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The map label for this gene is phbC [H]

Identifier: 154247451

GI number: 154247451

Start: 3928708

End: 3930648

Strand: Direct

Name: phbC [H]

Synonym: Xaut_3523

Alternate gene names: 154247451

Gene position: 3928708-3930648 (Clockwise)

Preceding gene: 154247447

Following gene: 154247452

Centisome position: 74.0

GC content: 66.31

Gene sequence:

>1941_bases
ATGCCTGCTCCGCACCTGACCGTGGTGCCTCCGGCACCGGCCGAGGCGCCCAAGCCGGCTGCCGGCAAGGCGTCGGAGAC
GAAGGTCGCGTCCGCAGAGGGCGCGTCCGCTGCCACCTCCAGGGCCCGTGCCCCGTCGCCCGCAGCCTCGCCCGCTGGCG
GCGACGGCGCGCGCAGCGGCATGGACGTGGAGGTTCTCGCCCGCAACCTCGCCCAGATGATGGAGGAGGGCGGCAAGGCC
ATGGCGGCCTACCTGGCCCCGCGCCATCCGGGCAAGACCGACGACATGGCCGAGGACATCGCCGACGCGCTCAAGACCGT
GGGCCACGTGGCCGAATACTGGATGGCCGATCCCCAGCGTACGGTGGAGGCGCAGTCGCGCCTGATGGCCGGCTATCTCT
CGGTCTGGGCCAACACCATGAAGCGCCTTGCGGGAGAGGAGGTGACGCCGGTCACCAAGCCCGACGCCAAGGACGCCCGC
TTCAAGGACCAGGGTTGGAGCGACAGCCCCATCTTCGACGCTCTCAAGCAGGCCTATCTCGTCACCGCCAAATGGGCCGA
GGACATGGTGGAGGAGGCCGAGGGCCTCGACCCGCACCTCAAGCACAAGGCCGAGTTCCTGGTACGCCAGGTGTCCAACG
CCATCTCGCCCTCGAACTTCGTGATGACCAATCCCGAGCTGATCCGCGAGACCATGAGCTCGTCGGGCGAGAACCTCGTC
AAGGGCATGCGGAACCTGACCGCCGACCTGATCGAGGGCAAGGGCACGCTCAAGATCCGCCAGACGGACATGAGCGCCTT
CGAGGTGGGCCGCAACCTCGCCACCACCCCGGGCAAGGTGATCTATGAGAATGAGCTGATGCAGCTCATCCAGTATGAGG
CCACGACAGCGAGCGTGAAGAAGACGCCGCTGTTGATCGTGCCGCCGTGGATCAACAAGTTCTATATTCTCGACCTGACG
GCCGAGAAGTCTTTCATCAAGTGGCTGGTGGACCAGGGCGTCAGCGTCTTCGTCATTTCCTGGGTCAATCCGGATGCCCG
GCTGGCCACCAAGGGCTTTGACGATTACATGCGCGAGGGCATCATGACCGCCCTCGACCAGGTGGCCATCGCCACCGGCG
AGCGGCAGGCCCACGCCATGGGCTATTGCGTGGGCGGCACCCTGCTCGCCACCACGCTCGCCTACATGGCCGCCACCGGG
GACGATCGCATCGCCTCCGCCACCTTCCTCACCACCCAGATCGACTTCACCCATGCGGGCGATCTCAAGGTCTTCGTGGA
CGAGGACCAGCTGGCCTCCATCGAGCGCAAGATGAAGGACATGGGGTATCTGGAGGGCAGCAAGATGGCCTCCGCCTTCA
ACCTGCTGCGCTCCAACGACCTGATCTGGCCCTATGTGGTGAACAACTACATGAAGGGCAAGGCGCCGTTCCCGTTCGAC
CTGTTCTTCTGGAACGCGGATTCCACCCGCATGCCGGCGGCGAACCACTCCTTCTACCTGCGCAACTGCTACCTCACCAA
CAACATCGCCCGCGGCCTTGCCGAACTCGGCGGCGTGCGCATCGACGTGCACAAGATCTCGGTGCCGGTCTATTCGCTCG
CCACCAAGGAAGACCATATCGCGCCGCCCAACTCGGTCTATATCGGCGCCAATCTCCTGTCCGGCCCGGTGCGCTACGTG
CTCGCCGGCTCCGGCCACATCGCTGGCGTGGTCAATCCGCCAGCCAAGGGCAAGTACCAGTACTGGGCGGACGGCCCGAC
TGGGCCGAGCTTCGACCTGTGGCTGGAAGGCGCTCGGGAGAGTGCCGGCTCCTGGTGGCCGGACTGGATCTCCTGGTTCA
GCGCCCAGCATCCCGACGAGGTCCCCGCCCGCACCATCGGCGGCGACCGCCTCACCCCCATCGAGGACGCGCCCGGCCGC
TACGTGCGGGCGCGCAGCTGA

