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The map label for this gene is acsA2 [H]

Identifier: 154245803

GI number: 154245803

Start: 2092984

End: 2094495

Strand: Reverse

Name: acsA2 [H]

Synonym: Xaut_1859

Alternate gene names: 154245803

Gene position: 2094495-2092984 (Counterclockwise)

Preceding gene: 154245811

Following gene: 154245802

Centisome position: 39.45

GC content: 63.89

Gene sequence:

>1512_bases
ATGGCGCACACCGGCCTGTCGAAGCCCACGACCCACCGCCTGGCGGCGACGCTGGTTCACGAGGGCCTGCTCGCCTATGA
CCCGGCGTCCCGCCTCTACACCCTCGGCGCCTTCTGCCTGAGCCTCGGCCGCCGTGCGAGCGCGATGCCGGCGGAGGCCG
CGACGGAAAGCGATGCCGCATCGGTTCCGAGCCTGTACCAACGGCCGGAATATCGTGGCGACGCCATTCCCCTCGCTCAT
CTGCTGTGCGACCGGCACCTGCCCGGCAAGCGCTCGCATCCGGCCATGATCCATGAATCCGTCTCGGGCCAGACATCGGA
ACTGAGCTTTGGCAGGCTTGCCGACTATTCATCTCGCTTTGCTCGTGTATTGGCGACCATCGGCATCAGGAAAGGCGATT
GCGTTGCCGTCATGCTGCCGAAGGGCGTCGAACTGATCATCGCCGCGCTCGCCATCTGGCGGGTTGGCGCCGTCTACATG
CCGCTGTTCTCCACCTATTCCGCCTCGGCGGTGAACGCCCGCCTGGAGTCGAGCGACGTAAAAGCCATCGTTGCAAATCG
GGTGCTGGTGGAGCGCGCCCGCAAATACATCAAGCCATCAACGCCGGTCTTTCTCGTGGAAGGAGACCACATCAACCGCA
TAGAAGGCCGGGTCACCCAGTTCTGGTCATCGATCTACGAGGCCGAGCCGCTGGCCGAGGCCGCGACCTATGCGGCAGAC
GACCCTTTCCTCATGACATATTCAACGAAAACGGTCGAACCCAGGTACGGACTGTTCACCCCTGTCCGCGCCCTGGCCGC
AATCGAGCAATACATGCGCCTCGGGCTTGATGTCCGGGACGAGGACGTGTTCTGGAACATGACGGATCAGGGCTGGGAAT
ACGGGATCTTCTACGGTCTCGTCGGCCCGCTCCTGATCGGCACGTCCATCCTGTTCTGCGATGGACCCTACGATGTCTCG
CAGGGCTATCGCGTCCTGTCGAAATTCCGTGTCACCAACCTCACCGCCGCCCCCTCCCAGATCAAGGCTTGGCGGCGCGG
CGATCCCAGCGCGGCCTCGCACCAGCTCGCGCTGCGCGTGGTGACCGTCGGCGGCGAGCCGCTGCCTCGGGACATCATCG
CCTGGGTGACGCAGAAGCTCGGCGTGCCCCTGATCAACCAGTACGGACACCGCGAAGCGGGGATGATCATAGGCATGAAG
CATGACCCGCACGATCCACGCTCCCTGAACCGGGTTTCCGTCGGCCGGGTCGCGCCCGGCTTTTCCCTCGTCGTCCTCGA
CGACAAGGGTGCAGAACTGGGCGTGGGCGCCGAAGGAATACTGGCGATCCACGTCCGGCGCTCGCCGCTTTTCTGGTTTC
GCGCCTACAGCAAGGACGAGGTCGCGACGAAAGCCAGATTCAGGTTCGGAAGCGCCTATTACATCATCGGGGATACCGGG
CACATGGATGCCGACGGCTGCGTCCACTACAGCGGACAGGCATCCCACGCCATCTCGATGCACACGGATTGA

Upstream 100 bases:

>100_bases
CTCTACCTCCCCCAAGGGGGCTCAAAGCTTGTCCCGCGCCGTTGCCGTTCTGCGGGCGGTGGTCGACGCGCCGACCCATG
GCGCGCGGCTTGCCGACCTC

Downstream 100 bases:

>100_bases
TATCGCGCCCTGTCGGTCGCCATGTCGTCGTCTCCCTGCGTTCTTTGCGCCCCGCTCATGCGGCTGGCCTGGCGCGTGCC
CGGTCGTTTCGCCGGGTTCA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acetate--CoA ligase 2; Acyl-activating enzyme 2 [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MAHTGLSKPTTHRLAATLVHEGLLAYDPASRLYTLGAFCLSLGRRASAMPAEAATESDAASVPSLYQRPEYRGDAIPLAH
LLCDRHLPGKRSHPAMIHESVSGQTSELSFGRLADYSSRFARVLATIGIRKGDCVAVMLPKGVELIIAALAIWRVGAVYM
PLFSTYSASAVNARLESSDVKAIVANRVLVERARKYIKPSTPVFLVEGDHINRIEGRVTQFWSSIYEAEPLAEAATYAAD
DPFLMTYSTKTVEPRYGLFTPVRALAAIEQYMRLGLDVRDEDVFWNMTDQGWEYGIFYGLVGPLLIGTSILFCDGPYDVS
QGYRVLSKFRVTNLTAAPSQIKAWRRGDPSAASHQLALRVVTVGGEPLPRDIIAWVTQKLGVPLINQYGHREAGMIIGMK
HDPHDPRSLNRVSVGRVAPGFSLVVLDDKGAELGVGAEGILAIHVRRSPLFWFRAYSKDEVATKARFRFGSAYYIIGDTG
HMDADGCVHYSGQASHAISMHTD

Sequences:

>Translated_503_residues
MAHTGLSKPTTHRLAATLVHEGLLAYDPASRLYTLGAFCLSLGRRASAMPAEAATESDAASVPSLYQRPEYRGDAIPLAH
LLCDRHLPGKRSHPAMIHESVSGQTSELSFGRLADYSSRFARVLATIGIRKGDCVAVMLPKGVELIIAALAIWRVGAVYM
PLFSTYSASAVNARLESSDVKAIVANRVLVERARKYIKPSTPVFLVEGDHINRIEGRVTQFWSSIYEAEPLAEAATYAAD
DPFLMTYSTKTVEPRYGLFTPVRALAAIEQYMRLGLDVRDEDVFWNMTDQGWEYGIFYGLVGPLLIGTSILFCDGPYDVS
QGYRVLSKFRVTNLTAAPSQIKAWRRGDPSAASHQLALRVVTVGGEPLPRDIIAWVTQKLGVPLINQYGHREAGMIIGMK
HDPHDPRSLNRVSVGRVAPGFSLVVLDDKGAELGVGAEGILAIHVRRSPLFWFRAYSKDEVATKARFRFGSAYYIIGDTG
HMDADGCVHYSGQASHAISMHTD
>Mature_502_residues
AHTGLSKPTTHRLAATLVHEGLLAYDPASRLYTLGAFCLSLGRRASAMPAEAATESDAASVPSLYQRPEYRGDAIPLAHL
LCDRHLPGKRSHPAMIHESVSGQTSELSFGRLADYSSRFARVLATIGIRKGDCVAVMLPKGVELIIAALAIWRVGAVYMP
LFSTYSASAVNARLESSDVKAIVANRVLVERARKYIKPSTPVFLVEGDHINRIEGRVTQFWSSIYEAEPLAEAATYAADD
PFLMTYSTKTVEPRYGLFTPVRALAAIEQYMRLGLDVRDEDVFWNMTDQGWEYGIFYGLVGPLLIGTSILFCDGPYDVSQ
GYRVLSKFRVTNLTAAPSQIKAWRRGDPSAASHQLALRVVTVGGEPLPRDIIAWVTQKLGVPLINQYGHREAGMIIGMKH
DPHDPRSLNRVSVGRVAPGFSLVVLDDKGAELGVGAEGILAIHVRRSPLFWFRAYSKDEVATKARFRFGSAYYIIGDTGH
MDADGCVHYSGQASHAISMHTD

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=476, Percent_Identity=25, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI13375727, Length=452, Percent_Identity=24.7787610619469, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI115511026, Length=446, Percent_Identity=26.457399103139, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI122937307, Length=422, Percent_Identity=23.9336492890995, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI38505220, Length=481, Percent_Identity=25.1559251559252, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI58082049, Length=435, Percent_Identity=25.2873563218391, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI8923896, Length=450, Percent_Identity=25.7777777777778, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI195947378, Length=409, Percent_Identity=23.2273838630807, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI253970489, Length=463, Percent_Identity=24.622030237581, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI28416953, Length=442, Percent_Identity=22.8506787330317, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1790505, Length=461, Percent_Identity=26.0303687635575, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786529, Length=451, Percent_Identity=25.4988913525499, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693145, Length=411, Percent_Identity=22.6277372262774, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI221142682, Length=425, Percent_Identity=24.2352941176471, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI71983001, Length=443, Percent_Identity=24.3792325056433, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=443, Percent_Identity=24.3792325056433, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319264, Length=487, Percent_Identity=25.4620123203285, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323182, Length=324, Percent_Identity=29.320987654321, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355225, Length=454, Percent_Identity=24.4493392070485, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI62472339, Length=436, Percent_Identity=25.4587155963303, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24667955, Length=436, Percent_Identity=25.4587155963303, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24667959, Length=405, Percent_Identity=23.9506172839506, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011904
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 55139; Mature: 55008

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHTGLSKPTTHRLAATLVHEGLLAYDPASRLYTLGAFCLSLGRRASAMPAEAATESDAA
CCCCCCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHH
SVPSLYQRPEYRGDAIPLAHLLCDRHLPGKRSHPAMIHESVSGQTSELSFGRLADYSSRF
CCCHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
ARVLATIGIRKGDCVAVMLPKGVELIIAALAIWRVGAVYMPLFSTYSASAVNARLESSDV
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCH
KAIVANRVLVERARKYIKPSTPVFLVEGDHINRIEGRVTQFWSSIYEAEPLAEAATYAAD
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCC
DPFLMTYSTKTVEPRYGLFTPVRALAAIEQYMRLGLDVRDEDVFWNMTDQGWEYGIFYGL
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHH
VGPLLIGTSILFCDGPYDVSQGYRVLSKFRVTNLTAAPSQIKAWRRGDPSAASHQLALRV
HHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCEECCHHHHHHHHCCCCCCCCCEEEEEE
VTVGGEPLPRDIIAWVTQKLGVPLINQYGHREAGMIIGMKHDPHDPRSLNRVSVGRVAPG
EEECCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCC
FSLVVLDDKGAELGVGAEGILAIHVRRSPLFWFRAYSKDEVATKARFRFGSAYYIIGDTG
EEEEEECCCCCEECCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHEEECCEEEEEECCC
HMDADGCVHYSGQASHAISMHTD
CCCCCCEEEECCCCCCEEEECCC
>Mature Secondary Structure 
AHTGLSKPTTHRLAATLVHEGLLAYDPASRLYTLGAFCLSLGRRASAMPAEAATESDAA
CCCCCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHH
SVPSLYQRPEYRGDAIPLAHLLCDRHLPGKRSHPAMIHESVSGQTSELSFGRLADYSSRF
CCCHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
ARVLATIGIRKGDCVAVMLPKGVELIIAALAIWRVGAVYMPLFSTYSASAVNARLESSDV
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCH
KAIVANRVLVERARKYIKPSTPVFLVEGDHINRIEGRVTQFWSSIYEAEPLAEAATYAAD
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCC
DPFLMTYSTKTVEPRYGLFTPVRALAAIEQYMRLGLDVRDEDVFWNMTDQGWEYGIFYGL
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHH
VGPLLIGTSILFCDGPYDVSQGYRVLSKFRVTNLTAAPSQIKAWRRGDPSAASHQLALRV
HHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCEECCHHHHHHHHCCCCCCCCCEEEEEE
VTVGGEPLPRDIIAWVTQKLGVPLINQYGHREAGMIIGMKHDPHDPRSLNRVSVGRVAPG
EEECCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCC
FSLVVLDDKGAELGVGAEGILAIHVRRSPLFWFRAYSKDEVATKARFRFGSAYYIIGDTG
EEEEEECCCCCEECCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHEEECCEEEEEECCC
HMDADGCVHYSGQASHAISMHTD
CCCCCCEEEECCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]