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The map label for this gene is murD

Identifier: 154245789

GI number: 154245789

Start: 2075594

End: 2077207

Strand: Reverse

Name: murD

Synonym: Xaut_1845

Alternate gene names: 154245789

Gene position: 2077207-2075594 (Counterclockwise)

Preceding gene: 154245791

Following gene: 154245788

Centisome position: 39.13

GC content: 71.07

Gene sequence:

>1614_bases
ATGAGACGACCATTGCCGCACCGGAATTTTCGCAAAGCCATGATCCCCGTCACCGTCTTCAAGGACCGGACCGTCGCCCT
TTTCGGGCTCGGCGGCTCCGGGCTCGCCACCGCCGAAGCGCTCAAGGCCGGCGGCGCCCATGTGATCGCCTATGACGATG
CCGAGGCCTCCCGCCGCAAGGCCGGCGAGGCCGGCATCCTGGTCCAGGACTTAAGGTCCATCGACTGGACCATCGTGGCC
GCGCTCGTGCTCTCCCCCGGAGTGCCGCTCACCCATCCCGAGCCGCACTGGTCGGTGAAGCTGGCGCGCGACGCCGGCGC
GGAGGTGATCGGCGACATCGAGCTGTTCTGCCGGGAACGGCACGCCATCGCCCGGCGCTCGCCGTTCATCGCCATCACCG
GCACCAACGGCAAGTCCACCACCACGGCGCTCATCACCCACATCCTGCGGGTGGCCGGACGCGACGTGCAGATGGGCGGC
AATATCGGCACCGCCATCCTCTCCCTCGCCCCGCCCAAGCCGGGCCGGGTTCATGTGGTGGAGTGCTCGTCCTACCAGGT
GGACCTCGCCCCGAGCCTCGACCCCTCCGTGGGCGTGATGCTGAACATCACCCCCGACCATCTCGACCGCCACGGCACCA
TCGAGCATTACGCGGCGGTCAAGGAGCGGCTCGTCGCCGGCGTCGAGGGCGGCGGCACCGCCGTGGTGGGCGTGGACGAC
GATTTCTCCGCCGCCATGGCGGACCGGGCCGTCGAGGCGGGCAAGCATGTGGTGCGCATCTCGGTGAAGCGCGCGCTGCC
GGACGGCATCTGGCTCGACGGCGAGACGCTGGTGGTGTCCGAGGGCGGGGCCGAGCGCTTCCGCCTGCCGCTCGCCGGCA
TCGGCTCCCTGCGCGGCACCCACAATGCGCAGAACGCCGCCGCCGCCTTTGCCGCCTGCCGCGGCGTGGGGGTCGCGCCC
GAGATCATCACCATCGCCATGAAGCGCTTCCCCGGCCTTGCCCACCGCATGGAAGAGGTGGGCAACAAGGGCGCGGTGCT
GTTCGTCAACGATTCCAAGGCCACCAACGCGGACGCCGCCGAGCGGGCGCTGGCGAGCTTCACCAGCATCTTCTGGATCG
CCGGCGGCAAGCCCAAGACCGGCGGCATCGCCCCGCTCGCGCCGTTCTTCGATCGCGTGGCGAAGGCCTACCTCATCGGC
GAGGCGGCTGAAGAGTTCGCCACGACCCTGGGCAGCACCGTCGCCCACGAGATCGCCGGCACCCTCGACAAGGCGGTGGC
CGCCGCCGCGCGGGATGCCGAGGCCTCCGGGCTGAAGGATCCGGTGGTGCTGCTGTCGCCAGCCTGCGCCAGCTTCGATC
AGTTCCCCAATTTCGAGGTGCGGGGCGACGCCTTCCGCGAACTGGTGAAACAGGTGCCGGGGGTGGAGGCACGCACCCGC
TCCACCACGCCGGCAGCCCAGAAGGAGCAGCCGAAGGGGGGGCATCCGGGGGGACACCCCGGCGGGCATCCGGGCGGCGC
GCCCCACGGCATGGGTGGCGGACACGGCGGCGGCCGTCCCCACGGCATGGGCAATCCCCATGGCGGCGGCCATCCCGGCA
AGGGGCATCCCTGA

Upstream 100 bases:

>100_bases
ATGTTCCGAATGTTGTCGGCGGTCAACCACGGGATTTCCCGTAAGGCTTCTGTGGTTGCGAAAGGCATTCGCACTTTTGC
CCGTCTTGCTCTAGACGGAA

Downstream 100 bases:

>100_bases
TCGGGGTTTGAGGCGAGGCTTTCCGGCGAAAGGCCACGGCGTCTCGCCGGCCGCGCTTACGCCCGTGCCGTGCCCCCGCC
CTGCCCTCTTCCACACTCGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MRRPLPHRNFRKAMIPVTVFKDRTVALFGLGGSGLATAEALKAGGAHVIAYDDAEASRRKAGEAGILVQDLRSIDWTIVA
ALVLSPGVPLTHPEPHWSVKLARDAGAEVIGDIELFCRERHAIARRSPFIAITGTNGKSTTTALITHILRVAGRDVQMGG
NIGTAILSLAPPKPGRVHVVECSSYQVDLAPSLDPSVGVMLNITPDHLDRHGTIEHYAAVKERLVAGVEGGGTAVVGVDD
DFSAAMADRAVEAGKHVVRISVKRALPDGIWLDGETLVVSEGGAERFRLPLAGIGSLRGTHNAQNAAAAFAACRGVGVAP
EIITIAMKRFPGLAHRMEEVGNKGAVLFVNDSKATNADAAERALASFTSIFWIAGGKPKTGGIAPLAPFFDRVAKAYLIG
EAAEEFATTLGSTVAHEIAGTLDKAVAAAARDAEASGLKDPVVLLSPACASFDQFPNFEVRGDAFRELVKQVPGVEARTR
STTPAAQKEQPKGGHPGGHPGGHPGGAPHGMGGGHGGGRPHGMGNPHGGGHPGKGHP

Sequences:

>Translated_537_residues
MRRPLPHRNFRKAMIPVTVFKDRTVALFGLGGSGLATAEALKAGGAHVIAYDDAEASRRKAGEAGILVQDLRSIDWTIVA
ALVLSPGVPLTHPEPHWSVKLARDAGAEVIGDIELFCRERHAIARRSPFIAITGTNGKSTTTALITHILRVAGRDVQMGG
NIGTAILSLAPPKPGRVHVVECSSYQVDLAPSLDPSVGVMLNITPDHLDRHGTIEHYAAVKERLVAGVEGGGTAVVGVDD
DFSAAMADRAVEAGKHVVRISVKRALPDGIWLDGETLVVSEGGAERFRLPLAGIGSLRGTHNAQNAAAAFAACRGVGVAP
EIITIAMKRFPGLAHRMEEVGNKGAVLFVNDSKATNADAAERALASFTSIFWIAGGKPKTGGIAPLAPFFDRVAKAYLIG
EAAEEFATTLGSTVAHEIAGTLDKAVAAAARDAEASGLKDPVVLLSPACASFDQFPNFEVRGDAFRELVKQVPGVEARTR
STTPAAQKEQPKGGHPGGHPGGHPGGAPHGMGGGHGGGRPHGMGNPHGGGHPGKGHP
>Mature_537_residues
MRRPLPHRNFRKAMIPVTVFKDRTVALFGLGGSGLATAEALKAGGAHVIAYDDAEASRRKAGEAGILVQDLRSIDWTIVA
ALVLSPGVPLTHPEPHWSVKLARDAGAEVIGDIELFCRERHAIARRSPFIAITGTNGKSTTTALITHILRVAGRDVQMGG
NIGTAILSLAPPKPGRVHVVECSSYQVDLAPSLDPSVGVMLNITPDHLDRHGTIEHYAAVKERLVAGVEGGGTAVVGVDD
DFSAAMADRAVEAGKHVVRISVKRALPDGIWLDGETLVVSEGGAERFRLPLAGIGSLRGTHNAQNAAAAFAACRGVGVAP
EIITIAMKRFPGLAHRMEEVGNKGAVLFVNDSKATNADAAERALASFTSIFWIAGGKPKTGGIAPLAPFFDRVAKAYLIG
EAAEEFATTLGSTVAHEIAGTLDKAVAAAARDAEASGLKDPVVLLSPACASFDQFPNFEVRGDAFRELVKQVPGVEARTR
STTPAAQKEQPKGGHPGGHPGGHPGGAPHGMGGGHGGGRPHGMGNPHGGGHPGKGHP

