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The map label for this gene is glnA [H]

Identifier: 154247512

GI number: 154247512

Start: 4007265

End: 4008680

Strand: Reverse

Name: glnA [H]

Synonym: Xaut_3585

Alternate gene names: 154247512

Gene position: 4008680-4007265 (Counterclockwise)

Preceding gene: 154247513

Following gene: 154247509

Centisome position: 75.51

GC content: 63.35

Gene sequence:

>1416_bases
ATGACCATCAAGACGGCCAAGGACGTCCTCGACCTGATCAAGAGCGAGGACGTGAAATACGTCGATCTGCGCTTCACCGA
CCCGCGCGGAAAATGGCAGCATGTTACCTTCGACATTTCCATGGTCGACGATGAGTTCCTGACCGAAGGCACCGCCTTCG
ACGGCTCGTCCATCGCCGGCTGGAAGGCCATCAACGAATCCGACATGCTGCTGATGCCGGATCCGGCCACCACCTGCATT
GACCCGTTCTTCGCCGAGACCACGCTCTCCATCGTCTGCGACGTGCTGGAGCCGACCACCAACCAGCCCTACGGCCGCGA
CCCCCGCTCCATCGCCCGCAAGGCCGAGGCCTATGCGAAGTCCACCGGCATCGGCGATGCCGTCTATTTCGGCCCCGAGG
CCGAGTTCTTCATCTTCGACGACGTGCGCTTCAAGGCGGACCCGTACAACACGGGCTTCAAGCTCGACTCGGTCGAGCTG
CCCACCAACGGCGACACCGAATATGAGGGTGGCAACCTCGGCCACCGGGTGAAGACCAAGGGCGGCTACTTCCCCGTGCC
GCCGATCGATTCGGCGCAGGACATGCGCTCCGAGATGCTCGCCGCCATGGCCAAGATGGGCGCCAAGGTGGAGAAGCACC
ACCACGAGGTGGCCTCCGCCCAGCACGAGCTCGGCCTCAAGTTCGGCTCGCTGGTGACCATGGCCGACCACCTGCAGGTC
TATAAGTACTGCATCCACCAGGTGGCCAACATCTACGGCAAGACCGCGACCTTCATGCCGAAGCCCGTCTTCGGCGACAA
CGGCTCGGGCATGCACGTCCACCAGTCCATCTGGAAGGACGGCAAGCCGCTCTTCGCCGGCGACAAGTATGCGGACCTGT
CCGACTACTGCCTGTGGTACATCGGCGGCATCATCAAGCATGCCAAGTCGCTGAACGCCTTCACCAACCCGCTGACCAAC
TCCTACAAGCGCCTGGTCCCGGGCTATGAGGCTCCGGTGCTGCTGGCCTATTCGGCCCGCAACCGCTCGGCGTCCTGCCG
CATCCCCTACACCAACAACCCGAAGGCCAAGCGCGTCGAGGTCCGCTTCCCCGATCCCGGCGCGAACCCCTACCTCGCCT
TCGCGGCCCTGTTCATGGCCGGCATGGACGGCATCCTCAACCGGATCGACCCCGGCCAGGCCATGGACAAGGACCTGTAC
GACCTGCCCCCCGCTGAGCTGAAGCAGATCCCCACGGTCTGCGGCTCGCTGCGCGAGGCCCTGGATTCGCTCGCCGCCGA
CCACGAGTATCTGCTCAAGGGCGACGTGTTCCAGAAGGACTTCATCGAGTCCTACATCGAGCTGAAGATGACCGAAGTGA
TGCGTTTCGAAATGACGCCGCACCCGGTCGAGTTCGAGATGTACTACTCGGTCTGA

Upstream 100 bases:

>100_bases
CATCGAGGAGGCGATCCGCATCCGCACCGGCGAATCCGGCCTCGACGCGATTTGACGCGTCCAATAAAAACCTGCGTGCC
AGCAGAAAGGTAATACCCCC

Downstream 100 bases:

>100_bases
CGAACATCTCCGGCCCGGCCAAAAGGCTGGGCCGGCCTTGTTCCGGTCTTCATGACCTCATTGGGCGCCTCTCGGGGCGC
CCTTTCTTTTAGCAGAGGTC

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTIKTAKDVLDLIKSEDVKYVDLRFTDPRGKWQHVTFDISMVDDEFLTEGTAFDGSSIAGWKAINESDMLLMPDPATTCI
DPFFAETTLSIVCDVLEPTTNQPYGRDPRSIARKAEAYAKSTGIGDAVYFGPEAEFFIFDDVRFKADPYNTGFKLDSVEL
PTNGDTEYEGGNLGHRVKTKGGYFPVPPIDSAQDMRSEMLAAMAKMGAKVEKHHHEVASAQHELGLKFGSLVTMADHLQV
YKYCIHQVANIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPLFAGDKYADLSDYCLWYIGGIIKHAKSLNAFTNPLTN
SYKRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVEVRFPDPGANPYLAFAALFMAGMDGILNRIDPGQAMDKDLY
DLPPAELKQIPTVCGSLREALDSLAADHEYLLKGDVFQKDFIESYIELKMTEVMRFEMTPHPVEFEMYYSV

