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The map label for this gene is 154246515

Identifier: 154246515

GI number: 154246515

Start: 2865106

End: 2866560

Strand: Direct

Name: 154246515

Synonym: Xaut_2574

Alternate gene names: NA

Gene position: 2865106-2866560 (Clockwise)

Preceding gene: 154246512

Following gene: 154246516

Centisome position: 53.97

GC content: 65.09

Gene sequence:

>1455_bases
ATGATCAGGACCGGAGCCGCCTACAAGGACGGCCTGCGCGACGGGCGCGAGATCTGGATCGACGGCGAGCGGGTGAAGGA
TGTCACCACCCATGCCGCCTTCAAGCCCATCGTGGACATCAAGGCGCGCATGTACGACCTCCACCACGAGGAGGCCACCG
CCCCGGCCCTGACCTATGTGGAAGACAATGCGGTGCATTCCATCTTCCACCAGCCGCCCACCACGCAGGACCACTGGCAC
GCCAAGCGGCAGGGCGTGGACATCCTTTTGAAGGACGTGGGCGGGGTGGTCACCCGCGTCGGCGACGAGACCATCGGCGA
GATGTGGTCGCTGCAGGACGGCCGCGACATGCTGGCGGCCATCGACCCGCGCTTTGCCGCCAACATCGACAACCACATCA
AGCGCGTGGTGGAGGAAGACCCCTTCCACGTCTCCGCCAACACCGATCCCAAGGGCGACCGCTCCCGCCACATCTCCGAG
CGCGACCCGGACATGATGGTGCATGTCACCAAGGAGACCGACGCCGGCATCATCATCCGCGGCGCCAAGTACGAGACCGC
CGCCGCCTATGCGGACCAGGCCTATCTGAAGCCCACCGTGGGCGCCTGGACCGACGAGAAGGAAAGCGACTACGCGCTCG
GCTGCATCGTGAAGATGGGCGCGCCGGGGGTGAAGCACATCTGCCGTTCGGGCTTCGCCGGCCGTACCAACCCGGCCGAC
TATCCTCTCGCCAATCGCTATGACGAGGTGGATACGTTGGTGATCTTCGACGACGTTCTCATCCCGTGGGAGGACGTGTT
CTTCTATCGCTACACCAAGGCGGCCCAGTTCGTGCGCGGCACGCTGCACCGCTACTCGGCCTATCCTTACGTGCTGCGCC
TGCTCTACGTGGCCGACATGATGATCGGCGCGGCACTGTGGAACGCCAAGCAGACCGGCCTCGACAAGCTGCAGTCGGTG
CAGGAAAAGCTGGCGGACCTTGCGGTGTATCGCGAGGGCATCAACGCCCACCTCACCGCCTCCATCGCGCTGGCCGAGAA
GAGCCCGGCGGGGCTGCTCATGCCCAACCAGTCCATCCTCTATGCCGGCCGTGTGCTCGCCTGCTCGCAGCTTCCGGCGA
TGATGCACGCCACCCGCGAGCTGATCGGCGGCCAGATCTGCGTGACGCCGAACGTGGCGGCGTTCGAGGCCGAGGGCACC
TCGGACTGGCTGAAGAAGTTCTACACCCTCAACGACAATTGGGAGTCGGAGGACCGCCGCAAGCTGCTCGCCTTCGCCCG
CGATCTCGTCTCCTCCGACTATGCCGGCCACCGCCTCGCCTTCGTGCAGTTCGCGCAGGCGCCGCACTTCAATCACCTGG
CTGCGGTCTACAAGAGCTTCGATTTCGAGGGTCCGCTGGACTTCGTGCGCAAGACCGCCGGTCTCTCCGATAAGGTGAAG
GGCTCGCTGAAATGA

Upstream 100 bases:

>100_bases
GCCGATCTTTTCGCCTCAGAGAAAATATTTTATCGATCCCGGCGCCGGCCCCACACTCTGCACCAATCCTAGGCATGACC
AATTCGGGAGCAGGCGCGAG

Downstream 100 bases:

>100_bases
GCGGCGCCCCAGCCCCCATCGATCCGGCGCTGTTCCGCGAGGCCATGCGGCGCATGGCGTCGGGCGTGGCGGTAATCGTC
ACCGACGGGCCGGCGGGGCG

Product: 4-hydroxyphenylacetate 3-hydroxylase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MIRTGAAYKDGLRDGREIWIDGERVKDVTTHAAFKPIVDIKARMYDLHHEEATAPALTYVEDNAVHSIFHQPPTTQDHWH
AKRQGVDILLKDVGGVVTRVGDETIGEMWSLQDGRDMLAAIDPRFAANIDNHIKRVVEEDPFHVSANTDPKGDRSRHISE
RDPDMMVHVTKETDAGIIIRGAKYETAAAYADQAYLKPTVGAWTDEKESDYALGCIVKMGAPGVKHICRSGFAGRTNPAD
YPLANRYDEVDTLVIFDDVLIPWEDVFFYRYTKAAQFVRGTLHRYSAYPYVLRLLYVADMMIGAALWNAKQTGLDKLQSV
QEKLADLAVYREGINAHLTASIALAEKSPAGLLMPNQSILYAGRVLACSQLPAMMHATRELIGGQICVTPNVAAFEAEGT
SDWLKKFYTLNDNWESEDRRKLLAFARDLVSSDYAGHRLAFVQFAQAPHFNHLAAVYKSFDFEGPLDFVRKTAGLSDKVK
GSLK

Sequences:

>Translated_484_residues
MIRTGAAYKDGLRDGREIWIDGERVKDVTTHAAFKPIVDIKARMYDLHHEEATAPALTYVEDNAVHSIFHQPPTTQDHWH
AKRQGVDILLKDVGGVVTRVGDETIGEMWSLQDGRDMLAAIDPRFAANIDNHIKRVVEEDPFHVSANTDPKGDRSRHISE
RDPDMMVHVTKETDAGIIIRGAKYETAAAYADQAYLKPTVGAWTDEKESDYALGCIVKMGAPGVKHICRSGFAGRTNPAD
YPLANRYDEVDTLVIFDDVLIPWEDVFFYRYTKAAQFVRGTLHRYSAYPYVLRLLYVADMMIGAALWNAKQTGLDKLQSV
QEKLADLAVYREGINAHLTASIALAEKSPAGLLMPNQSILYAGRVLACSQLPAMMHATRELIGGQICVTPNVAAFEAEGT
SDWLKKFYTLNDNWESEDRRKLLAFARDLVSSDYAGHRLAFVQFAQAPHFNHLAAVYKSFDFEGPLDFVRKTAGLSDKVK
GSLK
>Mature_484_residues
MIRTGAAYKDGLRDGREIWIDGERVKDVTTHAAFKPIVDIKARMYDLHHEEATAPALTYVEDNAVHSIFHQPPTTQDHWH
AKRQGVDILLKDVGGVVTRVGDETIGEMWSLQDGRDMLAAIDPRFAANIDNHIKRVVEEDPFHVSANTDPKGDRSRHISE
RDPDMMVHVTKETDAGIIIRGAKYETAAAYADQAYLKPTVGAWTDEKESDYALGCIVKMGAPGVKHICRSGFAGRTNPAD
YPLANRYDEVDTLVIFDDVLIPWEDVFFYRYTKAAQFVRGTLHRYSAYPYVLRLLYVADMMIGAALWNAKQTGLDKLQSV
QEKLADLAVYREGINAHLTASIALAEKSPAGLLMPNQSILYAGRVLACSQLPAMMHATRELIGGQICVTPNVAAFEAEGT
SDWLKKFYTLNDNWESEDRRKLLAFARDLVSSDYAGHRLAFVQFAQAPHFNHLAAVYKSFDFEGPLDFVRKTAGLSDKVK
GSLK

Specific function: Utilizes FADH(2) supplied by hpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 54063; Mature: 54063

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTGAAYKDGLRDGREIWIDGERVKDVTTHAAFKPIVDIKARMYDLHHEEATAPALTYV
CCCCCCCHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
EDNAVHSIFHQPPTTQDHWHAKRQGVDILLKDVGGVVTRVGDETIGEMWSLQDGRDMLAA
CCCHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
IDPRFAANIDNHIKRVVEEDPFHVSANTDPKGDRSRHISERDPDMMVHVTKETDAGIIIR
CCCHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEE
GAKYETAAAYADQAYLKPTVGAWTDEKESDYALGCIVKMGAPGVKHICRSGFAGRTNPAD
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCC
YPLANRYDEVDTLVIFDDVLIPWEDVFFYRYTKAAQFVRGTLHRYSAYPYVLRLLYVADM
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MIGAALWNAKQTGLDKLQSVQEKLADLAVYREGINAHLTASIALAEKSPAGLLMPNQSIL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEECCCCCHH
YAGRVLACSQLPAMMHATRELIGGQICVTPNVAAFEAEGTSDWLKKFYTLNDNWESEDRR
HHCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCHHHHHHHEECCCCCCCHHHH
KLLAFARDLVSSDYAGHRLAFVQFAQAPHFNHLAAVYKSFDFEGPLDFVRKTAGLSDKVK
HHHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCC
GSLK
CCCC
>Mature Secondary Structure
MIRTGAAYKDGLRDGREIWIDGERVKDVTTHAAFKPIVDIKARMYDLHHEEATAPALTYV
CCCCCCCHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
EDNAVHSIFHQPPTTQDHWHAKRQGVDILLKDVGGVVTRVGDETIGEMWSLQDGRDMLAA
CCCHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
IDPRFAANIDNHIKRVVEEDPFHVSANTDPKGDRSRHISERDPDMMVHVTKETDAGIIIR
CCCHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEE
GAKYETAAAYADQAYLKPTVGAWTDEKESDYALGCIVKMGAPGVKHICRSGFAGRTNPAD
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCC
YPLANRYDEVDTLVIFDDVLIPWEDVFFYRYTKAAQFVRGTLHRYSAYPYVLRLLYVADM
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MIGAALWNAKQTGLDKLQSVQEKLADLAVYREGINAHLTASIALAEKSPAGLLMPNQSIL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEECCCCCHH
YAGRVLACSQLPAMMHATRELIGGQICVTPNVAAFEAEGTSDWLKKFYTLNDNWESEDRR
HHCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCHHHHHHHEECCCCCCCHHHH
KLLAFARDLVSSDYAGHRLAFVQFAQAPHFNHLAAVYKSFDFEGPLDFVRKTAGLSDKVK
HHHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCC
GSLK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA