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The map label for this gene is ntaB [H]

Identifier: 154247114

GI number: 154247114

Start: 3532137

End: 3532685

Strand: Reverse

Name: ntaB [H]

Synonym: Xaut_3185

Alternate gene names: 154247114

Gene position: 3532685-3532137 (Counterclockwise)

Preceding gene: 154247115

Following gene: 154247113

Centisome position: 66.54

GC content: 63.21

Gene sequence:

>549_bases
ATGGACGCAGTGACCCATTTCAGAGAGTTCCGTTCGATGACTGCCATTGATCTCGACGCCCGTCTCGATCCGCGTGAATT
GCGCAATGCCCTCGGCCGCTTCGCCACCGGCATCGCCGTGGTGATGGCGAAGGATTCCGAAGGGCCCATGGGCGTCACGG
TGAATTCCTTTTCCGCCGTTTCGCTGGATCCGCCGCTGATCCTGTTCTGCATGGCGCGCTCGCTCCATTCGCTGGAGCGG
CTGGAGAAGGCAAAGGCCTACTCGGTCAACATCCTGCTGGAGCACCAGAAGGAAATCTCCAACCGCTTCGCCCGCGCCGG
CGAGGACAAGTTCGCGGACACGCCCTGCGCCGACGGGCGGCTCGGCGAGCCGCGGCTGGAATCCGCCCATGCGGTCTTCG
AGTGCGTGCCCTATGCCCATTATGACGGGGGCGACCATGTGATCTTCGTCGGCCGGGTGGTGCACATGCACACCGACGGC
GAGGGCGATCCTCTTCTTTATTACCGCGGCAGTTACCGGCTGCTCGCGAGCCTCATGCAAGACAGCTGA

Upstream 100 bases:

>100_bases
GGCCTGAAAAAGCGGGAGGGCGGCACGCGCTCGCCCCTCCCGCACCCGTCCTGCGGCTTTGCGCCATCCCTCCGAGCGCC
CGCCATCAAGAGGGATTATC

Downstream 100 bases:

>100_bases
ACCAGAGGGCTGAATGGACGCTTCCCTCCTCCCGCCCGGCCCGTCGCGCAGCGCGCCCGCCACCCGCCCCCGCGAATGCG
TGGTGGTGCGCGGCGGCGGT

Product: flavin reductase domain-containing protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MDAVTHFREFRSMTAIDLDARLDPRELRNALGRFATGIAVVMAKDSEGPMGVTVNSFSAVSLDPPLILFCMARSLHSLER
LEKAKAYSVNILLEHQKEISNRFARAGEDKFADTPCADGRLGEPRLESAHAVFECVPYAHYDGGDHVIFVGRVVHMHTDG
EGDPLLYYRGSYRLLASLMQDS

Sequences:

>Translated_182_residues
MDAVTHFREFRSMTAIDLDARLDPRELRNALGRFATGIAVVMAKDSEGPMGVTVNSFSAVSLDPPLILFCMARSLHSLER
LEKAKAYSVNILLEHQKEISNRFARAGEDKFADTPCADGRLGEPRLESAHAVFECVPYAHYDGGDHVIFVGRVVHMHTDG
EGDPLLYYRGSYRLLASLMQDS
>Mature_182_residues
MDAVTHFREFRSMTAIDLDARLDPRELRNALGRFATGIAVVMAKDSEGPMGVTVNSFSAVSLDPPLILFCMARSLHSLER
LEKAKAYSVNILLEHQKEISNRFARAGEDKFADTPCADGRLGEPRLESAHAVFECVPYAHYDGGDHVIFVGRVVHMHTDG
EGDPLLYYRGSYRLLASLMQDS

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 20229; Mature: 20229

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVTHFREFRSMTAIDLDARLDPRELRNALGRFATGIAVVMAKDSEGPMGVTVNSFSAV
CCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCEEEECCCEEE
SLDPPLILFCMARSLHSLERLEKAKAYSVNILLEHQKEISNRFARAGEDKFADTPCADGR
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCC
LGEPRLESAHAVFECVPYAHYDGGDHVIFVGRVVHMHTDGEGDPLLYYRGSYRLLASLMQ
CCCCHHHHHHHHHHHCCCCEECCCCEEEEEEEEEEEEECCCCCEEEEECCCHHHHHHHHH
DS
CC
>Mature Secondary Structure
MDAVTHFREFRSMTAIDLDARLDPRELRNALGRFATGIAVVMAKDSEGPMGVTVNSFSAV
CCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCEEEECCCEEE
SLDPPLILFCMARSLHSLERLEKAKAYSVNILLEHQKEISNRFARAGEDKFADTPCADGR
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCC
LGEPRLESAHAVFECVPYAHYDGGDHVIFVGRVVHMHTDGEGDPLLYYRGSYRLLASLMQ
CCCCHHHHHHHHHHHCCCCEECCCCEEEEEEEEEEEEECCCCCEEEEECCCHHHHHHHHH
DS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]