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The map label for this gene is def [H]

Identifier: 154247233

GI number: 154247233

Start: 3674481

End: 3674999

Strand: Reverse

Name: def [H]

Synonym: Xaut_3305

Alternate gene names: 154247233

Gene position: 3674999-3674481 (Counterclockwise)

Preceding gene: 154247237

Following gene: 154247232

Centisome position: 69.22

GC content: 63.39

Gene sequence:

>519_bases
ATGGCGACGCGACCCATCCTCATCATCCCCGAGCCCAAGCTGCGCACCCTCTCGGCATCGGTCGAGAAAATCGACAGCGA
GGTGAAGAAGCTCGTGGAAGACATGTTCGACACCATGTACGACGCGCCGGGCATCGGCCTCGCGGCGATCCAGGTGGGCG
TGCAGCGACGCGTCGTCACCATCGATGTCGCGCGCGAGGGCGAGGCGAAGAAGCCCATCGCCCTCATCAATCCCGAAATC
ATCGCAGCATCCGAGGAGACCTCGGTCTATGCCGAGGGCTGCCTCTCCATCCCCGAATATTACGAGGAGGTGGAACGGCC
GGCGCGGGTCACCGTGCGCTTCCAGGACATCGAGGGGCAGGTGCGCGAAGTGGCGGCGGACGGCCTGTTCGCCACCTGCG
TGCAGCACGAGATCGATCATCTGAACGGCGTGCTCTTCATCGACCACATCTCGAAGCTGAAGCGCGACCGGGTGATCAAG
AAGTTCACCAAGCTGGCGCGGCACAAGGACGAGGTCTGA

Upstream 100 bases:

>100_bases
CTGAATAGGGCCTCCCGCATGGCCCCTTCTATCGCGATTCTCGATCCGAACAAACAGCGAACATATTGACCGGACGCGAA
GCTTCGCTTATTTCGCGAGA

Downstream 100 bases:

>100_bases
CGCCGCGGGCGCCGGCGCCGCCTTTCCCGCTTTCCCGCTGATGCCGTCGCATCCGGGTCCTTTCGCTCAATCGCCGCGCA
CCTGACTGGACGGGTCGCGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MATRPILIIPEPKLRTLSASVEKIDSEVKKLVEDMFDTMYDAPGIGLAAIQVGVQRRVVTIDVAREGEAKKPIALINPEI
IAASEETSVYAEGCLSIPEYYEEVERPARVTVRFQDIEGQVREVAADGLFATCVQHEIDHLNGVLFIDHISKLKRDRVIK
KFTKLARHKDEV

Sequences:

>Translated_172_residues
MATRPILIIPEPKLRTLSASVEKIDSEVKKLVEDMFDTMYDAPGIGLAAIQVGVQRRVVTIDVAREGEAKKPIALINPEI
IAASEETSVYAEGCLSIPEYYEEVERPARVTVRFQDIEGQVREVAADGLFATCVQHEIDHLNGVLFIDHISKLKRDRVIK
KFTKLARHKDEV
>Mature_171_residues
ATRPILIIPEPKLRTLSASVEKIDSEVKKLVEDMFDTMYDAPGIGLAAIQVGVQRRVVTIDVAREGEAKKPIALINPEII
AASEETSVYAEGCLSIPEYYEEVERPARVTVRFQDIEGQVREVAADGLFATCVQHEIDHLNGVLFIDHISKLKRDRVIKK
FTKLARHKDEV

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=44.9704142011834, Blast_Score=148, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24645728, Length=160, Percent_Identity=32.5, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24645726, Length=161, Percent_Identity=31.055900621118, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19282; Mature: 19150

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRPILIIPEPKLRTLSASVEKIDSEVKKLVEDMFDTMYDAPGIGLAAIQVGVQRRVVT
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEE
IDVAREGEAKKPIALINPEIIAASEETSVYAEGCLSIPEYYEEVERPARVTVRFQDIEGQ
EEECCCCCCCCCEEEECCCEEECCCCCHHHHHHHCCCHHHHHHHCCCCEEEEEEECCHHH
VREVAADGLFATCVQHEIDHLNGVLFIDHISKLKRDRVIKKFTKLARHKDEV
HHHHHHCCHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATRPILIIPEPKLRTLSASVEKIDSEVKKLVEDMFDTMYDAPGIGLAAIQVGVQRRVVT
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEE
IDVAREGEAKKPIALINPEIIAASEETSVYAEGCLSIPEYYEEVERPARVTVRFQDIEGQ
EEECCCCCCCCCEEEECCCEEECCCCCHHHHHHHCCCHHHHHHHCCCCEEEEEEECCHHH
VREVAADGLFATCVQHEIDHLNGVLFIDHISKLKRDRVIKKFTKLARHKDEV
HHHHHHCCHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]