Click here to switch to the map view.

The map label for this gene is gnuT [H]

Identifier: 154245182

GI number: 154245182

Start: 1419191

End: 1420543

Strand: Reverse

Name: gnuT [H]

Synonym: Xaut_1234

Alternate gene names: 154245182

Gene position: 1420543-1419191 (Counterclockwise)

Preceding gene: 154245183

Following gene: 154245177

Centisome position: 26.76

GC content: 67.41

Gene sequence:

>1353_bases
ATGCTGGGCATGAGCAAGGACACATTTCTACTGGTCGACGCGGCGGTGACCATCATCGGCCTCATCGTCCTCATCACGCG
CTTCAAGGTCCACCCCTTCGTGGCGTTGATCCTGGCCGCCGGCTTCCTGGGGCTCAGCTCCGGCATGCCGGTGGACCTCA
TCATGAAGTCGTTCCAGGACGGCTTCGGCGGCGTGCTCGGCTTCGTCGGCATCGTGCTCGGCCTCGGCACCATGCTGGGC
AAGCTGATGGCCGAATCGGGCGGCGCAGACCAGATCGCCCAGACCCTCATCAATGCCTTCGGCAAACAGCGGGTGCACTG
GGCCATCATGCTCGCCGCCTTCCTGGTGGGCATTCCCCTGTTCTTCGAAATCGGCTTCGTGCTGCTCATCCCGCTTGTCT
TCGTCATGGCGAGCCGCACCGGCGTGTCGCTGGTGAAGGTGGGCATTTCGCTTTTGGCGGGTCTCTCGGCGGTGCACGGC
CTGGTGCCGCCCCACCCGGGGCCGCTGCTGGCGGTGGGTGTGTTCGGCGCCGATATCGGCAAGACCATCTTCTACGGCCT
CATCGTCGGCCTGCCCGCGGCCGCCATCGCCGGCCCCATGTTCGGCTCGTTCATTTCGCGGCGCATCCCGGGCCATCCGT
CTCCGGAGCTGGTCGCCCAGTTCGTTCAGGACGAGCGCGCCCACACCCTGCCCGGCTTCGGCGTCACCCTCGCCACCATC
CTGCTGCCGGTGGTGCTGATGCTGCTGAAGGCGTTCGCCGATGTCGCCTTCGCCGCCGGCCATCCGGTGCGGGCGTGGCT
GGACCTCATCGGCCACCCCATCACCGCGCTGCTCGCGGCGCTGCTGCTGTCGCTCTACACCTTCGGCAGCGCGCGCGGCT
TCTCGCGCGAGGTCATCGGCAAGCACCTCAACGACAGCCTCCTGCCGGTGGCGGGCATCATCCTGATCGTCGGCGCCGGC
GGCGGCTTCAAGCAGATGCTGGTGGCGAGCGGCGTGGGCGATGTCATTGGCCATATGGCGGTGCAGGCCCATGTGAACCC
CATCCTCCTCGCCTGGCTGGTGGCGGCGGTGATCCGGGTGGCCACGGGCTCCGCCACGGTGGCGACCATCACCGGGGCCG
GCATCGTAGCCCCGGTCCTCGCCCTGACGCCGGGCGTCAACCGTGAGCTGCTGGTGCTGGCCACCGGCGCCGGCTCGGTG
GTGCTCTCCCACGTCAACGACGCCGGCTTCTGGCTGGTGAAGCAGTATTTCAACATGACGGTGGAGGAGACCTTCAAGAC
CTGGACCGCCATGGAGACGATCCTGTCGGTGGTGGCCCTGGGCCTGATCATGCTGCTTTCGCTGGTGGTGTAG

Upstream 100 bases:

>100_bases
CGTCAGCGAAACCTCATTGTCGTCCCGGCCGGCAACGCCGGGGCAAGAGCACCACGGACGGTCATTTTCCACTGGGTTTC
AAAAGGGTTGCGAGGAACGC

Downstream 100 bases:

>100_bases
CTCCGCCGCGACAGCACGCCCGCCGCCGCTTCCCCAAGTCCGGCGGCGGGCGTGGTTTTGTTTTGCTGCGATCGCGAGGA
CGCTGCGCGTCCGGGACTTT

Product: gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLGMSKDTFLLVDAAVTIIGLIVLITRFKVHPFVALILAAGFLGLSSGMPVDLIMKSFQDGFGGVLGFVGIVLGLGTMLG
KLMAESGGADQIAQTLINAFGKQRVHWAIMLAAFLVGIPLFFEIGFVLLIPLVFVMASRTGVSLVKVGISLLAGLSAVHG
LVPPHPGPLLAVGVFGADIGKTIFYGLIVGLPAAAIAGPMFGSFISRRIPGHPSPELVAQFVQDERAHTLPGFGVTLATI
LLPVVLMLLKAFADVAFAAGHPVRAWLDLIGHPITALLAALLLSLYTFGSARGFSREVIGKHLNDSLLPVAGIILIVGAG
GGFKQMLVASGVGDVIGHMAVQAHVNPILLAWLVAAVIRVATGSATVATITGAGIVAPVLALTPGVNRELLVLATGAGSV
VLSHVNDAGFWLVKQYFNMTVEETFKTWTAMETILSVVALGLIMLLSLVV

Sequences:

>Translated_450_residues
MLGMSKDTFLLVDAAVTIIGLIVLITRFKVHPFVALILAAGFLGLSSGMPVDLIMKSFQDGFGGVLGFVGIVLGLGTMLG
KLMAESGGADQIAQTLINAFGKQRVHWAIMLAAFLVGIPLFFEIGFVLLIPLVFVMASRTGVSLVKVGISLLAGLSAVHG
LVPPHPGPLLAVGVFGADIGKTIFYGLIVGLPAAAIAGPMFGSFISRRIPGHPSPELVAQFVQDERAHTLPGFGVTLATI
LLPVVLMLLKAFADVAFAAGHPVRAWLDLIGHPITALLAALLLSLYTFGSARGFSREVIGKHLNDSLLPVAGIILIVGAG
GGFKQMLVASGVGDVIGHMAVQAHVNPILLAWLVAAVIRVATGSATVATITGAGIVAPVLALTPGVNRELLVLATGAGSV
VLSHVNDAGFWLVKQYFNMTVEETFKTWTAMETILSVVALGLIMLLSLVV
>Mature_450_residues
MLGMSKDTFLLVDAAVTIIGLIVLITRFKVHPFVALILAAGFLGLSSGMPVDLIMKSFQDGFGGVLGFVGIVLGLGTMLG
KLMAESGGADQIAQTLINAFGKQRVHWAIMLAAFLVGIPLFFEIGFVLLIPLVFVMASRTGVSLVKVGISLLAGLSAVHG
LVPPHPGPLLAVGVFGADIGKTIFYGLIVGLPAAAIAGPMFGSFISRRIPGHPSPELVAQFVQDERAHTLPGFGVTLATI
LLPVVLMLLKAFADVAFAAGHPVRAWLDLIGHPITALLAALLLSLYTFGSARGFSREVIGKHLNDSLLPVAGIILIVGAG
GGFKQMLVASGVGDVIGHMAVQAHVNPILLAWLVAAVIRVATGSATVATITGAGIVAPVLALTPGVNRELLVLATGAGSV
VLSHVNDAGFWLVKQYFNMTVEETFKTWTAMETILSVVALGLIMLLSLVV

Specific function: Part Of The Gluconate Utilization System Gnt-I; High- Affinity Intake Of Gluconate. [C]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=444, Percent_Identity=45.4954954954955, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1790716, Length=420, Percent_Identity=42.1428571428571, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1788707, Length=442, Percent_Identity=42.9864253393665, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI2367370, Length=427, Percent_Identity=40.7494145199063, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1790776, Length=434, Percent_Identity=35.0230414746544, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI48994939, Length=431, Percent_Identity=39.6751740139211, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1789097, Length=443, Percent_Identity=34.7629796839729, Blast_Score=224, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 46688; Mature: 46688

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]