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The map label for this gene is livJ [C]

Identifier: 154245299

GI number: 154245299

Start: 1553724

End: 1555022

Strand: Direct

Name: livJ [C]

Synonym: Xaut_1352

Alternate gene names: 154245299

Gene position: 1553724-1555022 (Clockwise)

Preceding gene: 154245298

Following gene: 154245300

Centisome position: 29.27

GC content: 65.13

Gene sequence:

>1299_bases
ATGTTGCTTCGTCGCCTCGTCGCGGCAGCGGGACTGTCGCTGCTCGGCCTTTGTCCCGCCCTGGCCCAGCAAAAGCCGGC
CGAGCTGAAGATCGGCATCACCACCTACAGCTCGGGCGCGGCGTCCGTCTTCGGCATCCCGGCCCGCGACGCGGCGGAGA
TGCTGGCCGCCGACATCAACGCCAAGGGTGGCGTCCAGGGCGTGCCGGTGAAGCTGTTCTTCGTGGATGAAGGCGCCGGC
ATCGAGACCCTCATGACCGAATATCGCCGCCTGGTGGAGGACACCAAGGTGGACGTGATGTTCGCCTCCATCTCCTCCGG
CGTGTGCAACAAGATCGCCCCGCTGGCCGAGGACCTGAAGGTTCTCAACTTCATGTGGGATTGCGGCACCCAGCGCATCC
TCGAGGAAGACAAGTACAATTACGTCTTCCGCACCCAGGCGAACGCGACCCCCGAGGTCCTCGCCCCGCTGCTCTATCTC
CTGAAGACCAAGCCGGACTTCAAGACCATTGCCGTGGTGAACCAGGACTATGCCTGGGGCCGCGACAGCTGGGCCATCTT
CTCCCAGGCGCTGAAGGTGCTGAAGCCCGACGTGAAGGTGGTGGCGGAATTCTTCCCCAAGTTCGGCGCCGCCGACTTCT
CCACCGAGGTCTCGCGCCTGCTGGCGCTGAAGCCGGATGTGATCTTGTCCACTTCCTGGGGCGGCGACCTCGACACCTTC
GTGCGGCAGGCCAACCAGCGCGGGCTGTTCAAGTCGTCCCTGTTTGTGCTGCCGCTGGCGGAATCCTCGCTCCAGCGCCT
CGGCACCGACCTGCCTGAGGGGGTGATCGTGGGCGGCCGCGGCGACCATTATTTCCTTCATCCCGAGCGCATCGGCGACG
CCAAGTTCAAGGCGTTCATGGACGCCTTCAAGCAGAAGACCGGCAGCTATCCCATCTACCCGGTGTTCCACATGGCGCAG
GCCTTCGCCGCCCTCGAAGCGGTCTATGGCAAGGCGGTGGCCGCCAACGGCGGCAAGTGGCCGAGCCACGACCAGGTCGC
CAAGGCGATGCCCGGCCTCACCTTCCAGGCCCTTGGCCGGCCAGTGGAAATCCGCGCCGACGGGCAGGGCGTCGAGGACC
AGCTCATCGGCACCACCACCCGCGTGCCGGCCTACAGCTTCGACATCCTCGACAACATGATGATCTTCCCCGCCGTGCAA
CTGATGCCGCCGGTGGGCCAGAAGTCCGAGGCCTGGATCGCAACGCTGAAGCCCGGCATCGTCGACATCAAGGTCGACAC
CTTCAAGGCGGCCAAATAG

Upstream 100 bases:

>100_bases
AGTCTTTTGAGATAGCCGTCGGCGAAACCAGAATGCGCTCGCCTCCCCCGGTGGGGAGAGAAGAAACGCGGTCGAATCAG
ACCGACCTGGAGGAACGCCG

Downstream 100 bases:

>100_bases
GCCCCCGATCCAAGCCATCCGAGGTTTTTCCGGGCGCGGCATGTCACGTGCCGCGCCGGTACCGCAAGGGGCCTTCATGT
CCTTCGACACCATCGTGCTG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: ABC Transporter Periplasmic Protein; Amino Acid ABC Transporter; Nitrile Hydratase Regulator; PAS Fold Family; Amino Acid ABC Transporter Periplasmic Protein; ABC-Type Branched-Chain Amino Acid Transport Systems; Branched-Chain Amino Acid ABC Transporter Permease Protein; ATPase Histidine Kinase- DNA Gyrase B- And; Signal Protein In Twin-Arginine Translocation Pathway

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLLRRLVAAAGLSLLGLCPALAQQKPAELKIGITTYSSGAASVFGIPARDAAEMLAADINAKGGVQGVPVKLFFVDEGAG
IETLMTEYRRLVEDTKVDVMFASISSGVCNKIAPLAEDLKVLNFMWDCGTQRILEEDKYNYVFRTQANATPEVLAPLLYL
LKTKPDFKTIAVVNQDYAWGRDSWAIFSQALKVLKPDVKVVAEFFPKFGAADFSTEVSRLLALKPDVILSTSWGGDLDTF
VRQANQRGLFKSSLFVLPLAESSLQRLGTDLPEGVIVGGRGDHYFLHPERIGDAKFKAFMDAFKQKTGSYPIYPVFHMAQ
AFAALEAVYGKAVAANGGKWPSHDQVAKAMPGLTFQALGRPVEIRADGQGVEDQLIGTTTRVPAYSFDILDNMMIFPAVQ
LMPPVGQKSEAWIATLKPGIVDIKVDTFKAAK

Sequences:

>Translated_432_residues
MLLRRLVAAAGLSLLGLCPALAQQKPAELKIGITTYSSGAASVFGIPARDAAEMLAADINAKGGVQGVPVKLFFVDEGAG
IETLMTEYRRLVEDTKVDVMFASISSGVCNKIAPLAEDLKVLNFMWDCGTQRILEEDKYNYVFRTQANATPEVLAPLLYL
LKTKPDFKTIAVVNQDYAWGRDSWAIFSQALKVLKPDVKVVAEFFPKFGAADFSTEVSRLLALKPDVILSTSWGGDLDTF
VRQANQRGLFKSSLFVLPLAESSLQRLGTDLPEGVIVGGRGDHYFLHPERIGDAKFKAFMDAFKQKTGSYPIYPVFHMAQ
AFAALEAVYGKAVAANGGKWPSHDQVAKAMPGLTFQALGRPVEIRADGQGVEDQLIGTTTRVPAYSFDILDNMMIFPAVQ
LMPPVGQKSEAWIATLKPGIVDIKVDTFKAAK
>Mature_432_residues
MLLRRLVAAAGLSLLGLCPALAQQKPAELKIGITTYSSGAASVFGIPARDAAEMLAADINAKGGVQGVPVKLFFVDEGAG
IETLMTEYRRLVEDTKVDVMFASISSGVCNKIAPLAEDLKVLNFMWDCGTQRILEEDKYNYVFRTQANATPEVLAPLLYL
LKTKPDFKTIAVVNQDYAWGRDSWAIFSQALKVLKPDVKVVAEFFPKFGAADFSTEVSRLLALKPDVILSTSWGGDLDTF
VRQANQRGLFKSSLFVLPLAESSLQRLGTDLPEGVIVGGRGDHYFLHPERIGDAKFKAFMDAFKQKTGSYPIYPVFHMAQ
AFAALEAVYGKAVAANGGKWPSHDQVAKAMPGLTFQALGRPVEIRADGQGVEDQLIGTTTRVPAYSFDILDNMMIFPAVQ
LMPPVGQKSEAWIATLKPGIVDIKVDTFKAAK

Specific function: This Protein Is A Component Of The Leucine, Isoleucine, Valine, (Threonine) Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994941, Length=273, Percent_Identity=26.007326007326, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 47020; Mature: 47020

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRRLVAAAGLSLLGLCPALAQQKPAELKIGITTYSSGAASVFGIPARDAAEMLAADIN
CHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHHHCC
AKGGVQGVPVKLFFVDEGAGIETLMTEYRRLVEDTKVDVMFASISSGVCNKIAPLAEDLK
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHH
VLNFMWDCGTQRILEEDKYNYVFRTQANATPEVLAPLLYLLKTKPDFKTIAVVNQDYAWG
HHHHHHHCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
RDSWAIFSQALKVLKPDVKVVAEFFPKFGAADFSTEVSRLLALKPDVILSTSWGGDLDTF
CCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHH
VRQANQRGLFKSSLFVLPLAESSLQRLGTDLPEGVIVGGRGDHYFLHPERIGDAKFKAFM
HHHHHCCCCCCCCEEEEECCHHHHHHHCCCCCCCEEECCCCCEEEECHHHHCHHHHHHHH
DAFKQKTGSYPIYPVFHMAQAFAALEAVYGKAVAANGGKWPSHDQVAKAMPGLTFQALGR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCCCCHHHCCC
PVEIRADGQGVEDQLIGTTTRVPAYSFDILDNMMIFPAVQLMPPVGQKSEAWIATLKPGI
CEEEEECCCCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCE
VDIKVDTFKAAK
EEEEECCCCCCC
>Mature Secondary Structure
MLLRRLVAAAGLSLLGLCPALAQQKPAELKIGITTYSSGAASVFGIPARDAAEMLAADIN
CHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHHHCC
AKGGVQGVPVKLFFVDEGAGIETLMTEYRRLVEDTKVDVMFASISSGVCNKIAPLAEDLK
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHH
VLNFMWDCGTQRILEEDKYNYVFRTQANATPEVLAPLLYLLKTKPDFKTIAVVNQDYAWG
HHHHHHHCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
RDSWAIFSQALKVLKPDVKVVAEFFPKFGAADFSTEVSRLLALKPDVILSTSWGGDLDTF
CCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHH
VRQANQRGLFKSSLFVLPLAESSLQRLGTDLPEGVIVGGRGDHYFLHPERIGDAKFKAFM
HHHHHCCCCCCCCEEEEECCHHHHHHHCCCCCCCEEECCCCCEEEECHHHHCHHHHHHHH
DAFKQKTGSYPIYPVFHMAQAFAALEAVYGKAVAANGGKWPSHDQVAKAMPGLTFQALGR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCCCCHHHCCC
PVEIRADGQGVEDQLIGTTTRVPAYSFDILDNMMIFPAVQLMPPVGQKSEAWIATLKPGI
CEEEEECCCCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCE
VDIKVDTFKAAK
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA