Click here to switch to the map view.

The map label for this gene is cysW [H]

Identifier: 154245339

GI number: 154245339

Start: 1596352

End: 1597275

Strand: Reverse

Name: cysW [H]

Synonym: Xaut_1392

Alternate gene names: 154245339

Gene position: 1597275-1596352 (Counterclockwise)

Preceding gene: 154245340

Following gene: 154245338

Centisome position: 30.09

GC content: 66.45

Gene sequence:

>924_bases
ATGAGCACGCTCGGCCAGCCGGTCCAGACCACGATCCACGGTGCCAGCGAGGGCCTGCCCTCCGCGCTTGAGCAATCCCA
TGCCTTTGCCCACCACGGCGGCCGGCCGGTGCGCACCGAACCGCGCGCCGTGCGCATCGCCCTCATCCTGGTGGCTCTGG
CCTTTATCGGGCTTTTCATCGGCCTGCCCCTGGTGAGCGTCTTCTACGAGGCGTTCCGGCGCGGCTGGGCGGCCTATGTG
GCCGCCATCATCGAGCCCGACGCCCTCGCGGCCATCCGCCTCACCCTCATCGTCGCGGCCATCTCGGTGACGGCGAACCT
GCTGTTCGGCCTCGTGGCCTCCTGGGCCATCGCCAAGTTCGAGTTTCCGGGCAAGAGCCTGCTCATCACCCTCATCGACC
TGCCCTTCTCGGTCTCACCCGTGGTGGCGGGCCTCATCTACGTGCTGCTGTTCGGTTCCCAGGGCCTGTTCGCCGCCTAT
CTCCAGGCATGGGACATCAAGATCATCTTCGCGCTGCCCGGCATCGTGCTCGCCACCATCTTCGTCACCTTCCCGTTCGT
GGCGCGCGAGCTGATCCCGCTGATGCAGGAGCAGGGCACTTCCGAGGAAGAGGCGGCCATCTCGCTCGGGGCGTCGGGCC
TCGCCACCTTCTTCCGCGTCACCCTGCCCAATGTGAAATGGGCGCTGCTCTATGGCGTCCTGCTCTGCAACGCCCGCGCC
ATGGGCGAGTTCGGGGCGGTGTCGGTGGTGTCCGGCCATGTGCGCGGGCTGACCAACACCATGCCGCTGCACATCGAGAT
CCTTTATAACGAGTACCAGTTCGTCGCCTCCTTCGCGGTGGCGTCGCTGCTGGCGCTGCTGGCCCTGGTGACTCTCATCG
CCAAGACCATCCTGGAACACCGCCTCGCCGAGGAAGACAGATGA

Upstream 100 bases:

>100_bases
CCACCGCCATCGCCACCGTGATGCTGGTGGCCTCGTTCCTGCTCCTGCTGATCATCAACCGCCTGCAGCGCTGGGCGGAG
CTGCGGGGGAGCCGCAACGC

Downstream 100 bases:

>100_bases
CCATCGACGTGGTTGGCGTCACCAAGCGCTTCAAGACCTTCACCGCCCTCGACCATGTGAACCTCACCATCCATTCCGGT
GAGCTGGTGGCTCTGCTTGG

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSTLGQPVQTTIHGASEGLPSALEQSHAFAHHGGRPVRTEPRAVRIALILVALAFIGLFIGLPLVSVFYEAFRRGWAAYV
AAIIEPDALAAIRLTLIVAAISVTANLLFGLVASWAIAKFEFPGKSLLITLIDLPFSVSPVVAGLIYVLLFGSQGLFAAY
LQAWDIKIIFALPGIVLATIFVTFPFVARELIPLMQEQGTSEEEAAISLGASGLATFFRVTLPNVKWALLYGVLLCNARA
MGEFGAVSVVSGHVRGLTNTMPLHIEILYNEYQFVASFAVASLLALLALVTLIAKTILEHRLAEEDR

Sequences:

>Translated_307_residues
MSTLGQPVQTTIHGASEGLPSALEQSHAFAHHGGRPVRTEPRAVRIALILVALAFIGLFIGLPLVSVFYEAFRRGWAAYV
AAIIEPDALAAIRLTLIVAAISVTANLLFGLVASWAIAKFEFPGKSLLITLIDLPFSVSPVVAGLIYVLLFGSQGLFAAY
LQAWDIKIIFALPGIVLATIFVTFPFVARELIPLMQEQGTSEEEAAISLGASGLATFFRVTLPNVKWALLYGVLLCNARA
MGEFGAVSVVSGHVRGLTNTMPLHIEILYNEYQFVASFAVASLLALLALVTLIAKTILEHRLAEEDR
>Mature_306_residues
STLGQPVQTTIHGASEGLPSALEQSHAFAHHGGRPVRTEPRAVRIALILVALAFIGLFIGLPLVSVFYEAFRRGWAAYVA
AIIEPDALAAIRLTLIVAAISVTANLLFGLVASWAIAKFEFPGKSLLITLIDLPFSVSPVVAGLIYVLLFGSQGLFAAYL
QAWDIKIIFALPGIVLATIFVTFPFVARELIPLMQEQGTSEEEAAISLGASGLATFFRVTLPNVKWALLYGVLLCNARAM
GEFGAVSVVSGHVRGLTNTMPLHIEILYNEYQFVASFAVASLLALLALVTLIAKTILEHRLAEEDR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=246, Percent_Identity=52.4390243902439, Blast_Score=273, Evalue=8e-75,
Organism=Escherichia coli, GI1788764, Length=230, Percent_Identity=36.0869565217391, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786980, Length=218, Percent_Identity=29.8165137614679, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32997; Mature: 32866

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00656 GLYCOSYL_HYDROL_F6_2 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLGQPVQTTIHGASEGLPSALEQSHAFAHHGGRPVRTEPRAVRIALILVALAFIGLFI
CCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GLPLVSVFYEAFRRGWAAYVAAIIEPDALAAIRLTLIVAAISVTANLLFGLVASWAIAKF
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EFPGKSLLITLIDLPFSVSPVVAGLIYVLLFGSQGLFAAYLQAWDIKIIFALPGIVLATI
CCCCHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEHHHHHHHHHHHH
FVTFPFVARELIPLMQEQGTSEEEAAISLGASGLATFFRVTLPNVKWALLYGVLLCNARA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHH
MGEFGAVSVVSGHVRGLTNTMPLHIEILYNEYQFVASFAVASLLALLALVTLIAKTILEH
CCCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAEEDR
HHHCCCC
>Mature Secondary Structure 
STLGQPVQTTIHGASEGLPSALEQSHAFAHHGGRPVRTEPRAVRIALILVALAFIGLFI
CCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GLPLVSVFYEAFRRGWAAYVAAIIEPDALAAIRLTLIVAAISVTANLLFGLVASWAIAKF
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EFPGKSLLITLIDLPFSVSPVVAGLIYVLLFGSQGLFAAYLQAWDIKIIFALPGIVLATI
CCCCHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEHHHHHHHHHHHH
FVTFPFVARELIPLMQEQGTSEEEAAISLGASGLATFFRVTLPNVKWALLYGVLLCNARA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHH
MGEFGAVSVVSGHVRGLTNTMPLHIEILYNEYQFVASFAVASLLALLALVTLIAKTILEH
CCCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAEEDR
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]