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The map label for this gene is paaF [C]

Identifier: 154245644

GI number: 154245644

Start: 1914650

End: 1915456

Strand: Reverse

Name: paaF [C]

Synonym: Xaut_1700

Alternate gene names: 154245644

Gene position: 1915456-1914650 (Counterclockwise)

Preceding gene: 154245645

Following gene: 154245642

Centisome position: 36.08

GC content: 66.29

Gene sequence:

>807_bases
ATGAAGCGGATCCCCCTGGAAGAGCGCTACGAGACCCTGAAGATCGAGCGCAAGGGCCGGCTCTTGACCCTCACCATGAA
CCGCCCGGATTTCCTCAACGCGGCCGACAAGGTCATGCATGAGGAATTGTCCGAGGTGTTCTACGACATCGCCAGCGATC
CCGGCAGCGACGTGGTGGTGTTCACCGGGGCGGGACGGGCCTTCTCCTCCGGCGGCGACATCAACTGGATGCAGGAGATG
ATCGACCATCCCCACACCTTCGAGCAGACGGCGCGGGAGGCCAAGCGCATCGTCTTCTCCATGCTGGATCTGGAGAAGCC
GCTCATCGCCCGCGTCAACGGCCACGCCACCGGCCTTGGGGCGACGCTGGCCCTGATGTGCGATGTGGTCTTCGCCGCGT
CGGACGCCAAGATCGGCGATCCCCATGTGGCGGTTGGCTTCGTCGCTGGCGATGGTGGCGCCGCCATCTGGCCGCAGCTC
ATCGGCTATGCGCGGGCCAAGGAATTCCTCATGAGCGGCGACCTCTTGACCGCCCCGCGCGCGGCCGAGATCGGCCTCAT
CAACCACGCCGTTCCCGCCGCCGAGCTGGACGCGGCGGTGGAGGCCTATGTGGGCAAGCTGCTGGCGGGCGCCACCAAGG
CCATCCGCTGGTCGAAGGTGGCCACCAACATCGAGCTGAAGCGCATCGTCCATGGCGTCATGGACGCGAGCCTCGCCTAT
GAGGCCCTGTCCAACATCACCGCCGACCACCAGGAGGCGGTGAACGCCTTCCGCGACAAACGCAAGCCCGGCTTCTCCGG
CCGCTGA

Upstream 100 bases:

>100_bases
TGCGGCGCTGTTCGGCTCCACGCAGGAGCATCTCGCCCGCTTCGGCGTCGCCGCGTGAAGTCTTCAACACACCTTCAAGA
CGAAGAAAGACCTTTCTCCC

Downstream 100 bases:

>100_bases
GGAATTGGGGGCGACAACGCCCCCAATCAATGCAGCGCGTGCGCGGTGGGCGGCTCGGTGTCGTGCAGCAGGCCATAGAG
ATTGCGGAAGATGTGGCAGT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKRIPLEERYETLKIERKGRLLTLTMNRPDFLNAADKVMHEELSEVFYDIASDPGSDVVVFTGAGRAFSSGGDINWMQEM
IDHPHTFEQTAREAKRIVFSMLDLEKPLIARVNGHATGLGATLALMCDVVFAASDAKIGDPHVAVGFVAGDGGAAIWPQL
IGYARAKEFLMSGDLLTAPRAAEIGLINHAVPAAELDAAVEAYVGKLLAGATKAIRWSKVATNIELKRIVHGVMDASLAY
EALSNITADHQEAVNAFRDKRKPGFSGR

Sequences:

>Translated_268_residues
MKRIPLEERYETLKIERKGRLLTLTMNRPDFLNAADKVMHEELSEVFYDIASDPGSDVVVFTGAGRAFSSGGDINWMQEM
IDHPHTFEQTAREAKRIVFSMLDLEKPLIARVNGHATGLGATLALMCDVVFAASDAKIGDPHVAVGFVAGDGGAAIWPQL
IGYARAKEFLMSGDLLTAPRAAEIGLINHAVPAAELDAAVEAYVGKLLAGATKAIRWSKVATNIELKRIVHGVMDASLAY
EALSNITADHQEAVNAFRDKRKPGFSGR
>Mature_268_residues
MKRIPLEERYETLKIERKGRLLTLTMNRPDFLNAADKVMHEELSEVFYDIASDPGSDVVVFTGAGRAFSSGGDINWMQEM
IDHPHTFEQTAREAKRIVFSMLDLEKPLIARVNGHATGLGATLALMCDVVFAASDAKIGDPHVAVGFVAGDGGAAIWPQL
IGYARAKEFLMSGDLLTAPRAAEIGLINHAVPAAELDAAVEAYVGKLLAGATKAIRWSKVATNIELKRIVHGVMDASLAY
EALSNITADHQEAVNAFRDKRKPGFSGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=263, Percent_Identity=28.5171102661597, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=32.0224719101124, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI45643119, Length=228, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI260275230, Length=228, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI260274832, Length=228, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI70995211, Length=206, Percent_Identity=28.6407766990291, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI150378541, Length=255, Percent_Identity=30.9803921568627, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI116235487, Length=203, Percent_Identity=25.1231527093596, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757966, Length=163, Percent_Identity=31.2883435582822, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538581, Length=163, Percent_Identity=31.2883435582822, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI25453481, Length=163, Percent_Identity=31.2883435582822, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538573, Length=163, Percent_Identity=31.2883435582822, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757968, Length=163, Percent_Identity=31.2883435582822, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI49355787, Length=163, Percent_Identity=31.2883435582822, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI221307494, Length=171, Percent_Identity=30.9941520467836, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221136756, Length=209, Percent_Identity=27.7511961722488, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221136753, Length=209, Percent_Identity=27.7511961722488, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=25.9842519685039, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4502327, Length=267, Percent_Identity=28.4644194756554, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI68989263, Length=179, Percent_Identity=28.4916201117318, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=34.7169811320755, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788597, Length=266, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=32.9365079365079, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=29.6577946768061, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=24.1509433962264, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788682, Length=150, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=28.9473684210526, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=30.7692307692308, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17554946, Length=267, Percent_Identity=29.9625468164794, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=209, Percent_Identity=29.6650717703349, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=205, Percent_Identity=28.2926829268293, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=188, Percent_Identity=30.3191489361702, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=193, Percent_Identity=25.3886010362694, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=205, Percent_Identity=25.3658536585366, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129971, Length=267, Percent_Identity=29.9625468164794, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24653477, Length=267, Percent_Identity=29.9625468164794, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24654903, Length=191, Percent_Identity=30.3664921465969, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=29.6577946768061, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=24.9027237354086, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922422, Length=245, Percent_Identity=24.4897959183673, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24650670, Length=189, Percent_Identity=26.4550264550265, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29059; Mature: 29059

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIPLEERYETLKIERKGRLLTLTMNRPDFLNAADKVMHEELSEVFYDIASDPGSDVVV
CCCCCHHHHHHHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
FTGAGRAFSSGGDINWMQEMIDHPHTFEQTAREAKRIVFSMLDLEKPLIARVNGHATGLG
EECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHH
ATLALMCDVVFAASDAKIGDPHVAVGFVAGDGGAAIWPQLIGYARAKEFLMSGDLLTAPR
HHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
AAEIGLINHAVPAAELDAAVEAYVGKLLAGATKAIRWSKVATNIELKRIVHGVMDASLAY
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EALSNITADHQEAVNAFRDKRKPGFSGR
HHHHHCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRIPLEERYETLKIERKGRLLTLTMNRPDFLNAADKVMHEELSEVFYDIASDPGSDVVV
CCCCCHHHHHHHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
FTGAGRAFSSGGDINWMQEMIDHPHTFEQTAREAKRIVFSMLDLEKPLIARVNGHATGLG
EECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHH
ATLALMCDVVFAASDAKIGDPHVAVGFVAGDGGAAIWPQLIGYARAKEFLMSGDLLTAPR
HHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
AAEIGLINHAVPAAELDAAVEAYVGKLLAGATKAIRWSKVATNIELKRIVHGVMDASLAY
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EALSNITADHQEAVNAFRDKRKPGFSGR
HHHHHCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA