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The map label for this gene is hisS

Identifier: 154245164

GI number: 154245164

Start: 1398254

End: 1399783

Strand: Direct

Name: hisS

Synonym: Xaut_1216

Alternate gene names: 154245164

Gene position: 1398254-1399783 (Clockwise)

Preceding gene: 154245163

Following gene: 154245166

Centisome position: 26.34

GC content: 67.52

Gene sequence:

>1530_bases
ATGGCCAAAGAATTCAAGCCGAAGGCGCGGCTCCCCCGCGGCCTCGTCGACCGCACCGGCGCGGAGCTGTCCGCCACCCG
CGCCATGCTGGAGAAGATCCGCGCCGTCTATGAGCTGTACGGCTTCGAGGCGCTGGAGACCCCGGCCTTCGAATACACCG
ACGCCCTCGGCAAGTTCCTGCCGGACCTCGACCGCCCCAACGAGGGCGTGTTTTCGCTGAAGGACGACGACGAGCAGTGG
CTGTCCCTGCGCTATGACCTGACGGCGCCGCTGGCCCGCTACGTGGCCGAGCATTTCGAGCAGCTGCCCAAGCCCTACCG
CTCCTACCGGGCCGGCTACGTGTTCCGCAACGAGAAGCCCGGCCCCGGCCGCTTCCGCCAGTTCATGCAGTTCGACGCCG
ACACCGTGGGCGCCCCCTCGGTCGCCGCCGATGCCGAGATCTGCATGATGGCCGCCGACACCCTGGAGGCGGTGGGCGTG
CCCCGCGGTGCCTATGTGGTGCGCGTGAACAACCGGAAAGTGCTCGACGGGGTGATGGAGGCCATCGGCCTCGGCGGGGA
TGAAAATTTCGGCCGCCGCCTCACCGTGCTGCGCGCCATCGACAAGTTCGACAAGTTCGGCGTTGAAGGTGTCGAACTTC
TGCTCGGCCCCGGCCGCCGCGACGAGAGCGGCGACTTCACCAAGGGGGCGGGGCTGGACCAGCGCCAGAGCGCGGTCGTG
CTGGCCATGCTCACCGGCAAGGGCCTGCGCGCCACCGATGTGGGAGTTGAAGTCGAACTCGACGGTGATGTGGAACTCGC
GGCGGCTTTGGCGTCCAATTCCGCGTTCCTTGAGGGCATGGCGGAGCTGCGCGACATCGCCGCGCTGGTGGCTGCGGCGG
GCTATCAGGACCGGGTGAAGATCGATCCCAGCGTGGTGCGCGGCCTGGAATACTATACCGGCCCCGTCTACGAAGCCGAA
TTGACGTTTGAAATCAAGGATGAAGAGGGGCGCCCCGTGCGCTTCGGCTCGGTGGCCGGCGGCGGGCGCTATGACGGGCT
GGTGGCCCGTTTCCGCGGCGAGAAGGTGCCGGCCACGGGCTTCTCCATCGGTGTCTCCCGCCTGCTGGCGGCCCTGGCCT
TTTTGGGAAAAATGGAGGCGGCGCCAGAGGTTGGCCCGGTCGTGGTGCTGGTGATGGACAAGGAGCCGCACCGCCTCGCC
GACTATATGAAGATGGTCGCCGACCTGCGCGCCAACGGCATCCGGGCCGAGATGTATCTGGGCGGCGGCGGCATGAAACC
GCAGATGAAATATGCCGACCGGCGCGGCGCGCCTGCGGTTATCATTCAGGGATCAAACGAGAAATCGGCGGGCGAGGTGC
AGATCAAGGACCTCGTGGAGGGCGCGAAGATCGCCGCCTCCATTGCCGACAATGCGGAATATCGCGCCGCCCGCCCGGCC
CAGTTCGCGGTGAAGGAGGAAGACCTCGTCACCGCCATCCGCGACGTACTGGAACGCCGCGCCGCGCCCACGCTCGGGCT
CGCGGACTGA

