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The map label for this gene is nth [H]

Identifier: 154245577

GI number: 154245577

Start: 1849201

End: 1850280

Strand: Direct

Name: nth [H]

Synonym: Xaut_1632

Alternate gene names: 154245577

Gene position: 1849201-1850280 (Clockwise)

Preceding gene: 154245575

Following gene: 154245579

Centisome position: 34.83

GC content: 70.46

Gene sequence:

>1080_bases
ATGCCGCGCAAGATCACCCCGCCCAAACCTTCCGTTGCGACCGAGGCAGCAGCCGCCAAACCCGATGCCGCCACACCCGG
CGTTTCCGCACCGGCAAAGCCGCGTGCGGCGCCGGCATCTGCTTTACCGGCTGCGAAAGCCGGGACGGGGAGCGGCAAGG
CTGGAGCGAAGGACAAGGCCCGAGCAGGTGCGGCCAAGGGGGCTGCCAAGTCCCCGGCCAAGGGTGACGCCAAGGGTGGC
GCCAAGGGTGCGGCCCAAGCTACGGCCCAAGCTCCCGCCAAGGTTCCCGCCAAGGTTCCCGCCAAGGCTCCGGCCAAGCC
GGCCGCCAAATCCGCACCCTCCGGCAAGCGCAAGCCGGCCACGCCACCACCCGCCAGCCCCGCGCCCCGCCGCAACAAGG
CGGTGGCCAATGCCGCCGCCGCCGCCCACGGCAAGACGCTCCTCGTCTGGAGCGAAGAGGAGATCGCCGAGGCCTTCGCC
CGCTTCGAGGCGCAGGATCCCGAGCCCAAGGGCGAACTCAACCACACCGATGCCTTCACCCTGCTGGTTGCCGTGGTGCT
CTCGGCACAGGCCACCGATACGGGGGTCAACAAGGCCACCACCGGCCTGTTCGCTGCCGCCGCCACCCCCGCCGCCATGG
TCACGCTCGGCGAGGAGGAGGTGGCCCGCCGCATTCGCACCCTCGGCCTCTATCGCGGCAAGGCGAAAAATGTGGTGGAG
CTGTCCCGCCTGCTGCTGGAACGGCATGCCGGCATGGTGCCGCGCGACCGCGAGGCGCTGGAGGCGCTGCCGGGGGTGGG
GCGCAAGACCGCCAATGTGGTGCTGAACATCGCCTTCGGCGCGCCGACCATTGCGGTGGACACCCACCTGTTCCGGGTGG
CAAACCGCACCGGCCTAGCTCCCGGCCCGACGCCGCTGGCGGTGGAACTGGGCCTGGAGGCGCGCATCCCCGACCGCTTC
AAGCTGCATGCCCACCACTGGCTGATTCTGCACGGCCGCTACATCTGCAAGGCGTCCAGGCCCGAATGCGGCCGCTGCAT
CATCGCGGATCTCTGCCGCTGGCCGGAGAAACGCTTCTGA

Upstream 100 bases:

>100_bases
CACCGCATAGACGACGGGTTCCTCCGGGAACCCCGCGTCCCCTTCGGGTGCGGTATCACGGTCGCTGGCTTGGGGCATTG
GCGAACAGTATACCGGAAAA

Downstream 100 bases:

>100_bases
GGGGGCTCTCAGAGCCTCTTGAAGATCCGCCGCGATCGGTGTTTTCAGCCCTTTATCGGTATTTAAAACCGTCATGGCCG
GGCTTGTCCCGGCCATCCAC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPRKITPPKPSVATEAAAAKPDAATPGVSAPAKPRAAPASALPAAKAGTGSGKAGAKDKARAGAAKGAAKSPAKGDAKGG
AKGAAQATAQAPAKVPAKVPAKAPAKPAAKSAPSGKRKPATPPPASPAPRRNKAVANAAAAAHGKTLLVWSEEEIAEAFA
RFEAQDPEPKGELNHTDAFTLLVAVVLSAQATDTGVNKATTGLFAAAATPAAMVTLGEEEVARRIRTLGLYRGKAKNVVE
LSRLLLERHAGMVPRDREALEALPGVGRKTANVVLNIAFGAPTIAVDTHLFRVANRTGLAPGPTPLAVELGLEARIPDRF
KLHAHHWLILHGRYICKASRPECGRCIIADLCRWPEKRF

Sequences:

>Translated_359_residues
MPRKITPPKPSVATEAAAAKPDAATPGVSAPAKPRAAPASALPAAKAGTGSGKAGAKDKARAGAAKGAAKSPAKGDAKGG
AKGAAQATAQAPAKVPAKVPAKAPAKPAAKSAPSGKRKPATPPPASPAPRRNKAVANAAAAAHGKTLLVWSEEEIAEAFA
RFEAQDPEPKGELNHTDAFTLLVAVVLSAQATDTGVNKATTGLFAAAATPAAMVTLGEEEVARRIRTLGLYRGKAKNVVE
LSRLLLERHAGMVPRDREALEALPGVGRKTANVVLNIAFGAPTIAVDTHLFRVANRTGLAPGPTPLAVELGLEARIPDRF
KLHAHHWLILHGRYICKASRPECGRCIIADLCRWPEKRF
>Mature_358_residues
PRKITPPKPSVATEAAAAKPDAATPGVSAPAKPRAAPASALPAAKAGTGSGKAGAKDKARAGAAKGAAKSPAKGDAKGGA
KGAAQATAQAPAKVPAKVPAKAPAKPAAKSAPSGKRKPATPPPASPAPRRNKAVANAAAAAHGKTLLVWSEEEIAEAFAR
FEAQDPEPKGELNHTDAFTLLVAVVLSAQATDTGVNKATTGLFAAAATPAAMVTLGEEEVARRIRTLGLYRGKAKNVVEL
SRLLLERHAGMVPRDREALEALPGVGRKTANVVLNIAFGAPTIAVDTHLFRVANRTGLAPGPTPLAVELGLEARIPDRFK
LHAHHWLILHGRYICKASRPECGRCIIADLCRWPEKRF

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=29.3785310734463, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI190358497, Length=218, Percent_Identity=30.2752293577982, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI6912520, Length=226, Percent_Identity=30.0884955752212, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI115298654, Length=218, Percent_Identity=30.2752293577982, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI115298652, Length=218, Percent_Identity=30.2752293577982, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI115298650, Length=218, Percent_Identity=30.2752293577982, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI115298648, Length=226, Percent_Identity=30.0884955752212, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=59.7014925373134, Blast_Score=250, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.7683615819209, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=31.1111111111111, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 36954; Mature: 36823

Theoretical pI: Translated: 11.00; Mature: 11.00

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKITPPKPSVATEAAAAKPDAATPGVSAPAKPRAAPASALPAAKAGTGSGKAGAKDKA
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHH
RAGAAKGAAKSPAKGDAKGGAKGAAQATAQAPAKVPAKVPAKAPAKPAAKSAPSGKRKPA
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TPPPASPAPRRNKAVANAAAAAHGKTLLVWSEEEIAEAFARFEAQDPEPKGELNHTDAFT
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
LLVAVVLSAQATDTGVNKATTGLFAAAATPAAMVTLGEEEVARRIRTLGLYRGKAKNVVE
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCHHHH
LSRLLLERHAGMVPRDREALEALPGVGRKTANVVLNIAFGAPTIAVDTHLFRVANRTGLA
HHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCCCEEEHHHHHHHHHHCCCCC
PGPTPLAVELGLEARIPDRFKLHAHHWLILHGRYICKASRPECGRCIIADLCRWPEKRF
CCCCCEEEEECCCCCCCCCEEEEEEEEEEEECEEEEECCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PRKITPPKPSVATEAAAAKPDAATPGVSAPAKPRAAPASALPAAKAGTGSGKAGAKDKA
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHH
RAGAAKGAAKSPAKGDAKGGAKGAAQATAQAPAKVPAKVPAKAPAKPAAKSAPSGKRKPA
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TPPPASPAPRRNKAVANAAAAAHGKTLLVWSEEEIAEAFARFEAQDPEPKGELNHTDAFT
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
LLVAVVLSAQATDTGVNKATTGLFAAAATPAAMVTLGEEEVARRIRTLGLYRGKAKNVVE
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCHHHH
LSRLLLERHAGMVPRDREALEALPGVGRKTANVVLNIAFGAPTIAVDTHLFRVANRTGLA
HHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCCCEEEHHHHHHHHHHCCCCC
PGPTPLAVELGLEARIPDRFKLHAHHWLILHGRYICKASRPECGRCIIADLCRWPEKRF
CCCCCEEEEECCCCCCCCCEEEEEEEEEEEECEEEEECCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]