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The map label for this gene is rho

Identifier: 154245768

GI number: 154245768

Start: 2050819

End: 2052087

Strand: Reverse

Name: rho

Synonym: Xaut_1824

Alternate gene names: 154245768

Gene position: 2052087-2050819 (Counterclockwise)

Preceding gene: 154245769

Following gene: 154245767

Centisome position: 38.65

GC content: 62.49

Gene sequence:

>1269_bases
ATGCGGGAAGTGAAACTTCAAGACCTTAAGTCCAAGACCCCCGTGGAGCTCCTCGCCTTTGCCGAGGAGAGCCAGGTGGA
AAACGCCAGCACCCTGCGCAAGCAGGAGCTCATGTTTGCCATTCTGAAGCAGCTGGCGGCCACCGAAACCGAGATCATTG
GCGAAGGCGTCGTCGAGGTGCTCCAGGACGGCTTCGGCTTCCTGCGTTCGCCCGAGGCGAACTATCTGCCGGGACCGGAC
GACATTTATGTCTCCCCGTCCCAGATCCGGCGCTTCGGCCTGCGCACCGGCGACACGGTGGAAGGACAGATCCGCTCGCC
GAAGGAAGGCGAGCGCTATTTCGCGCTTCTCAAGGTCAATACGATCAATTTCGAAGACCCCGAGAAGACGCGCCACAAGA
TCAACTTCGACAACCTGACCCCGCTCTATCCCGACGAGCGCCTCAAGCTGGAGCATGAGGAAACCGTTGGGAAGAAGGAC
TTCTCGCCGCGAATCATCGATATCGTGGCGCCCATCGGCAAGGGCCAGCGCGGCCTCATCGTGGCCCCGCCTCGCACCGG
CAAGACGGTGCTGCTGCAGAACATCGCCAAGTCCATCACCGCCAACCATCCCGAATGCTTCCTCATCGTGCTGCTCATCG
ACGAGCGGCCGGAAGAAGTCACGGACATGCAGCGCTCGGTGAAGGGCGAGGTGGTGTCCTCCACCTTCGACGAGCCGGCC
ACCCGCCATGTGCAGGTCGCCGAGATGGTGATCGAGAAGGCCAAGCGGCTGGTGGAGCACGGGCGCGACGTGGTGATCCT
GCTGGATTCCATCACTCGTCTCGGCCGCGCCTACAACACGGTGGTGCCGTCCTCCGGCAAGGTGCTCACCGGCGGCGTGG
ACGCCAACGCCCTCCAGCGTCCCAAGCGCTTCTTCGGTGCGGCGCGTAACATCGAAGAAGGCGGCTCCCTCACCATCATC
GCCACCGCGCTCATCGAGACCGGCTCGCGCATGGACGAGGTGATCTTCGAGGAGTTCAAGGGCACCGGTAACTCGGAAGT
CATCCTCGACCGCAAGGTCTCCGACAAGCGCGTGTTCCCGGCCATCGACATCACCCGCTCGGGTACCCGCAAGGAAGAGC
TTCTGGTGCCGGCCGACACGCTCAAGAAGATGTACGTGCTGCGCCGCATCCTCAATCCCATGGGCACGGTGGACGCCATC
GAGTTCCTGCTCGACAAGCTGCGCCAGACCAAGACCAACGCGGACTTCTTCGATTCCATGAACACCTGA

Upstream 100 bases:

>100_bases
GCTTCCCCTGAAGACCTGCGACCCCGTCCCTCCCCCGCCGCCCATGCCTGATCGCTGCGATCGGCCTTGGCGTACTCAAG
ATTCATCGAGGCTTTCCCTT

Downstream 100 bases:

>100_bases
GGTCGCCCGCGTCCTCCACGGTGGTGCAAGATTCAGCCGTCGCGCCTTTTGGGCGTGGCGGCTGTGCTGTTTCAGGTACC
TGAACAAAGCGCCAACAGGG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MREVKLQDLKSKTPVELLAFAEESQVENASTLRKQELMFAILKQLAATETEIIGEGVVEVLQDGFGFLRSPEANYLPGPD
DIYVSPSQIRRFGLRTGDTVEGQIRSPKEGERYFALLKVNTINFEDPEKTRHKINFDNLTPLYPDERLKLEHEETVGKKD
FSPRIIDIVAPIGKGQRGLIVAPPRTGKTVLLQNIAKSITANHPECFLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPA
TRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTII
ATALIETGSRMDEVIFEEFKGTGNSEVILDRKVSDKRVFPAIDITRSGTRKEELLVPADTLKKMYVLRRILNPMGTVDAI
EFLLDKLRQTKTNADFFDSMNT

Sequences:

>Translated_422_residues
MREVKLQDLKSKTPVELLAFAEESQVENASTLRKQELMFAILKQLAATETEIIGEGVVEVLQDGFGFLRSPEANYLPGPD
DIYVSPSQIRRFGLRTGDTVEGQIRSPKEGERYFALLKVNTINFEDPEKTRHKINFDNLTPLYPDERLKLEHEETVGKKD
FSPRIIDIVAPIGKGQRGLIVAPPRTGKTVLLQNIAKSITANHPECFLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPA
TRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTII
ATALIETGSRMDEVIFEEFKGTGNSEVILDRKVSDKRVFPAIDITRSGTRKEELLVPADTLKKMYVLRRILNPMGTVDAI
EFLLDKLRQTKTNADFFDSMNT
>Mature_422_residues
MREVKLQDLKSKTPVELLAFAEESQVENASTLRKQELMFAILKQLAATETEIIGEGVVEVLQDGFGFLRSPEANYLPGPD
DIYVSPSQIRRFGLRTGDTVEGQIRSPKEGERYFALLKVNTINFEDPEKTRHKINFDNLTPLYPDERLKLEHEETVGKKD
FSPRIIDIVAPIGKGQRGLIVAPPRTGKTVLLQNIAKSITANHPECFLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPA
TRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTII
ATALIETGSRMDEVIFEEFKGTGNSEVILDRKVSDKRVFPAIDITRSGTRKEELLVPADTLKKMYVLRRILNPMGTVDAI
EFLLDKLRQTKTNADFFDSMNT

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=417, Percent_Identity=69.0647482014388, Blast_Score=606, Evalue=1e-175,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47204; Mature: 47204

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVKLQDLKSKTPVELLAFAEESQVENASTLRKQELMFAILKQLAATETEIIGEGVVEV
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQDGFGFLRSPEANYLPGPDDIYVSPSQIRRFGLRTGDTVEGQIRSPKEGERYFALLKVN
HHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
TINFEDPEKTRHKINFDNLTPLYPDERLKLEHEETVGKKDFSPRIIDIVAPIGKGQRGLI
EECCCCCHHHHHCCCCCCCCCCCCCHHEECCHHHHCCCCCCCCCEEEEEECCCCCCCCEE
VAPPRTGKTVLLQNIAKSITANHPECFLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPA
EECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCH
TRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQR
HHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHH
PKRFFGAARNIEEGGSLTIIATALIETGSRMDEVIFEEFKGTGNSEVILDRKVSDKRVFP
HHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
AIDITRSGTRKEELLVPADTLKKMYVLRRILNPMGTVDAIEFLLDKLRQTKTNADFFDSM
EEECCCCCCCCHHEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHCC
NT
CC
>Mature Secondary Structure
MREVKLQDLKSKTPVELLAFAEESQVENASTLRKQELMFAILKQLAATETEIIGEGVVEV
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQDGFGFLRSPEANYLPGPDDIYVSPSQIRRFGLRTGDTVEGQIRSPKEGERYFALLKVN
HHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
TINFEDPEKTRHKINFDNLTPLYPDERLKLEHEETVGKKDFSPRIIDIVAPIGKGQRGLI
EECCCCCHHHHHCCCCCCCCCCCCCHHEECCHHHHCCCCCCCCCEEEEEECCCCCCCCEE
VAPPRTGKTVLLQNIAKSITANHPECFLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPA
EECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCH
TRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQR
HHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHH
PKRFFGAARNIEEGGSLTIIATALIETGSRMDEVIFEEFKGTGNSEVILDRKVSDKRVFP
HHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
AIDITRSGTRKEELLVPADTLKKMYVLRRILNPMGTVDAIEFLLDKLRQTKTNADFFDSM
EEECCCCCCCCHHEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHCC
NT
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]