Upstream 100 bases:

>100_bases
CTTTCCGTAACGGCCCGCTAAAAGGGAATGACCTATGTCCCGTGCAGAGGAAACGTCGAACATGGCAGGGAACGGCAAAG
GCATGGCTCCGCCCGTGGAG

Downstream 100 bases:

>100_bases
GGCAGCGTTTCGCGCGGACGGTGCGGGCAGGCCCGTGCCATCCGCGCGGACGCTTTTTCCAGTGAAGGTCAGGCTCGATC
CACACTTCGTGCGGCTCCGG

Product: poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MPAPHLTVVPPAPAEAPKPAAGKASETKVASAEGASAATSRARAPSPAASPAGGDGARSGMDVEVLARNLAQMMEEGGKA
MAAYLAPRHPGKTDDMAEDIADALKTVGHVAEYWMADPQRTVEAQSRLMAGYLSVWANTMKRLAGEEVTPVTKPDAKDAR
FKDQGWSDSPIFDALKQAYLVTAKWAEDMVEEAEGLDPHLKHKAEFLVRQVSNAISPSNFVMTNPELIRETMSSSGENLV
KGMRNLTADLIEGKGTLKIRQTDMSAFEVGRNLATTPGKVIYENELMQLIQYEATTASVKKTPLLIVPPWINKFYILDLT
AEKSFIKWLVDQGVSVFVISWVNPDARLATKGFDDYMREGIMTALDQVAIATGERQAHAMGYCVGGTLLATTLAYMAATG
DDRIASATFLTTQIDFTHAGDLKVFVDEDQLASIERKMKDMGYLEGSKMASAFNLLRSNDLIWPYVVNNYMKGKAPFPFD
LFFWNADSTRMPAANHSFYLRNCYLTNNIARGLAELGGVRIDVHKISVPVYSLATKEDHIAPPNSVYIGANLLSGPVRYV
LAGSGHIAGVVNPPAKGKYQYWADGPTGPSFDLWLEGARESAGSWWPDWISWFSAQHPDEVPARTIGGDRLTPIEDAPGR
YVRARS

Sequences:

>Translated_646_residues
MPAPHLTVVPPAPAEAPKPAAGKASETKVASAEGASAATSRARAPSPAASPAGGDGARSGMDVEVLARNLAQMMEEGGKA
MAAYLAPRHPGKTDDMAEDIADALKTVGHVAEYWMADPQRTVEAQSRLMAGYLSVWANTMKRLAGEEVTPVTKPDAKDAR
FKDQGWSDSPIFDALKQAYLVTAKWAEDMVEEAEGLDPHLKHKAEFLVRQVSNAISPSNFVMTNPELIRETMSSSGENLV
KGMRNLTADLIEGKGTLKIRQTDMSAFEVGRNLATTPGKVIYENELMQLIQYEATTASVKKTPLLIVPPWINKFYILDLT
AEKSFIKWLVDQGVSVFVISWVNPDARLATKGFDDYMREGIMTALDQVAIATGERQAHAMGYCVGGTLLATTLAYMAATG
DDRIASATFLTTQIDFTHAGDLKVFVDEDQLASIERKMKDMGYLEGSKMASAFNLLRSNDLIWPYVVNNYMKGKAPFPFD
LFFWNADSTRMPAANHSFYLRNCYLTNNIARGLAELGGVRIDVHKISVPVYSLATKEDHIAPPNSVYIGANLLSGPVRYV
LAGSGHIAGVVNPPAKGKYQYWADGPTGPSFDLWLEGARESAGSWWPDWISWFSAQHPDEVPARTIGGDRLTPIEDAPGR
YVRARS
>Mature_645_residues
PAPHLTVVPPAPAEAPKPAAGKASETKVASAEGASAATSRARAPSPAASPAGGDGARSGMDVEVLARNLAQMMEEGGKAM
AAYLAPRHPGKTDDMAEDIADALKTVGHVAEYWMADPQRTVEAQSRLMAGYLSVWANTMKRLAGEEVTPVTKPDAKDARF
KDQGWSDSPIFDALKQAYLVTAKWAEDMVEEAEGLDPHLKHKAEFLVRQVSNAISPSNFVMTNPELIRETMSSSGENLVK
GMRNLTADLIEGKGTLKIRQTDMSAFEVGRNLATTPGKVIYENELMQLIQYEATTASVKKTPLLIVPPWINKFYILDLTA
EKSFIKWLVDQGVSVFVISWVNPDARLATKGFDDYMREGIMTALDQVAIATGERQAHAMGYCVGGTLLATTLAYMAATGD
DRIASATFLTTQIDFTHAGDLKVFVDEDQLASIERKMKDMGYLEGSKMASAFNLLRSNDLIWPYVVNNYMKGKAPFPFDL
FFWNADSTRMPAANHSFYLRNCYLTNNIARGLAELGGVRIDVHKISVPVYSLATKEDHIAPPNSVYIGANLLSGPVRYVL
AGSGHIAGVVNPPAKGKYQYWADGPTGPSFDLWLEGARESAGSWWPDWISWFSAQHPDEVPARTIGGDRLTPIEDAPGRY
VRARS

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 70414; Mature: 70282

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPHLTVVPPAPAEAPKPAAGKASETKVASAEGASAATSRARAPSPAASPAGGDGARSG
CCCCCEEEECCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
MDVEVLARNLAQMMEEGGKAMAAYLAPRHPGKTDDMAEDIADALKTVGHVAEYWMADPQR
CCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
TVEAQSRLMAGYLSVWANTMKRLAGEEVTPVTKPDAKDARFKDQGWSDSPIFDALKQAYL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHE
VTAKWAEDMVEEAEGLDPHLKHKAEFLVRQVSNAISPSNFVMTNPELIRETMSSSGENLV
EHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCHHHH
KGMRNLTADLIEGKGTLKIRQTDMSAFEVGRNLATTPGKVIYENELMQLIQYEATTASVK
HHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHC
KTPLLIVPPWINKFYILDLTAEKSFIKWLVDQGVSVFVISWVNPDARLATKGFDDYMREG
CCCEEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCHHHHHHHH
IMTALDQVAIATGERQAHAMGYCVGGTLLATTLAYMAATGDDRIASATFLTTQIDFTHAG
HHHHHHHHHHHCCCCHHHHHEEEHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECC
DLKVFVDEDQLASIERKMKDMGYLEGSKMASAFNLLRSNDLIWPYVVNNYMKGKAPFPFD
CEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCEE
LFFWNADSTRMPAANHSFYLRNCYLTNNIARGLAELGGVRIDVHKISVPVYSLATKEDHI
EEEECCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCEEEEEEEEECCCEEECCCCCCC
APPNSVYIGANLLSGPVRYVLAGSGHIAGVVNPPAKGKYQYWADGPTGPSFDLWLEGARE
CCCCCEEEECHHHCCCEEEEEECCCCEEEEECCCCCCCEEEECCCCCCCCHHHEECCCHH
SAGSWWPDWISWFSAQHPDEVPARTIGGDRLTPIEDAPGRYVRARS
CCCCCCHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
PAPHLTVVPPAPAEAPKPAAGKASETKVASAEGASAATSRARAPSPAASPAGGDGARSG
CCCCEEEECCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
MDVEVLARNLAQMMEEGGKAMAAYLAPRHPGKTDDMAEDIADALKTVGHVAEYWMADPQR
CCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
TVEAQSRLMAGYLSVWANTMKRLAGEEVTPVTKPDAKDARFKDQGWSDSPIFDALKQAYL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHE
VTAKWAEDMVEEAEGLDPHLKHKAEFLVRQVSNAISPSNFVMTNPELIRETMSSSGENLV
EHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCHHHH
KGMRNLTADLIEGKGTLKIRQTDMSAFEVGRNLATTPGKVIYENELMQLIQYEATTASVK
HHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHC
KTPLLIVPPWINKFYILDLTAEKSFIKWLVDQGVSVFVISWVNPDARLATKGFDDYMREG
CCCEEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCHHHHHHHH
IMTALDQVAIATGERQAHAMGYCVGGTLLATTLAYMAATGDDRIASATFLTTQIDFTHAG
HHHHHHHHHHHCCCCHHHHHEEEHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECC
DLKVFVDEDQLASIERKMKDMGYLEGSKMASAFNLLRSNDLIWPYVVNNYMKGKAPFPFD
CEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCEE
LFFWNADSTRMPAANHSFYLRNCYLTNNIARGLAELGGVRIDVHKISVPVYSLATKEDHI
EEEECCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCEEEEEEEEECCCEEECCCCCCC
APPNSVYIGANLLSGPVRYVLAGSGHIAGVVNPPAKGKYQYWADGPTGPSFDLWLEGARE
CCCCCEEEECHHHCCCEEEEEECCCCEEEEECCCCCCCEEEECCCCCCCCHHHEECCCHH
SAGSWWPDWISWFSAQHPDEVPARTIGGDRLTPIEDAPGRYVRARS
CCCCCCHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]