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The map label for this gene is leuB [H]

Identifier: 154246530

GI number: 154246530

Start: 2879018

End: 2880130

Strand: Direct

Name: leuB [H]

Synonym: Xaut_2589

Alternate gene names: 154246530

Gene position: 2879018-2880130 (Clockwise)

Preceding gene: 154246529

Following gene: 154246532

Centisome position: 54.23

GC content: 68.37

Gene sequence:

>1113_bases
ATGGCCACCCACAAGCTGCTCCTCCTCGCCGGCGACGGCATCGGTCCCGAAGTCATGGCCGAGGTGAAACGGGTGGCCGC
ATGGCTCGACCAGGCGGGCCTTGCCTCCTTCGCCATCGAAGAGGATCTGGTGGGCGGCTGCGCCTATGACGCGCACGGCG
CCGCCATCTCGGAGGATGCCATGGTCCGCGCCAAGGCGGCGGACGCGGTGCTGCTCGGCGCCGTGGGCGGCCCCAAGTGG
GCGAGCGTACCCTACGAGGCGCGCCCCGAGGCGGGCCTGCTGCGCCTGCGCAAGGACCTGCAGCTGTTCGCCAATCTGCG
CCCCGCCATCTGCTATCCGGCGCTGGCCGATGCCTCCTCCCTCAAGCGCGAGGTGGTGGAGGGGCTCGACATCCTCATCG
TGCGCGAGCTGACCGGCGGCGTCTATTTCGGCGAGCCCAAGACCATCATGGACCTCGGCAACGGCCAGAAGCGCGCCATC
GACACGCAGGTCTACGACACCTACGAGATCGAGCGCATCGCTGCCGTGGCCTTCGAGCTGGCCCGCACCCGCCGCAACAA
GGTGACCTCTTCCGAAAAGCACAACGTGATGAAGTCCGGCGTGCTCTGGAAGGAGGTGGTCACCGCCCTCCACGAGCGCG
CCTACAAGGACGTGGAGCTGGAGCACAACCTCGCCGATTCGCTCGCCATGCAGCTCGTGCGCTGGCCCAAGCAGTATGAC
GTGATCGTCACCGACAATCTGTTCGGCGACATCCTTTCCGACATCGCTGCCATGCTCACCGGCTCGCTGGGCATGCTGCC
CTCCGCCTCGCTGGGCGCGGCCGATCCGGTCACCGGCAAGCGCGCGGCGCTGTACGAGCCGGTGCACGGCTCGGCCCCCG
ACATTGCCGGCAAGGGCATCGCCAATCCCATCGCCATGATCGGCTCGCTGGCCATGGCCCTGCGCTATTCGTTCAACCAG
GGCGAGGTGGCGGACCGCATCGACCAGGCCATCGCCGGCGTGCTGGCCTCCGGCAAGCGCACTGGCGATATCGCCGGCCC
GGGCACCGAGACCATCGGCACCCAGGACATGGGCAAGGCCATCATCGCCGAGCTGGATGCTGCCACCGCCTGA

Upstream 100 bases:

>100_bases
TGGCGACAGCGCCAAGACGGCTCGATGCACGACTGCTCGATGCACCACTGCTCGACGCCGCAGGCCAAGAGAACTCAAAG
AGACTCCAAGGACACCACCC

Downstream 100 bases:

>100_bases
TCAGGTGCGAACAAGCCCGCGCAGGGCTTGAGCCATATCTTCTCGGCACCGGTCAAGGACCGGGGCCGTTGGCTTTACTC
AACGAAAACGCGCCGTCCGG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MATHKLLLLAGDGIGPEVMAEVKRVAAWLDQAGLASFAIEEDLVGGCAYDAHGAAISEDAMVRAKAADAVLLGAVGGPKW
ASVPYEARPEAGLLRLRKDLQLFANLRPAICYPALADASSLKREVVEGLDILIVRELTGGVYFGEPKTIMDLGNGQKRAI
DTQVYDTYEIERIAAVAFELARTRRNKVTSSEKHNVMKSGVLWKEVVTALHERAYKDVELEHNLADSLAMQLVRWPKQYD
VIVTDNLFGDILSDIAAMLTGSLGMLPSASLGAADPVTGKRAALYEPVHGSAPDIAGKGIANPIAMIGSLAMALRYSFNQ
GEVADRIDQAIAGVLASGKRTGDIAGPGTETIGTQDMGKAIIAELDAATA

Sequences:

>Translated_370_residues
MATHKLLLLAGDGIGPEVMAEVKRVAAWLDQAGLASFAIEEDLVGGCAYDAHGAAISEDAMVRAKAADAVLLGAVGGPKW
ASVPYEARPEAGLLRLRKDLQLFANLRPAICYPALADASSLKREVVEGLDILIVRELTGGVYFGEPKTIMDLGNGQKRAI
DTQVYDTYEIERIAAVAFELARTRRNKVTSSEKHNVMKSGVLWKEVVTALHERAYKDVELEHNLADSLAMQLVRWPKQYD
VIVTDNLFGDILSDIAAMLTGSLGMLPSASLGAADPVTGKRAALYEPVHGSAPDIAGKGIANPIAMIGSLAMALRYSFNQ
GEVADRIDQAIAGVLASGKRTGDIAGPGTETIGTQDMGKAIIAELDAATA
>Mature_369_residues
ATHKLLLLAGDGIGPEVMAEVKRVAAWLDQAGLASFAIEEDLVGGCAYDAHGAAISEDAMVRAKAADAVLLGAVGGPKWA
SVPYEARPEAGLLRLRKDLQLFANLRPAICYPALADASSLKREVVEGLDILIVRELTGGVYFGEPKTIMDLGNGQKRAID
TQVYDTYEIERIAAVAFELARTRRNKVTSSEKHNVMKSGVLWKEVVTALHERAYKDVELEHNLADSLAMQLVRWPKQYDV
IVTDNLFGDILSDIAAMLTGSLGMLPSASLGAADPVTGKRAALYEPVHGSAPDIAGKGIANPIAMIGSLAMALRYSFNQG
EVADRIDQAIAGVLASGKRTGDIAGPGTETIGTQDMGKAIIAELDAATA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=360, Percent_Identity=32.7777777777778, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28178816, Length=364, Percent_Identity=28.8461538461538, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178821, Length=365, Percent_Identity=28.7671232876712, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4758582, Length=369, Percent_Identity=28.9972899728997, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178838, Length=323, Percent_Identity=29.1021671826625, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI28178819, Length=200, Percent_Identity=33.5, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87081683, Length=362, Percent_Identity=48.8950276243094, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1788101, Length=360, Percent_Identity=35.5555555555556, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787381, Length=314, Percent_Identity=26.1146496815287, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71986051, Length=362, Percent_Identity=33.7016574585635, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17505779, Length=316, Percent_Identity=31.6455696202532, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=362, Percent_Identity=30.3867403314917, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25144293, Length=369, Percent_Identity=28.7262872628726, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=42.0054200542005, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=35.1428571428571, Blast_Score=165, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=369, Percent_Identity=32.2493224932249, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=277, Percent_Identity=32.129963898917, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24643270, Length=351, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24643268, Length=351, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24661184, Length=354, Percent_Identity=31.0734463276836, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161078635, Length=370, Percent_Identity=28.9189189189189, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078633, Length=370, Percent_Identity=28.9189189189189, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24650122, Length=370, Percent_Identity=28.9189189189189, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281362242, Length=364, Percent_Identity=27.7472527472527, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648872, Length=364, Percent_Identity=27.7472527472527, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI161078637, Length=370, Percent_Identity=28.9189189189189, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI161078639, Length=366, Percent_Identity=28.9617486338798, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI20130355, Length=358, Percent_Identity=25.9776536312849, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39207; Mature: 39076

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHKLLLLAGDGIGPEVMAEVKRVAAWLDQAGLASFAIEEDLVGGCAYDAHGAAISEDA
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHH
MVRAKAADAVLLGAVGGPKWASVPYEARPEAGLLRLRKDLQLFANLRPAICYPALADASS
HHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHH
LKREVVEGLDILIVRELTGGVYFGEPKTIMDLGNGQKRAIDTQVYDTYEIERIAAVAFEL
HHHHHHCCHHEEEEEECCCCEEECCCCCEEECCCCCCCEECHHHHHHHHHHHHHHHHHHH
ARTRRNKVTSSEKHNVMKSGVLWKEVVTALHERAYKDVELEHNLADSLAMQLVRWPKQYD
HHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
VIVTDNLFGDILSDIAAMLTGSLGMLPSASLGAADPVTGKRAALYEPVHGSAPDIAGKGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCC
ANPIAMIGSLAMALRYSFNQGEVADRIDQAIAGVLASGKRTGDIAGPGTETIGTQDMGKA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
IIAELDAATA
HHHHHHHCCC
>Mature Secondary Structure 
ATHKLLLLAGDGIGPEVMAEVKRVAAWLDQAGLASFAIEEDLVGGCAYDAHGAAISEDA
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHH
MVRAKAADAVLLGAVGGPKWASVPYEARPEAGLLRLRKDLQLFANLRPAICYPALADASS
HHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHH
LKREVVEGLDILIVRELTGGVYFGEPKTIMDLGNGQKRAIDTQVYDTYEIERIAAVAFEL
HHHHHHCCHHEEEEEECCCCEEECCCCCEEECCCCCCCEECHHHHHHHHHHHHHHHHHHH
ARTRRNKVTSSEKHNVMKSGVLWKEVVTALHERAYKDVELEHNLADSLAMQLVRWPKQYD
HHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
VIVTDNLFGDILSDIAAMLTGSLGMLPSASLGAADPVTGKRAALYEPVHGSAPDIAGKGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCC
ANPIAMIGSLAMALRYSFNQGEVADRIDQAIAGVLASGKRTGDIAGPGTETIGTQDMGKA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
IIAELDAATA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA