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The map label for this gene is trmB

Identifier: 154245761

GI number: 154245761

Start: 2045587

End: 2046282

Strand: Direct

Name: trmB

Synonym: Xaut_1817

Alternate gene names: 154245761

Gene position: 2045587-2046282 (Clockwise)

Preceding gene: 154245760

Following gene: 154245771

Centisome position: 38.53

GC content: 69.4

Gene sequence:

>696_bases
ATGCAGGACATGACCGAAGAAACGGAACGTCACGGCGCCTTCTATGGCCGGCGCATCGGCAAGTCGCTGCGTGGCGCCCA
GCAGGACGCCATGGTGCGCCTCCTGCCCATCTGGCGCTTCGCCGCCGAGGACGCACGCACCCCGGCCTCCCTGTTCCCGC
ACGACATCAGTGGTCTGTGGCTGGAGATCGGCTTCGGCGGCGGGGAGCATCTCATCTCCCATGCCACGCGCCGGCCCGAT
ATGGGCTTCATCGGCGCCGAGCCGTTCGTGAACGGCATGGCGCGCATGCTGGTGGACTTGGAGAAGGCCGGGCTTTCCAA
CATCCGCCTGTTCGGCGAGGACGCGGCATTGCTGCTGGATCGCCTGCCGGAGGCGAGCCTCGACGGCGCCGACCTGTTTT
ATCCCGACCCCTGGCCCAAGCGCCGCCACTGGAAGCGCCGGTTCGTCCGGCCGGACAATCTGGACCGCCTCGCCCGCGCC
ATCCGCCCGGGCGGCGTGTTCCGCTTCGCATCCGACGTGCCGCACTATGTGGGCTGGACCCTGGCCGAAATGCACGCCCA
CGGTGCCTTCCGCTGGACCGCCGAGCGGGCCGACGACTGGCGCCAGCCCTATCCGCACTGGCCCGGCACCCGCTACGAGG
CGAAGGCCCTGGAGGAAGGGCGCAGCCCGACCTACCTGACCTTCGAGCGGATCTGA

Upstream 100 bases:

>100_bases
GTCGCCCGCCCGGACCTCCGGGCGGCGCGCTGGATGGAACTGCGCTGTGATGGAGCGAAGGGGTCCCGGATGCCACCGCA
TCGCCGGGATCCGTCATGAC

Downstream 100 bases:

>100_bases
TCCGGTCGCTTCAGGCCGCGCCAGACCCCTCAGGCGCCGGAATCGTAAAGCGCCTTAAGGCCCTCACGATAGGTGGGATA
GGCGAGGCTCACCCCCAGTT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MQDMTEETERHGAFYGRRIGKSLRGAQQDAMVRLLPIWRFAAEDARTPASLFPHDISGLWLEIGFGGGEHLISHATRRPD
MGFIGAEPFVNGMARMLVDLEKAGLSNIRLFGEDAALLLDRLPEASLDGADLFYPDPWPKRRHWKRRFVRPDNLDRLARA
IRPGGVFRFASDVPHYVGWTLAEMHAHGAFRWTAERADDWRQPYPHWPGTRYEAKALEEGRSPTYLTFERI

Sequences:

>Translated_231_residues
MQDMTEETERHGAFYGRRIGKSLRGAQQDAMVRLLPIWRFAAEDARTPASLFPHDISGLWLEIGFGGGEHLISHATRRPD
MGFIGAEPFVNGMARMLVDLEKAGLSNIRLFGEDAALLLDRLPEASLDGADLFYPDPWPKRRHWKRRFVRPDNLDRLARA
IRPGGVFRFASDVPHYVGWTLAEMHAHGAFRWTAERADDWRQPYPHWPGTRYEAKALEEGRSPTYLTFERI
>Mature_231_residues
MQDMTEETERHGAFYGRRIGKSLRGAQQDAMVRLLPIWRFAAEDARTPASLFPHDISGLWLEIGFGGGEHLISHATRRPD
MGFIGAEPFVNGMARMLVDLEKAGLSNIRLFGEDAALLLDRLPEASLDGADLFYPDPWPKRRHWKRRFVRPDNLDRLARA
IRPGGVFRFASDVPHYVGWTLAEMHAHGAFRWTAERADDWRQPYPHWPGTRYEAKALEEGRSPTYLTFERI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=219, Percent_Identity=32.4200913242009, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26510; Mature: 26510

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDMTEETERHGAFYGRRIGKSLRGAQQDAMVRLLPIWRFAAEDARTPASLFPHDISGLW
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEE
LEIGFGGGEHLISHATRRPDMGFIGAEPFVNGMARMLVDLEKAGLSNIRLFGEDAALLLD
EEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
RLPEASLDGADLFYPDPWPKRRHWKRRFVRPDNLDRLARAIRPGGVFRFASDVPHYVGWT
HCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEHHCCCHHHHHH
LAEMHAHGAFRWTAERADDWRQPYPHWPGTRYEAKALEEGRSPTYLTFERI
HHHHHHCCCEEEEHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MQDMTEETERHGAFYGRRIGKSLRGAQQDAMVRLLPIWRFAAEDARTPASLFPHDISGLW
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEE
LEIGFGGGEHLISHATRRPDMGFIGAEPFVNGMARMLVDLEKAGLSNIRLFGEDAALLLD
EEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
RLPEASLDGADLFYPDPWPKRRHWKRRFVRPDNLDRLARAIRPGGVFRFASDVPHYVGWT
HCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEHHCCCHHHHHH
LAEMHAHGAFRWTAERADDWRQPYPHWPGTRYEAKALEEGRSPTYLTFERI
HHHHHHCCCEEEEHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA