGTFKDRHLLRWTPHQVIEGALIAAVAVKANRVVFYVNPHQAEGIDQMRWAVDEWTASDLLASVSKVVGR
PVTLTVAPSSGRYIGGEETAIVSWLDGGFPFPRRKPPFPFESGVGGLPTLINNTETLANVPHILKNGADWYRDLGVGAAA
GTKLFSLSGDVLNPGLYELPMGTSLEELVFVHGGGMLDGRAFKAVFTGGPSNTLLTARDLDVALDFDSVKRRKSRLGTGA
MIVISEGTSIVRKVAEYVAFFASGSCGQCPSCKCGTFQMARLLDRIATGRGVEADRQALDHLCRILPGSGRCALIDGAVT
VVESSRHTFPDEYEPKARYAS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=385, Percent_Identity=36.6233766233766, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI260656005, Length=385, Percent_Identity=36.6233766233766, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1788620, Length=401, Percent_Identity=41.645885286783, Blast_Score=284, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI17531727, Length=381, Percent_Identity=36.2204724409449, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI45550452, Length=356, Percent_Identity=36.7977528089888, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI19920794, Length=379, Percent_Identity=35.6200527704486, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24653819, Length=389, Percent_Identity=33.9331619537275, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24653817, Length=370, Percent_Identity=33.2432432432432, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 45049; Mature: 44918

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPNVPSKANTVLLDPALKLGANLPLWRRAGGGAGLAAALARPDAIIETLKDADLRGMG
CCCCCCCCCCCEEEECCHHHHCCCCCCEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCCC
GAGFPTWRKWSAAAASPCDEKYVVCNGNEDEPGTFKDRHLLRWTPHQVIEGALIAAVAVK
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHEEE
ANRVVFYVNPHQAEGIDQMRWAVDEWTASDLLASVSKVVGRPVTLTVAPSSGRYIGGEET
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCE
AIVSWLDGGFPFPRRKPPFPFESGVGGLPTLINNTETLANVPHILKNGADWYRDLGVGAA
EEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHCCCCC
AGTKLFSLSGDVLNPGLYELPMGTSLEELVFVHGGGMLDGRAFKAVFTGGPSNTLLTARD
CCCEEEEECCCCCCCCCEECCCCCCHHHEEEEECCCEECCCEEEEEEECCCCCCEEEEEC
LDVALDFDSVKRRKSRLGTGAMIVISEGTSIVRKVAEYVAFFASGSCGQCPSCKCGTFQM
CEEEECHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
ARLLDRIATGRGVEADRQALDHLCRILPGSGRCALIDGAVTVVESSRHTFPDEYEPKARY
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECEEEEEECCCCCCCCCCCCCCCC
AS
CC
>Mature Secondary Structure 
TRPNVPSKANTVLLDPALKLGANLPLWRRAGGGAGLAAALARPDAIIETLKDADLRGMG
CCCCCCCCCCEEEECCHHHHCCCCCCEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCCC
GAGFPTWRKWSAAAASPCDEKYVVCNGNEDEPGTFKDRHLLRWTPHQVIEGALIAAVAVK
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHEEE
ANRVVFYVNPHQAEGIDQMRWAVDEWTASDLLASVSKVVGRPVTLTVAPSSGRYIGGEET
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCE
AIVSWLDGGFPFPRRKPPFPFESGVGGLPTLINNTETLANVPHILKNGADWYRDLGVGAA
EEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHCCCCC
AGTKLFSLSGDVLNPGLYELPMGTSLEELVFVHGGGMLDGRAFKAVFTGGPSNTLLTARD
CCCEEEEECCCCCCCCCEECCCCCCHHHEEEEECCCEECCCEEEEEEECCCCCCEEEEEC
LDVALDFDSVKRRKSRLGTGAMIVISEGTSIVRKVAEYVAFFASGSCGQCPSCKCGTFQM
CEEEECHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
ARLLDRIATGRGVEADRQALDHLCRILPGSGRCALIDGAVTVVESSRHTFPDEYEPKARY
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECEEEEEECCCCCCCCCCCCCCCC
AS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]