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The map label for this gene is nac [H]

Identifier: 154245298

GI number: 154245298

Start: 1552616

End: 1553593

Strand: Direct

Name: nac [H]

Synonym: Xaut_1351

Alternate gene names: 154245298

Gene position: 1552616-1553593 (Clockwise)

Preceding gene: 154245297

Following gene: 154245299

Centisome position: 29.25

GC content: 70.65

Gene sequence:

>978_bases
ATGGACCTGCGGCAGTTGCGCTATTTCGTGTCCATCGTCGAGGTGGGCTCCTTCTCCAAGGCCGCCCATCGCCTGCGCGT
GGCGCAGCCGGCCTTGAGCCAGCATGTGCGCAACATGGAAATCGACCTCGGCGTGGAGCTGCTGTTCCGCAGCCCGCAGG
GGGTGCGCGCCACCGAGGCGGGAGAGACGCTGGTGCGCCACGCGCGCCTCATCCTCAGCCAGATGGAGGTGGCGCGGGAG
GCGGTGCGCCGGGGCCAGAGCGAGCCCGAGGGCGAGGTGCGATTCGGCCTTCCGGGCACGGTGAGCCAGATGCTGTGCGT
GCCGCTGCTCAGCGAGGCGCGCCGTCGCTACCCCAAGATCAAGCTGCGCGTCGCCGAAGCCATGAGCGGCTTCGTGCTGG
ACTGGCTGCGCGAGGGCAAGATCGACCTCGGCGTCCTCTATCGCTCGGTGGCGGATCGCACGCTGCTCGCCCGCCGCGTC
CTCTCCGAGGAATTGTGCCTGCTGGGTCCCGCCGAGCCCATGGACCGGCCCCACCCCCCGCCGGGCGCCGTCTCCTTCGC
CGACATCGCCGACCTCACGCTGATCCTGCCCAGCCTCGGCCATGGTCTTCGCGACCTCATCGAGGAGCGGGCGCTGTCCG
AGAACGTGCACCTCAGCACCGTCATCGACATCGACACCTACGGCCAGATCAAGCTGCTGGTGGAGGGCGGGCTGGGCTAT
TCCATCCTGCCCGGCGCCGCGCTGCGGGCGGAGGTGGAGAAGGGCCTGCTGCGCACCTGGCCCATGGGCGAGCCGGTGCT
CTCGCGCGACCTGCACCTCGTCCGGCCGGCTGACCGGCCCATGTCCAATGCGGTCCGGGCCATCGAGGATCTGGCCCATG
CGACGCTTGTCCGGCTGGTGCGCGAAGGCGCGTGGCCGGCCGATCTCATCGATGACGAGGCCGGCGCGGCGGCGCTCCGG
GAGCGGGTATTGGGCTAG

Upstream 100 bases:

>100_bases
GGCGGTGCTGCTGGAAGCCGTGGGCATTTCTCCATAACTTTTTGGCGTCTGTGGGTGAAGTCCTATAAGCTTCTTGTCTA
TCAAGGGGCGAGGAAACGCC

Downstream 100 bases:

>100_bases
AACTTTCCGCTATGGGATCCAAGTCAAAATAGTCTTTTGAGATAGCCGTCGGCGAAACCAGAATGCGCTCGCCTCCCCCG
GTGGGGAGAGAAGAAACGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MDLRQLRYFVSIVEVGSFSKAAHRLRVAQPALSQHVRNMEIDLGVELLFRSPQGVRATEAGETLVRHARLILSQMEVARE
AVRRGQSEPEGEVRFGLPGTVSQMLCVPLLSEARRRYPKIKLRVAEAMSGFVLDWLREGKIDLGVLYRSVADRTLLARRV
LSEELCLLGPAEPMDRPHPPPGAVSFADIADLTLILPSLGHGLRDLIEERALSENVHLSTVIDIDTYGQIKLLVEGGLGY
SILPGAALRAEVEKGLLRTWPMGEPVLSRDLHLVRPADRPMSNAVRAIEDLAHATLVRLVREGAWPADLIDDEAGAAALR
ERVLG

Sequences:

