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The map label for this gene is cobD [H]

Identifier: 154247703

GI number: 154247703

Start: 4186091

End: 4187062

Strand: Reverse

Name: cobD [H]

Synonym: Xaut_3779

Alternate gene names: 154247703

Gene position: 4187062-4186091 (Counterclockwise)

Preceding gene: 154247708

Following gene: 154247691

Centisome position: 78.87

GC content: 72.22

Gene sequence:

>972_bases
ATGGCATTCGATCTGACGGCGCTGGCCGTGCTCCTGGAAGCCCTGCTCGGCTATCCGGCGGGCTTGGTGCGCCGCATTGG
CCATCCGGTGATCTGGATCGGCCGGCTGATCGTCGTGCTGGACCACGGCCTCAACCGCCCCGGCTTGACCGCCACGACGC
GCAAGGGCCTCGGCGTGGTGGCGGTGGGAGTGATCCTTGTCGCGGCCGCCGTTCCCGCCGCGCTTCTTGAAGCCCTGCTG
CGTCCCCTGCCCTTCGGCCTGCTGGTGCTGGGCCTGATCGGCTCGACGCTCATCGCCCAGCGCTCGCTGCACTGGCATGT
GGCGCGGGTGGCCGAGGCGCTGGAAGGCGGCAGTCTGGAGGCGGCACGCACCGCCGTGAGCCATATCGTCGGGCGCGACC
CGCAGACCTTGGATGAGGCCGGCGTCGCCCGCGCCGCCATCGAGAGCCTCGCCGAGAATTTCTCCGACGGCGTGGTTGCT
CCCACACTGTGGATGTGTGTGGGCGGGCTGGCCGGGGGCGCCGCCTACAAGGCGCTCAACACCGCCGACAGCATGATCGG
CCATCTCACCGAGCGCCACGCCGCCTTCGGCTGGGCCGCCGCCCGCACCGACGATTATGTGAACCTGCCCGCCTCGCGCC
TCGCCGGCATGCTGCTGATCGCGGCCGCCGCCCTCACCCCCGGCACCGATGCCCGCGCCGCCCACACGGCCATGCTGCGG
GATGCGCCGCGGCACCGTTCCCCCAATGCCGGCTGGTCGGAGGCGGCCATGGCCGGCGCGCTGGGGCTGAAGCTGAACGG
CCCCAAGGTCTATGCCGGCGTCTTCACCGAGGACGCCTACATGGGCGACGGGCGACGGGAGGCAACGGCGAAGGACGTGC
GCGCGGCGCTGCGGCTCTACCGCCGGGCGGATGCGCTGATGGTGCTGCTGGTGCTCGCGGGCGCCGCGGCCCCCTTCATC
TGGCGAGGCTGA

Upstream 100 bases:

>100_bases
ACGCGCGTCAAAACGCTCCACACCAAGCACTTGAAACAGCAGGCGCTTGCGGCGTTGGCACAAGACAGGCACAGGGCACC
GTGGCCGCGGGAGAGGCGTG

Downstream 100 bases:

>100_bases
ACAGCAGGTCGAGGTCGAGATGGGCGGCGAGGTGCGCGGCGAGGCGATCCAGCGTCTCCTCCACACCCTGCTCATAGGCC
GCCTGCGACGGGGCCCCGCC

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAFDLTALAVLLEALLGYPAGLVRRIGHPVIWIGRLIVVLDHGLNRPGLTATTRKGLGVVAVGVILVAAAVPAALLEALL
RPLPFGLLVLGLIGSTLIAQRSLHWHVARVAEALEGGSLEAARTAVSHIVGRDPQTLDEAGVARAAIESLAENFSDGVVA
PTLWMCVGGLAGGAAYKALNTADSMIGHLTERHAAFGWAAARTDDYVNLPASRLAGMLLIAAAALTPGTDARAAHTAMLR
DAPRHRSPNAGWSEAAMAGALGLKLNGPKVYAGVFTEDAYMGDGRREATAKDVRAALRLYRRADALMVLLVLAGAAAPFI
WRG

Sequences:

>Translated_323_residues
MAFDLTALAVLLEALLGYPAGLVRRIGHPVIWIGRLIVVLDHGLNRPGLTATTRKGLGVVAVGVILVAAAVPAALLEALL
RPLPFGLLVLGLIGSTLIAQRSLHWHVARVAEALEGGSLEAARTAVSHIVGRDPQTLDEAGVARAAIESLAENFSDGVVA
PTLWMCVGGLAGGAAYKALNTADSMIGHLTERHAAFGWAAARTDDYVNLPASRLAGMLLIAAAALTPGTDARAAHTAMLR
DAPRHRSPNAGWSEAAMAGALGLKLNGPKVYAGVFTEDAYMGDGRREATAKDVRAALRLYRRADALMVLLVLAGAAAPFI
WRG
>Mature_322_residues
AFDLTALAVLLEALLGYPAGLVRRIGHPVIWIGRLIVVLDHGLNRPGLTATTRKGLGVVAVGVILVAAAVPAALLEALLR
PLPFGLLVLGLIGSTLIAQRSLHWHVARVAEALEGGSLEAARTAVSHIVGRDPQTLDEAGVARAAIESLAENFSDGVVAP
TLWMCVGGLAGGAAYKALNTADSMIGHLTERHAAFGWAAARTDDYVNLPASRLAGMLLIAAAALTPGTDARAAHTAMLRD
APRHRSPNAGWSEAAMAGALGLKLNGPKVYAGVFTEDAYMGDGRREATAKDVRAALRLYRRADALMVLLVLAGAAAPFIW
RG

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 33623; Mature: 33491

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFDLTALAVLLEALLGYPAGLVRRIGHPVIWIGRLIVVLDHGLNRPGLTATTRKGLGVV
CCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHH
AVGVILVAAAVPAALLEALLRPLPFGLLVLGLIGSTLIAQRSLHWHVARVAEALEGGSLE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AARTAVSHIVGRDPQTLDEAGVARAAIESLAENFSDGVVAPTLWMCVGGLAGGAAYKALN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
TADSMIGHLTERHAAFGWAAARTDDYVNLPASRLAGMLLIAAAALTPGTDARAAHTAMLR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DAPRHRSPNAGWSEAAMAGALGLKLNGPKVYAGVFTEDAYMGDGRREATAKDVRAALRLY
CCCCCCCCCCCHHHHHHHHHHCEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
RRADALMVLLVLAGAAAPFIWRG
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AFDLTALAVLLEALLGYPAGLVRRIGHPVIWIGRLIVVLDHGLNRPGLTATTRKGLGVV
CCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHH
AVGVILVAAAVPAALLEALLRPLPFGLLVLGLIGSTLIAQRSLHWHVARVAEALEGGSLE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AARTAVSHIVGRDPQTLDEAGVARAAIESLAENFSDGVVAPTLWMCVGGLAGGAAYKALN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
TADSMIGHLTERHAAFGWAAARTDDYVNLPASRLAGMLLIAAAALTPGTDARAAHTAMLR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DAPRHRSPNAGWSEAAMAGALGLKLNGPKVYAGVFTEDAYMGDGRREATAKDVRAALRLY
CCCCCCCCCCCHHHHHHHHHHCEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
RRADALMVLLVLAGAAAPFIWRG
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2211520 [H]