Upstream 100 bases:

>100_bases
GAGGGGGCAAGACCTGCCCCCCCAAACTTGCCGTTCCGGCCCCCGTCCTCTAAGACGCCCCAACCCTTTTTGAAGATACC
GGGACGGGCTCCCAATCCCC

Downstream 100 bases:

>100_bases
GCGTTCCGGACCTGTGGCTGAGGGGAAGTCCCCTTGTCATCTCAATGATTTGAAAGACGGGCCGGGGTGCCTCTTCCCCG
GCCCGTCCTGTTTTCACAGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MAKEFKPKARLPRGLVDRTGAELSATRAMLEKIRAVYELYGFEALETPAFEYTDALGKFLPDLDRPNEGVFSLKDDDEQW
LSLRYDLTAPLARYVAEHFEQLPKPYRSYRAGYVFRNEKPGPGRFRQFMQFDADTVGAPSVAADAEICMMAADTLEAVGV
PRGAYVVRVNNRKVLDGVMEAIGLGGDENFGRRLTVLRAIDKFDKFGVEGVELLLGPGRRDESGDFTKGAGLDQRQSAVV
LAMLTGKGLRATDVGVEVELDGDVELAAALASNSAFLEGMAELRDIAALVAAAGYQDRVKIDPSVVRGLEYYTGPVYEAE
LTFEIKDEEGRPVRFGSVAGGGRYDGLVARFRGEKVPATGFSIGVSRLLAALAFLGKMEAAPEVGPVVVLVMDKEPHRLA
DYMKMVADLRANGIRAEMYLGGGGMKPQMKYADRRGAPAVIIQGSNEKSAGEVQIKDLVEGAKIAASIADNAEYRAARPA
QFAVKEEDLVTAIRDVLERRAAPTLGLAD

Sequences:

>Translated_509_residues
MAKEFKPKARLPRGLVDRTGAELSATRAMLEKIRAVYELYGFEALETPAFEYTDALGKFLPDLDRPNEGVFSLKDDDEQW
LSLRYDLTAPLARYVAEHFEQLPKPYRSYRAGYVFRNEKPGPGRFRQFMQFDADTVGAPSVAADAEICMMAADTLEAVGV
PRGAYVVRVNNRKVLDGVMEAIGLGGDENFGRRLTVLRAIDKFDKFGVEGVELLLGPGRRDESGDFTKGAGLDQRQSAVV
LAMLTGKGLRATDVGVEVELDGDVELAAALASNSAFLEGMAELRDIAALVAAAGYQDRVKIDPSVVRGLEYYTGPVYEAE
LTFEIKDEEGRPVRFGSVAGGGRYDGLVARFRGEKVPATGFSIGVSRLLAALAFLGKMEAAPEVGPVVVLVMDKEPHRLA
DYMKMVADLRANGIRAEMYLGGGGMKPQMKYADRRGAPAVIIQGSNEKSAGEVQIKDLVEGAKIAASIADNAEYRAARPA
QFAVKEEDLVTAIRDVLERRAAPTLGLAD
>Mature_508_residues
AKEFKPKARLPRGLVDRTGAELSATRAMLEKIRAVYELYGFEALETPAFEYTDALGKFLPDLDRPNEGVFSLKDDDEQWL
SLRYDLTAPLARYVAEHFEQLPKPYRSYRAGYVFRNEKPGPGRFRQFMQFDADTVGAPSVAADAEICMMAADTLEAVGVP
RGAYVVRVNNRKVLDGVMEAIGLGGDENFGRRLTVLRAIDKFDKFGVEGVELLLGPGRRDESGDFTKGAGLDQRQSAVVL
AMLTGKGLRATDVGVEVELDGDVELAAALASNSAFLEGMAELRDIAALVAAAGYQDRVKIDPSVVRGLEYYTGPVYEAEL
TFEIKDEEGRPVRFGSVAGGGRYDGLVARFRGEKVPATGFSIGVSRLLAALAFLGKMEAAPEVGPVVVLVMDKEPHRLAD
YMKMVADLRANGIRAEMYLGGGGMKPQMKYADRRGAPAVIIQGSNEKSAGEVQIKDLVEGAKIAASIADNAEYRAARPAQ
FAVKEEDLVTAIRDVLERRAAPTLGLAD

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=486, Percent_Identity=30.4526748971193, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI15029520, Length=485, Percent_Identity=30.7216494845361, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1788861, Length=169, Percent_Identity=30.1775147928994, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71993693, Length=501, Percent_Identity=29.5409181636727, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71993686, Length=501, Percent_Identity=29.5409181636727, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6325290, Length=490, Percent_Identity=27.1428571428571, Blast_Score=147, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24643061, Length=493, Percent_Identity=29.6146044624746, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24643059, Length=512, Percent_Identity=28.515625, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 55261; Mature: 55130

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEFKPKARLPRGLVDRTGAELSATRAMLEKIRAVYELYGFEALETPAFEYTDALGKFL
CCCCCCCHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHC
PDLDRPNEGVFSLKDDDEQWLSLRYDLTAPLARYVAEHFEQLPKPYRSYRAGYVFRNEKP
CCCCCCCCCEEEECCCCHHHHEEEECCHHHHHHHHHHHHHHCCHHHHHHCCCEEEECCCC
GPGRFRQFMQFDADTVGAPSVAADAEICMMAADTLEAVGVPRGAYVVRVNNRKVLDGVME
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
AIGLGGDENFGRRLTVLRAIDKFDKFGVEGVELLLGPGRRDESGDFTKGAGLDQRQSAVV
HHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
LAMLTGKGLRATDVGVEVELDGDVELAAALASNSAFLEGMAELRDIAALVAAAGYQDRVK
EEEECCCCCEEECCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
IDPSVVRGLEYYTGPVYEAELTFEIKDEEGRPVRFGSVAGGGRYDGLVARFRGEKVPATG
ECHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCC
FSIGVSRLLAALAFLGKMEAAPEVGPVVVLVMDKEPHRLADYMKMVADLRANGIRAEMYL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEE
GGGGMKPQMKYADRRGAPAVIIQGSNEKSAGEVQIKDLVEGAKIAASIADNAEYRAARPA
CCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCC
QFAVKEEDLVTAIRDVLERRAAPTLGLAD
HHCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AKEFKPKARLPRGLVDRTGAELSATRAMLEKIRAVYELYGFEALETPAFEYTDALGKFL
CCCCCCHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHC
PDLDRPNEGVFSLKDDDEQWLSLRYDLTAPLARYVAEHFEQLPKPYRSYRAGYVFRNEKP
CCCCCCCCCEEEECCCCHHHHEEEECCHHHHHHHHHHHHHHCCHHHHHHCCCEEEECCCC
GPGRFRQFMQFDADTVGAPSVAADAEICMMAADTLEAVGVPRGAYVVRVNNRKVLDGVME
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
AIGLGGDENFGRRLTVLRAIDKFDKFGVEGVELLLGPGRRDESGDFTKGAGLDQRQSAVV
HHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
LAMLTGKGLRATDVGVEVELDGDVELAAALASNSAFLEGMAELRDIAALVAAAGYQDRVK
EEEECCCCCEEECCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
IDPSVVRGLEYYTGPVYEAELTFEIKDEEGRPVRFGSVAGGGRYDGLVARFRGEKVPATG
ECHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCC
FSIGVSRLLAALAFLGKMEAAPEVGPVVVLVMDKEPHRLADYMKMVADLRANGIRAEMYL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEE
GGGGMKPQMKYADRRGAPAVIIQGSNEKSAGEVQIKDLVEGAKIAASIADNAEYRAARPA
CCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCC
QFAVKEEDLVTAIRDVLERRAAPTLGLAD
HHCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA