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The map label for this gene is lutR [H]

Identifier: 154245569

GI number: 154245569

Start: 1838364

End: 1839080

Strand: Direct

Name: lutR [H]

Synonym: Xaut_1624

Alternate gene names: 154245569

Gene position: 1838364-1839080 (Clockwise)

Preceding gene: 154245568

Following gene: 154245572

Centisome position: 34.63

GC content: 72.38

Gene sequence:

>717_bases
ATGGCCCAACCCCGCGACATCATCGGCCGGGCGCCCTCCCTCGCCGAGACGCTGGCGCACCGGCTCCGGGAGGAGATCGC
TTCCGGCCGCCTCCAGCCGGGCGAGCGACTGCCCACCGAGCACCAGATGGCGGAGACCTACGGCGTCAGCCGGCCCATCG
TGCGCGAGGCGGTGGGGCGGCTGAAGCATGACGGCCTCGTCACCACGCGCCAGGGCGCGGGCGCATTCGTGGCCGAGCCG
GGGGCAGCCTCCACCTTCCGGCTCGACATCGCCGATTTCAGCGACAAGGTGGAGATGCGCGCCATCGTGGAACTGCTGAT
GGCGGTGGAGGCCACCGCCACCGCCCATGCCGCCGTGCGCCGCTCGGAGGCGGATCTCGCCCGCATCGGGGAGCAGCTCG
ACGCCATGCAGGAGGCCGTCGACCGCGGCGAGCCGGGCGTGGACGAGGACATGGCCTTCCACCGCGCCATCGTGGAGGCG
ACGGGCAATCCCTATTTCCGCGACCTGTCCCAGTTTCTCGACCACCGGGTGCGCCATTTCATCCGCACCGCGCGGGCGAA
CTCGGCGCGGCTCGGCGGCTTGCCCCAGGCGGTGCAGCGCGAGCATGAGGCCATCTTCGCCGCCATCGAGAAGAAGGACC
CTGTCGCCGCCCGCGCCGCTGCCGAGGAGCACCTGCGCAACGCCGCCGCCCGGCTCGCGGTTTATCTGGGGCCGTAG

Upstream 100 bases:

>100_bases
CGTCACCGGCGAGACGGTCCACGTGAACGGTGGTCTTTATATTGCCAGTTGAGTCGCGCCATCTTGTGTGTGGCGCAGCC
GGCTGAGCTAGGAGCAACGG

Downstream 100 bases:

>100_bases
CGGGCTGGAGCATTTGATCGGCGTGGCGTGCAGCGGAGCCTTGTTGCGCTGCCGATGAGCCCCTTCCCGCCGTACGGGGG
AGGGCAGGGTGGGGGCTGGA

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MAQPRDIIGRAPSLAETLAHRLREEIASGRLQPGERLPTEHQMAETYGVSRPIVREAVGRLKHDGLVTTRQGAGAFVAEP
GAASTFRLDIADFSDKVEMRAIVELLMAVEATATAHAAVRRSEADLARIGEQLDAMQEAVDRGEPGVDEDMAFHRAIVEA
TGNPYFRDLSQFLDHRVRHFIRTARANSARLGGLPQAVQREHEAIFAAIEKKDPVAARAAAEEHLRNAAARLAVYLGP

Sequences:

>Translated_238_residues
MAQPRDIIGRAPSLAETLAHRLREEIASGRLQPGERLPTEHQMAETYGVSRPIVREAVGRLKHDGLVTTRQGAGAFVAEP
GAASTFRLDIADFSDKVEMRAIVELLMAVEATATAHAAVRRSEADLARIGEQLDAMQEAVDRGEPGVDEDMAFHRAIVEA
TGNPYFRDLSQFLDHRVRHFIRTARANSARLGGLPQAVQREHEAIFAAIEKKDPVAARAAAEEHLRNAAARLAVYLGP
>Mature_237_residues
AQPRDIIGRAPSLAETLAHRLREEIASGRLQPGERLPTEHQMAETYGVSRPIVREAVGRLKHDGLVTTRQGAGAFVAEPG
AASTFRLDIADFSDKVEMRAIVELLMAVEATATAHAAVRRSEADLARIGEQLDAMQEAVDRGEPGVDEDMAFHRAIVEAT
GNPYFRDLSQFLDHRVRHFIRTARANSARLGGLPQAVQREHEAIFAAIEKKDPVAARAAAEEHLRNAAARLAVYLGP

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=226, Percent_Identity=31.858407079646, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786303, Length=220, Percent_Identity=31.3636363636364, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994955, Length=217, Percent_Identity=29.9539170506912, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790032, Length=222, Percent_Identity=28.8288288288288, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI48994961, Length=230, Percent_Identity=29.1304347826087, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789621, Length=238, Percent_Identity=25.6302521008403, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25995; Mature: 25864

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPRDIIGRAPSLAETLAHRLREEIASGRLQPGERLPTEHQMAETYGVSRPIVREAVGR
CCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
LKHDGLVTTRQGAGAFVAEPGAASTFRLDIADFSDKVEMRAIVELLMAVEATATAHAAVR
HHHCCCEEECCCCCCEEECCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSEADLARIGEQLDAMQEAVDRGEPGVDEDMAFHRAIVEATGNPYFRDLSQFLDHRVRHF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IRTARANSARLGGLPQAVQREHEAIFAAIEKKDPVAARAAAEEHLRNAAARLAVYLGP
HHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
AQPRDIIGRAPSLAETLAHRLREEIASGRLQPGERLPTEHQMAETYGVSRPIVREAVGR
CCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
LKHDGLVTTRQGAGAFVAEPGAASTFRLDIADFSDKVEMRAIVELLMAVEATATAHAAVR
HHHCCCEEECCCCCCEEECCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSEADLARIGEQLDAMQEAVDRGEPGVDEDMAFHRAIVEATGNPYFRDLSQFLDHRVRHF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IRTARANSARLGGLPQAVQREHEAIFAAIEKKDPVAARAAAEEHLRNAAARLAVYLGP
HHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]