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The map label for this gene is tolB [H]

Identifier: 154246985

GI number: 154246985

Start: 3395637

End: 3396989

Strand: Reverse

Name: tolB [H]

Synonym: Xaut_3056

Alternate gene names: 154246985

Gene position: 3396989-3395637 (Counterclockwise)

Preceding gene: 154246986

Following gene: 154246984

Centisome position: 63.99

GC content: 68.51

Gene sequence:

>1353_bases
ATGAACGGGTTCGGACGGCCGGCCATGCTCGGGATGGGACGCCGGGCATTCATCAAGACCGGACTTGCCGCCGCGAGCCT
CGCCGGCCTCGGTGCGGGGCTGGGCACTGGCCCGGCGGGTGCCGCGCAGCCGCGCATCGACCTCACCCCCGGTGCCTTCA
AGCCCATCCCAATCGCCATCACCGACCTGCTCGGCGAGGTCGAGATTGGCCGCAACATTTCCGGCGTCATCACCGCCGAC
CTGCGGCGCTCGGGCCTGTTCGCGCCCATCGATCCCAAGGCGTTCGTGGAGCAGATCACCAATCCCGATACGCCGCGCTT
CCAGGACTGGCGCGTCATCAATGCCCAGGCGCTGCTCACCGGCCGGGTCACCCGGCAGGCGGACGGGCGCTTCCAGGTGG
TGTTCCGCCTGTGGGACGTGTTCGCCGGCCAGCAGCTCGCCGCCCAGCAGTTCGTCGCCCAGCCCGACAACTGGCGCCGC
ATCGCCCACATCATCGCCGACGCCATCTATGAGAAGATGACCGGCGAAAAGGGCTATTTCGACACCCGCATCGTGTTCGT
GGACGAGAGCGGCCCGAAGGAGCGGCGGGTCAAGCGCCTCGCCATCATGGACCAGGACGGCGCCAACGTGAGCTATCTGT
CGCGCGGCGACGGCCTCGTGCTCACCCCGCGCTTCTCGCCCACCAGCCAGGAAATCACCTACATGTCCTATGGGGCGGGT
GACCCCAAGGTGTTCCTTCTGAACATCGAGACCGGCCAGCGCGAGATCGTCGGCAACTTCCCGGGCATGAGCTTCTCGCC
ACGCTTCGCCCCGGACGGGCAGCGGGTGATCATGAGCCTGCAGCAGGGCGGCAATTCCAACATCTTCGTCATGGACCTGC
GCTCGCGCGCCACCACCCGCCTCACCGACACCGCCGCCATCGACACCGCCCCCTGCTATTCGCCGGACGGCCGGCAGATC
TGCTTCGAGAGCGATCGCGGCGGCACCTCGCAGATCTATGTGATGAATGCCGACGGCTCCGGCGCGCGGCGCATCTCGTT
CGGCAATGCGCGCTACTCGACCCCGGTGTGGTCGCCCAAGGGCGACTGGATCGCCTACACCCGCCAGGCCGAAGGCCGCT
TCGCCATCGGCGTGATGCGCACCGACGGCTCGGGCGAGCGCGTGCTCACCGAGGGCTACCACAACGAGGGGCCGACGTTC
GCGCCCAACGGCCGCGTCATCATGTTCTTCCGCGATCCCGGCGGCGCCTCCGGGCCGTCGCTCTATTCGGTGGACGTGAC
CGGCTACAACGAGCAGCGCGTGCCCACCCCGAGCTATGCCTCTGACCCCGCCTGGTCGCCCCTGCGCTCCTGA

Upstream 100 bases:

>100_bases
ACAAGCTGTTCAGCCGCTGATGCCGCGTCCGGGCGAAGCCCTCCGGCTTCTGATCCAGATGATGGCGCGCGGCGCAGCGC
ATGGAAAACGAGGGATATAA

Downstream 100 bases:

>100_bases
GGCCGGCGGCCTGCGCCCTTCCGCAGCCTCTCCCGCCTCGGAGAGTGGAGGGCATTGGGGACGAACGCCCGCCGGCGGGA
TGGCCAAAGCCGCCTGAGCC

Product: beta-propeller repeat-containing to-pal system protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNGFGRPAMLGMGRRAFIKTGLAAASLAGLGAGLGTGPAGAAQPRIDLTPGAFKPIPIAITDLLGEVEIGRNISGVITAD
LRRSGLFAPIDPKAFVEQITNPDTPRFQDWRVINAQALLTGRVTRQADGRFQVVFRLWDVFAGQQLAAQQFVAQPDNWRR
IAHIIADAIYEKMTGEKGYFDTRIVFVDESGPKERRVKRLAIMDQDGANVSYLSRGDGLVLTPRFSPTSQEITYMSYGAG
DPKVFLLNIETGQREIVGNFPGMSFSPRFAPDGQRVIMSLQQGGNSNIFVMDLRSRATTRLTDTAAIDTAPCYSPDGRQI
CFESDRGGTSQIYVMNADGSGARRISFGNARYSTPVWSPKGDWIAYTRQAEGRFAIGVMRTDGSGERVLTEGYHNEGPTF
APNGRVIMFFRDPGGASGPSLYSVDVTGYNEQRVPTPSYASDPAWSPLRS

Sequences:

>Translated_450_residues
MNGFGRPAMLGMGRRAFIKTGLAAASLAGLGAGLGTGPAGAAQPRIDLTPGAFKPIPIAITDLLGEVEIGRNISGVITAD
LRRSGLFAPIDPKAFVEQITNPDTPRFQDWRVINAQALLTGRVTRQADGRFQVVFRLWDVFAGQQLAAQQFVAQPDNWRR
IAHIIADAIYEKMTGEKGYFDTRIVFVDESGPKERRVKRLAIMDQDGANVSYLSRGDGLVLTPRFSPTSQEITYMSYGAG
DPKVFLLNIETGQREIVGNFPGMSFSPRFAPDGQRVIMSLQQGGNSNIFVMDLRSRATTRLTDTAAIDTAPCYSPDGRQI
CFESDRGGTSQIYVMNADGSGARRISFGNARYSTPVWSPKGDWIAYTRQAEGRFAIGVMRTDGSGERVLTEGYHNEGPTF
APNGRVIMFFRDPGGASGPSLYSVDVTGYNEQRVPTPSYASDPAWSPLRS
>Mature_450_residues
MNGFGRPAMLGMGRRAFIKTGLAAASLAGLGAGLGTGPAGAAQPRIDLTPGAFKPIPIAITDLLGEVEIGRNISGVITAD
LRRSGLFAPIDPKAFVEQITNPDTPRFQDWRVINAQALLTGRVTRQADGRFQVVFRLWDVFAGQQLAAQQFVAQPDNWRR
IAHIIADAIYEKMTGEKGYFDTRIVFVDESGPKERRVKRLAIMDQDGANVSYLSRGDGLVLTPRFSPTSQEITYMSYGAG
DPKVFLLNIETGQREIVGNFPGMSFSPRFAPDGQRVIMSLQQGGNSNIFVMDLRSRATTRLTDTAAIDTAPCYSPDGRQI
CFESDRGGTSQIYVMNADGSGARRISFGNARYSTPVWSPKGDWIAYTRQAEGRFAIGVMRTDGSGERVLTEGYHNEGPTF
APNGRVIMFFRDPGGASGPSLYSVDVTGYNEQRVPTPSYASDPAWSPLRS

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=401, Percent_Identity=28.927680798005, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR006311
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 48973; Mature: 48973

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFGRPAMLGMGRRAFIKTGLAAASLAGLGAGLGTGPAGAAQPRIDLTPGAFKPIPIAI
CCCCCCCHHHCCCCHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEH
TDLLGEVEIGRNISGVITADLRRSGLFAPIDPKAFVEQITNPDTPRFQDWRVINAQALLT
HHHHCCHHCCCCCCEEEEECHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEEE
GRVTRQADGRFQVVFRLWDVFAGQQLAAQQFVAQPDNWRRIAHIIADAIYEKMTGEKGYF
ECEEECCCCCEEEEEEHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCE
DTRIVFVDESGPKERRVKRLAIMDQDGANVSYLSRGDGLVLTPRFSPTSQEITYMSYGAG
EEEEEEEECCCCHHHHEEEEEEECCCCCCEEEEECCCCEEEECCCCCCCCEEEEEEECCC
DPKVFLLNIETGQREIVGNFPGMSFSPRFAPDGQRVIMSLQQGGNSNIFVMDLRSRATTR
CCEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHEECCCCCCCEEEEECCCCCCEE
LTDTAAIDTAPCYSPDGRQICFESDRGGTSQIYVMNADGSGARRISFGNARYSTPVWSPK
EECCCEECCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEECCCEECCCCCCCC
GDWIAYTRQAEGRFAIGVMRTDGSGERVLTEGYHNEGPTFAPNGRVIMFFRDPGGASGPS
CCEEEEEEECCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCE
LYSVDVTGYNEQRVPTPSYASDPAWSPLRS
EEEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGFGRPAMLGMGRRAFIKTGLAAASLAGLGAGLGTGPAGAAQPRIDLTPGAFKPIPIAI
CCCCCCCHHHCCCCHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEH
TDLLGEVEIGRNISGVITADLRRSGLFAPIDPKAFVEQITNPDTPRFQDWRVINAQALLT
HHHHCCHHCCCCCCEEEEECHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEEE
GRVTRQADGRFQVVFRLWDVFAGQQLAAQQFVAQPDNWRRIAHIIADAIYEKMTGEKGYF
ECEEECCCCCEEEEEEHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCE
DTRIVFVDESGPKERRVKRLAIMDQDGANVSYLSRGDGLVLTPRFSPTSQEITYMSYGAG
EEEEEEEECCCCHHHHEEEEEEECCCCCCEEEEECCCCEEEECCCCCCCCEEEEEEECCC
DPKVFLLNIETGQREIVGNFPGMSFSPRFAPDGQRVIMSLQQGGNSNIFVMDLRSRATTR
CCEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHEECCCCCCCEEEEECCCCCCEE
LTDTAAIDTAPCYSPDGRQICFESDRGGTSQIYVMNADGSGARRISFGNARYSTPVWSPK
EECCCEECCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEECCCEECCCCCCCC
GDWIAYTRQAEGRFAIGVMRTDGSGERVLTEGYHNEGPTFAPNGRVIMFFRDPGGASGPS
CCEEEEEEECCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCE
LYSVDVTGYNEQRVPTPSYASDPAWSPLRS
EEEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]