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The map label for this gene is truA

Identifier: 154247231

GI number: 154247231

Start: 3672682

End: 3673419

Strand: Reverse

Name: truA

Synonym: Xaut_3303

Alternate gene names: 154247231

Gene position: 3673419-3672682 (Counterclockwise)

Preceding gene: 154247232

Following gene: 154247230

Centisome position: 69.19

GC content: 68.97

Gene sequence:

>738_bases
ATGCCGCGCTACAAGCTGACCATCGAATATGATGGCACCCCCTATGCCGGCTGGCAGATCCAGGCCGACCAGCAGACCGT
GCAGGGCGCGCTGGCGCAGGCATTCAAGCGTTTCTGCGGCGAGGATGTGCATGTGGCCGGCGCCGGGCGCACTGATGCGG
GGGTGCATGCCACCGGGCAGGTGGCCCATGTCGACCTGTCCCGGCACTGGCGCAGCGACACGGTGCGCGACGCCATGACC
GCGCAGCTGCGTCCCCACCCCATCGCAGTTCTGAGCGCCGAAGAGGTGCCGGACAGCTTCGACGCCCGCTTCTCCGCCAC
CAAGCGGCATTATCTCTACCGTATCATCAATCGGCGGCCGGACCTCGCCCTCGACCGGGACCGGGCCTGGCGCATTCCGC
AGAAGCTGGACGCCCAGGCCATGCATGAGGCCGCCCAGCGGCTTTTGGGCAAGCACGATTTCTCCACCTTCCGCGCCGCC
GAATGCCAGGCCGCCTCGCCGCTCAAGACCCTGGACCAGCTGGACGTTTCCCGCTCCGGCGACGAGATCCGCGTCATCAC
CTCGGCGCGGTCCTTCCTGCACCATCAGGTGCGCTCCATGGTGGGCTCGCTGATGCGGGTGGGCGAAGGGCGCTGGAGCG
CCGACGACCTGGAAGCCGCCCTGAAAGCCGCCGACCGCACCCGCTGCGGCCCCATGGCGCCCGCCGCCGGGCTCTATCTG
GCCGCCGTCAGCTATTGA

Upstream 100 bases:

>100_bases
CAGAAGGCCGGCAAGCAGCCGGCGGGTGCGTCGAGCTTTCTCAACGGGAATGCCGTGCCGCCCGGCACCCGGCTCACCTG
AGCCGCCGGCTCGCCCCGCC

Downstream 100 bases:

>100_bases
GCGCGCGCAAGCCTCCGGACCGCGTCATCAACCCTTTGTAAGCACTACCTGCCGCCAAGCAGGGAACGTCTGCCCCGCCG
GGGGCATTCCCTCTTCAAAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPRYKLTIEYDGTPYAGWQIQADQQTVQGALAQAFKRFCGEDVHVAGAGRTDAGVHATGQVAHVDLSRHWRSDTVRDAMT
AQLRPHPIAVLSAEEVPDSFDARFSATKRHYLYRIINRRPDLALDRDRAWRIPQKLDAQAMHEAAQRLLGKHDFSTFRAA
ECQAASPLKTLDQLDVSRSGDEIRVITSARSFLHHQVRSMVGSLMRVGEGRWSADDLEAALKAADRTRCGPMAPAAGLYL
AAVSY

Sequences:

>Translated_245_residues
MPRYKLTIEYDGTPYAGWQIQADQQTVQGALAQAFKRFCGEDVHVAGAGRTDAGVHATGQVAHVDLSRHWRSDTVRDAMT
AQLRPHPIAVLSAEEVPDSFDARFSATKRHYLYRIINRRPDLALDRDRAWRIPQKLDAQAMHEAAQRLLGKHDFSTFRAA
ECQAASPLKTLDQLDVSRSGDEIRVITSARSFLHHQVRSMVGSLMRVGEGRWSADDLEAALKAADRTRCGPMAPAAGLYL
AAVSY
>Mature_244_residues
PRYKLTIEYDGTPYAGWQIQADQQTVQGALAQAFKRFCGEDVHVAGAGRTDAGVHATGQVAHVDLSRHWRSDTVRDAMTA
QLRPHPIAVLSAEEVPDSFDARFSATKRHYLYRIINRRPDLALDRDRAWRIPQKLDAQAMHEAAQRLLGKHDFSTFRAAE
CQAASPLKTLDQLDVSRSGDEIRVITSARSFLHHQVRSMVGSLMRVGEGRWSADDLEAALKAADRTRCGPMAPAAGLYLA
AVSY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=35.8695652173913, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=43.4959349593496, Blast_Score=182, Evalue=2e-47,
Organism=Drosophila melanogaster, GI116007752, Length=301, Percent_Identity=30.2325581395349, Blast_Score=104, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_XANP2 (A7IKI9)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001418189.1
- ProteinModelPortal:   A7IKI9
- SMR:   A7IKI9
- STRING:   A7IKI9
- GeneID:   5421639
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_3303
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   XAUT78245:XAUT_3303-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27209; Mature: 27078

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYKLTIEYDGTPYAGWQIQADQQTVQGALAQAFKRFCGEDVHVAGAGRTDAGVHATGQ
CCCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
VAHVDLSRHWRSDTVRDAMTAQLRPHPIAVLSAEEVPDSFDARFSATKRHYLYRIINRRP
EEEEEHHHHHCHHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCC
DLALDRDRAWRIPQKLDAQAMHEAAQRLLGKHDFSTFRAAECQAASPLKTLDQLDVSRSG
CCEECCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCC
DEIRVITSARSFLHHQVRSMVGSLMRVGEGRWSADDLEAALKAADRTRCGPMAPAAGLYL
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AAVSY
EEECC
>Mature Secondary Structure 
PRYKLTIEYDGTPYAGWQIQADQQTVQGALAQAFKRFCGEDVHVAGAGRTDAGVHATGQ
CCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
VAHVDLSRHWRSDTVRDAMTAQLRPHPIAVLSAEEVPDSFDARFSATKRHYLYRIINRRP
EEEEEHHHHHCHHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCC
DLALDRDRAWRIPQKLDAQAMHEAAQRLLGKHDFSTFRAAECQAASPLKTLDQLDVSRSG
CCEECCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCC
DEIRVITSARSFLHHQVRSMVGSLMRVGEGRWSADDLEAALKAADRTRCGPMAPAAGLYL
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AAVSY
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA