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The map label for this gene is gsiB [H]

Identifier: 154246699

GI number: 154246699

Start: 3056144

End: 3057778

Strand: Direct

Name: gsiB [H]

Synonym: Xaut_2760

Alternate gene names: 154246699

Gene position: 3056144-3057778 (Clockwise)

Preceding gene: 154246697

Following gene: 154246700

Centisome position: 57.57

GC content: 65.32

Gene sequence:

>1635_bases
ATGGGTCATCTGGCGCGATGGAAAGCGGGTGCTGTGCTCGCGGTGGCGGCAGTTCTCGTGGCGGGGGCCGCCGGCGCTCA
GCAGGCCGGGGACACGCCGAAGCGCGGCGGCACGCTGTCTGCCATCATCCAGCCCGAGCCCGTCATTCTCACCGCCGCGC
TCAATACGGCGGCGCCCACCGGCATCGTCACCGGCTCCATCTTCGACGGCCTCGTGGACTATGATTCCAACCTCAGGCCG
GTGGCGGCCCTGGCCGAGAGCTGGGAAACTGCGAAGGACGGCCTCACCTTCACCCTGCATCTGCGGGGCGGGGTGAAGTG
GCACGACGGCAAGCCCTTCACCTCCGCCGACGTGAAGTGGACGCTGGAGGAGGTGTGGAAGAAGATCCACCCCCGCAACC
AGGCCATCTTCGCCAAGGTCACCTCGGTGGACACGCCGGACGACCGCACCGTGGTGCTGCACCTGAGCCAGCCCTCGGTG
GCGATCCTGTCCTCGCTGAATTCCAACGGCGCCCAGGTGCTGCCCAAGCATCTCTACGAGGGCACCGACATCCTCAACAA
TCCCTATAACAACAAGCCGGTGGGTACCGGGCCGTTCGTCTTCAAGGAATGGAGCCGCGGGAATTACATCGTGCTTGAGC
GCAATCCCGAATACTGGGACAAGGGCAAGCCTTACCTCGACCGCGTGGTGTTCCGTGTGATCCCCGACGCCGCCGCGCGC
TCCGCAGCGCTGGAGAAGGGCGAAGTGCAGTGGGGCGTGCTGAGCCCGGTGCCGCTGAAGGATGCCGAGCGCCTTGTCAG
GCTGCCCAACCTTCGCGTTTCCACCGAAGGTTATGAGTGGCTCTCGCCCTGGCTGTTCGCCGACTTCAACGTGGAGCGCG
GCCCCCTCAGGGACGTGAAGGTGCGCCAGGCGCTGGCCCAGGCGGTCGACCGCAACGCCATCGCCAAGGTGGTGTGGTTC
GGCTTCGCCAAGCCCGCGACCAGCCCCATCCCCTCTACTCTGGCCGCGTTCCACGATGCCGGCGGCATCGCCTATGACTA
CGACCCCAGGAAGGCCGAGGCGCTGCTGGATGCGGCTGGTTACAAGCGCGGCCCGGACGGCACCCGCTTCACCCTCGATA
TCGACTACATTCCCTATGGCGACGACTTCAAGCGTACCGCCGAATACCTGAAGCAGGCGCTGAAGCGGGTGGGCGTCGAG
GCCAATGTCCGCTCGCAGGACACCGCCGCCTTTACCCGCCGCGTCTATGGCGATCGCGACTTCGACATCTCCATCACCTG
GTTCGCGGCCTTCTCCGATCCGCAGGTGGGCGTGAACCGCGCCTACTGGTCCGGCTCCGTCGGCAAGAACATTCCCTGGA
CCAACGGCTCGGGCTATCGCAACGACAAGGTGGACGCGCTGATTTCCGCCACCCAGGGCGAGGCCGATCCGGAAAAGCGC
ATCCAGCTGTTCAAGGACCTGCAGAAGACCGTTCTGACCGATCTTCCCACCCTTCCGCTGGTGGAGCTGAAGTTCTTCAC
CGTGGAGGCCGCCAATCTCAGGCATCCCAAGGTCCGCGGCGACCAGGTGTACGGCTCGTTCCGCGACTTCTGGATCGATG
ACGCAAAGGTAGCCGACGCGCCGAAATCCAACTGA