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=473, Percent_Identity=33.1923890063425, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013116
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF07991 IlvN; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 55709; Mature: 55709

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPLPHRNFRKAMIPVTVFKDRTVALFGLGGSGLATAEALKAGGAHVIAYDDAEASRRK
CCCCCCCCCCHHHEEEEEEECCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHH
AGEAGILVQDLRSIDWTIVAALVLSPGVPLTHPEPHWSVKLARDAGAEVIGDIELFCRER
CCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
HAIARRSPFIAITGTNGKSTTTALITHILRVAGRDVQMGGNIGTAILSLAPPKPGRVHVV
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHEEECCCCCCEEEEE
ECSSYQVDLAPSLDPSVGVMLNITPDHLDRHGTIEHYAAVKERLVAGVEGGGTAVVGVDD
EECCEEEEECCCCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCC
DFSAAMADRAVEAGKHVVRISVKRALPDGIWLDGETLVVSEGGAERFRLPLAGIGSLRGT
CHHHHHHHHHHHCCCEEEEEEEHHCCCCCEEECCCEEEEECCCCCCEECCHHHCCCCCCC
HNAQNAAAAFAACRGVGVAPEIITIAMKRFPGLAHRMEEVGNKGAVLFVNDSKATNADAA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHHH
ERALASFTSIFWIAGGKPKTGGIAPLAPFFDRVAKAYLIGEAAEEFATTLGSTVAHEIAG
HHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLDKAVAAAARDAEASGLKDPVVLLSPACASFDQFPNFEVRGDAFRELVKQVPGVEARTR
HHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHCCCCEECCHHHHHHHHHCCCCCCCCC
STTPAAQKEQPKGGHPGGHPGGHPGGAPHGMGGGHGGGRPHGMGNPHGGGHPGKGHP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRPLPHRNFRKAMIPVTVFKDRTVALFGLGGSGLATAEALKAGGAHVIAYDDAEASRRK
CCCCCCCCCCHHHEEEEEEECCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHH
AGEAGILVQDLRSIDWTIVAALVLSPGVPLTHPEPHWSVKLARDAGAEVIGDIELFCRER
CCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
HAIARRSPFIAITGTNGKSTTTALITHILRVAGRDVQMGGNIGTAILSLAPPKPGRVHVV
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHEEECCCCCCEEEEE
ECSSYQVDLAPSLDPSVGVMLNITPDHLDRHGTIEHYAAVKERLVAGVEGGGTAVVGVDD
EECCEEEEECCCCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCC
DFSAAMADRAVEAGKHVVRISVKRALPDGIWLDGETLVVSEGGAERFRLPLAGIGSLRGT
CHHHHHHHHHHHCCCEEEEEEEHHCCCCCEEECCCEEEEECCCCCCEECCHHHCCCCCCC
HNAQNAAAAFAACRGVGVAPEIITIAMKRFPGLAHRMEEVGNKGAVLFVNDSKATNADAA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHHH
ERALASFTSIFWIAGGKPKTGGIAPLAPFFDRVAKAYLIGEAAEEFATTLGSTVAHEIAG
HHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLDKAVAAAARDAEASGLKDPVVLLSPACASFDQFPNFEVRGDAFRELVKQVPGVEARTR
HHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHCCCCEECCHHHHHHHHHCCCCCCCCC
STTPAAQKEQPKGGHPGGHPGGHPGGAPHGMGGGHGGGRPHGMGNPHGGGHPGKGHP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]