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The map label for this gene is leuC2 [H]

Identifier: 154245128

GI number: 154245128

Start: 1356098

End: 1357540

Strand: Direct

Name: leuC2 [H]

Synonym: Xaut_1180

Alternate gene names: 154245128

Gene position: 1356098-1357540 (Clockwise)

Preceding gene: 154245126

Following gene: 154245137

Centisome position: 25.54

GC content: 69.02

Gene sequence:

>1443_bases
ATGACCGCACCTGCGAACGCCGCACCCGCGAACGGCCCCCGCACCCTCTATGACAAGATCTTCGACGACCACGTGGTGGC
GCGGCAGGAGGATGGCACCTGCCTCCTCTATATCGACCGCCACCTGGTCCATGAGGTGACCAGCCCCCAGGCCTTCGAGG
GCCTGCGCATGACCGGGCGCACCGTGCGCGCGCCGCACAAGACGCTGGCGGTGGTGGACCATAACGTCCCCACCACCGAC
CGCACCCTGCCGAACCCGGACCCGGAGAGCGTGGCGCAGATCGCCGCGCTGGCGGAGAATACCCGCGAGTTCGGCGTCGA
ATATTATGACGGCTTCGACAAGCGCCAGGGCGTGGTCCATGTGGTGGGCCCCGAGCAGGGCTTCACCCTGCCCGGCACCA
CCATCGTCTGCGGCGACAGCCACACCTCCACCCACGGCGCCTTCGGGGCGCTCGCCCATGGCATCGGCACGTCCGAGGTG
GAGCATGTGCTCGCCACCCAGACGCTGATCCAGAAGAAGGCGAAGAACATGCGCGTCACCGTCTCCGGGCCTCTGCCCGA
GGGCGTTGGCGCCAAGGACGTGGTGCTCGCCATCATCGGCACCATCGGCACCGCCGGCGGCACCGGCTATGTCATCGAAT
ACGCGGGCGACGCCATCACCTCCCTGTCCATGGAAGGGCGGATGACGGTGTGCAACATGTCCATCGAGGGCGGCGCCCGC
GCCGGCCTCGTGGCCCCTGACGAGAAGGCCTTCGCCTATCTGAAGGACCGGCCCAAGTCGCCCAAGGGTGCCGACTGGGA
TGCCGCCCTGCGCTTCTGGCAGACCCTGCGCACCGACGCGGGCGCCCATTTCGACGCCGAGATCCAGCTCGACGGCGCCA
ACCTGCCGCCCATCGTCTCCTGGGGCACCAGCCCTGAGGACGTGATCTCGGTGGAGGGCCTGGTGCCTGATCCGGCCCTC
ATCGCCGACGAGGGCAAGCGCGCCGCCAAGATCCGCGCGCTGGGCTATATGGGCCTGACCGCCGGCACCAAGATCACCGA
CATCACGCTGGACCGCGTGTTCATCGGCTCGTGCACCAACGGCCGCATCGAGGATCTGCGGCAGGCCGCGGCCATCGTCG
CCGGCCACACCGTGAACGCGAATGTGAACGCCATGGTGGTGCCGGGCTCGGGCCTCGTGAAGGAGCAGGCCGAGGCGGAA
GGCCTCGACAAGATCTTCCTCGCCGCCGGCTTCGAGTGGCGCGAGCCGGGCTGCTCCATGTGCCTTGCCATGAACGCCGA
CCGCCTCGCCCCGGAAGAGCGCTGCGCCTCCACCTCGAACCGCAATTTCGAGGGCCGTCAGGGCTTCAAGGGACGCACCC
ACCTGGTGTCGCCCGCCATGGCTGCGGCTGCGGCCATCGCCGGCCACTTCGTGGATGTGCGCCACTGGCCCAAGGCGGCG
TGA

Upstream 100 bases:

>100_bases
ATTCAAGTCTCGTTGTCGAGACAGCCCGCATTCCAGATACCGGCCCGGCCGCCCATCGCGCGCGCCGGCCTCTCCTCAGG
ACAGGACGTAAGACGTAGCC

Downstream 100 bases:

>100_bases
AAGTGATGGCCTGAGGGTTTTCTCAGCCTGTTGATACGGAACGGCCGGGCTCTCGCCCGGCCGTTTTCGTTTGGGGCGCG
CTCACCCCACCCAGTCGAGG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTAPANAAPANGPRTLYDKIFDDHVVARQEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRTVRAPHKTLAVVDHNVPTTD
RTLPNPDPESVAQIAALAENTREFGVEYYDGFDKRQGVVHVVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEV
EHVLATQTLIQKKAKNMRVTVSGPLPEGVGAKDVVLAIIGTIGTAGGTGYVIEYAGDAITSLSMEGRMTVCNMSIEGGAR
AGLVAPDEKAFAYLKDRPKSPKGADWDAALRFWQTLRTDAGAHFDAEIQLDGANLPPIVSWGTSPEDVISVEGLVPDPAL
IADEGKRAAKIRALGYMGLTAGTKITDITLDRVFIGSCTNGRIEDLRQAAAIVAGHTVNANVNAMVVPGSGLVKEQAEAE
GLDKIFLAAGFEWREPGCSMCLAMNADRLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRHWPKAA

Sequences:

>Translated_480_residues
MTAPANAAPANGPRTLYDKIFDDHVVARQEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRTVRAPHKTLAVVDHNVPTTD
RTLPNPDPESVAQIAALAENTREFGVEYYDGFDKRQGVVHVVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEV
EHVLATQTLIQKKAKNMRVTVSGPLPEGVGAKDVVLAIIGTIGTAGGTGYVIEYAGDAITSLSMEGRMTVCNMSIEGGAR
AGLVAPDEKAFAYLKDRPKSPKGADWDAALRFWQTLRTDAGAHFDAEIQLDGANLPPIVSWGTSPEDVISVEGLVPDPAL
IADEGKRAAKIRALGYMGLTAGTKITDITLDRVFIGSCTNGRIEDLRQAAAIVAGHTVNANVNAMVVPGSGLVKEQAEAE
GLDKIFLAAGFEWREPGCSMCLAMNADRLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRHWPKAA
>Mature_479_residues
TAPANAAPANGPRTLYDKIFDDHVVARQEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRTVRAPHKTLAVVDHNVPTTDR
TLPNPDPESVAQIAALAENTREFGVEYYDGFDKRQGVVHVVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVE
HVLATQTLIQKKAKNMRVTVSGPLPEGVGAKDVVLAIIGTIGTAGGTGYVIEYAGDAITSLSMEGRMTVCNMSIEGGARA
GLVAPDEKAFAYLKDRPKSPKGADWDAALRFWQTLRTDAGAHFDAEIQLDGANLPPIVSWGTSPEDVISVEGLVPDPALI
ADEGKRAAKIRALGYMGLTAGTKITDITLDRVFIGSCTNGRIEDLRQAAAIVAGHTVNANVNAMVVPGSGLVKEQAEAEG
LDKIFLAAGFEWREPGCSMCLAMNADRLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRHWPKAA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=373, Percent_Identity=28.686327077748, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8659555, Length=381, Percent_Identity=25.9842519685039, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI41352693, Length=392, Percent_Identity=26.530612244898, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786259, Length=462, Percent_Identity=64.0692640692641, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=25.6267409470752, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081781, Length=347, Percent_Identity=25.0720461095101, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=376, Percent_Identity=29.7872340425532, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32564738, Length=376, Percent_Identity=29.7872340425532, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=301, Percent_Identity=29.5681063122924, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=398, Percent_Identity=27.3869346733668, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=63.3684210526316, Blast_Score=609, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6320440, Length=429, Percent_Identity=24.009324009324, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=380, Percent_Identity=29.2105263157895, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6322261, Length=483, Percent_Identity=24.6376811594203, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI281365315, Length=397, Percent_Identity=27.7078085642317, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17864292, Length=397, Percent_Identity=27.7078085642317, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161076999, Length=397, Percent_Identity=27.7078085642317, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28571643, Length=373, Percent_Identity=27.3458445040214, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645686, Length=374, Percent_Identity=28.0748663101604, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=27.2237196765499, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50891; Mature: 50760

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPANAAPANGPRTLYDKIFDDHVVARQEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGR
CCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCEEEEEECHHHHHHCCCCHHHHCHHHCCC
TVRAPHKTLAVVDHNVPTTDRTLPNPDPESVAQIAALAENTREFGVEYYDGFDKRQGVVH
EECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEE
VVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRVT
EECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEE
VSGPLPEGVGAKDVVLAIIGTIGTAGGTGYVIEYAGDAITSLSMEGRMTVCNMSIEGGAR
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEEEEECCCCC
AGLVAPDEKAFAYLKDRPKSPKGADWDAALRFWQTLRTDAGAHFDAEIQLDGANLPPIVS
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEE
WGTSPEDVISVEGLVPDPALIADEGKRAAKIRALGYMGLTAGTKITDITLDRVFIGSCTN
CCCCHHHEEEECCCCCCCCEEECCCCCEEEEEEEEEEEECCCCEEEEEEEEEEEEECCCC
GRIEDLRQAAAIVAGHTVNANVNAMVVPGSGLVKEQAEAEGLDKIFLAAGFEWREPGCSM
CHHHHHHHHHHHHCCCEECCCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCE
CLAMNADRLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRHWPKAA
EEEECCHHCCCHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TAPANAAPANGPRTLYDKIFDDHVVARQEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGR
CCCCCCCCCCCCHHHHHHHHCCCEEEECCCCEEEEEECHHHHHHCCCCHHHHCHHHCCC
TVRAPHKTLAVVDHNVPTTDRTLPNPDPESVAQIAALAENTREFGVEYYDGFDKRQGVVH
EECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEE
VVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRVT
EECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEE
VSGPLPEGVGAKDVVLAIIGTIGTAGGTGYVIEYAGDAITSLSMEGRMTVCNMSIEGGAR
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEEEEECCCCC
AGLVAPDEKAFAYLKDRPKSPKGADWDAALRFWQTLRTDAGAHFDAEIQLDGANLPPIVS
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEE
WGTSPEDVISVEGLVPDPALIADEGKRAAKIRALGYMGLTAGTKITDITLDRVFIGSCTN
CCCCHHHEEEECCCCCCCCEEECCCCCEEEEEEEEEEEECCCCEEEEEEEEEEEEECCCC
GRIEDLRQAAAIVAGHTVNANVNAMVVPGSGLVKEQAEAEGLDKIFLAAGFEWREPGCSM
CHHHHHHHHHHHHCCCEECCCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCE
CLAMNADRLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRHWPKAA
EEEECCHHCCCHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]