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The map label for this gene is thcD [H]

Identifier: 154246557

GI number: 154246557

Start: 2901328

End: 2902506

Strand: Direct

Name: thcD [H]

Synonym: Xaut_2616

Alternate gene names: 154246557

Gene position: 2901328-2902506 (Clockwise)

Preceding gene: 154246556

Following gene: 154246558

Centisome position: 54.65

GC content: 68.36

Gene sequence:

>1179_bases
ATGAGCGCCCCCGTCGTCATCGTCGGGGCGGGGCAGGCGGCGGCGCAGGCCGTCACCTCCCTGAAGGCGGAAGGGTATGC
CGGCGATATCATCGTCATCGGCGACGAGCCCTATCTGCCCTACCAGCGTCCGCCACTCTCCAAGGCCTATCTCGGCGACG
AGATGACCGAGGACCGGCTCGAATTGAAGGCGCCCAAATTCTATGCGGATGCCGGGGCGGAGCTGCGCCTTGCCACCCGC
GTCGCCCGCCTGCTGCCGTCCGAGAAGGCGGTGGAACTGGCGGACGGCTCCCGTCTCGCCTACGGCGCGCTGCTGATCGC
CACCGGCACTCGCGCCCGCGCCTTGCCGGTGCCGGGGGCGGAACTGGCGGGCCTCTTCTCCATCCGCTCCATCGATGACG
TGAAGCACTTCCGTGCCTCAGCCGTGCCGGGAGCGAAGCTGGTGATCATCGGTGGCGGCTATATCGGCCTTGAGGTGGCG
GCCAAGGCGAAGAAGCTCGGCCTCGAAGTCACCGTGGTGGAAGGCCAGCCGCGCCTGCTCGCCCGCGTCGCCTGCGCCAC
CATCTCCGATTTCGCCCGCACCCTGCATGAGGGCAATGGCGTCACCATCCTCACCGGCATGGGCGTGGCCCGGCTGGTGG
GCACGGAGCGGGTGACGGGGGTGGAACTTGCGGATGGGCGGGTGCTGCCGGCCGATCTTGTTCTGTCCGCCGTGGGCGCG
GTGCCCAATGCGGAGCTGGCCTCCGAGGCGGGGCTGGTGCTGGAGAACGGCATAAGCGTGGACGAGGCCACCCGCACCAG
CGCGCCCGACATCTATGCCGCCGGCGATGTCGCCTCTTTTCCGAGCAGGCTCTACGGCCGGCGGGTTCGCCTTGAATCCG
TGCAGAATGCCATCGATCAGGCCAAGGCTGCCGCAAAAGCGATCACGGGTGGTGGCGTTGTATACGATCCCATCCCCTGG
TTCTGGTCCGATCAATACGATGTGAAGCTCCAGATCGCGGGGCTCCTCGACGGCTGCGAGCGCACCGAAACCGAGGGCGA
AGTGGCCGCCGGACGCTTCTGCGTGCGGTATTTCGCGGGGGATCGGCTGCTGGCGGTCTGCTCGGTCAACGATCCAAAGA
GCCACATGCTCGCCCGCAAGGCCCTCGCCACGCCGCCACCGGTGCCTGCCCCCGGCTAG

Upstream 100 bases:

>100_bases
GCTCGCCCTCCTATGTGCTGAATGGCGAGCTCTATTTCGGCCAGGACCGGCTGGACTTTGTCGCCGCCGCGCTCGCGGCC
GGAGAGCCGGGGGCGGCGCT

Downstream 100 bases:

>100_bases
GGGTGGCACGGGCATTGCCATCACCAAGCGTCACCTGCCCGCCGAATCCTCGCACTCGTCTTCCGGAGCGCCCTTATGTC
GATGTTCTCGAACCCGTTCT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSAPVVIVGAGQAAAQAVTSLKAEGYAGDIIVIGDEPYLPYQRPPLSKAYLGDEMTEDRLELKAPKFYADAGAELRLATR
VARLLPSEKAVELADGSRLAYGALLIATGTRARALPVPGAELAGLFSIRSIDDVKHFRASAVPGAKLVIIGGGYIGLEVA
AKAKKLGLEVTVVEGQPRLLARVACATISDFARTLHEGNGVTILTGMGVARLVGTERVTGVELADGRVLPADLVLSAVGA
VPNAELASEAGLVLENGISVDEATRTSAPDIYAAGDVASFPSRLYGRRVRLESVQNAIDQAKAAAKAITGGGVVYDPIPW
FWSDQYDVKLQIAGLLDGCERTETEGEVAAGRFCVRYFAGDRLLAVCSVNDPKSHMLARKALATPPPVPAPG

Sequences:

>Translated_392_residues
MSAPVVIVGAGQAAAQAVTSLKAEGYAGDIIVIGDEPYLPYQRPPLSKAYLGDEMTEDRLELKAPKFYADAGAELRLATR
VARLLPSEKAVELADGSRLAYGALLIATGTRARALPVPGAELAGLFSIRSIDDVKHFRASAVPGAKLVIIGGGYIGLEVA
AKAKKLGLEVTVVEGQPRLLARVACATISDFARTLHEGNGVTILTGMGVARLVGTERVTGVELADGRVLPADLVLSAVGA
VPNAELASEAGLVLENGISVDEATRTSAPDIYAAGDVASFPSRLYGRRVRLESVQNAIDQAKAAAKAITGGGVVYDPIPW
FWSDQYDVKLQIAGLLDGCERTETEGEVAAGRFCVRYFAGDRLLAVCSVNDPKSHMLARKALATPPPVPAPG
>Mature_391_residues
SAPVVIVGAGQAAAQAVTSLKAEGYAGDIIVIGDEPYLPYQRPPLSKAYLGDEMTEDRLELKAPKFYADAGAELRLATRV
ARLLPSEKAVELADGSRLAYGALLIATGTRARALPVPGAELAGLFSIRSIDDVKHFRASAVPGAKLVIIGGGYIGLEVAA
KAKKLGLEVTVVEGQPRLLARVACATISDFARTLHEGNGVTILTGMGVARLVGTERVTGVELADGRVLPADLVLSAVGAV
PNAELASEAGLVLENGISVDEATRTSAPDIYAAGDVASFPSRLYGRRVRLESVQNAIDQAKAAAKAITGGGVVYDPIPWF
WSDQYDVKLQIAGLLDGCERTETEGEVAAGRFCVRYFAGDRLLAVCSVNDPKSHMLARKALATPPPVPAPG

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=376, Percent_Identity=28.9893617021277, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI65787454, Length=376, Percent_Identity=28.9893617021277, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI21389617, Length=376, Percent_Identity=28.9893617021277, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI4757732, Length=369, Percent_Identity=28.9972899728997, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI22202629, Length=369, Percent_Identity=28.9972899728997, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI195927006, Length=185, Percent_Identity=29.1891891891892, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788892, Length=335, Percent_Identity=37.910447761194, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789765, Length=288, Percent_Identity=29.5138888888889, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789065, Length=289, Percent_Identity=29.0657439446367, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=372, Percent_Identity=30.3763440860215, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI32564386, Length=339, Percent_Identity=27.7286135693215, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24639257, Length=325, Percent_Identity=29.5384615384615, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281359715, Length=336, Percent_Identity=29.1666666666667, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281359713, Length=336, Percent_Identity=29.1666666666667, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24639250, Length=336, Percent_Identity=29.1666666666667, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI18543267, Length=336, Percent_Identity=29.1666666666667, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24639252, Length=336, Percent_Identity=29.1666666666667, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24585130, Length=328, Percent_Identity=26.219512195122, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28573993, Length=357, Percent_Identity=26.890756302521, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=26.890756302521, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 40923; Mature: 40792

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVVIVGAGQAAAQAVTSLKAEGYAGDIIVIGDEPYLPYQRPPLSKAYLGDEMTEDRL
CCCCEEEEECCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCHH
ELKAPKFYADAGAELRLATRVARLLPSEKAVELADGSRLAYGALLIATGTRARALPVPGA
HHCCCEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEEEEEECCCCEECCCCCH
ELAGLFSIRSIDDVKHFRASAVPGAKLVIIGGGYIGLEVAAKAKKLGLEVTVVEGQPRLL
HHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCEEEEHHHHHHHHCCEEEEEECCCCHHH
ARVACATISDFARTLHEGNGVTILTGMGVARLVGTERVTGVELADGRVLPADLVLSAVGA
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCEEECCCEECHHHHHHHHHCC
VPNAELASEAGLVLENGISVDEATRTSAPDIYAAGDVASFPSRLYGRRVRLESVQNAIDQ
CCCCHHHHHCCEEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCHHHHHHHHHHHHH
AKAAAKAITGGGVVYDPIPWFWSDQYDVKLQIAGLLDGCERTETEGEVAAGRFCVRYFAG
HHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEECCCHHCCCCCCCHHHHHHHEEEECC
DRLLAVCSVNDPKSHMLARKALATPPPVPAPG
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SAPVVIVGAGQAAAQAVTSLKAEGYAGDIIVIGDEPYLPYQRPPLSKAYLGDEMTEDRL
CCCEEEEECCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCHH
ELKAPKFYADAGAELRLATRVARLLPSEKAVELADGSRLAYGALLIATGTRARALPVPGA
HHCCCEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEEEEEECCCCEECCCCCH
ELAGLFSIRSIDDVKHFRASAVPGAKLVIIGGGYIGLEVAAKAKKLGLEVTVVEGQPRLL
HHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCEEEEHHHHHHHHCCEEEEEECCCCHHH
ARVACATISDFARTLHEGNGVTILTGMGVARLVGTERVTGVELADGRVLPADLVLSAVGA
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCEEECCCEECHHHHHHHHHCC
VPNAELASEAGLVLENGISVDEATRTSAPDIYAAGDVASFPSRLYGRRVRLESVQNAIDQ
CCCCHHHHHCCEEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCHHHHHHHHHHHHH
AKAAAKAITGGGVVYDPIPWFWSDQYDVKLQIAGLLDGCERTETEGEVAAGRFCVRYFAG
HHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEECCCHHCCCCCCCHHHHHHHEEEECC
DRLLAVCSVNDPKSHMLARKALATPPPVPAPG
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]