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The map label for this gene is metK [H]

Identifier: 154245760

GI number: 154245760

Start: 2044279

End: 2045472

Strand: Direct

Name: metK [H]

Synonym: Xaut_1816

Alternate gene names: 154245760

Gene position: 2044279-2045472 (Clockwise)

Preceding gene: 154245759

Following gene: 154245761

Centisome position: 38.51

GC content: 65.91

Gene sequence:

>1194_bases
ATGGCGCGTCAGTCCTATCTGTTCACCAGTGAGTCGGTTTCCGAAGGCCACCCTGACAAGGTCTGCGACCGCATTTCCGA
CGAAGTGGTGGATACCTTTTTCCGAACGGCCGAAGTCGAGGGCTGGGACCCCAGCCAGATCCGTGTGGCGTGCGAGACGC
TCGCCACCACCAACAAGGTGGTGATCGCCGGCGAGACCCGCGGCCCCTCTTCCATCACCCGCGACCTGCTGTCCCATGCT
GCCCGCCTTGCCATCAAGTCGATTGGCTATGAACAGCAGGGCTTCCACTGGGAAACGGCGGACATCGATGTCCTGCTGCA
TGCCCAGTCGGCCGACATTGCCCAGGGCGTCGACATCGCCGGCAACAAGGACGAAGGCGCCGGCGACCAGGGCATCATGT
TCGGCTATGCCGTGCGCGAAACGCCGGAGCTGATGCCTGCCCCGATCTACTACGCGCACAAGATCCTCAAGACCCTGGCC
GAAGCCCGCCATTCCGGCGAGACTCACGCCCTCGGCCCCGACGCCAAGAGCCAGGTCACCGTTCGCTACGAGGGCGGCAA
GCCGGTTGGCGTCACCCAGATCGTGCTGTCCACCCAGCACCTGGATGAGAACCTGTCGTCCCACGACGTGAAGTCCATCG
TTGAGCCCTATATCAGCGCCACGCTGCCCGAGGGCTGGATCTCCAAGGACACGGTCTGGCACGTCAATCCCACCGGCAAG
TTCGTGATCGGCGGGCCGGACGGCGACTGCGGCCTTACCGGGCGCAAGATCATCGTGGACACCTACGGCGGCGCGGCTCC
CCACGGCGGTGGCGCCTTCTCCGGCAAGGATCCCACCAAGGTCGACCGCTCCGCGGCCTATGCCGCGCGCTACCTCGCCA
AGAACGTGGTTGCGGCTGGCCTCGCGGACCGCGCCACCATCCAGCTGGCCTATGCCATCGGCGTGTCCGACCCGCTGGCG
GTCTATGTGGACCTGCACGGCACCGGCAACGTGGACGAGGCCAAGCTCGAGACCGTGCTGCGGGAAGTGATGAACCTGCG
TCCCCGCGGCATTCGCGAGCATCTGGGCCTGACCAAGCCCATCTATGCCCGCACCGCCGCCTATGGTCATTTCGGCCGCG
CGCCGGAAGCGGATGGCGGCTTCTCCTGGGAGAAGACCGACCTCGTGGACGCCCTCAAGTCGGCGGTCGCCTGA

Upstream 100 bases:

>100_bases
ATTTCGCGCTACCGACCTTGACCGCTCGCTGCCGCGTTGCCACAAGAAGAGACCGCAACGCGGCGCGGGCGCAAGAGGGT
CGGAGAAGAGGGTCGAAGAC

Downstream 100 bases:

>100_bases
CGCCAGACCACGCGGTCGCCCGCCCGGACCTCCGGGCGGCGCGCTGGATGGAACTGCGCTGTGATGGAGCGAAGGGGTCC
CGGATGCCACCGCATCGCCG

Product: methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MARQSYLFTSESVSEGHPDKVCDRISDEVVDTFFRTAEVEGWDPSQIRVACETLATTNKVVIAGETRGPSSITRDLLSHA
ARLAIKSIGYEQQGFHWETADIDVLLHAQSADIAQGVDIAGNKDEGAGDQGIMFGYAVRETPELMPAPIYYAHKILKTLA
EARHSGETHALGPDAKSQVTVRYEGGKPVGVTQIVLSTQHLDENLSSHDVKSIVEPYISATLPEGWISKDTVWHVNPTGK
FVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLADRATIQLAYAIGVSDPLA
VYVDLHGTGNVDEAKLETVLREVMNLRPRGIREHLGLTKPIYARTAAYGHFGRAPEADGGFSWEKTDLVDALKSAVA

Sequences:

>Translated_397_residues
MARQSYLFTSESVSEGHPDKVCDRISDEVVDTFFRTAEVEGWDPSQIRVACETLATTNKVVIAGETRGPSSITRDLLSHA
ARLAIKSIGYEQQGFHWETADIDVLLHAQSADIAQGVDIAGNKDEGAGDQGIMFGYAVRETPELMPAPIYYAHKILKTLA
EARHSGETHALGPDAKSQVTVRYEGGKPVGVTQIVLSTQHLDENLSSHDVKSIVEPYISATLPEGWISKDTVWHVNPTGK
FVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLADRATIQLAYAIGVSDPLA
VYVDLHGTGNVDEAKLETVLREVMNLRPRGIREHLGLTKPIYARTAAYGHFGRAPEADGGFSWEKTDLVDALKSAVA
>Mature_396_residues
ARQSYLFTSESVSEGHPDKVCDRISDEVVDTFFRTAEVEGWDPSQIRVACETLATTNKVVIAGETRGPSSITRDLLSHAA
RLAIKSIGYEQQGFHWETADIDVLLHAQSADIAQGVDIAGNKDEGAGDQGIMFGYAVRETPELMPAPIYYAHKILKTLAE
ARHSGETHALGPDAKSQVTVRYEGGKPVGVTQIVLSTQHLDENLSSHDVKSIVEPYISATLPEGWISKDTVWHVNPTGKF
VIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLADRATIQLAYAIGVSDPLAV
YVDLHGTGNVDEAKLETVLREVMNLRPRGIREHLGLTKPIYARTAAYGHFGRAPEADGGFSWEKTDLVDALKSAVA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=387, Percent_Identity=48.8372093023256, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI4557737, Length=387, Percent_Identity=48.062015503876, Blast_Score=319, Evalue=3e-87,
Organism=Escherichia coli, GI1789311, Length=392, Percent_Identity=51.7857142857143, Blast_Score=380, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17538498, Length=393, Percent_Identity=48.3460559796438, Blast_Score=327, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17538494, Length=393, Percent_Identity=48.0916030534351, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI25145633, Length=393, Percent_Identity=48.3460559796438, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=45.2196382428941, Blast_Score=300, Evalue=8e-82,
Organism=Caenorhabditis elegans, GI32565905, Length=391, Percent_Identity=45.0127877237852, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI32565911, Length=334, Percent_Identity=47.9041916167665, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI25145635, Length=334, Percent_Identity=47.9041916167665, Blast_Score=267, Evalue=9e-72,
Organism=Caenorhabditis elegans, GI32565903, Length=300, Percent_Identity=49.6666666666667, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI32565909, Length=91, Percent_Identity=42.8571428571429, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=44.8717948717949, Blast_Score=308, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6323209, Length=389, Percent_Identity=44.987146529563, Blast_Score=300, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24580527, Length=391, Percent_Identity=46.8030690537084, Blast_Score=307, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24580525, Length=391, Percent_Identity=46.8030690537084, Blast_Score=307, Evalue=8e-84,
Organism=Drosophila melanogaster, GI45552159, Length=389, Percent_Identity=46.2724935732648, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24580529, Length=389, Percent_Identity=46.2724935732648, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24580537, Length=389, Percent_Identity=46.2724935732648, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24580533, Length=389, Percent_Identity=46.2724935732648, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24580535, Length=389, Percent_Identity=46.2724935732648, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24580531, Length=389, Percent_Identity=46.2724935732648, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24580541, Length=389, Percent_Identity=44.7300771208226, Blast_Score=284, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24580539, Length=131, Percent_Identity=40.4580152671756, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42563; Mature: 42432

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQSYLFTSESVSEGHPDKVCDRISDEVVDTFFRTAEVEGWDPSQIRVACETLATTNKV
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEE
VIAGETRGPSSITRDLLSHAARLAIKSIGYEQQGFHWETADIDVLLHAQSADIAQGVDIA
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEEECCCCHHCCCCCC
GNKDEGAGDQGIMFGYAVRETPELMPAPIYYAHKILKTLAEARHSGETHALGPDAKSQVT
CCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
VRYEGGKPVGVTQIVLSTQHLDENLSSHDVKSIVEPYISATLPEGWISKDTVWHVNPTGK
EEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
FVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAG
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LADRATIQLAYAIGVSDPLAVYVDLHGTGNVDEAKLETVLREVMNLRPRGIREHLGLTKP
CCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCC
IYARTAAYGHFGRAPEADGGFSWEKTDLVDALKSAVA
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ARQSYLFTSESVSEGHPDKVCDRISDEVVDTFFRTAEVEGWDPSQIRVACETLATTNKV
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEE
VIAGETRGPSSITRDLLSHAARLAIKSIGYEQQGFHWETADIDVLLHAQSADIAQGVDIA
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEEECCCCHHCCCCCC
GNKDEGAGDQGIMFGYAVRETPELMPAPIYYAHKILKTLAEARHSGETHALGPDAKSQVT
CCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
VRYEGGKPVGVTQIVLSTQHLDENLSSHDVKSIVEPYISATLPEGWISKDTVWHVNPTGK
EEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
FVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAG
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LADRATIQLAYAIGVSDPLAVYVDLHGTGNVDEAKLETVLREVMNLRPRGIREHLGLTKP
CCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCC
IYARTAAYGHFGRAPEADGGFSWEKTDLVDALKSAVA
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA