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The map label for this gene is pucL [H]

Identifier: 154247218

GI number: 154247218

Start: 3652767

End: 3654215

Strand: Reverse

Name: pucL [H]

Synonym: Xaut_3290

Alternate gene names: 154247218

Gene position: 3654215-3652767 (Counterclockwise)

Preceding gene: 154247219

Following gene: 154247217

Centisome position: 68.83

GC content: 67.91

Gene sequence:

>1449_bases
ATGAGCGTGCCCAACGATCCCATCACCGGCCGCCCCTACCCGCGCGACCTGATCGGCTACGGACGCAATCCGCCCCATGC
GCGCTGGCCGGGAGAGGCGCGCATCGCCGTCCAGTTCGTGGTGAATTACGAGGAGGGTGGCGAGAACTCCATCCTCCACG
GCGACAATGCCTCGGAAGCCTTCCTGTCCGAGATCATCGGCGCCCAGCCCTGGCCCCGCCAGCGCCACATGAACATGGAA
TCCATCTACGAATACGGCGCGCGGGTGGGCTTCTGGCGGCTGCACCGCCTGTTCACCGAGCGCGCCATGCCGGTCACGGT
CTATGGCGTCGCCACGGCGCTGGCCCGCAACCCGGACGCCGTCGCCGCCATGAAAGAGGCCGACTGGGAGATCGCGAGCC
ACGGCTACAAATGGCTCGAATACAAGGACTTCACCCGCGACGCCGAGCGCGAGCACATCGAGGAGGCGATCCGCATCCAC
ACCGAAGTGACCGGCTCCCGCCCGCTGGGTTTCTACCAGGGCCGCTCGGCCGAGCACACGGTGCCATTGGTGATGGAGGA
AGGCGGCTTCCTCTATGCCGCGGACAGCTATGCGGATGAACTGCCCTACTGGATCGAGAGTGCCAAGGGTCCGCAACTGG
TGGTGCCCTATACGCTGGACGCCAACGACATGCGCTTCTCGGTGGCGGCGGGCTTCGGCACCGGCACGGATTTCTTCGAC
TATCTGAAAGACAGCTTCGACCTGCTCTATGCCGAGGGCGAGACTGCGCCGAAGATGATGTCGGTGGGCCTCCACCCCCG
CCTCGCCGGCCGGCCCGGCCGCGCCCGGGCGCTGGCCCGCTTCCTCGATTACATCGCCGGCCATGAGAAGGTTTGGGTGG
CCCGGCGCGTGGACATCGCCCGCCACTGGATCGCCACCCACCCCCCCGTCGGCGGCTACAAGCCCACCTTCATGCCGCGC
GGCCTGTTCGTGGAGCGGTTCGGCGGCGTGTTCGAGCATTCACCCTGGATCGCGGAAGCCGCGTACGACAAGGGTCTCAC
CCCCCGCGAGGACACCGCCGCCGGCCTCCACGCGGCGCTTGCCGCCGTGCTGCGCGCCGCCCCCATCGAGAAGCAGATGG
GGGTCATCCACGCCCATCCGGACCTGGCAGGCAAGCTGGCCGAGGCCAAGCTGCTCACCGCCGAATCCACCGCCGAGCAG
GCCTCGGCCGGCCTCGACCGGCTGACGGCGGAGGAGAAGGCCCGCTTCACCGCCTTCAATGACGCCTACGTGGCAAAGTT
CGGATTCGTCTTCATCATGGCCATCCGCGGCCGGACCAAGGCCGAGATCCTCGATGCCTTCGAGCGCCGGCTCGCCCATG
ATCGCGACCAGGAGGTCAACGAGGCCCTCACACAGATCGAGCGCATCGCCCGGCTCCGGCTGGACCAGATGCTCCCGGAG
GCCGCATGA

Upstream 100 bases:

>100_bases
TGTGGTGCCCCTGCGCTTCTCGCTGGCGGAGGACGGGCACTATCATGTGCCGCTGCTGTGCTCCCCGTGGAGCTACTCCA
CCTATCGGGGCTCCTGAGCG

Downstream 100 bases:

>100_bases
ACAAGGTCTTCCCCGTGCCCCAACCTGCCCCCGTCCCCACCCTCGCGCCCCTTGACGGCAATGCCATCACCGCCCGCCTG
AAGGAGCTTGCCGCCTTCTC

Product: chitin deacetylase

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSVPNDPITGRPYPRDLIGYGRNPPHARWPGEARIAVQFVVNYEEGGENSILHGDNASEAFLSEIIGAQPWPRQRHMNME
SIYEYGARVGFWRLHRLFTERAMPVTVYGVATALARNPDAVAAMKEADWEIASHGYKWLEYKDFTRDAEREHIEEAIRIH
TEVTGSRPLGFYQGRSAEHTVPLVMEEGGFLYAADSYADELPYWIESAKGPQLVVPYTLDANDMRFSVAAGFGTGTDFFD
YLKDSFDLLYAEGETAPKMMSVGLHPRLAGRPGRARALARFLDYIAGHEKVWVARRVDIARHWIATHPPVGGYKPTFMPR
GLFVERFGGVFEHSPWIAEAAYDKGLTPREDTAAGLHAALAAVLRAAPIEKQMGVIHAHPDLAGKLAEAKLLTAESTAEQ
ASAGLDRLTAEEKARFTAFNDAYVAKFGFVFIMAIRGRTKAEILDAFERRLAHDRDQEVNEALTQIERIARLRLDQMLPE
AA

Sequences:

>Translated_482_residues
MSVPNDPITGRPYPRDLIGYGRNPPHARWPGEARIAVQFVVNYEEGGENSILHGDNASEAFLSEIIGAQPWPRQRHMNME
SIYEYGARVGFWRLHRLFTERAMPVTVYGVATALARNPDAVAAMKEADWEIASHGYKWLEYKDFTRDAEREHIEEAIRIH
TEVTGSRPLGFYQGRSAEHTVPLVMEEGGFLYAADSYADELPYWIESAKGPQLVVPYTLDANDMRFSVAAGFGTGTDFFD
YLKDSFDLLYAEGETAPKMMSVGLHPRLAGRPGRARALARFLDYIAGHEKVWVARRVDIARHWIATHPPVGGYKPTFMPR
GLFVERFGGVFEHSPWIAEAAYDKGLTPREDTAAGLHAALAAVLRAAPIEKQMGVIHAHPDLAGKLAEAKLLTAESTAEQ
ASAGLDRLTAEEKARFTAFNDAYVAKFGFVFIMAIRGRTKAEILDAFERRLAHDRDQEVNEALTQIERIARLRLDQMLPE
AA
>Mature_481_residues
SVPNDPITGRPYPRDLIGYGRNPPHARWPGEARIAVQFVVNYEEGGENSILHGDNASEAFLSEIIGAQPWPRQRHMNMES
IYEYGARVGFWRLHRLFTERAMPVTVYGVATALARNPDAVAAMKEADWEIASHGYKWLEYKDFTRDAEREHIEEAIRIHT
EVTGSRPLGFYQGRSAEHTVPLVMEEGGFLYAADSYADELPYWIESAKGPQLVVPYTLDANDMRFSVAAGFGTGTDFFDY
LKDSFDLLYAEGETAPKMMSVGLHPRLAGRPGRARALARFLDYIAGHEKVWVARRVDIARHWIATHPPVGGYKPTFMPRG
LFVERFGGVFEHSPWIAEAAYDKGLTPREDTAAGLHAALAAVLRAAPIEKQMGVIHAHPDLAGKLAEAKLLTAESTAEQA
SAGLDRLTAEEKARFTAFNDAYVAKFGFVFIMAIRGRTKAEILDAFERRLAHDRDQEVNEALTQIERIARLRLDQMLPEA
A

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=166, Percent_Identity=35.5421686746988, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 53900; Mature: 53768

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPNDPITGRPYPRDLIGYGRNPPHARWPGEARIAVQFVVNYEEGGENSILHGDNASEA
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEECCCCCHHH
FLSEIIGAQPWPRQRHMNMESIYEYGARVGFWRLHRLFTERAMPVTVYGVATALARNPDA
HHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCHH
VAAMKEADWEIASHGYKWLEYKDFTRDAEREHIEEAIRIHTEVTGSRPLGFYQGRSAEHT
HHHHHHCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VPLVMEEGGFLYAADSYADELPYWIESAKGPQLVVPYTLDANDMRFSVAAGFGTGTDFFD
EEEEEECCCEEEEECCHHHHCCHHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHH
YLKDSFDLLYAEGETAPKMMSVGLHPRLAGRPGRARALARFLDYIAGHEKVWVARRVDIA
HHHCCHHEEEECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHH
RHWIATHPPVGGYKPTFMPRGLFVERFGGVFEHSPWIAEAAYDKGLTPREDTAAGLHAAL
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHH
AAVLRAAPIEKQMGVIHAHPDLAGKLAEAKLLTAESTAEQASAGLDRLTAEEKARFTAFN
HHHHHHCCCHHHCCEEECCCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHEEECC
DAYVAKFGFVFIMAIRGRTKAEILDAFERRLAHDRDQEVNEALTQIERIARLRLDQMLPE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AA
CC
>Mature Secondary Structure 
SVPNDPITGRPYPRDLIGYGRNPPHARWPGEARIAVQFVVNYEEGGENSILHGDNASEA
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEECCCCCHHH
FLSEIIGAQPWPRQRHMNMESIYEYGARVGFWRLHRLFTERAMPVTVYGVATALARNPDA
HHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCHH
VAAMKEADWEIASHGYKWLEYKDFTRDAEREHIEEAIRIHTEVTGSRPLGFYQGRSAEHT
HHHHHHCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VPLVMEEGGFLYAADSYADELPYWIESAKGPQLVVPYTLDANDMRFSVAAGFGTGTDFFD
EEEEEECCCEEEEECCHHHHCCHHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHH
YLKDSFDLLYAEGETAPKMMSVGLHPRLAGRPGRARALARFLDYIAGHEKVWVARRVDIA
HHHCCHHEEEECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHH
RHWIATHPPVGGYKPTFMPRGLFVERFGGVFEHSPWIAEAAYDKGLTPREDTAAGLHAAL
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHH
AAVLRAAPIEKQMGVIHAHPDLAGKLAEAKLLTAESTAEQASAGLDRLTAEEKARFTAFN
HHHHHHCCCHHHCCEEECCCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHEEECC
DAYVAKFGFVFIMAIRGRTKAEILDAFERRLAHDRDQEVNEALTQIERIARLRLDQMLPE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]