>Translated_325_residues
MDLRQLRYFVSIVEVGSFSKAAHRLRVAQPALSQHVRNMEIDLGVELLFRSPQGVRATEAGETLVRHARLILSQMEVARE
AVRRGQSEPEGEVRFGLPGTVSQMLCVPLLSEARRRYPKIKLRVAEAMSGFVLDWLREGKIDLGVLYRSVADRTLLARRV
LSEELCLLGPAEPMDRPHPPPGAVSFADIADLTLILPSLGHGLRDLIEERALSENVHLSTVIDIDTYGQIKLLVEGGLGY
SILPGAALRAEVEKGLLRTWPMGEPVLSRDLHLVRPADRPMSNAVRAIEDLAHATLVRLVREGAWPADLIDDEAGAAALR
ERVLG
>Mature_325_residues
MDLRQLRYFVSIVEVGSFSKAAHRLRVAQPALSQHVRNMEIDLGVELLFRSPQGVRATEAGETLVRHARLILSQMEVARE
AVRRGQSEPEGEVRFGLPGTVSQMLCVPLLSEARRRYPKIKLRVAEAMSGFVLDWLREGKIDLGVLYRSVADRTLLARRV
LSEELCLLGPAEPMDRPHPPPGAVSFADIADLTLILPSLGHGLRDLIEERALSENVHLSTVIDIDTYGQIKLLVEGGLGY
SILPGAALRAEVEKGLLRTWPMGEPVLSRDLHLVRPADRPMSNAVRAIEDLAHATLVRLVREGAWPADLIDDEAGAAALR
ERVLG

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=294, Percent_Identity=31.2925170068027, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1787601, Length=224, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=31.6151202749141, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI157672245, Length=291, Percent_Identity=27.4914089347079, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788748, Length=290, Percent_Identity=29.3103448275862, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790399, Length=303, Percent_Identity=29.7029702970297, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1787806, Length=266, Percent_Identity=26.3157894736842, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082132, Length=267, Percent_Identity=27.3408239700375, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI145693105, Length=285, Percent_Identity=27.3684210526316, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1786401, Length=176, Percent_Identity=31.25, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=24.2320819112628, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788887, Length=248, Percent_Identity=26.2096774193548, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787589, Length=296, Percent_Identity=27.7027027027027, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789506, Length=243, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789639, Length=158, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789204, Length=309, Percent_Identity=24.2718446601942, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367136, Length=147, Percent_Identity=31.2925170068027, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081904, Length=178, Percent_Identity=28.0898876404494, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787530, Length=271, Percent_Identity=24.7232472324723, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789440, Length=119, Percent_Identity=31.9327731092437, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35860; Mature: 35860

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLRYFVSIVEVGSFSKAAHRLRVAQPALSQHVRNMEIDLGVELLFRSPQGVRATEA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHEECCCCCCCHHHH
GETLVRHARLILSQMEVAREAVRRGQSEPEGEVRFGLPGTVSQMLCVPLLSEARRRYPKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHH
KLRVAEAMSGFVLDWLREGKIDLGVLYRSVADRTLLARRVLSEELCLLGPAEPMDRPHPP
EEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PGAVSFADIADLTLILPSLGHGLRDLIEERALSENVHLSTVIDIDTYGQIKLLVEGGLGY
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCC
SILPGAALRAEVEKGLLRTWPMGEPVLSRDLHLVRPADRPMSNAVRAIEDLAHATLVRLV
CCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHH
REGAWPADLIDDEAGAAALRERVLG
HHCCCCHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MDLRQLRYFVSIVEVGSFSKAAHRLRVAQPALSQHVRNMEIDLGVELLFRSPQGVRATEA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHEECCCCCCCHHHH
GETLVRHARLILSQMEVAREAVRRGQSEPEGEVRFGLPGTVSQMLCVPLLSEARRRYPKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHH
KLRVAEAMSGFVLDWLREGKIDLGVLYRSVADRTLLARRVLSEELCLLGPAEPMDRPHPP
EEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PGAVSFADIADLTLILPSLGHGLRDLIEERALSENVHLSTVIDIDTYGQIKLLVEGGLGY
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCC
SILPGAALRAEVEKGLLRTWPMGEPVLSRDLHLVRPADRPMSNAVRAIEDLAHATLVRLV
CCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHH
REGAWPADLIDDEAGAAALRERVLG
HHCCCCHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]