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The map label for this gene is rutF [H]

Identifier: 154246516

GI number: 154246516

Start: 2866557

End: 2867066

Strand: Direct

Name: rutF [H]

Synonym: Xaut_2575

Alternate gene names: 154246516

Gene position: 2866557-2867066 (Clockwise)

Preceding gene: 154246515

Following gene: 154246517

Centisome position: 53.99

GC content: 71.18

Gene sequence:

>510_bases
ATGAGCGGCGCCCCAGCCCCCATCGATCCGGCGCTGTTCCGCGAGGCCATGCGGCGCATGGCGTCGGGCGTGGCGGTAAT
CGTCACCGACGGGCCGGCGGGGCGGGCGGGCCTCACCGTCTCCTCGCTCGCCTCTTTGTGCATGGAGCCGCCCTCGCTCA
TCCTTTGCATCCATGAGAAGAGCGCGGCGCTGGCGACCATCGAGGCCAACGGCGTGTTCGTCGCCAACGTGCTCGCCCAT
CACCAGTCGCGGGTCGCCGACGCCTTTGCAGGCCAGATCCCCGAGCTGCGCGAGGACCGCTTCGCTGCCGCCCGCTGGGA
CGTGGCGGCGAGCGGCGCGCCGGTGCTCGCCGACGCGCTCATGAGCTTCGATTGCCGGCTCGCCGCCCACCATGCCTATG
GCACCCACCGCATCGTGGTGGGTGAGGTGACCGCGGTCCACGGCATCGAGGCCGAAGCCGAGCCGCTGCTCTATGCCAAC
CGCGCCTACCGGCGCCTCTCCGCTGCATAA

Upstream 100 bases:

>100_bases
ATCACCTGGCTGCGGTCTACAAGAGCTTCGATTTCGAGGGTCCGCTGGACTTCGTGCGCAAGACCGCCGGTCTCTCCGAT
AAGGTGAAGGGCTCGCTGAA

Downstream 100 bases:

>100_bases
GGGTTTGATCGTGCCCACCCACACCCGCATCCGCCCGTTCAATACCAAGCAGGCCTATCCCGAGCAGAAGCTCGACAATG
ACGTATGCATGGCCGTGCGC

Product: flavin reductase domain-containing protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MSGAPAPIDPALFREAMRRMASGVAVIVTDGPAGRAGLTVSSLASLCMEPPSLILCIHEKSAALATIEANGVFVANVLAH
HQSRVADAFAGQIPELREDRFAAARWDVAASGAPVLADALMSFDCRLAAHHAYGTHRIVVGEVTAVHGIEAEAEPLLYAN
RAYRRLSAA

Sequences:

>Translated_169_residues
MSGAPAPIDPALFREAMRRMASGVAVIVTDGPAGRAGLTVSSLASLCMEPPSLILCIHEKSAALATIEANGVFVANVLAH
HQSRVADAFAGQIPELREDRFAAARWDVAASGAPVLADALMSFDCRLAAHHAYGTHRIVVGEVTAVHGIEAEAEPLLYAN
RAYRRLSAA
>Mature_168_residues
SGAPAPIDPALFREAMRRMASGVAVIVTDGPAGRAGLTVSSLASLCMEPPSLILCIHEKSAALATIEANGVFVANVLAHH
QSRVADAFAGQIPELREDRFAAARWDVAASGAPVLADALMSFDCRLAAHHAYGTHRIVVGEVTAVHGIEAEAEPLLYANR
AYRRLSAA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=159, Percent_Identity=37.1069182389937, Blast_Score=117, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 17723; Mature: 17592

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAPAPIDPALFREAMRRMASGVAVIVTDGPAGRAGLTVSSLASLCMEPPSLILCIHEK
CCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEECC
SAALATIEANGVFVANVLAHHQSRVADAFAGQIPELREDRFAAARWDVAASGAPVLADAL
CCEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
MSFDCRLAAHHAYGTHRIVVGEVTAVHGIEAEAEPLLYANRAYRRLSAA
HHHCHHEEECCCCCCEEEEEECEEEEECCCCCCCCEEHHHHHHHHHCCC
>Mature Secondary Structure 
SGAPAPIDPALFREAMRRMASGVAVIVTDGPAGRAGLTVSSLASLCMEPPSLILCIHEK
CCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEECC
SAALATIEANGVFVANVLAHHQSRVADAFAGQIPELREDRFAAARWDVAASGAPVLADAL
CCEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
MSFDCRLAAHHAYGTHRIVVGEVTAVHGIEAEAEPLLYANRAYRRLSAA
HHHCHHEEECCCCCCEEEEEECEEEEECCCCCCCCEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA