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The map label for this gene is yeaT [C]

Identifier: 154247221

GI number: 154247221

Start: 3656295

End: 3657308

Strand: Reverse

Name: yeaT [C]

Synonym: Xaut_3293

Alternate gene names: 154247221

Gene position: 3657308-3656295 (Counterclockwise)

Preceding gene: 154247230

Following gene: 154247219

Centisome position: 68.89

GC content: 69.13

Gene sequence:

>1014_bases
TTGTTGATCCGGGATCTTGCGGCACCATTGGCCCCGCCGTCCCGCGTGCTTATCGTTGCGAAGGTTCAGGGCAAAGGACG
CGGCATCGTGCTCGACAATATGAAGGCCTTCGCCATGTCGGTGGAGAGCGAGAGCTTCTCGGAAGCGGCGCGGCGGCTGC
GCCTGTCCGCCAATGTGGTGAGCCACCGCATCCAGATGCTGGAGAAGCACCTGGGCTGCCGGCTGTTCAACCGCACCACC
CGCCGCATGAGCCTGACCGAGCAGGGCCGGGTCTATTACGAGGCCATCACCGACGCGCTCCGCCTCATCGACGCGGCGGA
GAGCAATGTGTCGGAGCTGGGCGCCCTGCCCCGCGGCGCGCTCAGGGTGACCGCCCCGCTGCATCTGGGCCGGCGCCTGG
TGGCGCAGGTTTCGGCGCGCTACCAGGAGGCGCATCCGGAGATCGACGTGCGCCTGCGCCTCTCCGAGCACACCCTCGAC
CTCATCGCCGAATCCATCGACGTGGCGCTGCGCCTCGCCACCTTCGAGGATTCCAGCATGATCATGCGCAAGGTGGCGCA
GGTGGAACGCATCCTGTGCGCCGCCCCGGCCTATCTGGAGCGGGCGGGAGTGCCGCTGCGCCCGCAGGATCTGTTGTCCC
ACCAATGCCTGCTCCTGAGATTTGCGAGCCTTCGGGAACTGCGCTGGATGCTGACCGACGAGGGCCGGGACCTCGCCGTG
CCGGTCACCGGCCACCTGGAGGCCGACGATGGCGACGTGCTGACGGTGTGGGCGCTCGAGGGGCGGGGCATCGTGGTGAA
GCCCCGCTTCGAGGTGGCGGATGCGCTCGCCGAGGGCCGCCTGGTGCCGGTGCTTCAGGCGCACCCGCCCAAGCCCGCGA
CCCTCGCCGTGCTCTACCCGGCCCGCCAGCTGGTGCCGTTGAAGGTGAAGGCCTTCGCCGACATGCTGGTGGACCAGGGC
CGCCGCTACATCGCCCAGGAGCTGGGGAAGATCGGGGAGCGGCTGTCCGCCTGA

Upstream 100 bases:

>100_bases
TTCAGGTCCCGTAGCTGGAAGGCGAAGGGGCATTTCCTTCGGAGCCCGCGCCCATGGGCTTGCGCCGCAAGCACATGGGA
TGACGCGGTCTCTCATATGA

Downstream 100 bases:

>100_bases
GCGCAAAGCCAAGCCCTGATTGGCCGCGTTCATCCTTGCACCGCTCCCCTTTCCCCGCCCCCGGCCCACGTCAGTGGGCC
TCGGCCTGCTTGGCCGCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MLIRDLAAPLAPPSRVLIVAKVQGKGRGIVLDNMKAFAMSVESESFSEAARRLRLSANVVSHRIQMLEKHLGCRLFNRTT
RRMSLTEQGRVYYEAITDALRLIDAAESNVSELGALPRGALRVTAPLHLGRRLVAQVSARYQEAHPEIDVRLRLSEHTLD
LIAESIDVALRLATFEDSSMIMRKVAQVERILCAAPAYLERAGVPLRPQDLLSHQCLLLRFASLRELRWMLTDEGRDLAV
PVTGHLEADDGDVLTVWALEGRGIVVKPRFEVADALAEGRLVPVLQAHPPKPATLAVLYPARQLVPLKVKAFADMLVDQG
RRYIAQELGKIGERLSA

Sequences:

>Translated_337_residues
MLIRDLAAPLAPPSRVLIVAKVQGKGRGIVLDNMKAFAMSVESESFSEAARRLRLSANVVSHRIQMLEKHLGCRLFNRTT
RRMSLTEQGRVYYEAITDALRLIDAAESNVSELGALPRGALRVTAPLHLGRRLVAQVSARYQEAHPEIDVRLRLSEHTLD
LIAESIDVALRLATFEDSSMIMRKVAQVERILCAAPAYLERAGVPLRPQDLLSHQCLLLRFASLRELRWMLTDEGRDLAV
PVTGHLEADDGDVLTVWALEGRGIVVKPRFEVADALAEGRLVPVLQAHPPKPATLAVLYPARQLVPLKVKAFADMLVDQG
RRYIAQELGKIGERLSA
>Mature_337_residues
MLIRDLAAPLAPPSRVLIVAKVQGKGRGIVLDNMKAFAMSVESESFSEAARRLRLSANVVSHRIQMLEKHLGCRLFNRTT
RRMSLTEQGRVYYEAITDALRLIDAAESNVSELGALPRGALRVTAPLHLGRRLVAQVSARYQEAHPEIDVRLRLSEHTLD
LIAESIDVALRLATFEDSSMIMRKVAQVERILCAAPAYLERAGVPLRPQDLLSHQCLLLRFASLRELRWMLTDEGRDLAV
PVTGHLEADDGDVLTVWALEGRGIVVKPRFEVADALAEGRLVPVLQAHPPKPATLAVLYPARQLVPLKVKAFADMLVDQG
RRYIAQELGKIGERLSA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=29.5302013422819, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789639, Length=305, Percent_Identity=29.8360655737705, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789440, Length=297, Percent_Identity=30.976430976431, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=30.9027777777778, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI145693193, Length=309, Percent_Identity=30.0970873786408, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=32.0689655172414, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=28.9655172413793, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1790011, Length=306, Percent_Identity=24.8366013071895, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=28.3464566929134, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788748, Length=136, Percent_Identity=30.1470588235294, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=155, Percent_Identity=26.4516129032258, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788706, Length=272, Percent_Identity=26.8382352941176, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786448, Length=259, Percent_Identity=26.2548262548263, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=173, Percent_Identity=27.7456647398844, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37371; Mature: 37371

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIRDLAAPLAPPSRVLIVAKVQGKGRGIVLDNMKAFAMSVESESFSEAARRLRLSANVV
CCCHHHHCCCCCCCEEEEEEEECCCCCEEEEECCHHHHEECCCHHHHHHHHHHHHHHHHH
SHRIQMLEKHLGCRLFNRTTRRMSLTEQGRVYYEAITDALRLIDAAESNVSELGALPRGA
HHHHHHHHHHHCHHHHHHHHHHHEHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
LRVTAPLHLGRRLVAQVSARYQEAHPEIDVRLRLSEHTLDLIAESIDVALRLATFEDSSM
EEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHEEEEEECCCHHH
IMRKVAQVERILCAAPAYLERAGVPLRPQDLLSHQCLLLRFASLRELRWMLTDEGRDLAV
HHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
PVTGHLEADDGDVLTVWALEGRGIVVKPRFEVADALAEGRLVPVLQAHPPKPATLAVLYP
EEECCEECCCCCEEEEEEECCCEEEEECCHHHHHHHHCCCEEEEEECCCCCCCEEEEEEC
ARQLVPLKVKAFADMLVDQGRRYIAQELGKIGERLSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIRDLAAPLAPPSRVLIVAKVQGKGRGIVLDNMKAFAMSVESESFSEAARRLRLSANVV
CCCHHHHCCCCCCCEEEEEEEECCCCCEEEEECCHHHHEECCCHHHHHHHHHHHHHHHHH
SHRIQMLEKHLGCRLFNRTTRRMSLTEQGRVYYEAITDALRLIDAAESNVSELGALPRGA
HHHHHHHHHHHCHHHHHHHHHHHEHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
LRVTAPLHLGRRLVAQVSARYQEAHPEIDVRLRLSEHTLDLIAESIDVALRLATFEDSSM
EEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHEEEEEECCCHHH
IMRKVAQVERILCAAPAYLERAGVPLRPQDLLSHQCLLLRFASLRELRWMLTDEGRDLAV
HHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
PVTGHLEADDGDVLTVWALEGRGIVVKPRFEVADALAEGRLVPVLQAHPPKPATLAVLYP
EEECCEECCCCCEEEEEEECCCEEEEECCHHHHHHHHCCCEEEEEECCCCCCCEEEEEEC
ARQLVPLKVKAFADMLVDQGRRYIAQELGKIGERLSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]