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The map label for this gene is amaB [H]

Identifier: 154247217

GI number: 154247217

Start: 3651490

End: 3652770

Strand: Reverse

Name: amaB [H]

Synonym: Xaut_3289

Alternate gene names: 154247217

Gene position: 3652770-3651490 (Counterclockwise)

Preceding gene: 154247218

Following gene: 154247216

Centisome position: 68.8

GC content: 70.49

Gene sequence:

>1281_bases
ATGAACAAGGTCTTCCCCGTGCCCCAACCTGCCCCCGTCCCCACCCTCGCGCCCCTTGACGGCAATGCCATCACCGCCCG
CCTGAAGGAGCTTGCCGCCTTCTCGGACGTGCCGGGCGAACTCACCCGCCTCTCCCTCACCCCGTCCCACAAGCGGGCGG
CGGCGCAGGTATCCCACTGGATGCGCGAGGCCGGCCTCACCCAGGTCCACATGGACGCCACCGGCACCATCGTTGGGCGC
TATCCGGCAGACCGGGCCAATGCCAAGACCCTGCTCATCGGCTCGCACATCGACACGGTGCGCAACGCCGGCATCTATGA
CGGGAACCTCGGCGTGCTCACCGCCATCGCGGCGGTGGAAGCGCTGAACGCGCAGGGCCTGCGCCTGCCGTTCGCCATCG
AGGTGGCGGCCTTCGCGGATGAAGAGGGCGTGCGCTTCCTCTCCACCCTCACCTCATCCAAGGCCATGGCGGGCATCTTC
GATCCCAAGAGCCTTGATGACGTGGACCAGCAGGGCATCTCCCGCGCCGAGGCGCTGCGCTCGTTCGGCGCGCCGGCGGA
GCGTGTCGCCGAATGCCGGCGCGATCCGGCGGACATGATCGGCTATGTGGAAGTGCATATCGAGCAGGGGCCGGTGCTGG
AAGCGGCGGGCGCTCCGCTGGGCATCGTCACCGGCATCGCCGGCGCCTCGCGCGGGCACATTCGCGTGCGCGGCGAGGCC
GGCCATTCCGGCACCCTGCCCATGCCCATGCGCCACGACGCGCTGGCCGCCGCAGCCGAGATGATCCTCGCCGTCGAAGC
CCGCGGCCGCGCCGATGCCGATGCCCTGGTGACCACAGTCGGCACCATGATCATCGCCGGTAGCGCCGTGAACGTGGTGC
CGGGCGAGGTGCAATTCTCCCTCGACGTGCGCACCCTCTCCGACGATCTGCGCCAGCGCGCCGTCGCCGACATCCGCGAG
GCCATCGACGAGATCGCGGCGCGGCGCGGCGTGCGCGCCGTCCTCGACATCGGCCATGAGGTGCCGGCCGCGCCCTGCCA
CGGCGTCCTGAAGGAGCGCATGGCGCAGGTGGTGGAAGGCCTGCAACTGCCCGTGGTGCGCCTGCCCTCCGGCGCCGGGC
ACGACGCCATGGTCTTCCGGGGCATCATGCCCATGGCCATGCTGTTCGTGCGCTCCGAGAACGGCAGTCACAACCCGCGC
GAATACGCCTCCCCCGCCGACATCGGCCTTGCCGCCGAAGCGCTCCATGCCTTCCTGCTGAAGACCGCCGGGGAGGTGTG
A

Upstream 100 bases:

>100_bases
GCCGGCTCGCCCATGATCGCGACCAGGAGGTCAACGAGGCCCTCACACAGATCGAGCGCATCGCCCGGCTCCGGCTGGAC
CAGATGCTCCCGGAGGCCGC

Downstream 100 bases:

>100_bases
GCCGGTTGCGTCCCCCGCTGGTGCGGTATCTCCGCGCCCGCGAGCCTCAGGCCGATCACGCGGGCTGGAAAGAGAGGCAA
TGTGTTTCGGGCCGCGAAAC

Product: amidase

Products: NA

Alternate protein names: L-carbamoylase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNKVFPVPQPAPVPTLAPLDGNAITARLKELAAFSDVPGELTRLSLTPSHKRAAAQVSHWMREAGLTQVHMDATGTIVGR
YPADRANAKTLLIGSHIDTVRNAGIYDGNLGVLTAIAAVEALNAQGLRLPFAIEVAAFADEEGVRFLSTLTSSKAMAGIF
DPKSLDDVDQQGISRAEALRSFGAPAERVAECRRDPADMIGYVEVHIEQGPVLEAAGAPLGIVTGIAGASRGHIRVRGEA
GHSGTLPMPMRHDALAAAAEMILAVEARGRADADALVTTVGTMIIAGSAVNVVPGEVQFSLDVRTLSDDLRQRAVADIRE
AIDEIAARRGVRAVLDIGHEVPAAPCHGVLKERMAQVVEGLQLPVVRLPSGAGHDAMVFRGIMPMAMLFVRSENGSHNPR
EYASPADIGLAAEALHAFLLKTAGEV

Sequences:

>Translated_426_residues
MNKVFPVPQPAPVPTLAPLDGNAITARLKELAAFSDVPGELTRLSLTPSHKRAAAQVSHWMREAGLTQVHMDATGTIVGR
YPADRANAKTLLIGSHIDTVRNAGIYDGNLGVLTAIAAVEALNAQGLRLPFAIEVAAFADEEGVRFLSTLTSSKAMAGIF
DPKSLDDVDQQGISRAEALRSFGAPAERVAECRRDPADMIGYVEVHIEQGPVLEAAGAPLGIVTGIAGASRGHIRVRGEA
GHSGTLPMPMRHDALAAAAEMILAVEARGRADADALVTTVGTMIIAGSAVNVVPGEVQFSLDVRTLSDDLRQRAVADIRE
AIDEIAARRGVRAVLDIGHEVPAAPCHGVLKERMAQVVEGLQLPVVRLPSGAGHDAMVFRGIMPMAMLFVRSENGSHNPR
EYASPADIGLAAEALHAFLLKTAGEV
>Mature_426_residues
MNKVFPVPQPAPVPTLAPLDGNAITARLKELAAFSDVPGELTRLSLTPSHKRAAAQVSHWMREAGLTQVHMDATGTIVGR
YPADRANAKTLLIGSHIDTVRNAGIYDGNLGVLTAIAAVEALNAQGLRLPFAIEVAAFADEEGVRFLSTLTSSKAMAGIF
DPKSLDDVDQQGISRAEALRSFGAPAERVAECRRDPADMIGYVEVHIEQGPVLEAAGAPLGIVTGIAGASRGHIRVRGEA
GHSGTLPMPMRHDALAAAAEMILAVEARGRADADALVTTVGTMIIAGSAVNVVPGEVQFSLDVRTLSDDLRQRAVADIRE
AIDEIAARRGVRAVLDIGHEVPAAPCHGVLKERMAQVVEGLQLPVVRLPSGAGHDAMVFRGIMPMAMLFVRSENGSHNPR
EYASPADIGLAAEALHAFLLKTAGEV

Specific function: Involved In The Anaerobic Utilization Of Allantoin. Converts Allantoate To (S)-Ureidoglycolate And Ammonia. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=410, Percent_Identity=31.9512195121951, Blast_Score=188, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.87 [H]

Molecular weight: Translated: 44813; Mature: 44813

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVFPVPQPAPVPTLAPLDGNAITARLKELAAFSDVPGELTRLSLTPSHKRAAAQVSHW
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHH
MREAGLTQVHMDATGTIVGRYPADRANAKTLLIGSHIDTVRNAGIYDGNLGVLTAIAAVE
HHHCCCCEEEECCCCEEEECCCCCCCCCCEEEEECCHHHHHCCCCCCCCHHHHHHHHHHH
ALNAQGLRLPFAIEVAAFADEEGVRFLSTLTSSKAMAGIFDPKSLDDVDQQGISRAEALR
HHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SFGAPAERVAECRRDPADMIGYVEVHIEQGPVLEAAGAPLGIVTGIAGASRGHIRVRGEA
HHCCCHHHHHHHHCCHHHHEEEEEEEECCCCEEECCCCCHHHHHHCCCCCCCEEEEEECC
GHSGTLPMPMRHDALAAAAEMILAVEARGRADADALVTTVGTMIIAGSAVNVVPGEVQFS
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCEEEE
LDVRTLSDDLRQRAVADIREAIDEIAARRGVRAVLDIGHEVPAAPCHGVLKERMAQVVEG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
LQLPVVRLPSGAGHDAMVFRGIMPMAMLFVRSENGSHNPREYASPADIGLAAEALHAFLL
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHH
KTAGEV
HHCCCC
>Mature Secondary Structure
MNKVFPVPQPAPVPTLAPLDGNAITARLKELAAFSDVPGELTRLSLTPSHKRAAAQVSHW
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHH
MREAGLTQVHMDATGTIVGRYPADRANAKTLLIGSHIDTVRNAGIYDGNLGVLTAIAAVE
HHHCCCCEEEECCCCEEEECCCCCCCCCCEEEEECCHHHHHCCCCCCCCHHHHHHHHHHH
ALNAQGLRLPFAIEVAAFADEEGVRFLSTLTSSKAMAGIFDPKSLDDVDQQGISRAEALR
HHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SFGAPAERVAECRRDPADMIGYVEVHIEQGPVLEAAGAPLGIVTGIAGASRGHIRVRGEA
HHCCCHHHHHHHHCCHHHHEEEEEEEECCCCEEECCCCCHHHHHHCCCCCCCEEEEEECC
GHSGTLPMPMRHDALAAAAEMILAVEARGRADADALVTTVGTMIIAGSAVNVVPGEVQFS
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCEEEE
LDVRTLSDDLRQRAVADIREAIDEIAARRGVRAVLDIGHEVPAAPCHGVLKERMAQVVEG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
LQLPVVRLPSGAGHDAMVFRGIMPMAMLFVRSENGSHNPREYASPADIGLAAEALHAFLL
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHH
KTAGEV
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9023955; 8285691 [H]