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The map label for this gene is bioB

Identifier: 154245065

GI number: 154245065

Start: 1287634

End: 1288671

Strand: Reverse

Name: bioB

Synonym: Xaut_1117

Alternate gene names: 154245065

Gene position: 1288671-1287634 (Counterclockwise)

Preceding gene: 154245066

Following gene: 154245062

Centisome position: 24.27

GC content: 70.23

Gene sequence:

>1038_bases
ATGACCACGAGCACCGGCACGACCACCGGGACCGGAACCGCCCGCGCGGCGGAGGGGAGCCTGCTGCGCTCCGACTGGAC
CGAGGCCGAGGCCCGTGCGCTCTACGACCTGCCCTTCCCCGAGCTGATGTTCCGCGCCCAGAGCGTGCACCGCCGCCACT
TCGATCCCACCACGGTGGAGACGGCGACGCTGCTCTCCATCAAGACCGGCGGCTGCGCCGAGGATTGCGGCTACTGCTCC
CAGAGCGCCCACCACGAGACCGGCCTGAAGGCCACCAAGCTCATGGGCGTGGAAGAGGTGCTGTCGCAGGCCCAGAAGGC
CAAGGACGGCGGCGCCACCCGCTTCTGCATGGGCGCCGCCTGGCGCAGCCCCAAGGATCGCGACCTCGACGCCGTCTGCG
CCATGATCTCGGGCGTGAAGGCCATGGGCCTTGAAGCCTGCGTGACCCTGGGCATGCTCACCCCGGATCAGGTGACCCGC
CTCGGCGCCGCCGGCCTCGACTATTACAACCACAACATCGACACCTCGCCCGCCTTCTACCCCGAGATCGTCACCACCCG
GACCATGGACGACCGCCTCGACACCCTCGCCTCGGTGCGGGCGGGCGGCATCCGGCTGTGCACCGGCGGCATCGTCGGCA
TGGGCGAGAGCGTGGACGACCGCATCGCCATGCTGCTGGTGCTCGCCACCCTCGATCCCCATCCCGAGAGCGTGCCGATC
AACATGTGGAATGAGATCGAGGGCACCCCGGTGATGGGCAAGGCCGACAAGCCGGACCCCATCGCCTTCGTGCGCCTGAT
CGCGCTGGCGCGCCTTCTGATGCCGCAGAGCATCGTGCGCCTCTCCGCCGGCCGGGACGCCATGAGCGACGAGATGCAGG
CCCTGTGCTTCCTTGCCGGGGCCAATTCCATCTTCACCGGCGACGTGCTGCTCACCACCGCCAACCCGGAGCGGGACAAG
GACGCGGCGCTGTTCGCCCGCCTCGGTATCCGCGCCGCCGCCCCCGCCGCCACGGAGATGCGCTCCGCCGCGGAGTGA

Upstream 100 bases:

>100_bases
TGAAGCCCGGGCCTGAAGGACCGACAAGGGGAGGATTGCGCCCGGTGTGCGTTGCGGCGTAATCCTTGGGCCCAGATCGG
GGAAACCTCAGGAGCAACGC

Downstream 100 bases:

>100_bases
AGGCGCGCGACGGCCGCCTCCGGACGCTCGCCGCAGGAGGCATCAGCCTTGCGCGGTCAGCCTCGCGTGGTCAGCCTTGC
ACGATCTCGGCGCTGGCGCC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTTSTGTTTGTGTARAAEGSLLRSDWTEAEARALYDLPFPELMFRAQSVHRRHFDPTTVETATLLSIKTGGCAEDCGYCS
QSAHHETGLKATKLMGVEEVLSQAQKAKDGGATRFCMGAAWRSPKDRDLDAVCAMISGVKAMGLEACVTLGMLTPDQVTR
LGAAGLDYYNHNIDTSPAFYPEIVTTRTMDDRLDTLASVRAGGIRLCTGGIVGMGESVDDRIAMLLVLATLDPHPESVPI
NMWNEIEGTPVMGKADKPDPIAFVRLIALARLLMPQSIVRLSAGRDAMSDEMQALCFLAGANSIFTGDVLLTTANPERDK
DAALFARLGIRAAAPAATEMRSAAE

Sequences:

>Translated_345_residues
MTTSTGTTTGTGTARAAEGSLLRSDWTEAEARALYDLPFPELMFRAQSVHRRHFDPTTVETATLLSIKTGGCAEDCGYCS
QSAHHETGLKATKLMGVEEVLSQAQKAKDGGATRFCMGAAWRSPKDRDLDAVCAMISGVKAMGLEACVTLGMLTPDQVTR
LGAAGLDYYNHNIDTSPAFYPEIVTTRTMDDRLDTLASVRAGGIRLCTGGIVGMGESVDDRIAMLLVLATLDPHPESVPI
NMWNEIEGTPVMGKADKPDPIAFVRLIALARLLMPQSIVRLSAGRDAMSDEMQALCFLAGANSIFTGDVLLTTANPERDK
DAALFARLGIRAAAPAATEMRSAAE
>Mature_344_residues
TTSTGTTTGTGTARAAEGSLLRSDWTEAEARALYDLPFPELMFRAQSVHRRHFDPTTVETATLLSIKTGGCAEDCGYCSQ
SAHHETGLKATKLMGVEEVLSQAQKAKDGGATRFCMGAAWRSPKDRDLDAVCAMISGVKAMGLEACVTLGMLTPDQVTRL
GAAGLDYYNHNIDTSPAFYPEIVTTRTMDDRLDTLASVRAGGIRLCTGGIVGMGESVDDRIAMLLVLATLDPHPESVPIN
MWNEIEGTPVMGKADKPDPIAFVRLIALARLLMPQSIVRLSAGRDAMSDEMQALCFLAGANSIFTGDVLLTTANPERDKD
AALFARLGIRAAAPAATEMRSAAE

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=308, Percent_Identity=58.1168831168831, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321725, Length=313, Percent_Identity=48.2428115015974, Blast_Score=318, Evalue=7e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_XANP2 (A7IEC3)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001416023.1
- ProteinModelPortal:   A7IEC3
- SMR:   A7IEC3
- STRING:   A7IEC3
- GeneID:   5421312
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_1117
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- BioCyc:   XAUT78245:XAUT_1117-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36790; Mature: 36659

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSTGTTTGTGTARAAEGSLLRSDWTEAEARALYDLPFPELMFRAQSVHRRHFDPTTVE
CCCCCCCCCCCCCCHHCCCCHHHHCCHHHHHHEEECCCCHHHHHHHHHHHHHCCCCCCCC
TATLLSIKTGGCAEDCGYCSQSAHHETGLKATKLMGVEEVLSQAQKAKDGGATRFCMGAA
CEEEEEEECCCCHHHHHHHCCCCCHHCCCHHHHHHCHHHHHHHHHHCCCCCCCEEEHHHH
WRSPKDRDLDAVCAMISGVKAMGLEACVTLGMLTPDQVTRLGAAGLDYYNHNIDTSPAFY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
PEIVTTRTMDDRLDTLASVRAGGIRLCTGGIVGMGESVDDRIAMLLVLATLDPHPESVPI
CHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
NMWNEIEGTPVMGKADKPDPIAFVRLIALARLLMPQSIVRLSAGRDAMSDEMQALCFLAG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
ANSIFTGDVLLTTANPERDKDAALFARLGIRAAAPAATEMRSAAE
CCCEEECCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure 
TTSTGTTTGTGTARAAEGSLLRSDWTEAEARALYDLPFPELMFRAQSVHRRHFDPTTVE
CCCCCCCCCCCCCHHCCCCHHHHCCHHHHHHEEECCCCHHHHHHHHHHHHHCCCCCCCC
TATLLSIKTGGCAEDCGYCSQSAHHETGLKATKLMGVEEVLSQAQKAKDGGATRFCMGAA
CEEEEEEECCCCHHHHHHHCCCCCHHCCCHHHHHHCHHHHHHHHHHCCCCCCCEEEHHHH
WRSPKDRDLDAVCAMISGVKAMGLEACVTLGMLTPDQVTRLGAAGLDYYNHNIDTSPAFY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
PEIVTTRTMDDRLDTLASVRAGGIRLCTGGIVGMGESVDDRIAMLLVLATLDPHPESVPI
CHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
NMWNEIEGTPVMGKADKPDPIAFVRLIALARLLMPQSIVRLSAGRDAMSDEMQALCFLAG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
ANSIFTGDVLLTTANPERDKDAALFARLGIRAAAPAATEMRSAAE
CCCEEECCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA