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The map label for this gene is mrpA [H]

Identifier: 154245527

GI number: 154245527

Start: 1792086

End: 1793777

Strand: Reverse

Name: mrpA [H]

Synonym: Xaut_1581

Alternate gene names: 154245527

Gene position: 1793777-1792086 (Counterclockwise)

Preceding gene: 154245528

Following gene: 154245526

Centisome position: 33.79

GC content: 70.57

Gene sequence:

>1692_bases
ATGACCGGCGAGACCCTGTCCCCGCCGCTCGATCTCTCCGCCCTGGAACCGGCCATGCCGGCGCTGTCCGAGGCGCTCAT
GGTGGTGGCGATCGCGCTGCCGCTGGTGCTGGCCTTCGCCGCGCTGGTGCCGGCGGTGCGACGCGCCCTGCCCCATCTTC
TGGTCTTCGTCCCGCTTCCGGCGCTGATCGCGGCCCTCGCCGTGCCGCGGGGACTGTCGATGCTGGTGGCGCCCGATCCC
TTCCGCTTCACCCTTGCCCTCGATGCGCCGGGCGCGCTGCTGCTGGGCGGGGCGTCGCTCTTGTGGATGACCGCCGGCAC
CTATGCCATGGCCTATCTGCGCAAGGACCCGGCGCGGCCGGCCTTCATGGTCTGGTGGCTGCTCACCATGGCGGGGTCCT
TCGCCGTGTTTCTGGTGGCGGACGTTGCGAGTTTCTATCTCGCCTATGCCCTGGTGAGCTTTTCCGCCTATGGCCTTGCC
GCCCATGACGATACCCCACAAGCCCGGCGCGCCGGCTCGCTCTACCTGGCCTTGACCGTGCTGGGCGAGGCGCTGCTCAT
CGCAGCCTTCGTCATGCTCGCCTCCGGCGCAACCGACGCCAACCCGCAGATCCGCGATGCGGTGGCCCTGCTGCCCACCT
CGCCTTGGAAGACGCCGGTGCTGGTTCTGATCCTCCTCGGCTTCATCCTGAAGATGGGCCTCTTTCCCCTCCATGTGTGG
ATGCCGCTGGCCCATTCCGTGGCGCCGGTTCCGGGCTCGGCGGTGCTCTCCGGCGTGGTGGTGAAGGCCGGGGTCATCGG
CCTTCTGCGCTTCCTGCCCGAGGGCGTGCCTTTACCCGGCTGGGGCGCGATGCTGTGCGGCGCCGGCCTGTTCACGGCCT
ATTACGGGGTCGCTGTGGGCTTCACCCAGACCCATCCCAAGCGCGCCCTCGCCTATTCCACCGTGAGCCAGATGGGCGTG
GTGGCGGCCGTGATCGGCGCCGGCGTCATGAACGGGACGGCCGGCACGTTCACCGTCGCCGCGCTTTATGCGGCGAACCA
CATGCTGCTGAAGGGCGCCATGTTCCTTGCTGTGGGTGTCGCCATGGCGTCCTCACCCAAGCGGGCATGGGCGGTGGCGG
CGCTCGCCGGCATGCTGGGCCTGAGTCTTGCCGGCCTGCCGCTGACCGGCGGCGCCATGGCGAAATACGCCATCAAGCTC
ATTGTGCCGGATGGAGTGGCGGGCACCCTGCTCACCCTGTCCGCCGCGGGCAGCACCCTGCTGATGGCGCGCTATGCCCT
GCTGGTGGCCCGCAGCGGCACGAAAGGCACGCCGCCGCTCGGCGTCGTTGTGCCCGCGCTCGCCCTCGCCGTTCTGGCGC
TGGTTTTGCCGTTTGCGCTGTTCGAGGCGGTGACGGGCCTGCCCGCATCGGCGGCCTTCACGCCATCGGCACTCCTGAAG
GGGCTGTGGCCCGTGGCGCTCGGCCTCGGGGGCGCGGCGCTCCTGGCCTGGAGGGCGCCGCGCATGCGGGAGGTGCCGGC
CGGCGACATCCTCAACGCCCTCGGCTTTCTGGGCACTGTGGGCCGAGCGATCATGGGCGGTGCTGTGGCGGCTGACGACG
TGCTGCGCCGCTGGCCGGTCGCCGCCTTCGCCCTGCTCGCGATCGCCGCCCTGCTCGCCGCCACGCTGACCTTCGCCGCC
CTGCCTTTATAG

