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The map label for this gene is mhpE [C]

Identifier: 154244888

GI number: 154244888

Start: 1057634

End: 1058668

Strand: Direct

Name: mhpE [C]

Synonym: Xaut_0938

Alternate gene names: 154244888

Gene position: 1057634-1058668 (Clockwise)

Preceding gene: 154244887

Following gene: 154244889

Centisome position: 19.92

GC content: 68.12

Gene sequence:

>1035_bases
ATGGCCCGTCCCAACAAAGACAAGCTCTATATCCAGGACGTGACCCTGCGCGACGGCATGCACGCGCTCAGGCACCAGTA
CGACCTTGCGGCGACGCGAGCCATCGCCCGGGCTCTCGACCGCGCCAGGGTGGACGCCATCGAGATCAGCCACGGCGACG
GCCTCACCGGCTCCACCTTCAACTACGGCTTCGGCGCCCATGACGACGCCGAGTGGATCGCCGCGGTGGCGGAGGAATGC
AGCCATGCCGTGGTCACCGTGTTGCTCATTCCGGGCATCGGCACGGTGCATGATCTCAAGCATGCCTATGAGGCCGGCGC
GCGCTCGGTGCGCGTGTGCACCCATTGCACCGAGGCTGACGTGTCGCGCCAGCACATCGAGGCAGCCCGTGAGCTGGGCA
TGGACACGGCGACCTTCCTGATGATGGCCCACATGATCCCGCCCGAGGCCCTCGCGGAGCAGGCCAGGCTCATGGAGAGC
TATGGCGCGGAATGCGTCTATGTGACGGATTCCGCCGGTGCCCTGCTGCCGGAAGACTATACGGCGCGGGTGAAGACCCT
GCGCGACGCGCTCAGGCCCGAAACCGAAATCGGCGTTCACACCCACCACAACCTCACGCTCGGCATCGCCAATGCGGTGG
CGGGTATCGAGGCCGGCGCCGTGCGCGTGGATGCCTCGCTGGCCGGCATGGGCGCCGGGGCGGGCAATGCGCCGCTGGAA
GCCCTCATCGCGGTGCTCAACCGCAAGGGCATCGACACCGGCTGTGACCTCTACGCGCTGATGGACGCCGCCGATGATCT
GGTGCGCCCGCTGCAGGACCGCCCGGTGCGGGTGGACCGGGAGAGCCTCTCGCTGGGCTATGCCGGTGTCTATTCGAGCT
TCCTGCGCCACGCGGAGAACGCCGCACAGACCTACGGCGTGGACACCCGGGAAATCCTCTCCGAACTCGGCCGCCGCCGC
ATGGTGGGCGGGCAGGAGGACATGATCGTCGACGTTGCCCTCGACCTCGCCGAGGCGAAGGCCGCCGTCGCCTGA

Upstream 100 bases:

>100_bases
ATCTGCCGGCCTATGCCGGCAACCTCGACATCATGACCTCTGCCGCGCTGGTGACCGCTGATCGCTGGGCCGCGCGCAGG
CTTGCGAAGGAGGCCGCGTG

Downstream 100 bases:

>100_bases
CAACCGCATCGCAAGTCCGGCAGGGCGCTCCCCGTTCGGGATGCGCCCTGCCGTGCCCGCGACACAACCTGAAACCGCCG
GGGATCATCGGCGGATGCAT

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MARPNKDKLYIQDVTLRDGMHALRHQYDLAATRAIARALDRARVDAIEISHGDGLTGSTFNYGFGAHDDAEWIAAVAEEC
SHAVVTVLLIPGIGTVHDLKHAYEAGARSVRVCTHCTEADVSRQHIEAARELGMDTATFLMMAHMIPPEALAEQARLMES
YGAECVYVTDSAGALLPEDYTARVKTLRDALRPETEIGVHTHHNLTLGIANAVAGIEAGAVRVDASLAGMGAGAGNAPLE
ALIAVLNRKGIDTGCDLYALMDAADDLVRPLQDRPVRVDRESLSLGYAGVYSSFLRHAENAAQTYGVDTREILSELGRRR
MVGGQEDMIVDVALDLAEAKAAVA

Sequences:

>Translated_344_residues
MARPNKDKLYIQDVTLRDGMHALRHQYDLAATRAIARALDRARVDAIEISHGDGLTGSTFNYGFGAHDDAEWIAAVAEEC
SHAVVTVLLIPGIGTVHDLKHAYEAGARSVRVCTHCTEADVSRQHIEAARELGMDTATFLMMAHMIPPEALAEQARLMES
YGAECVYVTDSAGALLPEDYTARVKTLRDALRPETEIGVHTHHNLTLGIANAVAGIEAGAVRVDASLAGMGAGAGNAPLE
ALIAVLNRKGIDTGCDLYALMDAADDLVRPLQDRPVRVDRESLSLGYAGVYSSFLRHAENAAQTYGVDTREILSELGRRR
MVGGQEDMIVDVALDLAEAKAAVA
>Mature_343_residues
ARPNKDKLYIQDVTLRDGMHALRHQYDLAATRAIARALDRARVDAIEISHGDGLTGSTFNYGFGAHDDAEWIAAVAEECS
HAVVTVLLIPGIGTVHDLKHAYEAGARSVRVCTHCTEADVSRQHIEAARELGMDTATFLMMAHMIPPEALAEQARLMESY
GAECVYVTDSAGALLPEDYTARVKTLRDALRPETEIGVHTHHNLTLGIANAVAGIEAGAVRVDASLAGMGAGAGNAPLEA
LIAVLNRKGIDTGCDLYALMDAADDLVRPLQDRPVRVDRESLSLGYAGVYSSFLRHAENAAQTYGVDTREILSELGRRRM
VGGQEDMIVDVALDLAEAKAAVA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=336, Percent_Identity=68.4523809523809, Blast_Score=456, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_XANP2 (A7IDU6)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001415846.1
- ProteinModelPortal:   A7IDU6
- SMR:   A7IDU6
- STRING:   A7IDU6
- GeneID:   5423187
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_0938
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   ATCVYVV
- ProtClustDB:   CLSK2401143
- BioCyc:   XAUT78245:XAUT_0938-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36909; Mature: 36778

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 171-171 BINDING 200-200 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPNKDKLYIQDVTLRDGMHALRHQYDLAATRAIARALDRARVDAIEISHGDGLTGSTF
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCC
NYGFGAHDDAEWIAAVAEECSHAVVTVLLIPGIGTVHDLKHAYEAGARSVRVCTHCTEAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCHHH
VSRQHIEAARELGMDTATFLMMAHMIPPEALAEQARLMESYGAECVYVTDSAGALLPEDY
HHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHH
TARVKTLRDALRPETEIGVHTHHNLTLGIANAVAGIEAGAVRVDASLAGMGAGAGNAPLE
HHHHHHHHHHHCCCCCCCCEECCCEEEEHHHHHHCCCCCCEEECHHHHCCCCCCCCCHHH
ALIAVLNRKGIDTGCDLYALMDAADDLVRPLQDRPVRVDRESLSLGYAGVYSSFLRHAEN
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHCCHHHHHHHHHHHHHH
AAQTYGVDTREILSELGRRRMVGGQEDMIVDVALDLAEAKAAVA
HHHHCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARPNKDKLYIQDVTLRDGMHALRHQYDLAATRAIARALDRARVDAIEISHGDGLTGSTF
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCC
NYGFGAHDDAEWIAAVAEECSHAVVTVLLIPGIGTVHDLKHAYEAGARSVRVCTHCTEAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCHHH
VSRQHIEAARELGMDTATFLMMAHMIPPEALAEQARLMESYGAECVYVTDSAGALLPEDY
HHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHH
TARVKTLRDALRPETEIGVHTHHNLTLGIANAVAGIEAGAVRVDASLAGMGAGAGNAPLE
HHHHHHHHHHHCCCCCCCCEECCCEEEEHHHHHHCCCCCCEEECHHHHCCCCCCCCCHHH
ALIAVLNRKGIDTGCDLYALMDAADDLVRPLQDRPVRVDRESLSLGYAGVYSSFLRHAEN
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHCCHHHHHHHHHHHHHH
AAQTYGVDTREILSELGRRRMVGGQEDMIVDVALDLAEAKAAVA
HHHHCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA