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The map label for this gene is cysT [H]

Identifier: 154245340

GI number: 154245340

Start: 1597272

End: 1598126

Strand: Reverse

Name: cysT [H]

Synonym: Xaut_1393

Alternate gene names: 154245340

Gene position: 1598126-1597272 (Counterclockwise)

Preceding gene: 154245341

Following gene: 154245339

Centisome position: 30.1

GC content: 67.84

Gene sequence:

>855_bases
ATGAGCGCCTCTTCGCCCCGCTTCGCCTTCAGGCAGCAGAGCGTCATCCCCGGCTTCGGCCTCACCATGGGGCTGACGCT
CACCTGGCTTGGCCTCATCGTGCTGCTGCCCCTGTCGGCCCTGTTCATCAAGACCAGCGAGCTCACGTTCGAGCGCTTCA
TCGACATCGTCACCGCCGCCCGCACCCTGCACGCCTTGCAGGTGTCGTTCGGGCTGGCGCTGGTGGCGGCGCTGGTGAAC
GTGGTGTTCGGCGCCATCGTGGTGTGGGTGCTGGTGCGCTACGAGTTTCCCTTCAAGAAGGTGGTGGACGCCCTCATCGA
CATCCCCTTCGCCCTGCCCACCGCCGTGGCGGGCATCGCGCTGACCGCGCTCTATGCCGAAAACGGATGGGTCGGCTCCC
TGCTGGCGCCGCTCGGCATAAAGGTGTCGTTCACGCCTCTGGGCATCGTGGTGGCCCTCGTCTTCATCGGTCTGCCGTTC
GTGGTGCGCACCGTGCAGCCCGTGCTCGCGGACCTCGACCAGGAGCTGGAGGAAGCCGCCGCCAGCCTCGGCGCCACGCG
CACCCAGACCCTCGTCAAGGTGGTGCTGCCCTCCATCTGGCCGGCGCTGCTCACCGGCTTCGCCCTCGCCTTCGCGCGGG
CGGTGGGCGAATACGGCTCCGTCATCTTCATCGCCGGCAACCTGCCGGGGGTGTCGGAGATCGCGCCTCTGCTCATCGTG
ATCCGGCTGGAGGAGTTCCGCTATGCGGATGCCACCGCCATCGCCACCGTGATGCTGGTGGCCTCGTTCCTGCTCCTGCT
GATCATCAACCGCCTGCAGCGCTGGGCGGAGCTGCGGGGGAGCCGCAACGCATGA

Upstream 100 bases:

>100_bases
TGATCCGTTTCCTTATCCTCGCGGCCGCCGTCCCCTCCCGGGCGGCGGCCGTTTTCCGGACCCGAGAGACGGGACCTTCG
ACAGAGTTTATGATTTGCCC

Downstream 100 bases:

>100_bases
GCACGCTCGGCCAGCCGGTCCAGACCACGATCCACGGTGCCAGCGAGGGCCTGCCCTCCGCGCTTGAGCAATCCCATGCC
TTTGCCCACCACGGCGGCCG

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSASSPRFAFRQQSVIPGFGLTMGLTLTWLGLIVLLPLSALFIKTSELTFERFIDIVTAARTLHALQVSFGLALVAALVN
VVFGAIVVWVLVRYEFPFKKVVDALIDIPFALPTAVAGIALTALYAENGWVGSLLAPLGIKVSFTPLGIVVALVFIGLPF
VVRTVQPVLADLDQELEEAAASLGATRTQTLVKVVLPSIWPALLTGFALAFARAVGEYGSVIFIAGNLPGVSEIAPLLIV
IRLEEFRYADATAIATVMLVASFLLLLIINRLQRWAELRGSRNA

Sequences:

>Translated_284_residues
MSASSPRFAFRQQSVIPGFGLTMGLTLTWLGLIVLLPLSALFIKTSELTFERFIDIVTAARTLHALQVSFGLALVAALVN
VVFGAIVVWVLVRYEFPFKKVVDALIDIPFALPTAVAGIALTALYAENGWVGSLLAPLGIKVSFTPLGIVVALVFIGLPF
VVRTVQPVLADLDQELEEAAASLGATRTQTLVKVVLPSIWPALLTGFALAFARAVGEYGSVIFIAGNLPGVSEIAPLLIV
IRLEEFRYADATAIATVMLVASFLLLLIINRLQRWAELRGSRNA
>Mature_283_residues
SASSPRFAFRQQSVIPGFGLTMGLTLTWLGLIVLLPLSALFIKTSELTFERFIDIVTAARTLHALQVSFGLALVAALVNV
VFGAIVVWVLVRYEFPFKKVVDALIDIPFALPTAVAGIALTALYAENGWVGSLLAPLGIKVSFTPLGIVVALVFIGLPFV
VRTVQPVLADLDQELEEAAASLGATRTQTLVKVVLPSIWPALLTGFALAFARAVGEYGSVIFIAGNLPGVSEIAPLLIVI
RLEEFRYADATAIATVMLVASFLLLLIINRLQRWAELRGSRNA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=266, Percent_Identity=50.3759398496241, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082099, Length=250, Percent_Identity=31.2, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1786980, Length=174, Percent_Identity=33.9080459770115, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787369, Length=273, Percent_Identity=26.3736263736264, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786254, Length=218, Percent_Identity=26.605504587156, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787368, Length=219, Percent_Identity=29.6803652968037, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30487; Mature: 30356

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASSPRFAFRQQSVIPGFGLTMGLTLTWLGLIVLLPLSALFIKTSELTFERFIDIVTAA
CCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTLHALQVSFGLALVAALVNVVFGAIVVWVLVRYEFPFKKVVDALIDIPFALPTAVAGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
LTALYAENGWVGSLLAPLGIKVSFTPLGIVVALVFIGLPFVVRTVQPVLADLDQELEEAA
HHHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLGATRTQTLVKVVLPSIWPALLTGFALAFARAVGEYGSVIFIAGNLPGVSEIAPLLIV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
IRLEEFRYADATAIATVMLVASFLLLLIINRLQRWAELRGSRNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SASSPRFAFRQQSVIPGFGLTMGLTLTWLGLIVLLPLSALFIKTSELTFERFIDIVTAA
CCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTLHALQVSFGLALVAALVNVVFGAIVVWVLVRYEFPFKKVVDALIDIPFALPTAVAGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
LTALYAENGWVGSLLAPLGIKVSFTPLGIVVALVFIGLPFVVRTVQPVLADLDQELEEAA
HHHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLGATRTQTLVKVVLPSIWPALLTGFALAFARAVGEYGSVIFIAGNLPGVSEIAPLLIV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
IRLEEFRYADATAIATVMLVASFLLLLIINRLQRWAELRGSRNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]