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The map label for this gene is fadE25 [H]

Identifier: 154247115

GI number: 154247115

Start: 3532793

End: 3534355

Strand: Reverse

Name: fadE25 [H]

Synonym: Xaut_3186

Alternate gene names: 154247115

Gene position: 3534355-3532793 (Counterclockwise)

Preceding gene: 154247117

Following gene: 154247114

Centisome position: 66.57

GC content: 64.43

Gene sequence:

>1563_bases
ATGACGCCTAACGAATGGGCTTGCACAAGCCGCAATCATCGGCACAGTGTTTCTACGGCGTCAAAAAAGTTCACTGAGGG
GAACAGACTGCCGCCTTCGCGAAGATCGCAACTGCGGGACGGCCCGGTCGGGGCGCCGGCCCAGGCGGAGCTTGAAATCA
TGTCCGATGTGAAGATGTGGGGCAAGGAAGAATTCTGGGGTCTCGGCTTCGAGTGGGATCCCCAATGGCTGCTCACGCCG
GCGCAGAAGGAACTGCAGAAGAAGCTCATCGCCCTGTGCGAGACCACGCTTCGCGCCAACGCCGTCGAGAGCGACGCCAA
CTACGTCTACCCCCGCAAGAATTTCGAGGCCCTCGCCTCCCTCGGCCTGCTCGCGCTGAACGTGCCGAAGGAGCTGGGCG
GACTGGGCGAGAATCACCTGTGCGCCGCCATGGTGGTGGAGACCATCGCCCGCTACGGCTGCCCCTCCACCGCCATGTGC
TACACCATGCACCTGGGTGCCACGGCGGCCGCGCTCCTGCGCCACCACGACAACCCCATCCTAACCGACATCCTCAAGCG
GCTGGACAAGGACGTGCTGATCGGCACCCTGTCCTATTCCGACCCCGAGACCGGCTCCCATTTCTGGTATCCCATCTCGT
CCAAGGCGGAAGCCTATGGCGACGGCTGGAAGGTGACCAAGAAGGCCTCCTGGACCACCTCCGGCGGCTTTGCCGACTGG
TACATCGTGCAGACTACCAGCCCCGGCTTTGCCGGGGACTATTCGGACCTGTCCTGCTTCCTCGTCACCAAGGACGAGGT
GAAGGCGAACCCCTCCGAATGGGATGGCCTGGGCCTGCGCGGCAACCAGTCCGGCCCCATCGTGGTGGAAAACGCCATCC
TGCCGAAGGACGCCCTGGTCGGCCCCATCGGCGACGGCGCCAAGTCCAACGACGAGGTGGTGGACCCGTTCTTCCTCACC
TGCTCGTCGGCCTGCTGGAACGGCATTTCGCTGGCGGTCATCGACATCGCCAAGCGCCATACCACCCGCAAGACCCACAA
TGACGTGGGCATGCGCGTCGCCGACTATCCCACCATCCAGGACTATGTGGGCGAGGCGATCATGGACACCAACGCCTGCC
GGGCGCTGGACTTCCAGATGGCGCAGGCGCTCGACAGCGTGACCAACAATTGCGACTGGTCCATCCACGCCGATGTGGAC
GCCCTGCCCCGCTCCGCCCTGCTGCACTGGCTGTGGCAGGTGAAGTTCGAGGCGGCCAAGAACGTGGCCCATGTGGTGGA
CAAGATGCTCCACGCCACAGGCGGCACCGGCTTCCGCCCGGCGCTCCAGATCGAGCGCTACCTGCGCGACGGCAAGGCCG
GCTGGGTGATGGGCCCCACCAACGAGGTGCTGCGCCAGTTCGTGGGCAAGGCCTCGCTGCTCGGCTTCGGCTCGCTGGAC
TACTGGAACAAGTCCATCAACGAGCGGGTGCTCAACAACGAGATCAAGAAGATGGACGACGCCGAGAAGCGTGCGCTGGC
CGAAAAGCTGCTGGCCGAGATCCAGCCCGCGAAGGTGGCATGA

Upstream 100 bases:

>100_bases
TTCCTGAGAAGAAATAATTTCTGCCCCATGCGCAATATCGAGGCAGGGCAAAGGCCCATCGCCGCTAAGCGCGGCCATAT
CCGGCTGAAAATTGAGCGCC

Downstream 100 bases:

>100_bases
ACGAGAGGGCCTGAAAAAGCGGGAGGGCGGCACGCGCTCGCCCCTCCCGCACCCGTCCTGCGGCTTTGCGCCATCCCTCC
GAGCGCCCGCCATCAAGAGG

Product: acyl-CoA dehydrogenase domain-containing protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTPNEWACTSRNHRHSVSTASKKFTEGNRLPPSRRSQLRDGPVGAPAQAELEIMSDVKMWGKEEFWGLGFEWDPQWLLTP
AQKELQKKLIALCETTLRANAVESDANYVYPRKNFEALASLGLLALNVPKELGGLGENHLCAAMVVETIARYGCPSTAMC
YTMHLGATAAALLRHHDNPILTDILKRLDKDVLIGTLSYSDPETGSHFWYPISSKAEAYGDGWKVTKKASWTTSGGFADW
YIVQTTSPGFAGDYSDLSCFLVTKDEVKANPSEWDGLGLRGNQSGPIVVENAILPKDALVGPIGDGAKSNDEVVDPFFLT
CSSACWNGISLAVIDIAKRHTTRKTHNDVGMRVADYPTIQDYVGEAIMDTNACRALDFQMAQALDSVTNNCDWSIHADVD
ALPRSALLHWLWQVKFEAAKNVAHVVDKMLHATGGTGFRPALQIERYLRDGKAGWVMGPTNEVLRQFVGKASLLGFGSLD
YWNKSINERVLNNEIKKMDDAEKRALAEKLLAEIQPAKVA

Sequences:

>Translated_520_residues
MTPNEWACTSRNHRHSVSTASKKFTEGNRLPPSRRSQLRDGPVGAPAQAELEIMSDVKMWGKEEFWGLGFEWDPQWLLTP
AQKELQKKLIALCETTLRANAVESDANYVYPRKNFEALASLGLLALNVPKELGGLGENHLCAAMVVETIARYGCPSTAMC
YTMHLGATAAALLRHHDNPILTDILKRLDKDVLIGTLSYSDPETGSHFWYPISSKAEAYGDGWKVTKKASWTTSGGFADW
YIVQTTSPGFAGDYSDLSCFLVTKDEVKANPSEWDGLGLRGNQSGPIVVENAILPKDALVGPIGDGAKSNDEVVDPFFLT
CSSACWNGISLAVIDIAKRHTTRKTHNDVGMRVADYPTIQDYVGEAIMDTNACRALDFQMAQALDSVTNNCDWSIHADVD
ALPRSALLHWLWQVKFEAAKNVAHVVDKMLHATGGTGFRPALQIERYLRDGKAGWVMGPTNEVLRQFVGKASLLGFGSLD
YWNKSINERVLNNEIKKMDDAEKRALAEKLLAEIQPAKVA
>Mature_519_residues
TPNEWACTSRNHRHSVSTASKKFTEGNRLPPSRRSQLRDGPVGAPAQAELEIMSDVKMWGKEEFWGLGFEWDPQWLLTPA
QKELQKKLIALCETTLRANAVESDANYVYPRKNFEALASLGLLALNVPKELGGLGENHLCAAMVVETIARYGCPSTAMCY
TMHLGATAAALLRHHDNPILTDILKRLDKDVLIGTLSYSDPETGSHFWYPISSKAEAYGDGWKVTKKASWTTSGGFADWY
IVQTTSPGFAGDYSDLSCFLVTKDEVKANPSEWDGLGLRGNQSGPIVVENAILPKDALVGPIGDGAKSNDEVVDPFFLTC
SSACWNGISLAVIDIAKRHTTRKTHNDVGMRVADYPTIQDYVGEAIMDTNACRALDFQMAQALDSVTNNCDWSIHADVDA
LPRSALLHWLWQVKFEAAKNVAHVVDKMLHATGGTGFRPALQIERYLRDGKAGWVMGPTNEVLRQFVGKASLLGFGSLDY
WNKSINERVLNNEIKKMDDAEKRALAEKLLAEIQPAKVA

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=390, Percent_Identity=24.8717948717949, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI21361497, Length=369, Percent_Identity=24.1192411924119, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI226958412, Length=400, Percent_Identity=24.25, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI226958414, Length=365, Percent_Identity=24.9315068493151, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4557231, Length=415, Percent_Identity=23.855421686747, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI187960098, Length=415, Percent_Identity=23.855421686747, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI76496475, Length=373, Percent_Identity=20.6434316353887, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4557235, Length=373, Percent_Identity=20.6434316353887, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI4501859, Length=386, Percent_Identity=20.4663212435233, Blast_Score=76, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71985184, Length=400, Percent_Identity=23.25, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17508101, Length=351, Percent_Identity=21.9373219373219, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17538396, Length=378, Percent_Identity=24.0740740740741, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17534899, Length=410, Percent_Identity=22.1951219512195, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17569725, Length=402, Percent_Identity=21.8905472636816, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17570075, Length=402, Percent_Identity=21.3930348258706, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17551932, Length=395, Percent_Identity=22.0253164556962, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71985192, Length=346, Percent_Identity=22.2543352601156, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17506239, Length=358, Percent_Identity=21.5083798882682, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17505929, Length=224, Percent_Identity=24.1071428571429, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356377, Length=403, Percent_Identity=23.5732009925558, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281363737, Length=407, Percent_Identity=22.3587223587224, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24660351, Length=408, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355753, Length=406, Percent_Identity=23.1527093596059, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24646207, Length=404, Percent_Identity=21.5346534653465, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 57303; Mature: 57171

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNEWACTSRNHRHSVSTASKKFTEGNRLPPSRRSQLRDGPVGAPAQAELEIMSDVKMW
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHC
GKEEFWGLGFEWDPQWLLTPAQKELQKKLIALCETTLRANAVESDANYVYPRKNFEALAS
CCHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHH
LGLLALNVPKELGGLGENHLCAAMVVETIARYGCPSTAMCYTMHLGATAAALLRHHDNPI
HHHHEEECCHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHHHCCCHH
LTDILKRLDKDVLIGTLSYSDPETGSHFWYPISSKAEAYGDGWKVTKKASWTTSGGFADW
HHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCHHCCCCEEEEECCCCCCCCCCEEE
YIVQTTSPGFAGDYSDLSCFLVTKDEVKANPSEWDGLGLRGNQSGPIVVENAILPKDALV
EEEEECCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHCC
GPIGDGAKSNDEVVDPFFLTCSSACWNGISLAVIDIAKRHTTRKTHNDVGMRVADYPTIQ
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCEECCCCCHH
DYVGEAIMDTNACRALDFQMAQALDSVTNNCDWSIHADVDALPRSALLHWLWQVKFEAAK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHH
NVAHVVDKMLHATGGTGFRPALQIERYLRDGKAGWVMGPTNEVLRQFVGKASLLGFGSLD
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCHHHCCCCCHH
YWNKSINERVLNNEIKKMDDAEKRALAEKLLAEIQPAKVA
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TPNEWACTSRNHRHSVSTASKKFTEGNRLPPSRRSQLRDGPVGAPAQAELEIMSDVKMW
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHC
GKEEFWGLGFEWDPQWLLTPAQKELQKKLIALCETTLRANAVESDANYVYPRKNFEALAS
CCHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHH
LGLLALNVPKELGGLGENHLCAAMVVETIARYGCPSTAMCYTMHLGATAAALLRHHDNPI
HHHHEEECCHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHHHCCCHH
LTDILKRLDKDVLIGTLSYSDPETGSHFWYPISSKAEAYGDGWKVTKKASWTTSGGFADW
HHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCHHCCCCEEEEECCCCCCCCCCEEE
YIVQTTSPGFAGDYSDLSCFLVTKDEVKANPSEWDGLGLRGNQSGPIVVENAILPKDALV
EEEEECCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHCC
GPIGDGAKSNDEVVDPFFLTCSSACWNGISLAVIDIAKRHTTRKTHNDVGMRVADYPTIQ
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCEECCCCCHH
DYVGEAIMDTNACRALDFQMAQALDSVTNNCDWSIHADVDALPRSALLHWLWQVKFEAAK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHH
NVAHVVDKMLHATGGTGFRPALQIERYLRDGKAGWVMGPTNEVLRQFVGKASLLGFGSLD
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCHHHCCCCCHH
YWNKSINERVLNNEIKKMDDAEKRALAEKLLAEIQPAKVA
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]