Sequences:

>Translated_471_residues
MTIKTAKDVLDLIKSEDVKYVDLRFTDPRGKWQHVTFDISMVDDEFLTEGTAFDGSSIAGWKAINESDMLLMPDPATTCI
DPFFAETTLSIVCDVLEPTTNQPYGRDPRSIARKAEAYAKSTGIGDAVYFGPEAEFFIFDDVRFKADPYNTGFKLDSVEL
PTNGDTEYEGGNLGHRVKTKGGYFPVPPIDSAQDMRSEMLAAMAKMGAKVEKHHHEVASAQHELGLKFGSLVTMADHLQV
YKYCIHQVANIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPLFAGDKYADLSDYCLWYIGGIIKHAKSLNAFTNPLTN
SYKRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVEVRFPDPGANPYLAFAALFMAGMDGILNRIDPGQAMDKDLY
DLPPAELKQIPTVCGSLREALDSLAADHEYLLKGDVFQKDFIESYIELKMTEVMRFEMTPHPVEFEMYYSV
>Mature_470_residues
TIKTAKDVLDLIKSEDVKYVDLRFTDPRGKWQHVTFDISMVDDEFLTEGTAFDGSSIAGWKAINESDMLLMPDPATTCID
PFFAETTLSIVCDVLEPTTNQPYGRDPRSIARKAEAYAKSTGIGDAVYFGPEAEFFIFDDVRFKADPYNTGFKLDSVELP
TNGDTEYEGGNLGHRVKTKGGYFPVPPIDSAQDMRSEMLAAMAKMGAKVEKHHHEVASAQHELGLKFGSLVTMADHLQVY
KYCIHQVANIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPLFAGDKYADLSDYCLWYIGGIIKHAKSLNAFTNPLTNS
YKRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVEVRFPDPGANPYLAFAALFMAGMDGILNRIDPGQAMDKDLYD
LPPAELKQIPTVCGSLREALDSLAADHEYLLKGDVFQKDFIESYIELKMTEVMRFEMTPHPVEFEMYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=63.034188034188, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI87081870, Length=391, Percent_Identity=24.5524296675192, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52607; Mature: 52476

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKTAKDVLDLIKSEDVKYVDLRFTDPRGKWQHVTFDISMVDDEFLTEGTAFDGSSIAG
CCCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEECCHHHHHCCCCCCCCCCCC
WKAINESDMLLMPDPATTCIDPFFAETTLSIVCDVLEPTTNQPYGRDPRSIARKAEAYAK
CEECCCCCEEEECCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
STGIGDAVYFGPEAEFFIFDDVRFKADPYNTGFKLDSVELPTNGDTEYEGGNLGHRVKTK
HCCCCCEEEECCCCCEEEEECEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECC
GGYFPVPPIDSAQDMRSEMLAAMAKMGAKVEKHHHEVASAQHELGLKFGSLVTMADHLQV
CCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YKYCIHQVANIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPLFAGDKYADLSDYCLWY
HHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHH
IGGIIKHAKSLNAFTNPLTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEE
VRFPDPGANPYLAFAALFMAGMDGILNRIDPGQAMDKDLYDLPPAELKQIPTVCGSLREA
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHCCCHHHHHHHHHHHHHHHHH
LDSLAADHEYLLKGDVFQKDFIESYIELKMTEVMRFEMTPHPVEFEMYYSV
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TIKTAKDVLDLIKSEDVKYVDLRFTDPRGKWQHVTFDISMVDDEFLTEGTAFDGSSIAG
CCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEECCHHHHHCCCCCCCCCCCC
WKAINESDMLLMPDPATTCIDPFFAETTLSIVCDVLEPTTNQPYGRDPRSIARKAEAYAK
CEECCCCCEEEECCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
STGIGDAVYFGPEAEFFIFDDVRFKADPYNTGFKLDSVELPTNGDTEYEGGNLGHRVKTK
HCCCCCEEEECCCCCEEEEECEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECC
GGYFPVPPIDSAQDMRSEMLAAMAKMGAKVEKHHHEVASAQHELGLKFGSLVTMADHLQV
CCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YKYCIHQVANIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPLFAGDKYADLSDYCLWY
HHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHH
IGGIIKHAKSLNAFTNPLTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEE
VRFPDPGANPYLAFAALFMAGMDGILNRIDPGQAMDKDLYDLPPAELKQIPTVCGSLREA
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHCCCHHHHHHHHHHHHHHHHH
LDSLAADHEYLLKGDVFQKDFIESYIELKMTEVMRFEMTPHPVEFEMYYSV
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171403 [H]