Click here to switch to the map view.

The map label for this gene is thiE

Identifier: 154245811

GI number: 154245811

Start: 2103694

End: 2104329

Strand: Reverse

Name: thiE

Synonym: Xaut_1867

Alternate gene names: 154245811

Gene position: 2104329-2103694 (Counterclockwise)

Preceding gene: 154245812

Following gene: 154245803

Centisome position: 39.64

GC content: 72.96

Gene sequence:

>636_bases
ATGCCCAAGCCCTTCGACCTCACCCTCTATCTCGTCACCGACCCGCGCCTGGTTGCGGCACGCGGCCTGCTGACGACGGT
GGACGCGGCGGTGAAGGGCGGCGCCACCATCGTCCAGCTGCGCGATCCTGACGCCCATGGCCGGGCTCTGGTGGAACAGG
CCCGCGCGCTGAAGGCGCTGCTCGCCCCGCTCGGCATCCCGCTCATCATCAATGACCGGGTGGACGTGGCGGTGGCGGCG
GATGCGGACGGGGTGCATCTGGGCCAGGACGACATGACCCCCGCCGACGCCCGCGCAGTGCTGGGGCCGCAGCGCATCCT
CGGCCTGTCGGTGGGCAATCCGGCCGAATATGCCGCGTCGGATCTTTCCGGCGTGGATTATCTCGGCGTCGGTCCGGTGA
AGGCCACCGGCACCAAGGCCGATGCCGGCGGCGCCATCGGCGCGGCGGGTGTCGGCGCCGTGCGGGCGCTGACGCGCCTT
CCCCTCGTGGGCATCGGCGGCCTTGCTACGGCAGACGTGGCAGACGTGATCCGCGCCGGGGCGGATGGGGTGGCGGTGGT
CTCCTCCATCTGCGCCGCGAGCGACCCCGAGCAGGCCGCCCGCGCGTTGAAGGCGGCGGTCCTGGCCGCGCGCTGA

Upstream 100 bases:

>100_bases
CGAAGGCGGCGTGAGACTGCCCCGTAAGACTGCAGCGTGAGGGGGTCACCATCGATCCCCCACCGCTGGCATGATTTTCC
CCTCCCCCGCAGGACCAAAG

Downstream 100 bases:

>100_bases
GCGCCGGTCTGGAAATTCAAAACGGCGCAACTCCCGGCACCCCGTCATGGCCGGGCTTGTCCCGGGTATCCACGCCGAAC
CGCTTGAAGCAGGCAATTAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MPKPFDLTLYLVTDPRLVAARGLLTTVDAAVKGGATIVQLRDPDAHGRALVEQARALKALLAPLGIPLIINDRVDVAVAA
DADGVHLGQDDMTPADARAVLGPQRILGLSVGNPAEYAASDLSGVDYLGVGPVKATGTKADAGGAIGAAGVGAVRALTRL
PLVGIGGLATADVADVIRAGADGVAVVSSICAASDPEQAARALKAAVLAAR

Sequences:

>Translated_211_residues
MPKPFDLTLYLVTDPRLVAARGLLTTVDAAVKGGATIVQLRDPDAHGRALVEQARALKALLAPLGIPLIINDRVDVAVAA
DADGVHLGQDDMTPADARAVLGPQRILGLSVGNPAEYAASDLSGVDYLGVGPVKATGTKADAGGAIGAAGVGAVRALTRL
PLVGIGGLATADVADVIRAGADGVAVVSSICAASDPEQAARALKAAVLAAR
>Mature_210_residues
PKPFDLTLYLVTDPRLVAARGLLTTVDAAVKGGATIVQLRDPDAHGRALVEQARALKALLAPLGIPLIINDRVDVAVAAD
ADGVHLGQDDMTPADARAVLGPQRILGLSVGNPAEYAASDLSGVDYLGVGPVKATGTKADAGGAIGAAGVGAVRALTRLP
LVGIGGLATADVADVIRAGADGVAVVSSICAASDPEQAARALKAAVLAAR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=195, Percent_Identity=30.2564102564103, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325042, Length=225, Percent_Identity=38.2222222222222, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_XANP2 (A7IGG9)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001416769.1
- STRING:   A7IGG9
- GeneID:   5423806
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_1867
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   PRK00043
- BioCyc:   XAUT78245:XAUT_1867-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 20959; Mature: 20828

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKPFDLTLYLVTDPRLVAARGLLTTVDAAVKGGATIVQLRDPDAHGRALVEQARALKAL
CCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
LAPLGIPLIINDRVDVAVAADADGVHLGQDDMTPADARAVLGPQRILGLSVGNPAEYAAS
HHHCCCCEEECCCCCEEEEECCCCCEECCCCCCCCHHHHHCCHHHHEEECCCCCHHHHHH
DLSGVDYLGVGPVKATGTKADAGGAIGAAGVGAVRALTRLPLVGIGGLATADVADVIRAG
HCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
ADGVAVVSSICAASDPEQAARALKAAVLAAR
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKPFDLTLYLVTDPRLVAARGLLTTVDAAVKGGATIVQLRDPDAHGRALVEQARALKAL
CCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
LAPLGIPLIINDRVDVAVAADADGVHLGQDDMTPADARAVLGPQRILGLSVGNPAEYAAS
HHHCCCCEEECCCCCEEEEECCCCCEECCCCCCCCHHHHHCCHHHHEEECCCCCHHHHHH
DLSGVDYLGVGPVKATGTKADAGGAIGAAGVGAVRALTRLPLVGIGGLATADVADVIRAG
HCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
ADGVAVVSSICAASDPEQAARALKAAVLAAR
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA