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The map label for this gene is nadE [H]

Identifier: 154245324

GI number: 154245324

Start: 1578727

End: 1580436

Strand: Direct

Name: nadE [H]

Synonym: Xaut_1377

Alternate gene names: 154245324

Gene position: 1578727-1580436 (Clockwise)

Preceding gene: 154245323

Following gene: 154245326

Centisome position: 29.74

GC content: 68.19

Gene sequence:

>1710_bases
ATGACCACCGTCCTGACCACCGGCATAGCGCCCGGCGCCCCTGAGACCGACGCTCCCGTCGACACCTTGTTCATCGCTGT
CGCGCAGCTCAACCCCACCGTGGGCGACGTGGCCGGCAATCTGGACCTTGCCCGCCGCACCAGCGCCGGCGCCCGCGATG
CGGGGGCGGACCTCGTGGTCTTTCCCGAGCTGTTCATCGCCGGCTATCCGCCCGAGGACCTGGTGCTGAAGCCGGCGTTC
CAGCAGGCCTGCCGCGCCGCCATCGAGACGCTGGCCGCCGAGACCGCTGACGGCCCGGCGCTGCTCATCGGCGCGCCGTG
GGTGGAGGAGGGCAAGCTCTACAATTCGGTGCTGCTGCTGGAAAACGGCGGCATCTCCGCCGTTCGCCACAAGGTGGACC
TGCCCAATTACGGCGTATTCGACGAGAAGCGCGTGTTCACCCCCGGCCCGCTGCCGGGGCCGATCCCGTTCAAGGGCGTG
CGCCTCGGCGTACCGATCTGCGAGGACATCTGGGGTGCGGACGTGGTGGAATGCCTCGCCGAGACTGGCGCGGAGCTGCT
CATCGTTCCCAACGGCTCGCCCTATCGCCAGAGCGTCTATGCCGAGCGCGAGAACGTGGCGGTGGCCCGCGTGGTGGAGA
GCCACCTGCCGCTGCTCTATGTGAACCAGGTGGGCGGGCAGGACGAGCTGGTATTCGACGGCGCGTCCTTCGCCCTTAAC
CCCGACCGCTTCCTGGCCCTGCAGTTTCCCGGCTTCATGGAGCGGGTGCGCATCTCCAAGTGGCAGCGCTTCGAGAACGG
CTGGAAGTGCCTCGGCGCCAATCTTGCCGACCGCATCGAGGGCGATGAGGCGGACTATGCCGCCTGCGTGCTGGGCCTGC
GCGACTATGTGAACAAGAACCGCTTCCCCGGCGTGGTGCTGGGGCTTTCTGGCGGCATCGACAGCGCCATCTGCGCCGCC
ATGGCGGTGGATGCCCTGGGGCCGGAGCGGGTCCACTGCATCATGCTGCCCTATCGTTACACGTCCGAGGAAAGCCTCGC
GGATGCCGCCGCCGTGGCGCAGGCCCTGTTCGTGCGCTACGACGTGCTGCCCATCGTTGCCGGGGTGGAGGGCATGGAAG
CCGCGCTGGCGCCCCTGTTCGAGGGCACCCAGCGCGACGTGACCGAGGAAAACCTCCAGTCCCGCGTGCGCGGCGCGCTG
CTCATGTCGGTGTCCAACAAGTTCGGCGCCATGGTGGTGACCACCGGCAACAAGTCCGAGATGTCCACCGGCTACGCCAC
CCTCTATGGCGACATGAACGGCGGCTACAATCCCATCAAGGACCTCTACAAGACCCAGGTCACCCGCCTGTCGCGCCTGC
GCAACCGCTGGAAGCCGGCCGACGCGCTCGGGCCGGACGGCGTCGTCATCCCCGACAGCCTCATCGACAAGCCGCCCACC
GCCGAATTGCGCGAGAACCAGAAGGACGAGGATTCGCTGCCGCCCTACGCGGTGCTGGACGCCATCCTCGAACGCCTGGT
GGAGCGCGAGCATTCGGTGGCGCAGATCATCGCCGACGGCTTCAACGCTGCCACCGTGACGCGGGTGGAGCGGCTTTTGA
ACATCGCCGAATACAAGCGCCGGCAGGCCGCGCCGGGGGTGAAGGTGACCGGCCGCAACTTCGGCCGCGACCGCCGCTAT
CCCATCACCAACCGCTTCCGCGAGGGGTGA

Upstream 100 bases:

>100_bases
AGCCCCTCCGGGGTGCCGCGATGGCGCCGGATGAGGCAGATTTCGCACCTGCCCTTTTCTTGCCCTTTCCCCCCTTCGCC
GCTGGGGTTAAATCCGATTC

Downstream 100 bases:

>100_bases
GGCGATAAGAACGCGTTTGGAAATTCAGAAGGGCATAACCGCAAACACCATCGTCATGCCCTGGCTTGTCGCACGCATCC
AAGCCGAACCGCTTGGGACG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MTTVLTTGIAPGAPETDAPVDTLFIAVAQLNPTVGDVAGNLDLARRTSAGARDAGADLVVFPELFIAGYPPEDLVLKPAF
QQACRAAIETLAAETADGPALLIGAPWVEEGKLYNSVLLLENGGISAVRHKVDLPNYGVFDEKRVFTPGPLPGPIPFKGV
RLGVPICEDIWGADVVECLAETGAELLIVPNGSPYRQSVYAERENVAVARVVESHLPLLYVNQVGGQDELVFDGASFALN
PDRFLALQFPGFMERVRISKWQRFENGWKCLGANLADRIEGDEADYAACVLGLRDYVNKNRFPGVVLGLSGGIDSAICAA
MAVDALGPERVHCIMLPYRYTSEESLADAAAVAQALFVRYDVLPIVAGVEGMEAALAPLFEGTQRDVTEENLQSRVRGAL
LMSVSNKFGAMVVTTGNKSEMSTGYATLYGDMNGGYNPIKDLYKTQVTRLSRLRNRWKPADALGPDGVVIPDSLIDKPPT
AELRENQKDEDSLPPYAVLDAILERLVEREHSVAQIIADGFNAATVTRVERLLNIAEYKRRQAAPGVKVTGRNFGRDRRY
PITNRFREG

Sequences:

>Translated_569_residues
MTTVLTTGIAPGAPETDAPVDTLFIAVAQLNPTVGDVAGNLDLARRTSAGARDAGADLVVFPELFIAGYPPEDLVLKPAF
QQACRAAIETLAAETADGPALLIGAPWVEEGKLYNSVLLLENGGISAVRHKVDLPNYGVFDEKRVFTPGPLPGPIPFKGV
RLGVPICEDIWGADVVECLAETGAELLIVPNGSPYRQSVYAERENVAVARVVESHLPLLYVNQVGGQDELVFDGASFALN
PDRFLALQFPGFMERVRISKWQRFENGWKCLGANLADRIEGDEADYAACVLGLRDYVNKNRFPGVVLGLSGGIDSAICAA
MAVDALGPERVHCIMLPYRYTSEESLADAAAVAQALFVRYDVLPIVAGVEGMEAALAPLFEGTQRDVTEENLQSRVRGAL
LMSVSNKFGAMVVTTGNKSEMSTGYATLYGDMNGGYNPIKDLYKTQVTRLSRLRNRWKPADALGPDGVVIPDSLIDKPPT
AELRENQKDEDSLPPYAVLDAILERLVEREHSVAQIIADGFNAATVTRVERLLNIAEYKRRQAAPGVKVTGRNFGRDRRY
PITNRFREG
>Mature_568_residues
TTVLTTGIAPGAPETDAPVDTLFIAVAQLNPTVGDVAGNLDLARRTSAGARDAGADLVVFPELFIAGYPPEDLVLKPAFQ
QACRAAIETLAAETADGPALLIGAPWVEEGKLYNSVLLLENGGISAVRHKVDLPNYGVFDEKRVFTPGPLPGPIPFKGVR
LGVPICEDIWGADVVECLAETGAELLIVPNGSPYRQSVYAERENVAVARVVESHLPLLYVNQVGGQDELVFDGASFALNP
DRFLALQFPGFMERVRISKWQRFENGWKCLGANLADRIEGDEADYAACVLGLRDYVNKNRFPGVVLGLSGGIDSAICAAM
AVDALGPERVHCIMLPYRYTSEESLADAAAVAQALFVRYDVLPIVAGVEGMEAALAPLFEGTQRDVTEENLQSRVRGALL
MSVSNKFGAMVVTTGNKSEMSTGYATLYGDMNGGYNPIKDLYKTQVTRLSRLRNRWKPADALGPDGVVIPDSLIDKPPTA
ELRENQKDEDSLPPYAVLDAILERLVEREHSVAQIIADGFNAATVTRVERLLNIAEYKRRQAAPGVKVTGRNFGRDRRYP
ITNRFREG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61659; Mature: 61528

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVLTTGIAPGAPETDAPVDTLFIAVAQLNPTVGDVAGNLDLARRTSAGARDAGADLVV
CCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHCCCCCCCCCCCEEE
FPELFIAGYPPEDLVLKPAFQQACRAAIETLAAETADGPALLIGAPWVEEGKLYNSVLLL
ECHHHHCCCCCHHCEECHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEE
ENGGISAVRHKVDLPNYGVFDEKRVFTPGPLPGPIPFKGVRLGVPICEDIWGADVVECLA
ECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCHHHHHHHH
ETGAELLIVPNGSPYRQSVYAERENVAVARVVESHLPLLYVNQVGGQDELVFDGASFALN
HCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCEEEC
PDRFLALQFPGFMERVRISKWQRFENGWKCLGANLADRIEGDEADYAACVLGLRDYVNKN
CCCEEEEECCCHHHHHHHHHHHHHHCCHHEECCCHHHHCCCCCCHHHHHHHHHHHHHCCC
RFPGVVLGLSGGIDSAICAAMAVDALGPERVHCIMLPYRYTSEESLADAAAVAQALFVRY
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DVLPIVAGVEGMEAALAPLFEGTQRDVTEENLQSRVRGALLMSVSNKFGAMVVTTGNKSE
HHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCC
MSTGYATLYGDMNGGYNPIKDLYKTQVTRLSRLRNRWKPADALGPDGVVIPDSLIDKPPT
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHCCCCCC
AELRENQKDEDSLPPYAVLDAILERLVEREHSVAQIIADGFNAATVTRVERLLNIAEYKR
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RQAAPGVKVTGRNFGRDRRYPITNRFREG
HHCCCCCEEECCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TTVLTTGIAPGAPETDAPVDTLFIAVAQLNPTVGDVAGNLDLARRTSAGARDAGADLVV
CEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHCCCCCCCCCCCEEE
FPELFIAGYPPEDLVLKPAFQQACRAAIETLAAETADGPALLIGAPWVEEGKLYNSVLLL
ECHHHHCCCCCHHCEECHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEE
ENGGISAVRHKVDLPNYGVFDEKRVFTPGPLPGPIPFKGVRLGVPICEDIWGADVVECLA
ECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCHHHHHHHH
ETGAELLIVPNGSPYRQSVYAERENVAVARVVESHLPLLYVNQVGGQDELVFDGASFALN
HCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCEEEC
PDRFLALQFPGFMERVRISKWQRFENGWKCLGANLADRIEGDEADYAACVLGLRDYVNKN
CCCEEEEECCCHHHHHHHHHHHHHHCCHHEECCCHHHHCCCCCCHHHHHHHHHHHHHCCC
RFPGVVLGLSGGIDSAICAAMAVDALGPERVHCIMLPYRYTSEESLADAAAVAQALFVRY
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DVLPIVAGVEGMEAALAPLFEGTQRDVTEENLQSRVRGALLMSVSNKFGAMVVTTGNKSE
HHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCC
MSTGYATLYGDMNGGYNPIKDLYKTQVTRLSRLRNRWKPADALGPDGVVIPDSLIDKPPT
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHCCCCCC
AELRENQKDEDSLPPYAVLDAILERLVEREHSVAQIIADGFNAATVTRVERLLNIAEYKR
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RQAAPGVKVTGRNFGRDRRYPITNRFREG
HHCCCCCEEECCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]