Upstream 100 bases:

>100_bases
TCGCCCTCGCCTTCGGGGCCATGCTGGTGGGCTTCATGCCGCTCGCTCCCCTGCCCTTCCTGGAGATCGGCCGCCCCCTG
ACCTGGACGGGAGTGATGCC

Downstream 100 bases:

>100_bases
CGAGCCCCGCCCATGGCAGGGTTCACATGACCGAATCGGTCGACCTGAGGCGGGAAGGCGTTGGCGTTCGGCCGCCGACG
CACAAGACGAGTGAGGGAAC

Product: NADH/ubiquinone/plastoquinone (complex I)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MTGETLSPPLDLSALEPAMPALSEALMVVAIALPLVLAFAALVPAVRRALPHLLVFVPLPALIAALAVPRGLSMLVAPDP
FRFTLALDAPGALLLGGASLLWMTAGTYAMAYLRKDPARPAFMVWWLLTMAGSFAVFLVADVASFYLAYALVSFSAYGLA
AHDDTPQARRAGSLYLALTVLGEALLIAAFVMLASGATDANPQIRDAVALLPTSPWKTPVLVLILLGFILKMGLFPLHVW
MPLAHSVAPVPGSAVLSGVVVKAGVIGLLRFLPEGVPLPGWGAMLCGAGLFTAYYGVAVGFTQTHPKRALAYSTVSQMGV
VAAVIGAGVMNGTAGTFTVAALYAANHMLLKGAMFLAVGVAMASSPKRAWAVAALAGMLGLSLAGLPLTGGAMAKYAIKL
IVPDGVAGTLLTLSAAGSTLLMARYALLVARSGTKGTPPLGVVVPALALAVLALVLPFALFEAVTGLPASAAFTPSALLK
GLWPVALGLGGAALLAWRAPRMREVPAGDILNALGFLGTVGRAIMGGAVAADDVLRRWPVAAFALLAIAALLAATLTFAA
LPL

Sequences:

>Translated_563_residues
MTGETLSPPLDLSALEPAMPALSEALMVVAIALPLVLAFAALVPAVRRALPHLLVFVPLPALIAALAVPRGLSMLVAPDP
FRFTLALDAPGALLLGGASLLWMTAGTYAMAYLRKDPARPAFMVWWLLTMAGSFAVFLVADVASFYLAYALVSFSAYGLA
AHDDTPQARRAGSLYLALTVLGEALLIAAFVMLASGATDANPQIRDAVALLPTSPWKTPVLVLILLGFILKMGLFPLHVW
MPLAHSVAPVPGSAVLSGVVVKAGVIGLLRFLPEGVPLPGWGAMLCGAGLFTAYYGVAVGFTQTHPKRALAYSTVSQMGV
VAAVIGAGVMNGTAGTFTVAALYAANHMLLKGAMFLAVGVAMASSPKRAWAVAALAGMLGLSLAGLPLTGGAMAKYAIKL
IVPDGVAGTLLTLSAAGSTLLMARYALLVARSGTKGTPPLGVVVPALALAVLALVLPFALFEAVTGLPASAAFTPSALLK
GLWPVALGLGGAALLAWRAPRMREVPAGDILNALGFLGTVGRAIMGGAVAADDVLRRWPVAAFALLAIAALLAATLTFAA
LPL
>Mature_562_residues
TGETLSPPLDLSALEPAMPALSEALMVVAIALPLVLAFAALVPAVRRALPHLLVFVPLPALIAALAVPRGLSMLVAPDPF
RFTLALDAPGALLLGGASLLWMTAGTYAMAYLRKDPARPAFMVWWLLTMAGSFAVFLVADVASFYLAYALVSFSAYGLAA
HDDTPQARRAGSLYLALTVLGEALLIAAFVMLASGATDANPQIRDAVALLPTSPWKTPVLVLILLGFILKMGLFPLHVWM
PLAHSVAPVPGSAVLSGVVVKAGVIGLLRFLPEGVPLPGWGAMLCGAGLFTAYYGVAVGFTQTHPKRALAYSTVSQMGVV
AAVIGAGVMNGTAGTFTVAALYAANHMLLKGAMFLAVGVAMASSPKRAWAVAALAGMLGLSLAGLPLTGGAMAKYAIKLI
VPDGVAGTLLTLSAAGSTLLMARYALLVARSGTKGTPPLGVVVPALALAVLALVLPFALFEAVTGLPASAAFTPSALLKG
LWPVALGLGGAALLAWRAPRMREVPAGDILNALGFLGTVGRAIMGGAVAADDVLRRWPVAAFALLAIAALLAATLTFAAL
PL

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Escherichia coli, GI1788827, Length=300, Percent_Identity=30.6666666666667, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788831, Length=334, Percent_Identity=28.1437125748503, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788613, Length=298, Percent_Identity=28.1879194630872, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 57436; Mature: 57304

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGETLSPPLDLSALEPAMPALSEALMVVAIALPLVLAFAALVPAVRRALPHLLVFVPLP
CCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIAALAVPRGLSMLVAPDPFRFTLALDAPGALLLGGASLLWMTAGTYAMAYLRKDPARP
HHHHHHHCCCCCEEEECCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCH
AFMVWWLLTMAGSFAVFLVADVASFYLAYALVSFSAYGLAAHDDTPQARRAGSLYLALTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHCCCHHHHHHH
LGEALLIAAFVMLASGATDANPQIRDAVALLPTSPWKTPVLVLILLGFILKMGLFPLHVW
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MPLAHSVAPVPGSAVLSGVVVKAGVIGLLRFLPEGVPLPGWGAMLCGAGLFTAYYGVAVG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
FTQTHPKRALAYSTVSQMGVVAAVIGAGVMNGTAGTFTVAALYAANHMLLKGAMFLAVGV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AMASSPKRAWAVAALAGMLGLSLAGLPLTGGAMAKYAIKLIVPDGVAGTLLTLSAAGSTL
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHCCHHH
LMARYALLVARSGTKGTPPLGVVVPALALAVLALVLPFALFEAVTGLPASAAFTPSALLK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GLWPVALGLGGAALLAWRAPRMREVPAGDILNALGFLGTVGRAIMGGAVAADDVLRRWPV
HHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AAFALLAIAALLAATLTFAALPL
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGETLSPPLDLSALEPAMPALSEALMVVAIALPLVLAFAALVPAVRRALPHLLVFVPLP
CCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIAALAVPRGLSMLVAPDPFRFTLALDAPGALLLGGASLLWMTAGTYAMAYLRKDPARP
HHHHHHHCCCCCEEEECCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCH
AFMVWWLLTMAGSFAVFLVADVASFYLAYALVSFSAYGLAAHDDTPQARRAGSLYLALTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHCCCHHHHHHH
LGEALLIAAFVMLASGATDANPQIRDAVALLPTSPWKTPVLVLILLGFILKMGLFPLHVW
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MPLAHSVAPVPGSAVLSGVVVKAGVIGLLRFLPEGVPLPGWGAMLCGAGLFTAYYGVAVG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
FTQTHPKRALAYSTVSQMGVVAAVIGAGVMNGTAGTFTVAALYAANHMLLKGAMFLAVGV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AMASSPKRAWAVAALAGMLGLSLAGLPLTGGAMAKYAIKLIVPDGVAGTLLTLSAAGSTL
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHCCHHH
LMARYALLVARSGTKGTPPLGVVVPALALAVLALVLPFALFEAVTGLPASAAFTPSALLK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GLWPVALGLGGAALLAWRAPRMREVPAGDILNALGFLGTVGRAIMGGAVAADDVLRRWPV
HHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AAFALLAIAALLAATLTFAALPL
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]