Upstream 100 bases:

>100_bases
ATACATAGTATATTAAATCAGTAGAAAATATTGACTAACAGGGCGTCGGCGCATATCGTCTCAAATAGCGAGTTTGAGAT
GTATTATGTGGAGTCCGAAA

Downstream 100 bases:

>100_bases
GTTTCTCCCGGAACTTGGCCGTCCCGGTTCGCCCGGACGGCCCCTTCTTTCATGTCCGAGACACCGCCATGGCTTCCTTT
TCCCGCCGCCAATTCCTTGC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MGHLARWKAGAVLAVAAVLVAGAAGAQQAGDTPKRGGTLSAIIQPEPVILTAALNTAAPTGIVTGSIFDGLVDYDSNLRP
VAALAESWETAKDGLTFTLHLRGGVKWHDGKPFTSADVKWTLEEVWKKIHPRNQAIFAKVTSVDTPDDRTVVLHLSQPSV
AILSSLNSNGAQVLPKHLYEGTDILNNPYNNKPVGTGPFVFKEWSRGNYIVLERNPEYWDKGKPYLDRVVFRVIPDAAAR
SAALEKGEVQWGVLSPVPLKDAERLVRLPNLRVSTEGYEWLSPWLFADFNVERGPLRDVKVRQALAQAVDRNAIAKVVWF
GFAKPATSPIPSTLAAFHDAGGIAYDYDPRKAEALLDAAGYKRGPDGTRFTLDIDYIPYGDDFKRTAEYLKQALKRVGVE
ANVRSQDTAAFTRRVYGDRDFDISITWFAAFSDPQVGVNRAYWSGSVGKNIPWTNGSGYRNDKVDALISATQGEADPEKR
IQLFKDLQKTVLTDLPTLPLVELKFFTVEAANLRHPKVRGDQVYGSFRDFWIDDAKVADAPKSN

Sequences:

>Translated_544_residues
MGHLARWKAGAVLAVAAVLVAGAAGAQQAGDTPKRGGTLSAIIQPEPVILTAALNTAAPTGIVTGSIFDGLVDYDSNLRP
VAALAESWETAKDGLTFTLHLRGGVKWHDGKPFTSADVKWTLEEVWKKIHPRNQAIFAKVTSVDTPDDRTVVLHLSQPSV
AILSSLNSNGAQVLPKHLYEGTDILNNPYNNKPVGTGPFVFKEWSRGNYIVLERNPEYWDKGKPYLDRVVFRVIPDAAAR
SAALEKGEVQWGVLSPVPLKDAERLVRLPNLRVSTEGYEWLSPWLFADFNVERGPLRDVKVRQALAQAVDRNAIAKVVWF
GFAKPATSPIPSTLAAFHDAGGIAYDYDPRKAEALLDAAGYKRGPDGTRFTLDIDYIPYGDDFKRTAEYLKQALKRVGVE
ANVRSQDTAAFTRRVYGDRDFDISITWFAAFSDPQVGVNRAYWSGSVGKNIPWTNGSGYRNDKVDALISATQGEADPEKR
IQLFKDLQKTVLTDLPTLPLVELKFFTVEAANLRHPKVRGDQVYGSFRDFWIDDAKVADAPKSN
>Mature_543_residues
GHLARWKAGAVLAVAAVLVAGAAGAQQAGDTPKRGGTLSAIIQPEPVILTAALNTAAPTGIVTGSIFDGLVDYDSNLRPV
AALAESWETAKDGLTFTLHLRGGVKWHDGKPFTSADVKWTLEEVWKKIHPRNQAIFAKVTSVDTPDDRTVVLHLSQPSVA
ILSSLNSNGAQVLPKHLYEGTDILNNPYNNKPVGTGPFVFKEWSRGNYIVLERNPEYWDKGKPYLDRVVFRVIPDAAARS
AALEKGEVQWGVLSPVPLKDAERLVRLPNLRVSTEGYEWLSPWLFADFNVERGPLRDVKVRQALAQAVDRNAIAKVVWFG
FAKPATSPIPSTLAAFHDAGGIAYDYDPRKAEALLDAAGYKRGPDGTRFTLDIDYIPYGDDFKRTAEYLKQALKRVGVEA
NVRSQDTAAFTRRVYGDRDFDISITWFAAFSDPQVGVNRAYWSGSVGKNIPWTNGSGYRNDKVDALISATQGEADPEKRI
QLFKDLQKTVLTDLPTLPLVELKFFTVEAANLRHPKVRGDQVYGSFRDFWIDDAKVADAPKSN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=462, Percent_Identity=28.7878787878788, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1789966, Length=474, Percent_Identity=28.2700421940928, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1787762, Length=420, Percent_Identity=29.0476190476191, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1787551, Length=495, Percent_Identity=25.2525252525253, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789397, Length=479, Percent_Identity=27.5574112734864, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789887, Length=526, Percent_Identity=23.574144486692, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787495, Length=533, Percent_Identity=23.4521575984991, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI87081878, Length=490, Percent_Identity=23.265306122449, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87082063, Length=498, Percent_Identity=21.8875502008032, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59874; Mature: 59743

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHLARWKAGAVLAVAAVLVAGAAGAQQAGDTPKRGGTLSAIIQPEPVILTAALNTAAPT
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCC
GIVTGSIFDGLVDYDSNLRPVAALAESWETAKDGLTFTLHLRGGVKWHDGKPFTSADVKW
CEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCEE
TLEEVWKKIHPRNQAIFAKVTSVDTPDDRTVVLHLSQPSVAILSSLNSNGAQVLPKHLYE
HHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHC
GTDILNNPYNNKPVGTGPFVFKEWSRGNYIVLERNPEYWDKGKPYLDRVVFRVIPDAAAR
CCHHHCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHH
SAALEKGEVQWGVLSPVPLKDAERLVRLPNLRVSTEGYEWLSPWLFADFNVERGPLRDVK
HHHHCCCCEEEECCCCCCCHHHHHHHHCCCCEEECCCCHHHCCEEEEECCCCCCCCHHHH
VRQALAQAVDRNAIAKVVWFGFAKPATSPIPSTLAAFHDAGGIAYDYDPRKAEALLDAAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEECCCCHHHHHHHHHHC
YKRGPDGTRFTLDIDYIPYGDDFKRTAEYLKQALKRVGVEANVRSQDTAAFTRRVYGDRD
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
FDISITWFAAFSDPQVGVNRAYWSGSVGKNIPWTNGSGYRNDKVDALISATQGEADPEKR
CEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHH
IQLFKDLQKTVLTDLPTLPLVELKFFTVEAANLRHPKVRGDQVYGSFRDFWIDDAKVADA
HHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHCCHHHHCCCCCCCCCC
PKSN
CCCC
>Mature Secondary Structure 
GHLARWKAGAVLAVAAVLVAGAAGAQQAGDTPKRGGTLSAIIQPEPVILTAALNTAAPT
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCC
GIVTGSIFDGLVDYDSNLRPVAALAESWETAKDGLTFTLHLRGGVKWHDGKPFTSADVKW
CEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCEE
TLEEVWKKIHPRNQAIFAKVTSVDTPDDRTVVLHLSQPSVAILSSLNSNGAQVLPKHLYE
HHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHC
GTDILNNPYNNKPVGTGPFVFKEWSRGNYIVLERNPEYWDKGKPYLDRVVFRVIPDAAAR
CCHHHCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHH
SAALEKGEVQWGVLSPVPLKDAERLVRLPNLRVSTEGYEWLSPWLFADFNVERGPLRDVK
HHHHCCCCEEEECCCCCCCHHHHHHHHCCCCEEECCCCHHHCCEEEEECCCCCCCCHHHH
VRQALAQAVDRNAIAKVVWFGFAKPATSPIPSTLAAFHDAGGIAYDYDPRKAEALLDAAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEECCCCHHHHHHHHHHC
YKRGPDGTRFTLDIDYIPYGDDFKRTAEYLKQALKRVGVEANVRSQDTAAFTRRVYGDRD
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
FDISITWFAAFSDPQVGVNRAYWSGSVGKNIPWTNGSGYRNDKVDALISATQGEADPEKR
CEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHH
IQLFKDLQKTVLTDLPTLPLVELKFFTVEAANLRHPKVRGDQVYGSFRDFWIDDAKVADA
HHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHCCHHHHCCCCCCCCCC
PKSN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA