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The map label for this gene is mepA

Identifier: 154247027

GI number: 154247027

Start: 3438965

End: 3439894

Strand: Reverse

Name: mepA

Synonym: Xaut_3098

Alternate gene names: 154247027

Gene position: 3439894-3438965 (Counterclockwise)

Preceding gene: 154247028

Following gene: 154247026

Centisome position: 64.79

GC content: 72.37

Gene sequence:

>930_bases
ATGCGCCGCGCCGTCGGCGCCCTGCTCGGCCTGCTGGTCGCGGGCGCGCCGGCGCTGGTGCTGGCGCAGCAGGTAGCGCC
CCCGGCCGCGCTTCCCGGCAAGCCGGCGGCGCGGCCCGCCGCGGCAGGGCCGGTCCCGGCTAAGGAGCTGTTCGGACGGG
TGCAGGACCCCGCCCCCCTCGCCGCCCGCTCCATCGGCTTTTATTCCAAGGGCTGTCTCGCGGGCGCGCAGGCGCTGGCG
GTGAACGGGCCGAACTGGCAGGTCATGCGGCTGTCCCGCAACCGCAATTTCGGCCATCCCGATCTCGTCGTCTTCCTGGA
GCGCTTCTCCGCGCAGGTGCCGAAGGTGTCGTCCTGGCAGGGCATCCTTGTGGGCGACATGTCCCAGCCGCGCGGCGGCC
CCATGCTCACCGGCCACGCCTCCCACCAGATCGGCCTCGACGCCGACATCTGGCTGACCCCGAGCCCCGGCCGGGAGCTG
AGCCGGGAGGAGCGCGAGAAGCTGTCCGCGTCCATGATGGTGCGCGCCGACCGCCGCGACATCGATCCCGCCAACTGGCG
GCCCGACCACTGGAAGGTCATCCGCGCCGCCGCCCTCGACCCGCGCGTGGAACGCATCTTCGTGAACGCGGCCATCAAGA
AGGCCCTGTGCCGGGAGGCGAGCGGCGACCGCTCCTTCCTCGCCAAGGTGCGTCCCTACTGGGGCCACGACTACCACATG
CATGTCCGCCTCACCTGCCCGCCGGGCAACCCGGACTGCCGCCCACAGGAAGCCCCACCCGCCGGCGAGGGCTGCGGATC
GGAACTGGACTGGTGGTTCACCGACGAAGTGCTGCACCCCAAGCCCGAGAAGGAACCGGCGAAGCCCAAGCCGCCTTTAA
CCCTTGCCGACCTGCCCGAGGCCTGCGCGGCGGTGCTGGGCGCCAGATGA

Upstream 100 bases:

>100_bases
TGACGCCGGGGGCGACCCTGTTCGTCACCGACCTGCCGGCCCATCCGGGCACCCGCAACGACGGCGGCTTCGTGGTGATG
ACCCACAAGGTGGAAAGCTG

Downstream 100 bases:

>100_bases
AGAACCGCATGAGGGCGTGAACCCGCCCGGCGTGGGGAAGCAACGATAACGGAGGACGCCATGACCGACCTTACCGACCT
CTTCCCCGGCTTCGACTCTC

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRRAVGALLGLLVAGAPALVLAQQVAPPAALPGKPAARPAAAGPVPAKELFGRVQDPAPLAARSIGFYSKGCLAGAQALA
VNGPNWQVMRLSRNRNFGHPDLVVFLERFSAQVPKVSSWQGILVGDMSQPRGGPMLTGHASHQIGLDADIWLTPSPGREL
SREEREKLSASMMVRADRRDIDPANWRPDHWKVIRAAALDPRVERIFVNAAIKKALCREASGDRSFLAKVRPYWGHDYHM
HVRLTCPPGNPDCRPQEAPPAGEGCGSELDWWFTDEVLHPKPEKEPAKPKPPLTLADLPEACAAVLGAR

Sequences:

>Translated_309_residues
MRRAVGALLGLLVAGAPALVLAQQVAPPAALPGKPAARPAAAGPVPAKELFGRVQDPAPLAARSIGFYSKGCLAGAQALA
VNGPNWQVMRLSRNRNFGHPDLVVFLERFSAQVPKVSSWQGILVGDMSQPRGGPMLTGHASHQIGLDADIWLTPSPGREL
SREEREKLSASMMVRADRRDIDPANWRPDHWKVIRAAALDPRVERIFVNAAIKKALCREASGDRSFLAKVRPYWGHDYHM
HVRLTCPPGNPDCRPQEAPPAGEGCGSELDWWFTDEVLHPKPEKEPAKPKPPLTLADLPEACAAVLGAR
>Mature_309_residues
MRRAVGALLGLLVAGAPALVLAQQVAPPAALPGKPAARPAAAGPVPAKELFGRVQDPAPLAARSIGFYSKGCLAGAQALA
VNGPNWQVMRLSRNRNFGHPDLVVFLERFSAQVPKVSSWQGILVGDMSQPRGGPMLTGHASHQIGLDADIWLTPSPGREL
SREEREKLSASMMVRADRRDIDPANWRPDHWKVIRAAALDPRVERIFVNAAIKKALCREASGDRSFLAKVRPYWGHDYHM
HVRLTCPPGNPDCRPQEAPPAGEGCGSELDWWFTDEVLHPKPEKEPAKPKPPLTLADLPEACAAVLGAR

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=255, Percent_Identity=43.1372549019608, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 33444; Mature: 33444

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAVGALLGLLVAGAPALVLAQQVAPPAALPGKPAARPAAAGPVPAKELFGRVQDPAPL
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCH
AARSIGFYSKGCLAGAQALAVNGPNWQVMRLSRNRNFGHPDLVVFLERFSAQVPKVSSWQ
HHHHCCCCCCCHHCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
GILVGDMSQPRGGPMLTGHASHQIGLDADIWLTPSPGRELSREEREKLSASMMVRADRRD
EEEEECCCCCCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHEECCCCC
IDPANWRPDHWKVIRAAALDPRVERIFVNAAIKKALCREASGDRSFLAKVRPYWGHDYHM
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEE
HVRLTCPPGNPDCRPQEAPPAGEGCGSELDWWFTDEVLHPKPEKEPAKPKPPLTLADLPE
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEHHHHHH
ACAAVLGAR
HHHHHHCCC
>Mature Secondary Structure
MRRAVGALLGLLVAGAPALVLAQQVAPPAALPGKPAARPAAAGPVPAKELFGRVQDPAPL
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCH
AARSIGFYSKGCLAGAQALAVNGPNWQVMRLSRNRNFGHPDLVVFLERFSAQVPKVSSWQ
HHHHCCCCCCCHHCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
GILVGDMSQPRGGPMLTGHASHQIGLDADIWLTPSPGRELSREEREKLSASMMVRADRRD
EEEEECCCCCCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHEECCCCC
IDPANWRPDHWKVIRAAALDPRVERIFVNAAIKKALCREASGDRSFLAKVRPYWGHDYHM
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEE
HVRLTCPPGNPDCRPQEAPPAGEGCGSELDWWFTDEVLHPKPEKEPAKPKPPLTLADLPE
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEHHHHHH
ACAAVLGAR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA